Effectiveness of a Medaka Whole Testis Organ Culture System Using an Agarose Gel Stand for Germ Cell Proliferation and Differentiation

2019 ◽  
Vol 9 (2) ◽  
pp. 206-212
Author(s):  
Ji Hye Kang ◽  
Jun Hyung Ryu ◽  
Yoon Kwon Nam ◽  
Seung Pyo Gong
Keyword(s):  
Cell Proliferation ◽  
Organ Culture ◽  
Germ Cell ◽  
Culture System ◽  
Agarose Gel ◽  
Organ Culture System ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Germ Cell Proliferation

scholarly journals The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

Cell Research ◽  
2012 ◽  
Vol 22 (6) ◽  
pp. 1034-1045 ◽  
Author(s):  
Syed Irfan Ahmad Bukhari ◽  
Alejandro Vasquez-Rifo ◽  
Dominic Gagné ◽  
Eric R Paquet ◽  
Monique Zetka ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
C Elegans ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Microrna Pathway ◽  
Germ Cell Proliferation

scholarly journals Involvement of the D-Type Cyclins in Germ Cell Proliferation and Differentiation in the Mouse1

2000 ◽  
Vol 63 (6) ◽  
pp. 1893-1898 ◽  
Author(s):  
Tim L. Beumer ◽  
Hermien L. Roepers-Gajadien ◽  
Iris S. Gademan ◽  
Henk B. Kal ◽  
Dirk G. de Rooij
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Germ Cell Proliferation

Axial Stretch Increases Cell Proliferation in Arteries in Organ Culture

2000 ◽  
Author(s):  
Hai-Chao Han ◽  
Raymond P. Vito ◽  
Kristin Michael ◽  
David N. Ku
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Organ Culture ◽  
Vascular Function ◽  
Culture System ◽  
Carotid Arteries ◽  
Ex Vivo ◽  
Axial Stretch ◽  
Organ Culture System ◽  
Physiological Flow

Abstract To study the effect of axial stretch on vascular function and wall remodeling, porcine carotid arteries were cultured under conditions of physiological flow and elevated axial stretch in an ex vivo organ culture system. Smooth muscle cell proliferation was measured by bromodeoxyuridine index. Results showed that cell proliferation was significantly increased in the highly stretched arteries when compared to the normally stretched arteries. This may indicate the feasibility of stimulating new arterial growth by stretching natural arteries.

A coronary porcine organ culture system for studies of postangioplasty cell proliferation

1995 ◽  
Vol 6 (8) ◽  
pp. 657-666 ◽  
Author(s):  
Rainer Voisard ◽  
Verena Jensch ◽  
Regine Baur ◽  
Martin Höher ◽  
Vinzenz Hombach
Keyword(s):  
Cell Proliferation ◽  
Organ Culture ◽  
Culture System ◽  
Organ Culture System

scholarly journals A NOVEL ORGAN CULTURE SYSTEM SUPPORTED GERM CELL SURVIVAL IN PRIMATES AND SPERMATOGENESIS IN MICE

2020 ◽  
Vol 114 (3) ◽  
pp. e39
Author(s):  
Kien Tran ◽  
Wenbo Li ◽  
Sung Kwon Cho ◽  
Kyle E. Orwig
Keyword(s):  
Organ Culture ◽  
Germ Cell ◽  
Cell Survival ◽  
Culture System ◽  
Organ Culture System

P–017 The maintenance of testicular architecture and germ cell in adult testis tissue under organ culture condition based on the gas-liquid interface method

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M Komeya ◽  
H Odaka ◽  
T Matsumura ◽  
H Yamanaka ◽  
T Sato ◽  
...  
Keyword(s):  
Organ Culture ◽  
Germ Cell ◽  
Germ Cells ◽  
Culture Condition ◽  
Culture System ◽  
Liquid Interface ◽  
Adult Testis ◽  
In Vitro Spermatogenesis ◽  
Organ Culture System

