Influence of Incubation Conditions on the Nanoparticles Toxicity Based on Seed Germination and Bacterial Bioluminescence

2017 ◽  
Vol 17 (4) ◽  
pp. 2382-2389 ◽  
Author(s):  
Kyung-Seok Ko ◽  
In Chul Kong
2016 ◽  
Vol 49 (5) ◽  
pp. 411-415
Author(s):  
In Chul Kong ◽  
Mun Hee Lee ◽  
Hyun Jin Jang ◽  
Eun Jin Lee ◽  
Kyung Seok Ko ◽  
...  

2020 ◽  
Vol 21 (22) ◽  
pp. 8465
Author(s):  
In Chul Kong ◽  
Kyung-Seok Ko ◽  
Dong-Chan Koh

Seven biological methods were adopted (three bacterial activities of bioluminescence, enzyme, enzyme biosynthetic, algal growth, seed germination, and root and shoot growth) to compare the toxic effects of two different sizes of silver nanoparticles (AgNPs). AgNPs showed a different sensitivity in each bioassay. Overall, the order of inhibitory effects was roughly observed as follows; bacterial bioluminescence activity ≈ root growth > biosynthetic activity of enzymes ≈ algal growth > seed germination ≈ enzymatic activity > shoot growth. For all bacterial activities (bioluminescence, enzyme, and enzyme biosynthesis), the small AgNPs showed statistically significantly higher toxicity than the large ones (p < 0.0036), while no significant differences were observed among other biological activities. The overall effects on the biological activities (except shoot growth) of the small AgNPs were shown to have about 4.3 times lower EC50 (high toxicity) value than the large AgNPs. These results also indicated that the bacterial bioluminescence activity appeared to be an appropriate method among the tested ones in terms of both sensitivity and the discernment of particle sizes of AgNPs.


2018 ◽  
Vol 67 ◽  
pp. 198-205 ◽  
Author(s):  
In Chul Kong ◽  
Kyung Seok Ko ◽  
Mun Hui Lee ◽  
Ji Hwoan Lee ◽  
Young-Hwan Han

2020 ◽  
Vol 21 (18) ◽  
pp. 6767 ◽  
Author(s):  
In Chul Kong ◽  
Kyung-Seok Ko ◽  
Dong-Chan Koh ◽  
Chul-Min Chon

The differences in the toxicity of cobalt oxide nanoparticles (Co-NPs) of two different sizes were evaluated in the contexts of the activities of bacterial bioluminescence, xyl-lux gene, enzyme function and biosynthesis of β-galactosidase, bacterial gene mutation, algal growth, and plant seed germination and root/shoot growth. Each size of Co-NP exhibited a different level of toxicity (sensitivity) in each biological activity. No revertant mutagenic ratio (greater than 2.0) of Salmonella typhimurium TA 98 was observed under the test conditions in the case of gene-mutation experiments. Overall, the inhibitory effects on all five bacterial bioassays were greater than those on algal growth, seed germination, and root growth. However, in all cases, the small Co-NPs showed statistically greater (total average about two times) toxicity than the large Co-NPs, except in shoot growth, which showed no observable inhibition. These findings demonstrate that particle size may be an important physical factor determining the fate of Co-NPs in the environment. Moreover, combinations of results based on various biological activities and physicochemical properties, rather than only a single activity and property, would better facilitate accurate assessment of NPs’ toxicity in ecosystems.


1991 ◽  
Vol 83 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Yohji Esashi ◽  
Shinichi Matsuyama ◽  
Hiroki Ashino ◽  
Maria Ogasawara ◽  
Ryo Hasegawa

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