scholarly journals Evaluation of the in vitro Function of Platelet Concentrates from Pooled Buffy Coats or Apheresis

2020 ◽  
Vol 47 (4) ◽  
pp. 314-325
Author(s):  
Sarah Anna Fiedler ◽  
Klaus Boller ◽  
Ann-Christine Junker ◽  
Christel Kamp ◽  
Anneliese Hilger ◽  
...  
Keyword(s):  
Shear Stress ◽  
Annexin V ◽  
Storage Conditions ◽  
Platelet Concentrate ◽  
Platelet Concentrates ◽  
Stability Parameters ◽  
Single Donor ◽  
Transmission Electron ◽  
Soluble Cd40l

Background: Platelet concentrates play an important role in transfusion medicine. Their short lifespan and lack of robustness require efforts to ensure adequate product quality. In this study, we compared the in vitro quality of the main concentrate types, pooled platelet concentrate (PPC) from whole blood donations, and platelet concentrate from single-donor apheresis (APC). Methods: Twenty PPCs and 20 APCs prepared in plasma were analyzed on days 2, 4, and 7 of storage. Variables related to metabolism, degranulation, platelet aggregation, P-selectin expression, and annexin V binding were analyzed. Morphology was assessed by transmission electron microscopy of ultrathin sections. A microfluidic device was applied to test the effects of shear stress on platelet function. Results: The metabolic parameters indicated stable storage conditions throughout the 7-day period. The resting discoid form was the prevailing morphology on days 2 and 4 in the PPCs and APCs. Chemokine release and receptor shedding of soluble P-selectin and soluble CD40L equally increased in PPCs and APCs. Aggregation responses to ADP and collagen were heterogeneous, with marked losses in collagen responsiveness on day 4 in individual concentrates. Baseline expression of P-selectin in PPCs and APCs was low, and inducibility of P-selectin was well preserved until day 4. Under shear stress, equal adhesiveness and stability were found with platelets from PPCs and APCs. Conclusions: Platelets from PPCs and APCs showed similar in vitro function and stability parameters. However, platelet concentrates presented a high variability and individual concentrates an impaired functional capability. Identifying the factors contributing to this would help increase product reliability.

Soluble CD40L and CD62P levels differ in single‐donor apheresis platelet concentrates and buffy coat–derived pooled platelet concentrates

Transfusion ◽  
2018 ◽  
Vol 59 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Caroline Sut ◽  
Sofiane Tariket ◽  
Chaker Aloui ◽  
Charles‐Antoine Arthaud ◽  
Marie‐Ange Eyraud ◽  
...  
Keyword(s):  
Buffy Coat ◽  
Platelet Concentrates ◽  
Single Donor ◽  
Soluble Cd40l

CONTAMINATING LEUKOCYTES INFLUENCE THE STORAGE CONDITIONS OF PLATELET CONCENTRATES

1987 ◽  
Author(s):  
R N I Pietersz ◽  
D de Korte ◽  
D Roos ◽  
H W Reesink
Keyword(s):  
Ph Value ◽  
Hla Antigens ◽  
Lactate Production ◽  
Critical Factor ◽  
Glucose Consumption ◽  
Storage Conditions ◽  
Rank Test ◽  
Platelet Concentrates ◽  
Group I

Leukocyte poor platelet concentrates (PC), containing less than 10 leukocytes, prepared from buffycoats can be stored in normal PVC bags for 7 days at 22°C without deterioration of the pH. We assumed that a low number of leukocytes present in the PC, is a critical factor to maintain the pH. To test this hypothesis increasing amounts of leukocytes were added to four groups of three PC with comparable plasma volumes (mean 58.6 ± 0.8 (SD) ml) and platelet concentrations (1.01 ± 0.04×109 /ml). Group I had a leukocyte concentration of 0.14±0.048×106 /ml, group II 1.96±0.09×106 /ml, group III 5.53±0.98×106 /ml, and group IV 13.0±0.93×106 /ml. The PC were stored in normal PVC bags for 7 days at 22°C. Measurements in vitro were performed at day 0, 2, 5 and 7.The initial mean pH value was 7.12±0.02 (SD) for all PC and dropped to 6.89, 6.85, 6.77 and 6.61 for group I to IV respectively, at day 7. A significant correlation (Spearman rank test) between low pH values and high leukocytes was found. The same significant positive correlation was observed between high leukocyte concentrations and high glucose consumption and high lactate production and LDH release during storage.These results show that the amount of leukocytes in PC has a significant contribution to the detrimental effect on pH during platelet storage. It is therefore important to prepare PC with a leukocyte count lower than 10 . Moreover the risk of alloimmunisation against HLA antigens will be diminished.

