Polymer-Lipid Hybrid Theranostic Nanoparticles Co-Delivering Ultrasmall Superparamagnetic Iron Oxide and Paclitaxel for Targeted Magnetic Resonance Imaging and Therapy in Atherosclerotic Plaque

2016 ◽  
Vol 12 (6) ◽  
pp. 1245-1257 ◽  
Author(s):  
Yinmei Dong ◽  
Huaiwen Chen ◽  
Chao Chen ◽  
Xuefeng Zhang ◽  
Xia Tian ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Atherosclerotic Plaque ◽  
Superparamagnetic Iron Oxide ◽  
Resonance Imaging ◽  
Ultrasmall Superparamagnetic Iron Oxide ◽  
Theranostic Nanoparticles

scholarly journals Vascular permeability in the RG2 glioma model can be mediated by macropinocytosis and be independent of the opening of the tight junction

2016 ◽  
Vol 37 (4) ◽  
pp. 1264-1275
Author(s):  
Karin Pernet-Gallay ◽  
Pierre-Henri Jouneau ◽  
Anne Bertrand ◽  
Julie Delaroche ◽  
Régine Farion ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Electron Microscopy ◽  
Endothelial Cells ◽  
Iron Oxide ◽  
Magnetic Resonance ◽  
Immunoglobulin G ◽  
Superparamagnetic Iron Oxide ◽  
Resonance Imaging ◽  
Ultrasmall Superparamagnetic Iron Oxide ◽  
Glioma Model

This study evaluates the extravasation pathways of circulating macromolecules in a rat glioma model (RG2) which was observed by both magnetic resonance imaging using ultrasmall superparamagnetic iron oxide and electron microscopy. Although magnetic resonance imaging signal enhancement was observed as soon as 10 min after injection (9.4% 2 h after injection), electron microscopy showed that endothelial cells were still tightly sealed. However, circulating immunoglobulin G and ultrasmall superparamagnetic iron oxide were found in large membrane compartments of endothelial cells, in the basal lamina (7.4 ± 1.2 gold particles/µm2 in the tumor versus 0.38 ± 0.17 in healthy tissue, p = 1.4.10−5) and between tumoral cells. Altogether, this strongly suggests an active transport mediated by macropinocytosis. To challenge this transport mechanism, additional rats were treated with amiloride, an inhibitor of macropinocytosis, leading to a reduction of membrane protrusions (66%) and of macropinosomes. Amiloride however also opened tumoral tight junctions allowing a larger extravasation of ultrasmall superparamagnetic iron oxide (magnetic resonance imaging signal enhancement of 35.7% 2 h after injection). Altogether, these results suggest that ultrasmall superparamagnetic iron oxide and immunoglobulin G in the RG2 glioma model follow an active extravasation pathway mediated by a macropinocytosis process. Amiloride also appears as a potential strategy to facilitate the extravasation of chemotherapeutic drugs in glioma.

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