Polymeric Surface-Mediated, High-Density Nano-Assembly of Functional Protein Arrays

2011 ◽  
Vol 7 (6) ◽  
pp. 731-742 ◽  
Author(s):  
Jong-In Hahm
Keyword(s):  
High Density ◽  
Functional Protein ◽  
Protein Arrays ◽  
Polymeric Surface

scholarly journals Next-generation high-density self-assembling functional protein arrays

2008 ◽  
Vol 5 (6) ◽  
pp. 535-538 ◽  
Author(s):  
Niroshan Ramachandran ◽  
Jacob V Raphael ◽  
Eugenie Hainsworth ◽  
Gokhan Demirkan ◽  
Manuel G Fuentes ◽  
...  
Keyword(s):  
High Density ◽  
Next Generation ◽  
Functional Protein ◽  
Protein Arrays ◽  
Self Assembling

Self-assembly of vesicle nanoarrays on Si: A potential route to high-density functional protein arrays

2007 ◽  
Vol 90 (3) ◽  
pp. 033901 ◽  
Author(s):  
C. S. Ramanujan ◽  
K. Sumitomo ◽  
M. R. R. de Planque ◽  
H. Hibino ◽  
K. Torimitsu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Density Functional ◽  
High Density ◽  
Functional Protein ◽  
Protein Arrays

scholarly journals Designed and biologically active protein lattices

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shih-Ting Wang ◽  
Brian Minevich ◽  
Jianfang Liu ◽  
Honghu Zhang ◽  
Dmytro Nykypanchuk ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Biologically Active ◽  
Double Layers ◽  
Active Protein ◽  
Functional Protein ◽  
Protein Arrays ◽  
Iron Storage ◽  
Nanoscale Systems ◽  
Framework Design ◽  
2D And 3D

AbstractVersatile methods to organize proteins in space are required to enable complex biomaterials, engineered biomolecular scaffolds, cell-free biology, and hybrid nanoscale systems. Here, we demonstrate how the tailored encapsulation of proteins in DNA-based voxels can be combined with programmable assembly that directs these voxels into biologically functional protein arrays with prescribed and ordered two-dimensional (2D) and three-dimensional (3D) organizations. We apply the presented concept to ferritin, an iron storage protein, and its iron-free analog, apoferritin, in order to form single-layers, double-layers, as well as several types of 3D protein lattices. Our study demonstrates that internal voxel design and inter-voxel encoding can be effectively employed to create protein lattices with designed organization, as confirmed by in situ X-ray scattering and cryo-electron microscopy 3D imaging. The assembled protein arrays maintain structural stability and biological activity in environments relevant for protein functionality. The framework design of the arrays then allows small molecules to access the ferritins and their iron cores and convert them into apoferritin arrays through the release of iron ions. The presented study introduces a platform approach for creating bio-active protein-containing ordered nanomaterials with desired 2D and 3D organizations.

scholarly journals Protein kinase substrate identification on functional protein arrays

2008 ◽  
Vol 8 (1) ◽  
pp. 22 ◽  
Author(s):  
Lihao Meng ◽  
Gregory A Michaud ◽  
Janie S Merkel ◽  
Fang Zhou ◽  
Jing Huang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Functional Protein ◽  
Protein Arrays ◽  
Kinase Substrate ◽  
Substrate Identification

NANOSTRUCTURED DENDRIMER MODIFIED GLASS SURFACES FOR PROTEIN MICROARRAY

2007 ◽  
Vol 06 (02) ◽  
pp. 161-165 ◽  
Author(s):  
PARAYIL KUMARAN AJIKUMAR ◽  
JIN KIAT NG ◽  
JIM YANG LEE ◽  
GREGORY STEPHANOPOULOS ◽  
HENG-PHON TOO
Keyword(s):  
Glass Surface ◽  
Density Functional ◽  
Protein Microarray ◽  
Pamam Dendrimer ◽  
High Density ◽  
Protein Arrays ◽  
Nonspecific Adsorption ◽  
Cell Lysate ◽  
Modified Surface ◽  
Glass Surfaces

Carboxyl PAMAM dendrimer (3.5 generation) was covalently coupled onto amine modified glass surface to prepare a monolayer of high density functional linkers. Activation of the carboxyl PAMAM surface and the subsequent immobilization of antibodies resulted in a high density protein microarray as compared to linear carboxyl linker surface. In addition, fluorescent labeled cell lysate showed extremely low nonspecific adsorption to the PAMAM modified surface, comparable to inert PEG surface. Thus, the carboxyl PAMAM modified surface is ideal for the generation of high density protein arrays for the detection of dilute antigens with superior sensitivity.

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