Cranial Bone Defects Regeneration Using Platelet-Derived Growth Factor-BB-Loaded Chitosan/SBA-15 Composite Scaffold in a Rat Model

2016 ◽  
Vol 6 (7) ◽  
pp. 538-548 ◽  
Author(s):  
Dianqi Li ◽  
Xin Cheng ◽  
Qilong Wan ◽  
Qi Liu ◽  
Zubing Li
Keyword(s):  
Growth Factor ◽  
Rat Model ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Platelet Derived Growth Factor ◽  
Cranial Bone

scholarly journals Platelet-Derived Growth Factor Preserves Retinal Synapses in a Rat Model of Ocular Hypertension

2016 ◽  
Vol 57 (3) ◽  
pp. 842 ◽  
Author(s):  
Rachel S. Chong ◽  
Andrew Osborne ◽  
Raquel Conceição ◽  
Keith R. Martin
Keyword(s):  
Growth Factor ◽  
Ocular Hypertension ◽  
Rat Model ◽  
Platelet Derived Growth Factor

scholarly journals Platelet-derived growth factor B restores vascular barrier integrity and diminishes permeability in ovarian hyperstimulation syndrome

2020 ◽  
Vol 26 (8) ◽  
pp. 585-600
Author(s):  
Natalia Pascuali ◽  
Leopoldina Scotti ◽  
Gonzalo Oubiña ◽  
Ignacio de Zúñiga ◽  
Mariana Gomez Peña ◽  
...  
Keyword(s):  
At Risk ◽  
Growth Factor ◽  
Rat Model ◽  
Ovarian Hyperstimulation Syndrome ◽  
Regulatory Protein ◽  
Platelet Derived Growth Factor ◽  
Corpora Lutea ◽  
Ovarian Hyperstimulation ◽  
Serum Progesterone ◽  
Protein Levels

Abstract Although advances in the prediction and management of ovarian hyperstimulation syndrome (OHSS) have been introduced, complete prevention is not yet possible. Previously, we and other authors have shown that vascular endothelial growth factor, angiopoietins (ANGPTs) and sphingosine-1-phosphate are involved in OHSS etiology. In addition, we have demonstrated that ovarian protein levels of platelet-derived growth factor (PDGF) ligands -B and -D decrease in an OHSS rat model, whilst PDGFR-β and ANGPT2 remain unchanged. In the present work, we investigated the role of PDGF-B in OHSS by evaluating ligand protein levels in follicular fluid (FF) from women at risk of developing OHSS and by using an immature rat model of OHSS. We demonstrated that PDGF-B and PDGF-D are lower in FF from women at risk of developing OHSS compared to control patients (P < 0.05). In the OHSS rat model, PDGF-B (0.5 µg/ovary) administration decreased ovarian weight (P < 0.05), reduced serum progesterone (P < 0.05) and lowered the percentage of cysts (P < 0.05), compared to untreated OHSS rats, but had no effect on the proportion of follicles or corpora lutea (CL). PDGF-B treatment also restored the expression of steroidogenic acute regulatory protein (P < 0.05) and P450 cholesterol side-chain cleavage enzyme (P < 0.01) to control levels. In addition, PDGF-B increased the peri-endothelial cell area in CL and cystic structures, and reduced vascular permeability compared to untreated OHSS ovaries. Lastly, PDGF-B increased the levels of junction proteins claudin-5 (P < 0.05), occludin (P < 0.05) and β-catenin (P < 0.05), while boosting the extracellular deposition of collagen IV surrounding the ovarian vasculature (PP < 0.01), compared to OHSS alone. In conclusion, our findings indicate that PDGF-B could be another crucial mediator in the onset and development of OHSS, which may lead to the development of novel prediction markers and therapeutic strategies.

In vivoperformance of simvastatin-loaded electrospun spiral-wound polycaprolactone scaffolds in reconstruction of cranial bone defects in the rat model

2009 ◽  
Vol 90A (4) ◽  
pp. 1137-1151 ◽  
Author(s):  
Erhan Pişkin ◽  
İ. Alper İşoğlu ◽  
Nimet Bölgen ◽  
İbrahim Vargel ◽  
Sarah Griffiths ◽  
...  
Keyword(s):  
Rat Model ◽  
Bone Defects ◽  
Cranial Bone ◽  
Spiral Wound

scholarly journals Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

2021 ◽  
Vol 9 ◽  
Author(s):  
Youbin Li ◽  
Yuzhe Liu ◽  
Haotian Bai ◽  
Ronghang Li ◽  
Jing Shang ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Growth Factor ◽  
Composite Scaffold ◽  
Umbilical Vein ◽  
Tumor Resection ◽  
Bone Defects ◽  
Porous Titanium ◽  
Bone Integration

Tumor resection and treatment of trauma-related regional large bone defects have major challenges in the field of orthopedics. Scaffolds that treat bone defects are the focus of bone tissue engineering. 3D printing porous titanium alloy scaffolds, prepared via electron beam melting technology, possess customized structure and strength. The addition of a growth factor coating to the scaffold introduces a specific form of biological activation. Vascular endothelial growth factor (VEGF) is key to angiogenesis and osteogenesis in vivo. We designed a porous titanium alloy scaffold/thermosensitive collagen hydrogel system, equipped with VEGF, to promote local osseointegration and angiogenesis. We also verified the VEGF release via thermosensitive collagen and proliferation and induction of the human umbilical vein endothelial cells (HUVECs) via the composite system in vitro. In vivo, using microscopic computed tomography (Micro-CT), histology, and immunohistochemistry analysis, we confirmed that the composite scaffold aids in angiogenesis-mediated bone regeneration, and promotes significantly more bone integration. We also discovered that the composite scaffold has excellent biocompatibility, provides bioactive VEGF for angiogenesis and osteointegration, and provides an important theoretical basis for the restoration of local blood supply and strengthening of bone integration.

