Functionalizing micro-3D-printed protein hydrogels for cell adhesion and patterning

2016 ◽  
Vol 4 (10) ◽  
pp. 1818-1826 ◽  
Author(s):  
D. S. Hernandez ◽  
E. T. Ritschdorff ◽  
S. K. Seidlits ◽  
C. E. Schmidt ◽  
J. B. Shear
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Schwann Cells ◽  
Spatial Resolution ◽  
Cell Adhesive ◽  
A Cell ◽  
3D Printed ◽  
Protein Hydrogels

A versatile and dynamic photoconjugation platform is introduced that provides high, 3D spatial resolution for functionalizing micro-3D-printed (μ-3DP) hydrogels. Schwann cells are patterned on μ-3DP hydrogels precisely labeled with RGD, a cell adhesive peptide, demonstrating utility of this platform for cell culture applications.

scholarly journals Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 171-177
Author(s):  
Nobuyuki Tanaka ◽  
Ryoji Sekine ◽  
Shun-ichi Funano ◽  
Asako Sato ◽  
Núria Taberner Carretero ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Polymer Film ◽  
Cell Patterning ◽  
Ethanol Solution ◽  
Rapid Fabrication ◽  
A Cell ◽  
Culture Surface

This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.

Development of a flexible 3D printed scaffold with a cell-adhesive surface for artificial trachea

2019 ◽  
Vol 14 (5) ◽  
pp. 055001 ◽  
Author(s):  
Chi Bum Ahn ◽  
Kuk Hui Son ◽  
Young Soo Yu ◽  
Tae Ho Kim ◽  
Jae Ik Lee ◽  
...  
Keyword(s):  
Adhesive Surface ◽  
Cell Adhesive ◽  
A Cell ◽  
3D Printed

scholarly journals Characterisation and assessment of electrospun Poly/hydroxyapatite nanofibres together with a cell adhesive for bone repair applications

2014 ◽  
Vol 59 (No. 10) ◽  
pp. 498-501 ◽  
Author(s):  
SY Heo ◽  
JW Seol ◽  
NS Kim
Keyword(s):  
Extracellular Matrix ◽  
Cell Adhesion ◽  
Bone Repair ◽  
Adhesion Test ◽  
Cell Adhesive ◽  
A Cell ◽  
Electrospun Nanofibres ◽  
Potential Use

In this study, we fabricated Poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HAp) nanofibres using electrospinning and evaluated their potential use for bone repair applications. Analysis confirmed that the PLGA nanofibres were similar to the natural extracellular matrix and included HAp particles. Further, gelatin augmented the adhesion of electrospun nanofibres in the cell adhesion test. Therefore, electrospun PLGA/HAp nanofibres together with gelatin can be utilised for bone repair applications.  

3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture

2012 ◽  
Vol 28 (4) ◽  
pp. 1055-1060 ◽  
Author(s):  
Wan-Geun La ◽  
Suk Ho Bhang ◽  
Jung-Youn Shin ◽  
Hee Hun Yoon ◽  
Jooyeon Park ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Free Cell ◽  
Serum Free ◽  
A Cell

scholarly journals Folic acid modified clay/polymer nanocomposites for selective cell adhesion

2014 ◽  
Vol 2 (37) ◽  
pp. 6412-6421 ◽  
Author(s):  
F. B. Barlas ◽  
D. Ag Seleci ◽  
M. Ozkan ◽  
B. Demir ◽  
M. Seleci ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Folic Acid ◽  
Polymer Nanocomposites ◽  
Promising Material ◽  
Modified Clay ◽  
A Cell ◽  
Cell Adhesion And Proliferation

A promising material, a folic acid modified poly(epsilon-caprolactone)/clay nanocomposite that allows selective cell adhesion and proliferation, was synthesized and characterized as a cell culture and biosensing platform.

