Simple and Biocompatible Ion Beam Micropatterning of a Cell-Repellent Polymer on Cell-Adhesive Surfaces to Manipulate Cell Adhesion

2016 ◽  
Vol 12 (2) ◽  
pp. 387-393 ◽  
Author(s):  
In-Tae Hwang ◽  
Chan-Hee Jung ◽  
Chang-Hee Jung ◽  
Jae-Hak Choi ◽  
Kwanwoo Shin ◽  
...  
Keyword(s):  
Ion Beam ◽  
2014 ◽  
Vol 59 (No. 10) ◽  
pp. 498-501 ◽  
Author(s):  
SY Heo ◽  
JW Seol ◽  
NS Kim

In this study, we fabricated Poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres using electrospinning and evaluated their potential use for bone repair applications. Analysis confirmed that the PLGA nanofibres were similar to the natural extracellular matrix and included HAp particles. Further, gelatin augmented the adhesion of electrospun nanofibres in the cell adhesion test. Therefore, electrospun PLGA/HAp nanofibres together with gelatin can be utilised for bone repair applications.  


2016 ◽  
Vol 4 (10) ◽  
pp. 1818-1826 ◽  
Author(s):  
D. S. Hernandez ◽  
E. T. Ritschdorff ◽  
S. K. Seidlits ◽  
C. E. Schmidt ◽  
J. B. Shear

A versatile and dynamic photoconjugation platform is introduced that provides high, 3D spatial resolution for functionalizing micro-3D-printed (μ-3DP) hydrogels. Schwann cells are patterned on μ-3DP hydrogels precisely labeled with RGD, a cell adhesive peptide, demonstrating utility of this platform for cell culture applications.


2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Dennis W. Zhou ◽  
Andrés J. García

Abstract Cell adhesion to the extracellular matrix (ECM) involves integrin receptor–ligand binding and clustering to form focal adhesion (FA) complexes, which mechanically link the cell’s cytoskeleton to the ECM and regulate fundamental cell signaling pathways. Although elucidation of the biochemical events in cell-matrix adhesive interactions is rapidly advancing, recent studies show that the forces underlying cell-matrix adhesive interactions are also critical to cell responses. Therefore, multiple measurement systems have been developed to quantify the spatial and temporal dynamics of cell adhesive forces, and these systems have identified how mechanical events influence cell phenotype and FA structure–function relationships under physiological and pathological settings. This review focuses on the development, methodology, and applications of measurement systems for probing (a) cell adhesion strength and (b) 2D and 3D cell traction forces.


2002 ◽  
Vol 81 (7) ◽  
pp. 497-500 ◽  
Author(s):  
A.M. Hoang ◽  
R.J. Klebe ◽  
B. Steffensen ◽  
O.H. Ryu ◽  
J.P. Simmer ◽  
...  

Amelogenin, the major protein component of tooth enamel, is shown to be a cell adhesion protein. Since it had been shown that an amelogenin-containing preparation, Emdogain®, possessed cell-adhesive activity, we tested the hypothesis that amelogenin was responsible for cell-adhesive activity. Recombinant amelogenin was found to promote adhesion at less than 15 μg/60-mm plate and requires divalent cations for activity. While we found that amelogenin does not bind to collagen or heparin under physiological conditions, it was demonstrated previously that amelogenin does bind to hydroxyapatite. The cell-adhesive activity of amelogenin may play a role in development and may provide a partial explanation for the therapeutic effects of Emdogain® in periodontal regeneration.


Author(s):  
D.M. Vanderwalker

There is a fundamental interest in electrochemical fusion of deuterium in palladium and titanium since its supposed discovery by Fleischmann and Pons. Their calorimetric experiments reveal that a large quantity of heat is released by Pd after hours in a cell, suggesting fusion occurs. They cannot explain fusion by force arguments, nor can it be an exothermic reaction on the formation of deuterides because a smaller quantity of heat is released. This study examines reactions of deuterium in titanium.Both iodide titanium and 99% pure titanium samples were encapsulated in vacuum tubes, annealed for 2h at 800 °C. The Ti foils were charged with deuterium in a D2SO4 D2O solution at a potential of .45V with respect to a calomel reference junction. Samples were ion beam thinned for transmission electron microscopy. The TEM was performed on the JEOL 200CX.The structure of D charged titanium is α-Ti with hexagonal and fee deuterides.


2011 ◽  
Vol 29 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Qi-Lian Liang ◽  
Guo-Qiang Chen ◽  
Zhou-Yu Li ◽  
Bi-Rong Wang

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