scholarly journals Frequency of Mia (MNS7) and Classification of Mia-Positive Hybrid Glycophorins in an Australian Blood Donor Population

2019 ◽  
Vol 47 (4) ◽  
pp. 279-287
Author(s):  
Genghis H. Lopez ◽  
Brett Wilson ◽  
Robyn M. Turner ◽  
Glenda M. Millard ◽  
Nicole S. Fraser ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donor ◽  
Asian Population ◽  
Blood Group System ◽  
Genes Encoding ◽  
Donor Population ◽  
Melting Analysis ◽  
Hybrid Genes ◽  
High Degree ◽  
Group Profile

Background: MNS blood group system genes GYPA and GYPB share a high degree of sequence homology and gene structure. Homologous exchanges between GYPA and GYPB form hybrid genes encoding hybrid glycophorins GP(A-B-A) and GP(B-A-B). Over 20 hybrid glycophorins have been characterised. Each has a distinct phenotype defined by the profile of antigens expressed including Mia. Seven hybrid glycophorins carry Mia and have been reported in Caucasian and Asian population groups. In Australia, the population is diverse; however, the prevalence of hybrid glycophorins in the population has never been determined. The aims of this study were to determine the frequency of Mia and to classify Mia-positive hybrid glycophorins in an Australian blood donor population. Method: Blood samples from 5,098 Australian blood donors were randomly selected and screened for Mia using anti-Mia monoclonal antibody (CBC-172) by standard haemagglutination technique. Mia-positive red blood cells (RBCs) were further characterised using a panel of phenotyping reagents. Genotyping by high-resolution melting analysis and DNA sequencing were used to confirm serology. Result: RBCs from 11/5,098 samples were Mia-positive, representing a frequency of 0.22%. Serological and molecular typing identified four types of Mia-positive hybrid glycophorins: GP.Hut (n = 2), GP.Vw (n = 3), GP.Mur (n = 5), and 1 GP.Bun (n = 1). GP.Mur was the most common. Conclusion: This is the first comprehensive study on the frequency of Mia and types of hybrid glycophorins present in an Australian blood donor population. The demographics of Australia are diverse and ever-changing. Knowing the blood group profile in a population is essential to manage transfusion needs.

Phenotypic and allelic profile of ABO and Rhésus D blood group system among blood donor in Antananarivo

2012 ◽  
Vol 39 (6) ◽  
pp. 477-479 ◽  
Author(s):  
Z. A. Randriamanantany ◽  
D. H. Rajaonatahina ◽  
F. E. Razafimanantsoa ◽  
M. T. Rasamindrakotroka ◽  
R. Andriamahenina ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Donor ◽  
Blood Group System ◽  
Allelic Profile ◽  
Group System

scholarly journals Identification of recombination events resulting in three hybrid genes encoding human MiV, MiV(J.L.), and Sta glycophorins

Blood ◽  
1991 ◽  
Vol 77 (8) ◽  
pp. 1813-1820 ◽  
Author(s):  
CH Huang ◽  
OO Blumenfeld
Keyword(s):  
Reverse Direction ◽  
Crossing Over ◽  
Blood Group System ◽  
Hindiii Site ◽  
Direct Dna Sequencing ◽  
The Third ◽  
Oligonucleotide Hybridization ◽  
Genes Encoding ◽  
Exon 4 ◽  
Hybrid Genes

Abstract MiV, MiV(J.L.), and Sta glycophorins specify the respective variant phenotypes of the human MNSs blood group system. We report that unequal but homologous crossing-over between alpha and delta glycophorin genes results in three hybrid genes encoding MiV, MiV(J.L.), and Sta glycophorins. Restriction mapping and allele-specific oligonucleotide hybridization grossly defined the third intron as the probable crossing- over site and showed that MiV and MiV(J.L.) genes are arranged in the same 5′ alpha-delta 3′ frame whereas Sta gene is in a reciprocal 5′delta-alpha 3′ configuration. Genomic sequences spanning the extracellular domain exons 2 to 4 were amplified from each variant gene by polymerase chain reaction and determined by direct DNA sequencing. Comparison of nucleotide sequences encompassing the third intron showed that the three hybrid genes differed in location of crossing-over sites. The alpha-delta breakpoints in MiV and MiV(J.L.) genes were localized to the 3′ end of the HindIII site downstream from exon 3 and to the 5′ end immediately upstream from exon 4, respectively, whereas the delta-alpha breakpoint in Sta gene resided in between. An AAAGT sequence oriented in either forward or reverse direction was identified within the crossing-over region of each hybrid gene whose surrounding sequences bear a strong local strand asymmetry. The single nucleotide substitution in exon 4 of MiV and MiV(J.L.) genes (ACG [Thr] to ATG [Met]) demonstrated that the two genes differed in the delta glycophorin alleles that must have participated in the recombination.

scholarly journals DISTRIBUTION OF BLOOD GROUP IN BLOOD DONOR POPULATION IN WEST BENGAL