scholarly journals Therapeutic effectiveness of frozen platelet concentrates for transfusion

Blood ◽  
1981 ◽  
Vol 57 (2) ◽  
pp. 243-249 ◽  
Author(s):  
HM Lazarus ◽  
EA Kaniecki-Green ◽  
SE Warm ◽  
M Aikawa ◽  
RH Herzig
Keyword(s):  
Vapor Phase ◽  
Platelet Concentrate ◽  
In Vitro Tests ◽  
Platelet Concentrates ◽  
Emergency Situations ◽  
Ultrastructural Studies ◽  
Significant Difference ◽  
Before And After ◽  
The Difference

Abstract Six patients received platelet concentrate transfusions from their HLA- identical siblings. Platelet concentrates were administered either fresh, or after being frozen in 10% dimethylsulfoxide, at a slow controlled rate (1 degree C/min) or rapidly (approximately 8 degrees C/min) in the vapor-phase of a liquid nitrogen refrigerator. The median freeze-thaw loss was 13.5%. The mean 1-hr and 20-hr corrected increments in platelet count were calculated for fresh platelet concentrates transfused before and after transfusion with controlled- rate frozen and vapor-phase frozen platelet concentrates. There was no significant difference among the first and second transfusion of fresh platelet concentrates, nor was the difference observed between fresh and controlled-rate frozen platelet concentrates significant. The difference between fresh and vapor-phase frozen platelet concentrates, and between controlled-rate frozen and vapor-phase frozen platelet concentrates were highly significant (p < 0.01). In vitro tests of aggregation using ristocetin and platelet ultrastructural studies paralleled the transfusion experience. Our results indicate that HLA- identical platelet concentrates can be successfully frozen and thawed for transfusion if a slow, controlled rate of freezing is employed. The use of HLA-identical frozen platelet concentrates may be important in emergency situations for the refractory patient and potentially for the establishment of a platelet concentrate bank.

The Use of Functional and Quantitative Assays to Study Glycoprotein Ib in Platelets Stored Under Various In Vitro Conditions

1984 ◽  
Vol 52 (03) ◽  
pp. 271-275 ◽  
Author(s):  
M Ann Taylor ◽  
D J Anstee
Keyword(s):  
Storage Conditions ◽  
The Other ◽  
Membrane Glycoprotein ◽  
Platelet Concentrates ◽  
Glycoprotein Ib ◽  
Platelet Surface ◽  
Maclura Pomifera ◽  
In Vitro Storage ◽  
Blood Banks

SummaryThere is much evidence to suggest that platelet membrane glycoprotein Ib is involved in the haemostatic function of platelets and it has been suggested that loss of this glycoprotein may occur during in vitro storage of platelet concentrates. In this study two quantitative radioimmunoassays were developed to measure the content of glycoprotein lb in platelets stored under a range of conditions used in blood banks. One assay involved the use of iodinated Maclura pomifera lectin and the other the binding of a monoclonal antibody (AN51) specific for glycoprotein lb. The results showed that there was no significant reduction in the glycoprotein Ib content of platelets under the storage conditions used. These results suggest that any loss of haemostatic effectiveness which occurs on in vitro storage of platelet concentrates is not attributable to a selective loss of glycoprotein Ib from the platelet surface.

In vitro evaluation of metabolic changes and residual platelet responsiveness in photochemical treated and gamma-irradiated single-donor platelet concentrates during long-term storage

Transfusion ◽  
2007 ◽  
Vol 47 (4) ◽  
pp. 653-665 ◽  
Author(s):  
Torunn O. Apelseth ◽  
Øystein Bruserud ◽  
Tore Wentzel-Larsen ◽  
Anne M. Bakken ◽  
Solfrid Bjørsvik ◽  
...  
Keyword(s):  
Metabolic Changes ◽  
Platelet Concentrates ◽  
In Vitro Evaluation ◽  
Long Term Storage ◽  
Single Donor ◽  
Gamma Irradiated ◽  
Term Storage

scholarly journals Berberine Improved Aldo-Induced Podocyte Injury via Inhibiting Oxidative Stress and Endoplasmic Reticulum Stress Pathways both In Vivo and In Vitro

2016 ◽  
Vol 39 (1) ◽  
pp. 217-228 ◽  
Author(s):  
Bin Wang ◽  
Xianlin Xu ◽  
Xiaozhou He ◽  
Zhigang Wang ◽  
Min Yang
Keyword(s):  
Oxidative Stress ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Annexin V ◽  
Isoquinoline Alkaloid ◽  
Sprague Dawley ◽  
Podocyte Injury ◽  
Transmission Electron

