Self-assembly of vesicle nanoarrays on Si: A potential route to high-density functional protein arrays

2007 ◽  
Vol 90 (3) ◽  
pp. 033901 ◽  
Author(s):  
C. S. Ramanujan ◽  
K. Sumitomo ◽  
M. R. R. de Planque ◽  
H. Hibino ◽  
K. Torimitsu ◽  
...  
Keyword(s):  
Self Assembly ◽  
Density Functional ◽  
High Density ◽  
Functional Protein ◽  
Protein Arrays

scholarly journals Next-generation high-density self-assembling functional protein arrays

2008 ◽  
Vol 5 (6) ◽  
pp. 535-538 ◽  
Author(s):  
Niroshan Ramachandran ◽  
Jacob V Raphael ◽  
Eugenie Hainsworth ◽  
Gokhan Demirkan ◽  
Manuel G Fuentes ◽  
...  
Keyword(s):  
High Density ◽  
Next Generation ◽  
Functional Protein ◽  
Protein Arrays ◽  
Self Assembling

NANOSTRUCTURED DENDRIMER MODIFIED GLASS SURFACES FOR PROTEIN MICROARRAY

2007 ◽  
Vol 06 (02) ◽  
pp. 161-165 ◽  
Author(s):  
PARAYIL KUMARAN AJIKUMAR ◽  
JIN KIAT NG ◽  
JIM YANG LEE ◽  
GREGORY STEPHANOPOULOS ◽  
HENG-PHON TOO
Keyword(s):  
Glass Surface ◽  
Density Functional ◽  
Protein Microarray ◽  
Pamam Dendrimer ◽  
High Density ◽  
Protein Arrays ◽  
Nonspecific Adsorption ◽  
Cell Lysate ◽  
Modified Surface ◽  
Glass Surfaces

Carboxyl PAMAM dendrimer (3.5 generation) was covalently coupled onto amine modified glass surface to prepare a monolayer of high density functional linkers. Activation of the carboxyl PAMAM surface and the subsequent immobilization of antibodies resulted in a high density protein microarray as compared to linear carboxyl linker surface. In addition, fluorescent labeled cell lysate showed extremely low nonspecific adsorption to the PAMAM modified surface, comparable to inert PEG surface. Thus, the carboxyl PAMAM modified surface is ideal for the generation of high density protein arrays for the detection of dilute antigens with superior sensitivity.

scholarly journals A S‐Layer Protein of Bacillus anthracis as a Building Block for Functional Protein Arrays by In Vitro Self‐Assembly

Small ◽  
2015 ◽  
Vol 11 (43) ◽  
pp. 5826-5832 ◽  
Author(s):  
Xu‐Ying Wang ◽  
Dian‐Bing Wang ◽  
Zhi‐Ping Zhang ◽  
Li‐Jun Bi ◽  
Ji‐Bin Zhang ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Self Assembly ◽  
Building Block ◽  
Functional Protein ◽  
Protein Arrays

scholarly journals Self-assembly of N-heterocyclic carbenes on Au(111)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Alex Inayeh ◽  
Ryan R. K. Groome ◽  
Ishwar Singh ◽  
Alex J. Veinot ◽  
Felipe Crasto de Lima ◽  
...  
Keyword(s):  
Self Assembly ◽  
Density Functional ◽  
Surface Coverage ◽  
High Mobility ◽  
Heterocyclic Carbenes ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Surface Geometry ◽  
Functional Theory ◽  
Interesting Alternative

AbstractAlthough the self-assembly of organic ligands on gold has been dominated by sulfur-based ligands for decades, a new ligand class, N-heterocyclic carbenes (NHCs), has appeared as an interesting alternative. However, fundamental questions surrounding self-assembly of this new ligand remain unanswered. Herein, we describe the effect of NHC structure, surface coverage, and substrate temperature on mobility, thermal stability, NHC surface geometry, and self-assembly. Analysis of NHC adsorption and self-assembly by scanning tunneling microscopy and density functional theory have revealed the importance of NHC-surface interactions and attractive NHC-NHC interactions on NHC monolayer structures. A remarkable way these interactions manifest is the need for a threshold NHC surface coverage to produce upright, adatom-mediated adsorption motifs with low surface diffusion. NHC wingtip structure is also critical, with primary substituents leading to the formation of flat-lying NHC2Au complexes, which have high mobility when isolated, but self-assemble into stable ordered lattices at higher surface concentrations. These and other studies of NHC surface chemistry will be crucial for the success of these next-generation monolayers.

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