2018 ◽  
Vol 7 (31) ◽  
pp. 3445-3447
Author(s):  
Arpita Chatterjee Halder ◽  
Alpana De ◽  
Saibendu Kumar Lahiri
Keyword(s):  
Blood Group ◽  
Blood Donor ◽  
West Bengal ◽  
Donor Population

Distribution of Blood Types and ABO Gene Frequencies in Egypt

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S153-S153
Author(s):  
Mohamed Abdelmonem ◽  
Amira Fyala ◽  
Ayman Boraik ◽  
Mohamed Shedid ◽  
Amira Husseiny Mohamed ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Groups ◽  
Transfusion Practice ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Gene Frequencies ◽  
Future Health ◽  
Egyptian Population ◽  
Naturally Occurring ◽  
Donor Population

Abstract ABO blood group was first discovered by Landsteiner in 1901. Currently, there are more than 30 blood group systems, but ABO system remains the most clinically important of all blood groups in transfusion practice. The ABO blood group system antibodies are naturally occurring without any exposure to RBCs through transfusion or pregnancy, unlike the other blood group systems. Method The study was performed on a total of 40,591 healthy blood donors in Egypt. ABO and Rh (D) groupings were performed on all donors’ samples. Data on the frequency of ABO and Rh(D) blood groups were reported in numbers and percentages. Results The study showed that type A is the most common blood group (35.12%) in Egypt followed by O at 31.94%, followed by B at 23.12%, while AB had the least prevalence at 9.74%; A > O > B > AB. Our study showed that 91.78% of the donor population were Rh positive and 8.22% were Rh negative. The frequencies of the IA, IB, and IO alleles were calculated using the Hardy-Weinberg law of equilibrium. The calculated gene frequencies are 0.2537 for IA (p), 0.1812 for IB (q), and 0.5651 for IO (r). In the Egyptian population, O (r) records the highest value, followed by B (q) and A (p); O > B > A. The homozygous types were as follows: OO, 31.94%; AA, 6.43%; and BB, 3.28%. The heterozygous types were AO, 28.67%; BO, 20.47%; and AB, 9.78%. Conclusions The study provides the first accurate ABO gene frequency data as well as information on the distribution of ABO blood group Rh groups of various alleles in the Egyptian population. This information is very helpful in the effective management of the blood bank inventory. It will help transfusion services planning for future health challenge and improve blood transfusion practice.

The Rh blood group system: a review

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 375-387 ◽  
Author(s):  
Neil D. Avent ◽  
Marion E. Reid
Keyword(s):  
Blood Group ◽  
Blood Group System ◽  
Group System ◽  
System A ◽  
Extensive Documentation ◽  
Genes Encoding ◽  
Rh Proteins ◽  
D Antigen ◽  
Molecular Bases ◽  
Rh Blood Group

The Rh blood group system is one of the most polymorphic and immunogenic systems known in humans. In the past decade, intense investigation has yielded considerable knowledge of the molecular background of this system. The genes encoding 2 distinct Rh proteins that carry C or c together with either E or e antigens, and the D antigen, have been cloned, and the molecular bases of many of the antigens and of the phenotypes have been determined. A related protein, the Rh glycoprotein is essential for assembly of the Rh protein complex in the erythrocyte membrane and for expression of Rh antigens. The purpose of this review is to provide an overview of several aspects of the Rh blood group system, including the confusing terminology, progress in molecular understanding, and how this developing knowledge can be used in the clinical setting. Extensive documentation is provided to enable the interested reader to obtain further information.

scholarly journals ABO, Rhesus blood groups and transfusion-transmitted infections among blood donors in Gabon

2018 ◽  
Vol 13 (1) ◽  
pp. 12 ◽  
Author(s):  
Christ-Dominique Ngassaki-Yoka ◽  
Jophrette Mireille Ntsame Ndong ◽  
Cyrille Bisseye
Keyword(s):  
Blood Group ◽  
Blood Donor ◽  
Representative Sample ◽  
Blood Donors ◽  
Blood Groups ◽  
Abo Blood Group ◽  
Blood Group Antigens ◽  
Abo Blood Groups ◽  
Donor Population ◽  
Monospecific Antisera

Background: Few studies focused on the study of blood groups in Gabon. This study aimed to determine the phenotypic frequency of ABO and Rhesus antigens in blood donors of Libreville and to assess the association between ABO blood groups and transfusion-transmitted infections.Materials and Methods: The study of ABO and Rhesus blood groups concerned 4,744 blood donors. ABO and Rhesus phenotyping were obtained using monoclonal monospecific antisera: anti-A, anti-B, anti-AB, anti-D, anti-E, anti-C, anti-c, and anti-e with an automate (QWALYS® 3, DIAGAST, France) or a card gel (ID Card, BIO-RAD) according to manufacturer’s instructions.Results: The phenotypic frequency of blood group antigens A, B, AB and O were respectively 21.0%; 17.6%; 2.6% and 58.9%. Those of rhesus antigens D, d, C, c, E and e were 97.7%; 2.3%; 15.9%; 99.9%; 17.6%; 99.3%, respectively. The prevalence of ABO and Rh antigens in Gabonese donors reported here are significantly different from those of neighboring countries. No association was found between the prevalence of HIV, HCV and syphilis and ABO blood groups. Instead, HBV seroprevalence was twice as high among non-O blood groups donors compared with blood group O donors [OR = 2 (CI 1.26 to 3.2), p = 0.003].Conclusions: This study provides new data on phenotypic frequency of ABO and Rh blood groups in a representative sample of the Gabonese blood donor population. It suggests a significant association between ABO blood group and HBV infection.