scholarly journals Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis

2021 ◽  
Vol 32 (12) ◽  
Author(s):  
Wanghan Xu ◽  
Wei Tan ◽  
Chan Li ◽  
Keke Wu ◽  
Xinyi Zeng ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Rat Model ◽  
Alveolar Bone ◽  
Composite Scaffold ◽  
Bone Defects ◽  
Promising Strategy ◽  
Good Biocompatibility ◽  
Positive Effect ◽  
Alveolar Bone Defect

AbstractPeriodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.

scholarly journals Epidermal growth factor or platelet-rich plasma combined with induced membrane technique in the treatment of segmental femur defects: an experimental study

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Ökkeş Bilal ◽  
Duran Topak ◽  
Mustafa Kınaş ◽  
Ergül Belge Kurutaş ◽  
Betül Kızıldağ ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Control Group ◽  
Therapeutic Benefits ◽  
Masquelet Technique ◽  
Membrane Technique ◽  
Epidermal Growth

Abstract Objective Extensive bone defects remain a therapeutic challenge necessitating alternative surgical approaches with better outcomes. Can increase the effectiveness of PRP or EGF treatment in surgical treatment of large bone defects with Masquelet technique? Aim of this study examined potential therapeutic benefits of the Masquelet technique with induced membranes in combination with platelet-rich plasma (PRP) or epidermal growth factor (EGF) in a rat model of segmental femur defect. Methods Three groups each consisting of 20 Sprague-Dawley rats were defined as follows: EGF group, PRP group, and control group. A femoral bone defect was created and filled with antibiotic embedded polymethyl methacrylate. Half of the animals in each group were sacrificed at week 6 and the pseudo-membranes formed were analyzed. In the remaining half, the cement was removed and the space was filled with autograft. After another 6 weeks, the structures formed were examined radiologically, histologically, and biochemically. Results At week 6, both PRP and EGF groups had significantly higher membrane CD31, TGF-beta, and VEGF levels than controls. At week 12, when compared to controls, PRP and EGF groups had significantly higher membrane CD31 levels and the PRP group had significantly higher membrane TGF levels. Regarding bone tissue levels, PRP and EGF groups had significantly higher VEGF levels and the EGF group had significantly higher BMP levels. In addition, PRP and EGF groups had higher radiological scores than controls. However, the two experimental groups did not differ with respect to any parameter tested in this study. Conclusion Both PRP and EGF seem to be associated with histological, biochemical, and radiological improvements in experimental rat model of Masquelet technique, warranting in further clinical studies. Level of evidence Level 5

scholarly journals Epidermal Growth Factor or Platelet Rich Plasma Combined With Induced Membrane Technique in the Treatment of Segmental Femur Defects: An Experimental Study

2020 ◽  
Author(s):  
Ökkeş Bilal ◽  
Duran Topak ◽  
Mustafa Kınaş ◽  
Ergül Belge Kurutaş ◽  
Betül Kızıldağ ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Control Group ◽  
Therapeutic Benefits ◽  
Masquelet Technique ◽  
Membrane Technique ◽  
Epidermal Growth

Abstract Objective: Extensive bone defects remain a therapeutic challenge necessitating alternative surgical approaches with better outcomes. Can increase the effectiveness of PRP or EGF treatment in surgical treatment of large bone defects with masquelet technique?Aim of this study examined potential therapeutic benefits of the Masquelet technique with induced membranes in combination with platelet rich plasma (PRP) or epidermal growth factor (EGF) in a rat model of segmental femur defect.Methods: Three groups each consisting of 20 Sprague-Dawley rats were defined as follows: EGF group, PRP group, and control group. A femoral bone defect was created and filled with antibiotic embedded polymethyl metacrylate. Half of the animals in each group were sacrificed at week 6 and the pseudo-membranes formed were analyzed. In the remaining half, the cement was removed and the space was filled with autograft. After another six weeks, the structures formed were examined radiologically, histologically and biochemically.Results: At week 6, both PRP and EGF groups had significantly higher membrane CD31, TGF and VEGF levels than controls. At week 12, when compared to controls, PRP and EGF groups had significantly higher membrane CD31 levels and PRP group had significantly higher membrane TGF levels. Regarding bone tissue levels, PRP and EGF groups had significantly higher VEGF levels and EGF group had significantly higher BMP levels. In addition, PRP and EGF groups had higher radiological scores than controls. However, the two experimental groups did not differ with respect to any parameter tested in this study. Conclusion: Both PRP and EGF seems to be associated with histological, biochemical, and radiological improvements in experimental rat model of Masquelet technique, warranting in further clinical studies.

scholarly journals Effect of recombinant human platelet-derived growth factor-BB-coated sutures on Achilles tendon healing in a rat model: A histological and biomechanical study

2012 ◽  
Vol 3 (1) ◽  
pp. 204173141245357 ◽  
Author(s):  
Stephen H Cummings ◽  
Daniel A Grande ◽  
Christopher K Hee ◽  
Hans K Kestler ◽  
Colleen M Roden ◽  
...  
Keyword(s):  
Growth Factor ◽  
Achilles Tendon ◽  
Rat Model ◽  
Human Platelet ◽  
Tendon Healing ◽  
Biomechanical Study ◽  
Platelet Derived Growth Factor
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