Topical Applications of a Heparinoid-Containing Product Attenuate Glucocorticoid-Induced Alterations in Epidermal Permeability Barrier in Mice

2021 ◽  
Vol 34 (2) ◽  
pp. 86-93
Author(s):  
Si Wen ◽  
Jiangmei Wu ◽  
Li Ye ◽  
Bin Yang ◽  
Lizhi Hu ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Skin Surface ◽  
Clobetasol Propionate ◽  
Permeability Barrier ◽  
Surface Ph ◽  
Expression Levels ◽  
Stratum Corneum Hydration ◽  
Epidermal Permeability Barrier ◽  
Skin Surface Ph ◽  
Topical Applications

Introduction: Either systemic or topical glucocorticoids (GCs) can cause significant adverse effects on cutaneous structure and function. Although some products and ingredients can improve GC-induced abnormalities in epidermal permeability barrier, the efficacy is moderate. Prior studies in normal mice showed that topical applications of a heparinoid-containing product, Hirudoid® cream, improve epidermal barrier function by upregulation of epidermal proliferation, expression of mRNA for epidermal differentiation, and lipid production. Objective: The objective of this study was to assess whether topical applications of this product could prevent GC-induced changes in epidermal function in murine skin. Materials and Methods: One group of C57BL/6J mice was treated topically with 0.05% clobetasol propionate twice daily for 6 days, while another group was treated topically with Hirudoid® cream 30 min after each application of clobetasol propionate. Untreated mice served as normal controls. Transepidermal water loss (TEWL) rates, stratum corneum hydration, and skin surface pH were measured using respective probes connected to an MPA5 physiology monitor. qPCR was used to measure the expression levels of mRNA for keratinocyte differentiation-related proteins and lipid synthetic enzymes. Results: Co-applications of Hirudoid® cream with GC minimally, but significantly, increased skin thickness in comparison to GC treatment alone (p < 0.05), in parallel with increased expression levels of mRNA for PCNA in both the dermis and the epidermis. Moreover, Hirudoid® cream largely prevented GC-induced elevation in basal TEWL (p < 0.001) and delay in barrier recovery (p < 0.05), accompanied by upregulation in the expression levels of mRNA for epidermal involucrin, HMGCoA, and SPT1. However, both stratum corneum hydration and skin surface pH were comparable in the skin treated with GC alone versus GC + Hirudoid® cream. Conclusion: Topical heparinoid-containing product can partially prevent GC-induced alterations in some epidermal functions.

scholarly journals Stratum Corneum Hydration and Skin Surface pH Variation Indicate that Organ Blood Flow Is Regulated by Meridian Activity at Certain Hours

2014 ◽  
Vol 2 (4) ◽  
pp. 161-172
Author(s):  
Li-Fan Chuang ◽  
Hong-Nong Chou ◽  
Chin-Kong Hsu ◽  
Hung-Shih Chou ◽  
Ping-Jyun Sung ◽  
...  
Keyword(s):  
Blood Flow ◽  
Stratum Corneum ◽  
Skin Surface ◽  
Organ Blood Flow ◽  
Surface Ph ◽  
Ph Variation ◽  
Stratum Corneum Hydration ◽  
Skin Surface Ph

scholarly journals Topical Applications of a Novel Emollient Inhibit Inflammation in Murine Models of Acute Contact Dermatitis

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Si Wen ◽  
Mengke Sun ◽  
Li Ye ◽  
Bin Yang ◽  
Lizhi Hu ◽  
...  
Keyword(s):  
Contact Dermatitis ◽  
Stratum Corneum ◽  
Permeability Barrier ◽  
Murine Models ◽  
Inflammatory Infiltration ◽  
Cutaneous Inflammation ◽  
Expression Levels ◽  
Stratum Corneum Hydration ◽  
Eczematous Dermatitis ◽  
Topical Applications

The benefits of emollients for eczematous dermatitis and psoriasis have been thought to be due to the improvements in epidermal function, including epidermal permeability barrier, stratum corneum hydration, and stratum corneum pH. We determined here whether emollient can direct inhibit cutaneous inflammation. Ear inflammation was induced by topical application of either 12-O-tetradecanoylphorbol-13-acetate (TPA) or 1-fluoro-2,4-dinitrofluorobenzene (DNFB). Either 1% hydrocortisone cream or the novel emollient was applied to the right ear of the mice 45 min and 2 hours after TPA or DNFB application. The untreated left ear served as untreated controls. Both ear weight and ear thickness were measured 24 hours after TPA and DNFB application. Topical applications of either hydrocortisone cream or emollient significantly decreased both ear thickness and ear weight in comparison to untreated controls. In DNFB model, hydrocortisone significantly lowered expression levels of mRNA for IL-1α, IL-1β, and TNFα, while the emollient markedly decreased expression levels of IL-1α and TNFα mRNA. In TPA model, both hydrocortisone and emollient significantly decreased expression levels of IL-1α, IL-1β, IL-6, and TNFα mRNA. In parallel, inflammatory infiltration was also reduced by topical applications of either hydrocortisone or emollient. These results demonstrate that this novel emollient can directly inhibit cutaneous inflammation in murine models of both acute irritant contact dermatitis and acute allergic contact dermatitis. However, whether this emollient could also alleviate eczematous dermatitis in humans remains to be explored.

scholarly journals Heavy Cigarette Smokers in a Chinese Population Display a Compromised Permeability Barrier

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Shujun Xin ◽  
Li Ye ◽  
George Man ◽  
Chengzhi Lv ◽  
Peter M. Elias ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Skin Surface ◽  
Transepidermal Water Loss ◽  
Permeability Barrier ◽  
Biophysical Parameters ◽  
Cigarette Smokers ◽  
Recovery Rates ◽  
Surface Ph ◽  
Epidermal Permeability Barrier ◽  
Skin Surface Ph

Cigarette smoking is associated with various cutaneous disorders with defective permeability. Yet, whether cigarette smoking influences epidermal permeability barrier function is largely unknown. Here, we measured skin biophysical properties, including permeability barrier homeostasis, stratum corneum (SC) integrity, SC hydration, skin surface pH, and skin melanin/erythema index, in cigarette smokers. A total of 99 male volunteers were enrolled in this study. Smokers were categorized as light-to-moderate (<20 cigarettes/day) or heavy smokers (≥20 cigarettes/day). An MPA5 was used to measure SC hydration and skin melanin/erythema index on the dorsal hand, forehead, and cheek. Basal transepidermal water loss (TEWL) and barrier recovery rates were assessed on the forearm. A Skin-pH-Meter pH900 was used to measure skin surface pH. Our results showed that heavy cigarette smokers exhibited delayed barrier recovery after acute abrogation (1.02%±13.06versus16.48%±6.07), and barrier recovery rates correlated negatively with the number of daily cigarettes consumption (p=0.0087). Changes in biophysical parameters in cigarette smokers varied with body sites. In conclusion, heavy cigarette smokers display compromised permeability barrier homeostasis, which could contribute, in part, to the increased prevalence of certain cutaneous disorders characterized by defective permeability. Thus, improving epidermal permeability barrier should be considered for heavy cigarette smokers.

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