scholarly journals Rare blood donors with irregular antibodies

2013 ◽  
Vol 66 (7-8) ◽  
pp. 331-334
Author(s):  
Mirjana Krga-Milanovic ◽  
Nevenka Bujandric ◽  
Natasa Milosavljevic-Knezevic
Keyword(s):  
Blood Transfusion ◽  
Red Blood Cells ◽  
Blood Group ◽  
Blood Donor ◽  
Blood Cells ◽  
High Frequency ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Group System ◽  
Group System

Introduction. Blood groups are inherited biological characteristics that do not change throughout life in healthy people. Blood groups represent antigens found on the surface of red blood cells. Kell blood group system consists of 31 antigens. Kell antigen (K) is present in 0.2% of the population (the rare blood group). Cellano antigen is present in more than 99% (the high-frequency antigen). These antigens have a distinct ability to cause an immune response in the people after blood transfusion or pregnancy who, otherwise, did not have them before. Case Report. This paper presents a blood donor with a rare blood group, who was found to have an irregular antibody against red blood cells by indirect antiglobulin test. Further testing determined the specificity of antibody to be anti-Cellano. The detected antibody was found in high titers (1024) with erythrocyte phenotype Kell-Cellano+. The blood donor was found to have a rare blood group KellKell. This donor was excluded from further blood donation. It is difficult to find compatible blood for a person who has developed an antibody to the high-frequency antigen. The donor?s family members were tested and Cellano antigen was detected in her husband and child. A potential blood donor was not found among the family members. There was only one blood donor in the Register of blood donors who was compatible in the ABO and Kell blood group system. Conclusion. For the successful management of blood transfusion it is necessary to establish a unified national register of donors of rare blood groups and cooperate with the International Blood Group Reference Laboratory in Bristol with the database that registers donors of rare blood groups from around the world.

scholarly journals Identification of recombination events resulting in three hybrid genes encoding human MiV, MiV(J.L.), and Sta glycophorins

Blood ◽  
1991 ◽  
Vol 77 (8) ◽  
pp. 1813-1820
Author(s):  
CH Huang ◽  
OO Blumenfeld
Keyword(s):  
Reverse Direction ◽  
Crossing Over ◽  
Blood Group System ◽  
Hindiii Site ◽  
Direct Dna Sequencing ◽  
The Third ◽  
Oligonucleotide Hybridization ◽  
Genes Encoding ◽  
Exon 4 ◽  
Hybrid Genes

MiV, MiV(J.L.), and Sta glycophorins specify the respective variant phenotypes of the human MNSs blood group system. We report that unequal but homologous crossing-over between alpha and delta glycophorin genes results in three hybrid genes encoding MiV, MiV(J.L.), and Sta glycophorins. Restriction mapping and allele-specific oligonucleotide hybridization grossly defined the third intron as the probable crossing- over site and showed that MiV and MiV(J.L.) genes are arranged in the same 5′ alpha-delta 3′ frame whereas Sta gene is in a reciprocal 5′delta-alpha 3′ configuration. Genomic sequences spanning the extracellular domain exons 2 to 4 were amplified from each variant gene by polymerase chain reaction and determined by direct DNA sequencing. Comparison of nucleotide sequences encompassing the third intron showed that the three hybrid genes differed in location of crossing-over sites. The alpha-delta breakpoints in MiV and MiV(J.L.) genes were localized to the 3′ end of the HindIII site downstream from exon 3 and to the 5′ end immediately upstream from exon 4, respectively, whereas the delta-alpha breakpoint in Sta gene resided in between. An AAAGT sequence oriented in either forward or reverse direction was identified within the crossing-over region of each hybrid gene whose surrounding sequences bear a strong local strand asymmetry. The single nucleotide substitution in exon 4 of MiV and MiV(J.L.) genes (ACG [Thr] to ATG [Met]) demonstrated that the two genes differed in the delta glycophorin alleles that must have participated in the recombination.

Apparent Depression of Antigens of the Kell Blood Group System Associated with Autoimmune Acquired Haemolytic Anaemia

Vox Sanguinis ◽  
1972 ◽  
Vol 23 (6) ◽  
pp. 528-536
Author(s):  
Halina Seyfield ◽  
Barbara Górska ◽  
S. Maj ◽  
T. Sylwestrowicz ◽  
Carolyn Giles ◽  
...  
Keyword(s):  
Blood Group ◽  
Haemolytic Anaemia ◽  
Blood Group System ◽  
Group System
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