High Sensitivity Electrochemical Immunosensor Based on Plasma Modified TiO2/Chitosan for Ochratoxin-A Detection

2013 ◽  
Vol 1 (2) ◽  
pp. 148-152 ◽  
Author(s):  
Raju Khan ◽  
K. K. Saini ◽  
Marshal Dhayal
Keyword(s):  
Ochratoxin A ◽  
High Sensitivity ◽  
Electrochemical Immunosensor

scholarly journals Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2738
Author(s):  
Xue Zhao ◽  
Xindi Jin ◽  
Zhang Lin ◽  
Qi Guo ◽  
Bin Liu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
High Performance ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Strong Stability ◽  
Test Strip ◽  
Chromatography Analysis ◽  
Relative Standard ◽  
Strip Test

Spices are susceptible to contamination by aflatoxin B1 (AFB1) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB1 and OTA in spices by spraying the coupled antigens AFB1–ovalbumin (AFB1–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB1 and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB1 and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.

scholarly journals Dramatically Enhancing the Sensitivity of Immunoassay for Ochratoxin A Detection by Cascade-Amplifying Enzyme Loading

Toxins ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 781
Author(s):  
Zhuolin Song ◽  
Lin Feng ◽  
Yuankui Leng ◽  
Mingzhu Huang ◽  
Hao Fang ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Cross Reaction ◽  
Molar Ratio ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enzyme Loading ◽  
M13 Bacteriophage ◽  
Mimic Peptide

Enzyme-linked immunosorbent assay (ELISA) is widely used in the routine screening of mycotoxin contamination in various agricultural and food products. Herein, a cascade-amplifying system was introduced to dramatically promote the sensitivity of an immunoassay for ochratoxin A (OTA) detection. Specifically, a biotinylated M13 bacteriophage was introduced as a biofunctional competing antigen, in which a seven-peptide OTA mimotope fused on the p3 protein of M13 was used to specifically recognize an anti-OTA monoclonal antibody, and the biotin molecules modified on capsid p8 proteins were used in loading numerous streptavidin-labeled polymeric horseradish peroxidases (HRPs). Owing to the abundance of biotinylated p8 proteins in M13 and the high molar ratio between HRP and streptavidin in streptavidin-polyHRP, the loading amount of HRP enzymes on the M13 bacteriophage were greatly boosted. Hence, the proposed method exhibited high sensitivity, with a limit of detection of 2.0 pg/mL for OTA detection, which was 250-fold lower than that of conventional ELISA. In addition, the proposed method showed a slight cross-reaction of 2.3% to OTB, a negligible cross-reaction for other common mycotoxins, and an acceptable accuracy for OTA quantitative detection in real corn samples. The practicability of the method was further confirmed with a traditional HRP-based ELISA method. In conclusion, the biotinylated bacteriophage and polyHRP structure showed potential as a cascade-amplifying enzyme loading system for ultra-trace OTA detemination, and its application can be extended to the detection of other analytes by altering specific mimic peptide sequences.

Electrochemical immunosensor for ochratoxin A detection based on Au octahedron plasmonic colloidosomes

2018 ◽  
Vol 1032 ◽  
pp. 114-121 ◽  
Author(s):  
Tong Zhang ◽  
Bin Xing ◽  
Qingzhi Han ◽  
Yunfeng Lei ◽  
Dan Wu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Immunosensor

Application of Gold Nanoparticles for High-Sensitivity Fluorescence Polarization Aptamer Assay for Ochratoxin A

2019 ◽  
Vol 14 (7-8) ◽  
pp. 397-404 ◽  
Author(s):  
A. V. Samokhvalov ◽  
I. V. Safenkova ◽  
S. A. Eremin ◽  
A. V. Zherdev ◽  
B. B. Dzantiev
Keyword(s):  
Gold Nanoparticles ◽  
Ochratoxin A ◽  
Fluorescence Polarization ◽  
High Sensitivity ◽  
Aptamer Assay

An Ultrasensitive Immunosensor for the Detection of Carcinoembryonic Antigens Utilizing a Nb-Doped Titanium Dioxide Nanocomposite Film

NANO ◽  
2015 ◽  
Vol 10 (04) ◽  
pp. 1550060 ◽  
Author(s):  
He Li ◽  
Junli Lu ◽  
Yu Wang ◽  
Fenyun Yi ◽  
Xindong Guo
Keyword(s):  
Titanium Dioxide ◽  
Influence Factors ◽  
High Sensitivity ◽  
Electrochemical Immunosensor ◽  
Optimal Conditions ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Electrochemical Signal ◽  
Doped Titanium Dioxide ◽  
Excellent Selectivity

An ultrasensitive electrochemical immunosensor based on Nb-doped titanium dioxide was developed for detection of carcinoembryonic antigen (CEA). The electrochemical signal enhancement was due to the synergistic effect by NbT and nanogold. The influence factors (pH, temperature and incubation time) were investigated in detail. Under optimal conditions, the immunosensor was able to detect the concentration of CEA in the range of 0.5 ng ⋅ mL-1 to 150 ng ⋅ mL-1, with a lower detection limit of 8.95 pg ⋅ mL-1. The proposed immunosensor showed high sensitivity, excellent selectivity and good stability.

Fluorescence aptasensor for Ochratoxin A in food samples based on hyperbranched rolling circle amplification

2015 ◽  
Vol 7 (15) ◽  
pp. 6109-6113 ◽  
Author(s):  
Ying Zhang ◽  
Linlin Yang ◽  
Cuiying Lin ◽  
Longhua Guo ◽  
Bin Qiu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Food Samples ◽  
Rolling Circle

A fluorescence aptasensor for Ochratoxin A based on hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

An electrochemical immunosensor for ochratoxin A based on immobilization of antibodies on diazonium-functionalized gold electrode

2009 ◽  
Vol 54 (8) ◽  
pp. 2180-2184 ◽  
Author(s):  
Abd-Elgawad Radi ◽  
Xavier Muñoz-Berbel ◽  
Montserrat Cortina-Puig ◽  
Jean-Louis Marty
Keyword(s):  
Ochratoxin A ◽  
Gold Electrode ◽  
Electrochemical Immunosensor

A self-assembled electrochemical immunosensor for ultra-sensitive detection of ochratoxin A in medicinal and edible malt

2020 ◽  
Vol 315 ◽  
pp. 126289 ◽  
Author(s):  
Chaonan Sun ◽  
Xiaofang Liao ◽  
Pinxuan Huang ◽  
Guangzhi Shan ◽  
Xiao Ma ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Self Assembled

scholarly journals Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 71 ◽  
Author(s):  
Mina Nan ◽  
Yang Bi ◽  
Huali Xue ◽  
Sulin Xue ◽  
Haitao Long ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Self Assembly ◽  
Electrochemical Impedance ◽  
Rapid Determination ◽  
Grape Juice ◽  
High Sensitivity ◽  
Layer By Layer ◽  
Label Free ◽  
Transfer Resistance

A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.

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