Soluble CD40L and CD62P levels differ in single‐donor apheresis platelet concentrates and buffy coat–derived pooled platelet concentrates

Transfusion ◽  
2018 ◽  
Vol 59 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Caroline Sut ◽  
Sofiane Tariket ◽  
Chaker Aloui ◽  
Charles‐Antoine Arthaud ◽  
Marie‐Ange Eyraud ◽  
...  
Keyword(s):  
Buffy Coat ◽  
Platelet Concentrates ◽  
Single Donor ◽  
Soluble Cd40l

scholarly journals Evaluation of the in vitro Function of Platelet Concentrates from Pooled Buffy Coats or Apheresis

2020 ◽  
Vol 47 (4) ◽  
pp. 314-325
Author(s):  
Sarah Anna Fiedler ◽  
Klaus Boller ◽  
Ann-Christine Junker ◽  
Christel Kamp ◽  
Anneliese Hilger ◽  
...  
Keyword(s):  
Shear Stress ◽  
Annexin V ◽  
Storage Conditions ◽  
Platelet Concentrate ◽  
Platelet Concentrates ◽  
Stability Parameters ◽  
Single Donor ◽  
Transmission Electron ◽  
Soluble Cd40l

Background: Platelet concentrates play an important role in transfusion medicine. Their short lifespan and lack of robustness require efforts to ensure adequate product quality. In this study, we compared the in vitro quality of the main concentrate types, pooled platelet concentrate (PPC) from whole blood donations, and platelet concentrate from single-donor apheresis (APC). Methods: Twenty PPCs and 20 APCs prepared in plasma were analyzed on days 2, 4, and 7 of storage. Variables related to metabolism, degranulation, platelet aggregation, P-selectin expression, and annexin V binding were analyzed. Morphology was assessed by transmission electron microscopy of ultrathin sections. A microfluidic device was applied to test the effects of shear stress on platelet function. Results: The metabolic parameters indicated stable storage conditions throughout the 7-day period. The resting discoid form was the prevailing morphology on days 2 and 4 in the PPCs and APCs. Chemokine release and receptor shedding of soluble P-selectin and soluble CD40L equally increased in PPCs and APCs. Aggregation responses to ADP and collagen were heterogeneous, with marked losses in collagen responsiveness on day 4 in individual concentrates. Baseline expression of P-selectin in PPCs and APCs was low, and inducibility of P-selectin was well preserved until day 4. Under shear stress, equal adhesiveness and stability were found with platelets from PPCs and APCs. Conclusions: Platelets from PPCs and APCs showed similar in vitro function and stability parameters. However, platelet concentrates presented a high variability and individual concentrates an impaired functional capability. Identifying the factors contributing to this would help increase product reliability.

A clinical and laboratory study of platelet concentrates produced by pooled buffy coat and single donor apheresis technologies

1995 ◽  
Vol 16 (2) ◽  
pp. 187-188 ◽  
Author(s):  
D. Bishop ◽  
N. Tandy ◽  
N. Anderson ◽  
H. Bessos ◽  
M.J. Seghatchian
Keyword(s):  
Laboratory Study ◽  
Buffy Coat ◽  
Platelet Concentrates ◽  
Single Donor

In vitro Evaluation of Buffy-Coat-Derived Platelet Concentrates Stored in a Synthetic Medium

Vox Sanguinis ◽  
1991 ◽  
Vol 60 (1) ◽  
pp. 16-22 ◽  
Author(s):  
R. Fijnheer ◽  
H.A. Veldman ◽  
A.J.M. Eertwegh ◽  
C.W.N. Gouwerok ◽  
C.H.E. Homburg ◽  
...  
Keyword(s):  
Synthetic Medium ◽  
Buffy Coat ◽  
Platelet Concentrates ◽  
In Vitro Evaluation

scholarly journals Delayed alloimmunization using random single donor platelet transfusions: a prospective study in thrombocytopenic patients with acute leukemia