Background/Aims: Berberine, a naturally occurring isoquinoline alkaloid, acts against oxidative stress (OS) and endoplasmic reticulum stress (ERS), both of which are responsible for Aldosterone (Aldo) -induced podocyte injury. However, the direct effects of berberine on Aldo-induced OS, ERS, and podocyte injury are not well defined. Methods: Uninephrectomized Sprague-Dawley rats were given 1% NaCl (salt) in their water and an Aldo infusion (0.75 µg/h) for 28 days to induce podocyte injury in the Aldo group. In the Aldo/berberine group, in addition to Aldo infusion, rats were administered 150 mg/kg berberine per day by gastric gavage for 4 weeks. Podocytes were incubated in media containing either buffer or Aldo in the presence or absence of berberine for variable time periods. The kidney tissues and podocytes were then investigated using morphological analysis, immunohistochemistry, transmission electron microscopy, western blot, DHE staining, DCFDA fluorescence, and Annexin V staining. Results: Here, we have reported that berberine attenuated Aldo-induced OS, ERS, and podocyte injury both in vivo and in vitro. Additionally, berberine treatment improved the extensive fusion of foot processes in electron micrographs resulting from Aldo/salt infusion in rats. Conclusion: Berberine may be examined as an effective agent against Aldo-induced podocyte injury.

scholarly journals Therapeutic effectiveness of frozen platelet concentrates for transfusion

Blood ◽  
1981 ◽  
Vol 57 (2) ◽  
pp. 243-249
Author(s):  
HM Lazarus ◽  
EA Kaniecki-Green ◽  
SE Warm ◽  
M Aikawa ◽  
RH Herzig
Keyword(s):  
Vapor Phase ◽  
Platelet Concentrate ◽  
In Vitro Tests ◽  
Platelet Concentrates ◽  
Emergency Situations ◽  
Ultrastructural Studies ◽  
Significant Difference ◽  
Before And After ◽  
The Difference

Six patients received platelet concentrate transfusions from their HLA- identical siblings. Platelet concentrates were administered either fresh, or after being frozen in 10% dimethylsulfoxide, at a slow controlled rate (1 degree C/min) or rapidly (approximately 8 degrees C/min) in the vapor-phase of a liquid nitrogen refrigerator. The median freeze-thaw loss was 13.5%. The mean 1-hr and 20-hr corrected increments in platelet count were calculated for fresh platelet concentrates transfused before and after transfusion with controlled- rate frozen and vapor-phase frozen platelet concentrates. There was no significant difference among the first and second transfusion of fresh platelet concentrates, nor was the difference observed between fresh and controlled-rate frozen platelet concentrates significant. The difference between fresh and vapor-phase frozen platelet concentrates, and between controlled-rate frozen and vapor-phase frozen platelet concentrates were highly significant (p < 0.01). In vitro tests of aggregation using ristocetin and platelet ultrastructural studies paralleled the transfusion experience. Our results indicate that HLA- identical platelet concentrates can be successfully frozen and thawed for transfusion if a slow, controlled rate of freezing is employed. The use of HLA-identical frozen platelet concentrates may be important in emergency situations for the refractory patient and potentially for the establishment of a platelet concentrate bank.

scholarly journals The Inhibition of Autophagy Flux Mediated by Neferine Promoted the Apoptosis of HNSCC through the Accumulation of p62/SQSTM1

2020 ◽  
Author(s):  
Fengshuo Zhu ◽  
Xiaoguang Li ◽  
Xiao Tang ◽  
Junjian Jiang ◽  
Yu Han ◽  
...  
Keyword(s):  
Therapeutic Option ◽  
Annexin V ◽  
Chemotherapeutic Agents ◽  
Antitumor Effects ◽  
Autophagy Flux ◽  
Jnk Pathway ◽  
Transmission Electron ◽  
Bisbenzylisoquinoline Alkaloid ◽  
Induced Apoptosis

Abstract Background: Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignancies worldwide. Patients usually have very poor prognosis because of metastasis and chemoresistance. There is an imperative need to explore more effective chemotherapeutic agents. Neferine, a bisbenzylisoquinoline alkaloid isolated from the seed embryo of Lotus, exerts antitumor effects via regulating apoptosis and autophagy pathways, which becomes a potential therapeutic option for HNSCC. Methods: Cell viability and proliferation was determined by the CCK8 and colony formation assay. Cell cycle and apoptosis analysis were assessed by flow cytometry with Annexin V-FITC/PI staining. Intracellular ROS levels were determined by the DCFH-DA fluorescence. Autophagy flux was detected by transmission electron microscopy, transfected GFP-RFP-LC3 fluorescence and western blot for related markers in the presence or absence of neferine and chloroquine. Results: Neferine inhibited the growth and induce the apoptosis of HNSCC cells both in vitro and vivo. Further analysis revealed that neferine activated ASK1/JNK pathway by increasing reactive oxygen species, then induced apoptosis of and regulated canonical autophagy in HNSCC cells. The study also revealed the novel pro-apoptosis mechanism through the activation of Caspase8 mediated by p62 due to the inhibition of autophagy flux. Conclusions: These findings explore the anti-cancer effect of neferine, providing new insights into the crosstalk between apoptosis and autophagy mediated by p62 in HNSCC. The inhibition of autophagy flux by neferine might be a potential adjuvant therapy to HNSCC.

