Self-Spreading of Lipid Bilayer on a Hydrophobic Surface Made by Self-Assembled Monolayer with Short Alkyl Chain

2016 ◽  
Vol 16 (4) ◽  
pp. 3426-3430
Author(s):  
Yuya Omori ◽  
Hiroyuki Sakaue ◽  
Takayuki Takahagi ◽  
Hitoshi Suzuki
Keyword(s):  
Fluorescence Microscopy ◽  
Lipid Bilayer ◽  
Alkyl Chain ◽  
Hydrophobic Surface ◽  
Bilayer Membrane ◽  
The Self ◽  
Self Assembled Monolayer ◽  
Hydrophobic Region ◽  
Hydrophilic Region ◽  
Surface Modified

Behaviors of self-spreading of lipid bilayer membrane on a glass surface modified with selfassembled monolayer (SAM) with short alkyl chain were observed with fluorescence microscopy. Hydrophobic surface made by SAM was found to hamper the self-spreading phenomenon but the lipid bilayer spread on a hydrophilic one where SAM was decomposed by oxidation. On a binary surface having a hydrophobic region and a hydrophilic one, the lipid bilayer spread on the hydrophilic region but it stopped at the boundary of the hydrophobic region.

Study of Microdroplet Growth on Homogeneous and Patterned Surfaces Using Lattice Boltzmann Modeling

2019 ◽  
Vol 141 (6) ◽  
Author(s):  
Nilesh D. Pawar ◽  
Sunil R. Kale ◽  
Supreet Singh Bahga ◽  
Hassan Farhat ◽  
Sasidhar Kondaraju
Keyword(s):  
Contact Angle ◽  
Contact Line ◽  
Lattice Boltzmann ◽  
Growth Dynamics ◽  
Hydrophobic Surface ◽  
Droplet Growth ◽  
Patterned Surfaces ◽  
Hydrophobic Region ◽  
Hydrophilic Region ◽  
Contact Line Pinning

We present droplet growth dynamics on homogeneous and patterned surfaces (surface with hydrophilic and hydrophobic region) using two-dimensional thermal lattice Boltzmann method (LBM). In the first part, we performed 2D simulations on homogeneous hydrophobic surfaces. The result shows that the droplet grows at higher rate on a surface with higher wettability which is attributed to low conduction resistance and high solid–liquid contact area. In the later part, we performed simulations on patterned surface and observed that droplet preferentially nucleates on the hydrophilic region due to lower energy barrier and grows in constant contact line (CCL) mode because of contact line pinning at the interface of hydrophilic–hydrophobic region. As the contact angle reaches the maximum value of hydrophobic surface, contact line depins and droplet shows constant contact angle (CCA) growth mode. We also discuss the effect of characteristic width of hydrophilic region on growth of droplet. We show that contact angle of the droplet increases rapidly and reaches the contact angle of hydrophobic region on a surface with a lower width of the hydrophilic surface.

Supported lipid bilayer membrane arrays on micro-patterned ITO electrodes

RSC Advances ◽  
2016 ◽  
Vol 6 (76) ◽  
pp. 72821-72826 ◽  
Author(s):  
Xuejing Wang ◽  
Ying Zhang ◽  
Hongmei Bi ◽  
Xiaojun Han
Keyword(s):  
Cell Membrane ◽  
Fluorescence Microscopy ◽  
Lipid Bilayer ◽  
Electrochemical Detection ◽  
Bilayer Membrane ◽  
Lipid Bilayer Membrane ◽  
Biophysical Properties ◽  
Supported Lipid Bilayer ◽  
Array Platform ◽  
Lipid Bilayer Arrays

Lipid bilayer arrays were formed on micropatterned ITO electrodes. With this bilayer array platform both the fluorescence microscopy and electrochemical detection can be realized to explore the biophysical properties of cell membrane.

scholarly journals Transmembrane signal transduction by cofactor transport

2021 ◽  
Author(s):  
Istvan Kocsis ◽  
Yudi Ding ◽  
Nicholas H. Williams ◽  
Christopher A. Hunter
Keyword(s):  
Signal Transduction ◽  
Lipid Bilayer ◽  
Metal Ion ◽  
Bilayer Membrane ◽  
Ester Hydrolysis ◽  
Lipid Bilayer Membrane ◽  
Metal Ion Cofactors

Synthetic transducers transport externally added metal ion cofactors across the lipid bilayer membrane of vesicles to trigger catalysis of ester hydrolysis in the inner compartment. Signal transduction activity is modulated by hydrazone formation.

scholarly journals Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle

2020 ◽  
Vol 22 (1) ◽  
pp. 366
Author(s):  
Mao Arai ◽  
Tomohiro Miura ◽  
Yuriko Ito ◽  
Takatoshi Kinoshita ◽  
Masahiro Higuchi
Keyword(s):  
Binding Site ◽  
Lipid Bilayer ◽  
Structural Changes ◽  
Bilayer Membrane ◽  
Lipid Bilayer Membrane ◽  
Target Protein ◽  
Self Organization ◽  
Specific Response ◽  
Target Proteins ◽  
Recognition Ability

We designed and synthesized amphiphilic glycopeptides with glucose or galactose at the C-terminals. We observed the protein-induced structural changes of the amphiphilic glycopeptide assembly in the lipid bilayer membrane using transmission electron microscopy (TEM) and Fourier transform infrared reflection-absorption spectra (FTIR-RAS) measurements. The glycopeptides re-arranged to form a bundle that acted as an ion channel due to the interaction among the target protein and the terminal sugar groups of the glycopeptides. The bundle in the lipid bilayer membrane was fixed on a gold-deposited quartz crystal microbalance (QCM) electrode by the membrane fusion method. The protein-induced re-arrangement of the terminal sugar groups formed a binding site that acted as a receptor, and the re-binding of the target protein to the binding site induced the closing of the channel. We monitored the detection of target proteins by the changes of the electrochemical properties of the membrane. The response current of the membrane induced by the target protein recognition was expressed by an equivalent circuit consisting of resistors and capacitors when a triangular voltage was applied. We used peanut lectin (PNA) and concanavalin A (ConA) as target proteins. The sensing membrane induced by PNA shows the specific response to PNA, and the ConA-induced membrane responded selectively to ConA. Furthermore, PNA-induced sensing membranes showed relatively low recognition ability for lectin from Ricinus Agglutinin (RCA120) and mushroom lectin (ABA), which have galactose binding sites. The protein-induced self-organization formed the spatial arrangement of the sugar chains specific to the binding site of the target protein. These findings demonstrate the possibility of fabricating a sensing device with multi-recognition ability that can recognize proteins even if the structure is unknown, by the protein-induced self-organization process.

Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane

2008 ◽  
Vol 23 (6) ◽  
pp. 919-923 ◽  
Author(s):  
Martin Andersson ◽  
George Okeyo ◽  
Danyell Wilson ◽  
Henk Keizer ◽  
Paul Moe ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Bilayer ◽  
Bilayer Membrane ◽  
Lipid Bilayer Membrane

InGaZnO Thin-Film Transistors Modified by Self-Assembled Monolayer With Different Alkyl Chain Length

2015 ◽  
Vol 36 (7) ◽  
pp. 687-689 ◽  
Author(s):  
Peng Xiao ◽  
Linfeng Lan ◽  
Ting Dong ◽  
Zhenguo Lin ◽  
Sheng Sun ◽  
...  
Keyword(s):  
Thin Film ◽  
Chain Length ◽  
Thin Film Transistors ◽  
Alkyl Chain ◽  
Alkyl Chain Length ◽  
Self Assembled Monolayer ◽  
Self Assembled
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