scholarly journals Glycosylated Hemoglobin A1 Predicts Risk for Symptomatic Hemorrhage After Thrombolysis for Acute Stroke

Stroke ◽  
2013 ◽  
Vol 44 (8) ◽  
pp. 2134-2138 ◽  
Author(s):  
A. Rocco ◽  
P. U. Heuschmann ◽  
P. D. Schellinger ◽  
M. Kohrmann ◽  
J. Diedler ◽  
...  
Keyword(s):  
Acute Stroke ◽  
Glycosylated Hemoglobin ◽  
Hemoglobin A1

New spectrophotometric method for the determination of hemoglobin A1 compared with a microcolumn technique.

1982 ◽  
Vol 28 (1) ◽  
pp. 96-99 ◽  
Author(s):  
O Wålinder ◽  
G Ronquist ◽  
P J Fager
Keyword(s):  
Phytic Acid ◽  
Spectrophotometric Method ◽  
Healthy Subjects ◽  
Large Scale ◽  
Glycosylated Hemoglobin ◽  
Standard Curve ◽  
Sugar Moiety ◽  
Hemoglobin A1 ◽  
The Mean

Abstract We compared a spectrophotometric kit method (Glycospec) for determination of glycosylated hemoglobin (HbA1) with a microcolumn kit method (Bio-Rad). The Glycospec method is based on the change in absorbance when phytic acid binds to hemoglobin A. With glycosylated hemoglobin there is no such change because the binding is blocked by the sugar moiety. Inter-assay CVs were 2-6% for both methods. In healthy subjects the mean (+/- SD) value for HbAl was about 1% higher with the spectrophotometric than the microcolumn method. For samples from 122 diabetics the correlation between values for HbAl obtained with the two methods was acceptable (r = 0.89), although the spectrophotometric technique yielded 2-4% higher values, a difference at least partly due to the absence of 2,3-diphosphoglycerate from the spectrophotometric standards. Adding 1.8 mmol of it per liter to these standards caused displacement of the standard curve; HbAl values then agreed well with those of the microcolumn method. The spectrophotometric procedure is easily automated, and therefore is well suited for large-scale analyses if problems with standards and calibration can be solved.

scholarly journals Glycosylated hemoglobin A2 components

Blood ◽  
1980 ◽  
Vol 56 (3) ◽  
pp. 571-572 ◽  
Author(s):  
C Tegos ◽  
E Beutler
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Glycosylated Hemoglobin ◽  
Diabetic Patients ◽  
Agar Gel ◽  
Hemoglobin A ◽  
Hemoglobin A1 ◽  
Agar Gel Electrophoresis ◽  
Derivatives Of ◽  
Hemoglobin A2

Abstract Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glycohemoglobins was obtained by showing that 14C-glucose bound to hemoglobin A2 moved with these minor bands. The amounts of glycohemoglobin derivatives of hemoglobin A2 were increased in the blood of diabetic patients.

Glycosylated hemoglobin A1 in a random group of adult onset diabetics of indian subpopulation

1992 ◽  
Vol 7 (2) ◽  
pp. 138-142
Author(s):  
Tangirala Malati ◽  
D. Murali Mohan Krishna ◽  
V. R. Srinivasan ◽  
V. Shantharam
Keyword(s):  
Glycosylated Hemoglobin ◽  
Adult Onset ◽  
Random Group ◽  
Hemoglobin A1

scholarly journals Glycosylated hemoglobin A2 components

Blood ◽  
1980 ◽  
Vol 56 (3) ◽  
pp. 571-572
Author(s):  
C Tegos ◽  
E Beutler
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Glycosylated Hemoglobin ◽  
Diabetic Patients ◽  
Agar Gel ◽  
Hemoglobin A ◽  
Hemoglobin A1 ◽  
Agar Gel Electrophoresis ◽  
Derivatives Of ◽  
Hemoglobin A2

Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glycohemoglobins was obtained by showing that 14C-glucose bound to hemoglobin A2 moved with these minor bands. The amounts of glycohemoglobin derivatives of hemoglobin A2 were increased in the blood of diabetic patients.

Labile glycosylated hemoglobin contributes to hemoglobin A1 as measured by liquid chromatography or electrophoresis.