scholarly journals Controlled Release of Ursodeoxycholic Acid from Pullulan Acetate Nanoparticles to Modulate Glutamate-Induced Excitotoxicity in PC-12 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Kwang Sik Yu ◽  
Jun Young Oh ◽  
Min Cheol Kim ◽  
Seong Hee Kang ◽  
Nam Seob Lee ◽  
...  
Keyword(s):  
Ursodeoxycholic Acid ◽  
Neuronal Damage ◽  
Annexin V ◽  
In Vitro Study ◽  
Poly Vinyl Alcohol ◽  
Neuroprotective Effects ◽  
Transmission Electron ◽  
Pullulan Acetate ◽  
Related Proteins

The neuroprotective effects of the ursodeoxycholic acid- (UDCA-) loaded pullulan acetate (PA) (UDCA-PA) nanospheres stabilized by poly(vinyl alcohol) (PVA) were identified by in vitro study. The UDCA-PA nanospheres were constructed by nanoemulsion process. The UDCA-PA nanospheres were analyzed using Fourier transform-infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). Then, the UDCA-PA nanospheres were used to treat PC-12 neuronal cells, which were formerly triggered by glutamate-induced excitotoxicity. As a result, the cells treated with the UDCA-PA nanospheres showed higher survival rate against glutamate-induced excitotoxicity. Furthermore, the UDCA-PA nanospheres decreased immunoreactivity of Annexin V, a membrane marker for apoptotic cells, in PC-12 with glutamate-induced injury. Particularly, the UDCA-PA nanospheres decreased the level of apoptosis-related proteins such as caspase-3. Taken together, the UDCA-PA nanospheres increased neuroprotective effects against glutamate-induced neuronal damage via inhibition of apoptosis at low concentration.

scholarly journals Selection of histocompatible apheresis platelet donors by cross- matching random donor platelet concentrates

Blood ◽  
1992 ◽  
Vol 79 (2) ◽  
pp. 527-531
Author(s):  
BA O'Connell ◽  
EJ Lee ◽  
K Rothko ◽  
MA Hussein ◽  
CA Schiffer
Keyword(s):  
Solid Phase ◽  
Hla Typing ◽  
Platelet Concentrates ◽  
Hla Antibodies ◽  
Single Donor ◽  
Large Numbers ◽  
Cross Matching ◽  
Selection Of

It can be impossible to identify compatible platelet donors for alloimmunized patients whose HLA type cannot be determined or who have uncommon HLA types. We have previously shown that histocompatible donors can be rapidly identified by “mass screening” of platelet concentrates (PC), which are readily available in all blood banks, using a solid-phase adherence platelet cross-matching technique. Compatible PC were given to five alloimmunized patients with multispecific HLA antibodies refractory to random donor (RD) PC and selected single-donor platelet transfusions. After transfusions which produced satisfactory responses, we identified the original whole blood donors to serve as apheresis donors. Thus, the donors selected were compatible in vitro by cross-matching, and in vivo by transfusion. Only 3% to 13% of PC cross-matched for these alloimmunized patients were potentially compatible and it was necessary to screen large numbers (65 to 205 U) of PC per patient. Eighteen of 22 PC-selected transfusions produced satisfactory increments, allowing selection of 12 donors, all of whom were willing to undergo apheresis. Ten of 12 of these single- donor transfusions were successful; the two unsuccessful transfusions were infused 2 weeks after the initial PC cross-match and were still compatible with the original serum, but incompatible with more recent serum, demonstrating a change in antibody reactivity. The HLA types of the successful single donors selected by PC cross-matching differed widely from the patients' HLA types and, therefore, these donors would not have been selected by standard approaches using HLA typing. Cross- matching large numbers of RD PC for the identification of apheresis donors is helpful in the management of the alloimmunized patient and may be of particular utility for blood centers that do not have access to HLA-typed donor pools.

Sign in / Sign up

Export Citation Format

Share Document