Going beyond RGD: screening of a cell-adhesion peptide library in 3D cell culture

2020 ◽  
Vol 15 (5) ◽  
pp. 055033
Author(s):  
Mariah Sarwat ◽  
Denver C Surrao ◽  
Nick Huettner ◽  
James A St John ◽  
Tim R Dargaville ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
3D Cell Culture ◽  
Peptide Library ◽  
A Cell

Purification of Hexabrachion (Tenascin) from Cell Culture Conditioned Medium, and Separation from a Cell Adhesion Factor

Matrix ◽  
1990 ◽  
Vol 10 (2) ◽  
pp. 98-111 ◽  
Author(s):  
I. Aukhil ◽  
C.C. Slemp ◽  
V.A. Lightner ◽  
K. Nishimura ◽  
G. Briscoe ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Conditioned Medium ◽  
A Cell ◽  
Adhesion Factor

scholarly journals Measurement Systems for Cell Adhesive Forces

2015 ◽  
Vol 137 (2) ◽  
Author(s):  
Dennis W. Zhou ◽  
Andrés J. García
Keyword(s):  
Cell Adhesion ◽  
Temporal Dynamics ◽  
Cell Phenotype ◽  
Measurement Systems ◽  
Cell Matrix ◽  
Cell Adhesive ◽  
Cell Traction ◽  
A Cell ◽  
Adhesive Interactions ◽  
Adhesive Forces

Abstract Cell adhesion to the extracellular matrix (ECM) involves integrin receptor–ligand binding and clustering to form focal adhesion (FA) complexes, which mechanically link the cell’s cytoskeleton to the ECM and regulate fundamental cell signaling pathways. Although elucidation of the biochemical events in cell-matrix adhesive interactions is rapidly advancing, recent studies show that the forces underlying cell-matrix adhesive interactions are also critical to cell responses. Therefore, multiple measurement systems have been developed to quantify the spatial and temporal dynamics of cell adhesive forces, and these systems have identified how mechanical events influence cell phenotype and FA structure–function relationships under physiological and pathological settings. This review focuses on the development, methodology, and applications of measurement systems for probing (a) cell adhesion strength and (b) 2D and 3D cell traction forces.

Simple and Biocompatible Ion Beam Micropatterning of a Cell-Repellent Polymer on Cell-Adhesive Surfaces to Manipulate Cell Adhesion

2016 ◽  
Vol 12 (2) ◽  
pp. 387-393 ◽  
Author(s):  
In-Tae Hwang ◽  
Chan-Hee Jung ◽  
Chang-Hee Jung ◽  
Jae-Hak Choi ◽  
Kwanwoo Shin ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Ion Beam ◽  
Cell Adhesive ◽  
A Cell

Amelogenin is a Cell Adhesion Protein

2002 ◽  
Vol 81 (7) ◽  
pp. 497-500 ◽  
Author(s):  
A.M. Hoang ◽  
R.J. Klebe ◽  
B. Steffensen ◽  
O.H. Ryu ◽  
J.P. Simmer ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Tooth Enamel ◽  
Periodontal Regeneration ◽  
Major Protein ◽  
Therapeutic Effects ◽  
Partial Explanation ◽  
Adhesion Protein ◽  
Cell Adhesion Protein ◽  
Cell Adhesive ◽  
A Cell

Amelogenin, the major protein component of tooth enamel, is shown to be a cell adhesion protein. Since it had been shown that an amelogenin-containing preparation, Emdogain®, possessed cell-adhesive activity, we tested the hypothesis that amelogenin was responsible for cell-adhesive activity. Recombinant amelogenin was found to promote adhesion at less than 15 μg/60-mm plate and requires divalent cations for activity. While we found that amelogenin does not bind to collagen or heparin under physiological conditions, it was demonstrated previously that amelogenin does bind to hydroxyapatite. The cell-adhesive activity of amelogenin may play a role in development and may provide a partial explanation for the therapeutic effects of Emdogain® in periodontal regeneration.

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