scholarly journals Interleukin-8 Levels in the Stratum Corneum as a Biomarker for Monitoring Therapeutic Effect in Atopic Dermatitis Patients

2021 ◽  
pp. 1-15
Author(s):  
Susumu Murata ◽  
Sakae Kaneko ◽  
Eishin Morita
Keyword(s):  
Atopic Dermatitis ◽  
Topical Corticosteroid ◽  
Stratum Corneum ◽  
Corticosteroid Treatment ◽  
Skin Inflammation ◽  
Interleukin 8 ◽  
Tape Stripping ◽  
Therapeutic Effects ◽  
Local Skin ◽  
Before And After

<b><i>Introduction:</i></b> The stratum corneum contains several growth factors and cytokines that are synthesized in keratinocytes. We previously reported that the amount of interleukin-8 in the stratum corneum (scIL-8) is related to the severity of local skin inflammation in atopic dermatitis (AD). However, it is unknown whether scIL-8 levels reflect pharmacologic responses to a therapeutic intervention in AD patients. Therefore, in this study, we aimed to investigate whether the improvement of dermatitis in AD is correlated with scIL-8 levels before and after topical corticosteroid treatment. <b><i>Methods:</i></b> Stratum corneum samples were collected from 22 AD patients using the noninvasive tape-stripping method before treatment, 2 weeks after topical treatment, and 4–6 weeks after treatment. <b><i>Results:</i></b> scIL-8 levels on the forearm reduced significantly from 790 ± 348 pg/mg before treatment to 163 ± 68 pg/mg 2 weeks after treatment and 100 ± 37 pg/mg 4–6 weeks after corticosteroid treatment. scIL-8 levels on the abdomen also reduced significantly from 902 ± 391 to 142 ± 38 pg/mg at the end of study. The reduction in scIL-8 levels was associated with the improvement in local skin severity in AD. We also found that scIL-8 levels, along with blood biomarker levels (serum thymus and activation-regulated chemokine, lactate dehydrogenase, and %eosinophil), decreased significantly after the treatment. <b><i>Conclusion:</i></b> The scIL-8 concentration decreases with improvements in skin symptoms in AD patients after topical corticosteroid treatment; thus, it may be a suitable biomarker for monitoring therapeutic effects in AD patients.

scholarly journals Stratum Corneum Sampling to Assess Bioequivalence between Topical Acyclovir Products

2019 ◽  
Vol 36 (12) ◽  
Author(s):  
A. Pensado ◽  
W.S. Chiu ◽  
S. F. Cordery ◽  
E. Rantou ◽  
A. L. Bunge ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Statistical Power ◽  
Application Site ◽  
Tape Stripping ◽  
Local Skin ◽  
Drug Products ◽  
Single Time Point ◽  
Topical Acyclovir ◽  
Alternative Metrics

Abstract Purpose To examine the potential of stratum corneum (SC) sampling via tape-stripping in humans to assess bioequivalence of topical acyclovir drug products, and to explore the potential value of alternative metrics of local skin bioavailability calculable from SC sampling experiments. Methods Three acyclovir creams were considered in two separate studies in which drug amounts in the SC after uptake and clearance periods were measured and used to assess bioequivalence. In each study, a “reference” formulation (evaluated twice) was compared to the “test” in 10 subjects. Each application site was replicated to achieve greater statistical power with fewer volunteers. Results SC sampling revealed similarities and differences between products consistent with results from other surrogate bioequivalence measures, including dermal open-flow microperfusion experiments. Further analysis of the tape-stripping data permitted acyclovir flux into the viable skin to be deduced and drug concentration in that ‘compartment’ to be estimated. Conclusions Acyclovir quantities determined in the SC, following a single-time point uptake and clearance protocol, can be judiciously used both to objectively compare product performance in vivo and to assess delivery of the active into skin tissue below the barrier, thereby permitting local concentrations at or near to the site of action to be determined.