1981 ◽  
Vol 27 (7) ◽  
pp. 1261-1263 ◽  
Author(s):  
D M Nathan
Keyword(s):  
Liquid Chromatography ◽  
Blood Glucose Concentration ◽  
Glycosylated Hemoglobin ◽  
Isotonic Saline ◽  
High Pressure Liquid Chromatographic ◽  
Normal Cells ◽  
Labile Fraction ◽  
Hemoglobin A1 ◽  
Acute Changes ◽  
Liquid Chromatographic

Abstract The utility of glycosylated hemoglobin (HbA1) measurement as an index of chronic control in diabetes can be adversely affected by interference from a labile glycosylated fraction that changes rapidly with acute changes in blood glucose concentration. I used a "high-pressure" liquid-chromatographic assay and a newly developed electrophoretic assay to quantitate the contribution of this labile fraction. If erythrocytes are incubated at 22 degrees C in isotonic saline for 12 h before hemolysis, the labile fraction is eliminated. Its contribution is similar as measured by both assays: 2-3.5% of total HbA1 in normal cells and 7-9.5% in diabetic cells. A 3-h incubation of erythrocytes with glucose produces acute changes in apparent HbA1 concentrations by both assays, but such changes can be eliminated by the incubation in saline. Although current methods are time consuming, the labile glycosylated hemoglobin must be removed when the sample is prepared for HbA1 measurement by liquid chromatography and electrophoresis if results are not to be factitiously high.

Rapid method for eliminating labile glycosylated hemoglobin from the assay for hemoglobin A1.

1982 ◽  
Vol 28 (3) ◽  
pp. 512-515 ◽  
Author(s):  
D M Nathan ◽  
E Avezzano ◽  
J L Palmer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Blood Glucose ◽  
High Performance ◽  
Chemical Method ◽  
Glycosylated Hemoglobin ◽  
Acidic Ph ◽  
Blood Samples ◽  
Hemoglobin A1 ◽  
Whole Blood Samples

Abstract The irreversible formation of stable glycosylated hemoglobin proceeds through a labile intermediate that is indistinguishable, by most methods, from the stable glycosylated product. The inclusion of the labile intermediate, which changes acutely with acute blood glucose changes, detracts from the utility of the assay as an index of chronic glucose concentration. We have developed a rapid, reliable chemical method for eliminating the labile intermediate: a 30-min incubation of whole-blood samples with semi-carbazide (30 mmol/L) and aniline (12 mmol/L) at pH 5 and 38 degrees C. The semicarbazide serves as a glucose trap; the transfer of glucose from the labile glycosylated hemoglobin to the semicarbazide is catalyzed by the acidic pH and aniline. This treatment is effective in the three most commonly used assays: "high-performance" liquid chromatography, electrophoresis, and a minicolumn kit.

scholarly journals Stress hyperglycemia and electrolytes disturbance in patients with acute cerebrovascular stroke

2019 ◽  
Vol 55 (1) ◽  
Author(s):  
Bastawy M. El-Fawal ◽  
Reda Badry ◽  
Wael A. Abbas ◽  
Ahmed K. Ibrahim
Keyword(s):  
Acute Stroke ◽  
Heart Diseases ◽  
Glycosylated Hemoglobin ◽  
Morbidity And Mortality ◽  
Medical Emergency ◽  
Mortality Data ◽  
Stroke Severity ◽  
High Morbidity ◽  
Stress Hyperglycemia ◽  
Electrolyte Disturbance

Abstract Background Stroke is a medical emergency and considered the 2nd leading cause of mortality worldwide. High morbidity and mortality of stroke are due to some complications like cerebral edema, infection, and associated heart disease. Stroke may cause stress hyperglycemia and electrolyte disturbance that may be independent predictors for increased morbidity and mortality. Data on electrolyte disorder in neurological conditions especially acute stroke are somewhat scanty and not frequently interpreted. Objectives The current study aimed to evaluate the effect of stress induced by acute stroke on the blood glucose and serum electrolytes and its relationship to the type, severity, and prognosis of stroke. Subjects and methods A total of 100 patients admitted with acute stroke were included in the study. After informed consent, fasting blood sugar, glycosylated hemoglobin, serum sodium, and potassium levels were measured simultaneously. Stroke severity and outcome were assessed using the Glasgow Coma Scale. Results Stress hyperglycemia was noted in 21 patients, out of which 62% presented with hemorrhagic stroke. Undiagnosed diabetes was found in 47% of cases. Also, 13 % of our patients had both stress hyperglycemia and electrolyte disturbance simultaneously. Mortality among hemorrhagic versus ischemic patients was 46% and 22%, respectively. The independent predictors of mortality were stress hyperglycemia, electrolytes disturbance, ischemic heart diseases, and type and admission severity of stroke. Conclusion Stress hyperglycemia and electrolyte disturbance after acute stroke were found in 21% and 47% of patients respectively and may adversely affect outcome of stroke especially those with cerebral hemorrhage.

Quantitiative determination of glycosylated hemoglobin A1 by agar gel electrophoresis.

1980 ◽  
Vol 26 (11) ◽  
pp. 1598-1602 ◽  
Author(s):  
L Menard ◽  
M E Dempsey ◽  
L A Blankstein ◽  
H Aleyassine ◽  
M Wacks ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Glycosylated Hemoglobin ◽  
Agar Gel ◽  
Hemoglobin A1 ◽  
Agar Gel Electrophoresis
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