Blood ◽  
1983 ◽  
Vol 62 (2) ◽  
pp. 473-479 ◽  
Author(s):  
J Gmur ◽  
A von Felten ◽  
B Osterwalder ◽  
H Honegger ◽  
A Hormann ◽  
...  
Keyword(s):  
Acute Leukemia ◽  
Prospective Study ◽  
Randomized Study ◽  
Platelet Concentrates ◽  
Single Donor ◽  
Time Period ◽  
A Prospective Study ◽  
Platelet Transfusions

Abstract A randomized study was performed in 54 thrombocytopenic patients with acute leukemia. Alloimmunization of recipients of random multiple-donor platelet concentrates (MD group) was compared to that of patients receiving random single-donor platelets (SD group). In the SD patients, formation of alloantibodies (mostly anti-HLA) occurred less frequently (p less than 0.002), after a longer time period (p less than 0.002), and after a higher number of transfusions (p less than 0.005) as compared to MD patients. SD patients also became refractory to random platelets less frequently (p less than 0.005), after a longer time period, and after a higher number of transfusions (p less than 0.02). In SD patients, the increments after the first and the last transfusion were in the same range, whereas in MD patients, the 1-hr (p less than 0.001) and the 24-hr (p less than 0.025) increments decreased from the first to the last transfusion. Thus, the use of random SD platelet transfusions postponed alloimmunization.

PRODUCTION OF PLATELETS FOR TRANSFUSION - CURRENT METHODS AND PROBLEMS TO BE SOLVED

1987 ◽  
Author(s):  
M K Elias ◽  
C Th Smit Sibinga
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Synthetic Medium ◽  
Buffy Coat ◽  
Cotton Wool ◽  
Single Donor ◽  
Increased Risk ◽  
Multiple Donors ◽  
And Storage ◽  
Selection Of

Initially, whole blood or platelet rich plasma were used as sources of platelets. Nowadays the methods of platelet concentrates (pc) production adopted in Blood Banks include the traditional method of platelet preparation by differential centrifugation of units of whole blood, besides the much more sophisticated technique of extracorporeal collection of pc with improved immunological compatibility.Manually pc are produced by the platelet rich plasmamethod, the buffy coat method and multiple bag plateletapheresis. The machine collection of pc is done by plateletapheresis or platelet elutriation, with different degrees of automation.The standard manual method remains quantitatively the most important source of platelets.However, there are major concerns:-the need of multiple donors-The high contamination with white cells, predominantly lymphocytes-these pc are depleted from larger and more active platelets, as these are sedimented with the red cells-increased risk of bacterial contamination. To solve these problems there are some potention solutions:-use of single donor collectioon techniques-depletion of leucocytes by:a.elutriation of platelets from the buffy coatb.filtration of random pc through cotton wool columnc. prostacyclin inhibition of platelet aggregation followed by cellulose acetate filtrationd.filtration on elutriated platelets through cotton wool-use of a platelet synthetic medium void of glucose for resuspension and storage of pc to prevent lactate accumulation and pH fall-use of closed sterile harness systems to collect platelets by surge plateletapheresis, which allows extended storage of leucocyte depleted pc.Selection of the most appropriate platelet concentrate depends on the interrelationship of many factors:1) yield 2) function 3) viability after storage 4) afety 5) purity 6) potency 7) efficacy (recovery, survival and haemostatic capacity).

Pathogen reduction treatment alters the immunomodulatory capacity of buffy coat-derived platelet concentrates

Transfusion ◽  
2013 ◽  
Vol 54 (3) ◽  
pp. 577-584 ◽  
Author(s):  
Yen S. Loh ◽  
Lacey Johnson ◽  
Matthew Kwok ◽  
Denese C. Marks
Keyword(s):  
Buffy Coat ◽  
Platelet Concentrates ◽  
Reduction Treatment ◽  
Immunomodulatory Capacity ◽  
Pathogen Reduction
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