Mutant DNA Detection on Graphene Oxide with SYBR Green I

2016 ◽  
Vol 16 (11) ◽  
pp. 11868-11872
Author(s):  
Chunghyun Lee ◽  
Jongback Gang
Keyword(s):  
Graphene Oxide ◽  
Dna Detection ◽  
Sybr Green ◽  
Sybr Green I

Isothermal detection of RNA transcription levels using graphene oxide-based SYBR Green I fluorescence platform

2014 ◽  
Vol 25 (3) ◽  
pp. 482-484 ◽  
Author(s):  
Wen-Ge Ma ◽  
Su-Qin Lu ◽  
De-Bin Zhu ◽  
Xiao-Bo Xing ◽  
Bi-Hua Su
Keyword(s):  
Graphene Oxide ◽  
Sybr Green ◽  
Sybr Green I ◽  
Rna Transcription

FLUORESCENCE DETECTION OF MELAMINE BASE ON FUNCTIONALIZED GRAPHENE OXIDE

2020 ◽  
Vol 27 (03) ◽  
Author(s):  
VAN TRONG NGUYEN ◽  
THANH THUY TRAN ◽  
THANH KHUE VAN ◽  
NGUYEN THANH CONG
Keyword(s):  
Graphene Oxide ◽  
Linear Regression Analysis ◽  
Specific Interaction ◽  
Sybr Green ◽  
Sybr Green I ◽  
Intensity Change ◽  
Functionalized Graphene Oxide ◽  
Peak Intensity ◽  
High Throughput Method ◽  
New Strategy

In this study, we report a new strategy to detect melamine. We present a sensitive and selective fluorescent aptasensor for detection melamine based on graphene oxide (GO) and SYBR Green I. Our strategy utilizes the efficient quenching ability of GO and the different interaction intensity of aptamer, aptamer/melamine complex with GO which directly induces the fluorescence intensity change. The results of experiment showed that, with the reaction mixture consisted of 1×NEBuffer 2, 80 nM T55, 2×SG and different concentration of melamine, the fluorescence of SYBR Green I decreases as the concentration of melamine decreasing. In addition, some conditions and working curve of melamine are established with the concentration of range from 1 nM to 200 nM. Linear regression analysis of detection data yielded the following equation: y = 176.66 + 1.68 x, where y and x denoted the fluorescence peak intensity and melamine concentration, respectively. The peak intensity showed a linear correlation to the concentration of melamine in the range of 10 to 200 nM. The result further demonstrated that the fluorescence recovery was attributed to the formation of between double-stranded (dsDNA) structure by the specific interaction dsDNA and SYBR Green I (SG). Therefore, the GO-based biosensing platform is feasible to be used to selecting for detection melamine. This method provides a simple, rapid and high-throughput method for detection melamine and it could be widely applied to detect small molecules, other proteins and DNAs with specific designed oligonucleotides because of its excellent sequence-independent property.

A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 49-53 ◽  
Author(s):  
Aihua Zheng ◽  
Ming Luo ◽  
Dongshan Xiang ◽  
Xia Xiang ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Signal Amplification ◽  
Dna Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Label Free ◽  
Acid Dye ◽  
Exonuclease Iii ◽  
Amplification Assay

scholarly journals A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide

2014 ◽  
pp. 147 ◽  
Author(s):  
Shaohuang Weng ◽  
Namei Wu ◽  
Huazhang Qiu ◽  
Yanjie Zheng ◽  
Min Chen ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Sybr Green ◽  
Sybr Green I ◽  
Fluorescence Sensing

Surface-enhanced resonance Raman scattering (SERRS) simulates PCR for sensitive DNA detection

The Analyst ◽  
2015 ◽  
Vol 140 (22) ◽  
pp. 7518-7521 ◽  
Author(s):  
Haibo Zhou ◽  
Shenyu Lin ◽  
Yichu Nie ◽  
Danting Yang ◽  
Qiqin Wang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Detection Method ◽  
Dna Detection ◽  
Resonance Raman ◽  
Sybr Green ◽  
Sybr Green I ◽  
Resonance Raman Scattering ◽  
Double Stranded Dna ◽  
Surface Enhanced

