Comparative Genomics of Pseudomonas aeruginosa PAO1 and Pseudomonas putida KT2440: Orthologs, Codon Usage, Repetitive Extragenic Palindromic Elements, and Oligonucleotide Motif Signatures

2002 ◽  
Vol 1 (4) ◽  
pp. 175-187 ◽  
Author(s):  
Christian Weinel ◽  
David W. Ussery ◽  
Hakan Ohlsson ◽  
Thomas Sicheritz-Ponten ◽  
Claudia Kiewitz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Comparative Genomics ◽  
Codon Usage ◽  
Pseudomonas Putida ◽  
Repetitive Extragenic Palindromic

Biodegradation of chlorophenols by immobilized pure-culture microorganisms

1996 ◽  
Vol 34 (10) ◽  
pp. 67-72 ◽  
Author(s):  
Lu Chih-Jen ◽  
Lee Chi-Mei ◽  
Huang Chiou-Zong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Pure Culture ◽  
The Other ◽  
Batch Reactors ◽  
Agrobacterium Radiobacter ◽  
Culture Cells ◽  
Lag Period

The biodegradation of phenol and chlorophenols by immobilized pure-culture cells was conducted by a series of batch reactors. The microorganisms used in this study were Pseudomonas putida, Psuedomonas testosteroni, Pseudomonas aeruginosa, and Agrobacterium radiobacter. All four species showed the ortho-cleavage pathway to metabolize chlorophenols. Among the four species, P. testosteroni, P. putida, and P. aeruginosa could effectively remove phenol at 200 mg/l. P. testosteroni could effectively remove 2-chlorophenol at 10mg/l. However, the other three species, P. putida, P. aeruginosa, and A. radiobacter, could not effectively remove 2-chlorophenol. Although 3-chlorophenol is a recalcitrant compound, P. testosteroni also could rapidly metabolize 3-chlorophenol at 10 mg/l. The removal of 4-chlorophenol at 10 mg/l by P. testosteroni reached 98% within one day. P. aeruginosa and A. radiobacter also could metabolize 4-chlorophenol after 2 and 7 days of lag period, respectively.

scholarly journals High‐throughput dilution‐based growth method enables time‐resolved exo‐metabolomics of Pseudomonas putida and Pseudomonas aeruginosa

2021 ◽  
Author(s):  
Bjarke H. Pedersen ◽  
Nicolás Gurdo ◽  
Helle Krogh Johansen ◽  
Søren Molin ◽  
Pablo I. Nikel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
High Throughput ◽  
Time Resolved ◽  
Growth Method

scholarly journals Conjugal TOL Transfer from Pseudomonas putida to Pseudomonas aeruginosa: Effects of Restriction Proficiency, Toxicant Exposure, Cell Density Ratios, and Conjugation Detection Method on Observed Transfer Efficiencies

2005 ◽  
Vol 71 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Catalina Arango Pinedo ◽  
Barth F. Smets
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Strong Dependence ◽  
Plasmid Transfer ◽  
Mass Action ◽  
Tol Plasmid ◽  
Toxicant Exposure ◽  
Transfer Frequency ◽  
Cell Densities

ABSTRACT The effects of restriction proficiency and premating exposure to toxicants on conjugal transfer of the TOL plasmid between Pseudomonas spp. was investigated by examinations of filter matings. A Pseudomonas putida KT2442-derived strain carrying a gfp-tagged variant of the TOL plasmid was used as a donor, and both restriction-deficient (PAO1162N) and -proficient (PAO2002N) Pseudomonas aeruginosa strains were used as recipients. The in situ enumeration of conjugation events allowed us to obtain frequency estimates that were unbiased by transconjugant growth or plasmid retransfer. We observed a strong dependence of the plasmid transfer frequency on the initial donor-to-recipient ratio of surface matings, which invalidated the use of mass action-based plasmid transfer kinetic estimators. Careful control of the initial parental cell densities permitted evaluations of the true effects of restriction proficiency and toxicant exposure on TOL transfer. At standard donor-to-recipient ratios (10−3 for PAO1162N and 2 � 101 for PAO2002N) and total cell densities (105 cells/mm2 for PAO1162N and 106 cells/mm2 for PAO2002N), plasmid transfer frequencies without toxicant exposure were approximately 10−7 (events/mm2)−1 for PAO1162N and 10−11 (events/mm2)−1 for PAO2002N based on in situ observations of conjugation events. The enumeration of transconjugants via selective plating yielded transfer frequencies that were up to 1 order of magnitude lower. Premating exposure to sodium dodecyl sulfate (1 to 10 mM) significantly increased the transfer frequency for the restriction-proficient strain PAO2002N (P < 0.05) but not for the restriction-deficient strain PAO1162N. On the other hand, premating exposure to ethanol, toluene, or phenol had no positive effect on the plasmid transfer frequency. Clearly, restriction proficiency provides a strong barrier to interspecific transfer of the TOL plasmid, and this barrier was only marginally attenuated by recipient exposure to toxicants within the ranges examined.

scholarly journals Comparative genomics of clinical strains of Pseudomonas aeruginosa strains isolated from different geographic sites

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Dinesh Subedi ◽  
Ajay Kumar Vijay ◽  
Gurjeet Singh Kohli ◽  
Scott A. Rice ◽  
Mark Willcox
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Comparative Genomics ◽  
Clinical Strains

scholarly journals Comparative genomics of an endophytic Pseudomonas putida isolated from mango orchard

2016 ◽  
Vol 39 (3) ◽  
pp. 465-473 ◽  
Author(s):  
Huma Asif ◽  
David J. Studholme ◽  
Asifullah Khan ◽  
M. Aurongzeb ◽  
Ishtiaq A. Khan ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Pseudomonas Putida

scholarly journals nalD Encodes a Second Repressor of the mexAB-oprM Multidrug Efflux Operon of Pseudomonas aeruginosa

2006 ◽  
Vol 188 (24) ◽  
pp. 8649-8654 ◽  
Author(s):  
Yuji Morita ◽  
Lily Cao ◽  
Virginia C. Gould ◽  
Matthew B. Avison ◽  
Keith Poole
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Stenotrophomonas Maltophilia ◽  
Multidrug Efflux ◽  
Efflux System

ABSTRACT The Pseudomonas aeruginosa nalD gene encodes a TetR family repressor with homology to the SmeT and TtgR repressors of the smeDEF and ttgABC multidrug efflux systems of Stenotrophomonas maltophilia and Pseudomonas putida, respectively. A sequence upstream of mexAB-oprM and overlapping a second promoter for this efflux system was very similar to the SmeT and TtgR operator sequences, and NalD binding to this region was, in fact, demonstrated. Moreover, increased expression from this promoter was seen in a nalD mutant, consistent with NalD directly controlling mexAB-oprM expression from a second promoter.

scholarly journals WS01-2 Comparative genomics study of a set of Pseudomonas aeruginosa isolates from the TORPEDO-CF trial

2019 ◽  
Vol 18 ◽  
pp. S1 ◽  
Author(s):  
A. Cazares ◽  
W. Figueroa ◽  
D. Kenna ◽  
S. Langton-Hewer ◽  
A. Smyth ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Comparative Genomics
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