Abstract Study question Can the gas-liquid interface organ culture system that achieved in vitro spermatogenesis in mice also support in vitro spermatogenesis in human adult testis? Summary answer Although the progression of spermatogenesis was not observed, germ cells were maintained without the degeneration of the architecture in both fresh and cryopreserved testicular tissues. What is known already Although the research on in vitro spermatogenesis have been conducted for 100 years, only the organ culture system using gas-liquid interface method achieved in vitro spermatogenesis in mice. It has not been verified whether this culture system can be applied to other mammals including humans and induce spermatogenesis. Study design, size, duration Testicular tissue was obtained from the transgender patients receiving sex reassignment surgery. Testicular specimens were either immediately processed for cultivation or cryopreserved, using a vitrification freezing protocol. Organ culture of testicular fragments was performed in three different media for a maximum period of 3 weeks to evaluate the short-term changes in the cultured tissues (viability, proliferation and maintenance of germ and somatic cells). Participants/materials, setting, methods Fresh and cryopreserved-thawed testis fragments (1–2 mm3) were cultured using the organ culture system in alpha-MEM with knock-out serum replacement (K group), alpha-MEM with lipid-rich BSA (A group) and DMEM with FBS (D group). Luteinizing hormone, follicle stimulating hormone and testosterone were supplemented. The number of germ cells (using DDX4), proliferative activity of germ cells (using EdU assay) and intratubular cell apoptosis (by TdT-mediated dUTP Nick End Labeling) were evaluated by immunohistochemical staining weekly. Main results and the role of chance The architecture of the seminiferous tubules was maintained until the second week of culture in both the fresh and the cryopreserved culture group. The number of DDX4-positive germ cells per seminiferous tubule in groups D, K, and A was 49 ± 24, 55 ± 21, 50 ± 26 cells/tubule in 1 day, 32 ± 13, 42 ± 7, 36 ± 21 cells/tubule in 1week, respectively. The numbers gradually decreased to 26 ± 8, 24 ± 6 and 27 ± 18 cells/tubule, in 2 weeks, respectively, with no difference among the groups. The number of intratubular EdU-positive cells of groups D, K, and A was 0.2 ± 0.2, 2.8 ± 2.1, 1.1 ± 0.8 cells/tubule at 1 day, 0.1 ± 0.2, 0.5 ± 0.6, 0.3 ± 0.6 cells/tubule at 1 week, respectively. The values were 0.01, 0.05, and 0.03 at 2 weeks. Thus, EdU-positive cells drastically decreased from the first week of culture. The number of DDX4-positive germ cells and the intratubular EdU-positive cells in the cryopreserved culture group was not different from that in the fresh culture group. Limitations, reasons for caution Current organ culture systems are incomplete, being unable to induce human in vitro spermatogenesis. Further research is needed to improve culture condition with the aim of producing fertile sperm of infertile adult male patients. Wider implications of the findings: Our organ culture system could maintain testis structure and germ cells. By using the testis tissues of the transgender patients, which are available with their consent, we will promote the investigation of the culture condition necessary for germ cell proliferation and differentiation. Trial registration number Grant-in-Aid for Scientific Research on Innovative Areas 18H05546, Grant-in-Aid for Young Scientists (A) 17H05098 and Takeda Science Foundation

scholarly journals Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription

2020 ◽  
Author(s):  
Sandeep Gopal ◽  
Aqilah Amran ◽  
Andre Elton ◽  
Leelee Ng ◽  
Roger Pocock
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Cell Fate ◽  
Notch Receptor ◽  
Cell Fate Decisions ◽  
Calcium Dependent ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Germline Proliferation ◽  
Membrane Bound

Notch receptors are essential membrane-bound regulators of cell proliferation and differentiation in metazoa. In the nematode Caenorhabditis elegans, correct expression of GLP-1 (germline proliferation-1), a germline-expressed Notch receptor, is important for germ cell maintenance. However, mechanisms that regulate GLP-1 expression are undefined. Here, we demonstrate that an AP-2 transcription factor (APTF-2) regulates GLP-1 expression through calcium-dependent binding to a conserved motif in the glp-1 promoter. Our data reveals that SDN-1 (syndecan-1), a transmembrane proteoglycan, regulates a TRP calcium channel in the soma to modulate the interaction between APTF-2 and glp-1 promoter - thus providing a potential communication nexus between the germline and its somatic environment to control germ cell fate decisions.

Sign in / Sign up

Export Citation Format

Share Document