Antimicrobial Silk Clothing in the Treatment of Atopic Dermatitis Proves Comparable to Topical Corticosteroid Treatment

Dermatology ◽  
2006 ◽  
Vol 213 (3) ◽  
pp. 228-233 ◽  
Author(s):  
G. Senti ◽  
L.S. Steinmann ◽  
B. Fischer ◽  
R. Kurmann ◽  
T. Storni ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Topical Corticosteroid ◽  
Corticosteroid Treatment

Stratum corneum TARC level is a new indicator of lesional skin inflammation in atopic dermatitis

Allergy ◽  
2010 ◽  
Author(s):  
E. Morita ◽  
H. Takahashi ◽  
H. Niihara ◽  
I. Dekio ◽  
Y. Sumikawa ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Skin Inflammation ◽  
Lesional Skin

scholarly journals Side-by-Side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis

2019 ◽  
Vol 139 (11) ◽  
pp. 2387-2389.e1 ◽  
Author(s):  
Byung Eui Kim ◽  
Elena Goleva ◽  
Peter S. Kim ◽  
Kathryn Norquest ◽  
Caroline Bronchick ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Skin Biopsies

scholarly journals Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

2016 ◽  
Vol 170 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Sjors A. Koppes ◽  
Richard Brans ◽  
Suzana Ljubojevic Hadzavdic ◽  
Monique H.W. Frings-Dresen ◽  
Thomas Rustemeyer ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Inflammatory Mediators ◽  
Topical Therapy ◽  
Tape Stripping

Interleukin-8 Content in the Stratum Corneum as an Indicator of the Severity of Inflammation in the Lesions of Atopic Dermatitis

2013 ◽  
Vol 160 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Tseveendorj Amarbayasgalan ◽  
Hitoshi Takahashi ◽  
Itaru Dekio ◽  
Eishin Morita
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Interleukin 8

scholarly journals Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Maja-Lisa Clausen ◽  
H.-C. Slotved ◽  
Karen A. Krogfelt ◽  
Tove Agner
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Healthy Skin

scholarly journals Bacterially Delivered miRNA-Mediated Toll-like Receptor 8 Gene Silencing for Combined Therapy in a Murine Model of Atopic Dermatitis: Therapeutic Effect of miRTLR8 in AD

2021 ◽  
Vol 9 (8) ◽  
pp. 1715
Author(s):  
Wonsuck Yoon ◽  
Eun-Jae Kim ◽  
Yongsung Park ◽  
Seunghyun Kim ◽  
Yong-Keun Park ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Gene Silencing ◽  
Combined Therapy ◽  
Adaptive Immune Response ◽  
Skin Inflammation ◽  
Interleukin 4 ◽  
Therapeutic Effects ◽  
Skin Reactions ◽  
Adaptive Immune

In atopic dermatitis (AD), skin inflammation is caused by complex interactions between genetic disposition and aberrant innate/adaptive immune responses. Toll-like receptors (TLRs) are key molecules in the innate/adaptive immune response as they recognize various molecular motifs associated with pathogens. Among them, TLR8 is implicated in eczematous skin reactions. We investigated the combined therapeutic effects of TLR8 gene silencing by the bacterial delivery of miRNA. We used Salmonella as a vector to deliver TLR8 miRNA. The recombinant strain of Salmonella enterica subsp. enterica serovar Typhimurium (ST) expressing TLR8 miRNA (ST-miRTLR8) was prepared for knockdown of TLR8. After oral administration of ST-miRTLR8 into mice, we observed the cytokine levels, skin pathology and scratching behaviors in an AD-like mouse model. TLR8 down-regulation decreased macrophage-derived chemokine concentrations in activated human mast cells. Serum IgE and interleukin-4 production were suppressed whereas IFN-γ was induced after oral administration of ST-miRTLR8. Scratching behaviors and skin inflammation were also improved. In addition, attenuated S. typhimurium safely accumulated in mouse macrophages and showed adjuvant effects. This study shows that the recombinant miRNA that expresses the TLR8 miRNA has therapeutic effects by suppressing Th2 inflammation. TLR gene modulation using miRNA via Salmonella vectors will thus have a double-protective effect in the treatment of AD.

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