scholarly journals The microRNA pathway controls germ cell proliferation and differentiation in C. elegans

Cell Research ◽  
2012 ◽  
Vol 22 (6) ◽  
pp. 1034-1045 ◽  
Author(s):  
Syed Irfan Ahmad Bukhari ◽  
Alejandro Vasquez-Rifo ◽  
Dominic Gagné ◽  
Eric R Paquet ◽  
Monique Zetka ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
C Elegans ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Microrna Pathway ◽  
Germ Cell Proliferation

scholarly journals Involvement of the D-Type Cyclins in Germ Cell Proliferation and Differentiation in the Mouse1

2000 ◽  
Vol 63 (6) ◽  
pp. 1893-1898 ◽  
Author(s):  
Tim L. Beumer ◽  
Hermien L. Roepers-Gajadien ◽  
Iris S. Gademan ◽  
Henk B. Kal ◽  
Dirk G. de Rooij
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Germ Cell Proliferation

scholarly journals mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans

Cell Research ◽  
2011 ◽  
Vol 21 (11) ◽  
pp. 1605-1618 ◽  
Author(s):  
Min Liu ◽  
Pengpeng Liu ◽  
Li Zhang ◽  
Qingchun Cai ◽  
Ge Gao ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
C Elegans ◽  
Intestine Cell ◽  
Germ Cell Proliferation

scholarly journals Mutations that Affect Channel Opening of Innexin Hemichannels in the C. elegans Gonad

2020 ◽  
Author(s):  
Todd Starich ◽  
David Greenstein
Keyword(s):  
Cell Proliferation ◽  
Gap Junctions ◽  
Germ Cell ◽  
Structural Model ◽  
Loss Of Function ◽  
C Elegans ◽  
Somatic Gonad ◽  
Channel Opening ◽  
Germline Proliferation ◽  
Germ Cell Proliferation

In C. elegans, gap junctions couple cells of the somatic gonad with the germline to support germ cell proliferation and gametogenesis. We previously characterized a strong loss-of-function mutation (T239I) affecting the second extracellular loop (EL2) of the somatic INX-8 hemichannel subunit. These mutant hemichannels form non-functional gap junctions with germline-expressed innexins. Here we describe the characterization of mutations that restore germ cell proliferation in the T239I EL2 mutant background. We recovered seven intragenic mutations located in diverse domains of INX-8 but not the EL domains. These second-site mutations compensate for the original channel defect to varying degrees, from nearly complete wild-type rescue, to partial rescue of germline proliferation. One suppressor mutation (E350K) supports the innexin cryo-EM structural model that the channel pore opening is surrounded by a cytoplasmic dome. Two suppressor mutations (S9L and I36N) may form leaky hemichannels that support germline proliferation but cause the demise of somatic sheath cells. Phenotypic analyses of three other suppressors reveal an equivalency in the rescue of germline proliferation and comparable delays in gametogenesis but a graded rescue of fertility. These latter mutations may be useful to probe interactions with the biochemical pathways that produce the molecules transiting through soma-germline gap junctions.

scholarly journals Notch-Directed Germ Cell Proliferation Is Mediated by Proteoglycan-Dependent Transcription

2020 ◽  
Author(s):  
Sandeep Gopal ◽  
Aqilah Amran ◽  
Andre Elton ◽  
Leelee Ng ◽  
Roger Pocock
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Cell Fate ◽  
Notch Receptor ◽  
Cell Fate Decisions ◽  
Calcium Dependent ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Germline Proliferation ◽  
Membrane Bound

Notch receptors are essential membrane-bound regulators of cell proliferation and differentiation in metazoa. In the nematode Caenorhabditis elegans, correct expression of GLP-1 (germline proliferation-1), a germline-expressed Notch receptor, is important for germ cell maintenance. However, mechanisms that regulate GLP-1 expression are undefined. Here, we demonstrate that an AP-2 transcription factor (APTF-2) regulates GLP-1 expression through calcium-dependent binding to a conserved motif in the glp-1 promoter. Our data reveals that SDN-1 (syndecan-1), a transmembrane proteoglycan, regulates a TRP calcium channel in the soma to modulate the interaction between APTF-2 and glp-1 promoter - thus providing a potential communication nexus between the germline and its somatic environment to control germ cell fate decisions.

scholarly journals PUF family proteins FBF-1 and FBF-2 regulate germline stem and progenitor cell proliferation and differentiation in C. elegans

2019 ◽  
Author(s):  
Xiaobo Wang ◽  
Mary Ellenbecker ◽  
Benjamin Hickey ◽  
Nicholas J. Day ◽  
Ekaterina Voronina
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Translational Control ◽  
Rna Binding ◽  
Germline Stem Cell ◽  
C Elegans ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Puf Family

ABSTRACTStem cells support tissue maintenance, but the mechanisms that balance the rate of stem cell self-renewal with differentiation at a population level remain uncharacterized. Through investigating the regulation of germline stem cells by two PUF family RNA-binding proteins FBF-1 and FBF-2 in C. elegans, we find that FBF-1 restricts differentiation, while FBF-2 promotes both proliferation and differentiation. FBFs act on a shared set of target mRNAs; however, FBF-1 destabilizes target transcripts, while FBF-2 promotes their accumulation. These regulatory differences result in complementary effects of FBFs on stem cells. We identify a mitotic cyclin as one of the targets affecting stem cell homeostasis. FBF-1-mediated translational control requires the activity of CCR4-NOT deadenylase. Distinct abilities of FBFs to cooperate with CCR4-NOT depend on protein sequences outside of the conserved PUF family RNA-binding domain. We propose that the combination of FBF activities regulates the dynamics of germline stem cell proliferation and differentiation.

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