This paper describes a novel double-stranded DNA detection method through resonance between SYBR Green I and DNA with the surface-enhanced resonance Raman scattering (SERRS) assay, which opens an avenue to the quantitative and reliable application of SERRS in DNA detection.

scholarly journals Highly Sensitive Detection for Mercury Ions Using Graphene Oxide (GO) Sensors

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1070
Author(s):  
Lei Liu ◽  
Haixia Shi ◽  
Raoqi Li ◽  
Cheng Liu ◽  
Jia Cheng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescent Dyes ◽  
Limit Of Detection ◽  
Sybr Green ◽  
Sybr Green I ◽  
Mercury Ions ◽  
Amino Groups ◽  
Highly Sensitive ◽  
Detection Of Signals ◽  
Highly Sensitive Detection

The mercury ion (Hg2+) is one of the heavy metal ions, and its presence in trace amounts can cause physiological damage to an organism. Traditional methods of Hg2+ detection have been useful but have also had numerous limitations and challenges, and as a result, it is important to design new and sophisticated methods that can aid in the detection of Hg2+. In this paper, two fluorescent dyes, carboxyfluorescein (FAM) and SYBR Green I, were used to label and intercalate DNA probes immobilized on the surface of graphene oxide (GO) for sensors to detect Hg2+. FAM and SYBR Green I dye share close excitation and emission wavelength spectra, which can promote and amplify the detection of signals, and also increase the limit of detection (LOD). The results showed that the limit of detection in this method was 0.53 nM. Moreover, when the sensors with double amino groups on the surface of GO were carried out to detect Hg2+, a limit of detection was improved to 0.43 nM. The sensors were then applied in the real sample. The results show that this method has a promising potential in Hg2+ detection.

scholarly journals Detection of Single Nucleotide Polymorphisms by Fluorescence Embedded Dye SYBR Green I Based on Graphene Oxide

2021 ◽  
Vol 9 ◽  
Author(s):  
Jiaoyun Xia ◽  
Tong Xu ◽  
Jing Qing ◽  
Lihua Wang ◽  
Junlong Tang
Keyword(s):  
Graphene Oxide ◽  
Single Nucleotide Polymorphisms ◽  
Fluorescence Emission ◽  
Sybr Green ◽  
Sybr Green I ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

The detection of single nucleotide polymorphisms (SNPs) is of great significance in the early diagnosis of diseases and the rational use of drugs. Thus, a novel biosensor based on the quenching effect of fluorescence-embedded SYBR Green I (SG) dye and graphene oxide (GO) was introduced in this study. The probe DNA forms a double helix structure with perfectly complementary DNA (pcDNA) and 15 single-base mismatch DNA (smDNA) respectively. SG is highly intercalated with perfectly complementary dsDNA (pc-dsDNA) and exhibits strong fluorescence emission. Single-base mismatch dsDNA (SNPs) has a loose double-stranded structure and exhibits poor SG intercalation and low fluorescence sensing. At this time, the sensor still showed poor SNP discrimination. GO has a strong effect on single-stranded DNA (ssDNA), which can reduce the fluorescence response of probe DNA and eliminate background interference. And competitively combined with ssDNA in SNPs, quenching the fluorescence of SG/SNP, while the fluorescence value of pc-dsDNA was retained, increasing the signal-to-noise ratio. At this time, the sensor has obtained excellent SNP resolution. Different SNPs detect different intensities of fluorescence in the near-infrared region to evaluate the sensor's identification of SNPs. The experimental parameters such as incubation time, incubation temperature and salt concentration were optimized. Under optimal conditions, 1 nM DNA with 0–10 nM linear range and differentiate 5% SNP were achieved. The detection method does not require labeling, is low cost, simple in operation, exhibits high SNP discrimination and can be distinguished by SNP at room temperature.

The influence of SYBR Green I on isothermal amplification with Bst exo DNA polymerase.

Biomics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 268-273
Author(s):  
A.R. Gilvanov ◽  
A.R. Sakhabutdinova ◽  
A.V. Chemeris ◽  
R.R. Garafutdinov
Keyword(s):  
Dna Polymerase ◽  
Sybr Green ◽  
Isothermal Amplification ◽  
Sybr Green I
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