scholarly journals Rare blood donors with irregular antibodies

2013 ◽  
Vol 66 (7-8) ◽  
pp. 331-334
Author(s):  
Mirjana Krga-Milanovic ◽  
Nevenka Bujandric ◽  
Natasa Milosavljevic-Knezevic
Keyword(s):  
Blood Transfusion ◽  
Red Blood Cells ◽  
Blood Group ◽  
Blood Donor ◽  
Blood Cells ◽  
High Frequency ◽  
Blood Donors ◽  
Blood Groups ◽  
Blood Group System ◽  
Group System

Introduction. Blood groups are inherited biological characteristics that do not change throughout life in healthy people. Blood groups represent antigens found on the surface of red blood cells. Kell blood group system consists of 31 antigens. Kell antigen (K) is present in 0.2% of the population (the rare blood group). Cellano antigen is present in more than 99% (the high-frequency antigen). These antigens have a distinct ability to cause an immune response in the people after blood transfusion or pregnancy who, otherwise, did not have them before. Case Report. This paper presents a blood donor with a rare blood group, who was found to have an irregular antibody against red blood cells by indirect antiglobulin test. Further testing determined the specificity of antibody to be anti-Cellano. The detected antibody was found in high titers (1024) with erythrocyte phenotype Kell-Cellano+. The blood donor was found to have a rare blood group KellKell. This donor was excluded from further blood donation. It is difficult to find compatible blood for a person who has developed an antibody to the high-frequency antigen. The donor?s family members were tested and Cellano antigen was detected in her husband and child. A potential blood donor was not found among the family members. There was only one blood donor in the Register of blood donors who was compatible in the ABO and Kell blood group system. Conclusion. For the successful management of blood transfusion it is necessary to establish a unified national register of donors of rare blood groups and cooperate with the International Blood Group Reference Laboratory in Bristol with the database that registers donors of rare blood groups from around the world.

Forssman expression on human erythrocytes: biochemical and genetic evidence of a new histo-blood group system

Blood ◽  
2013 ◽  
Vol 121 (8) ◽  
pp. 1459-1468 ◽  
Author(s):  
Lola Svensson ◽  
Annika K. Hult ◽  
Robert Stamps ◽  
Jonas Ångström ◽  
Susann Teneberg ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Human Erythrocytes ◽  
Blood Group System ◽  
Group System ◽  
Glycolipid Antigen ◽  
Human Red Blood Cells ◽  
Key Points ◽  
Forssman Antigen

Key Points A new histo-blood group system was discovered, based on the identification of Forssman glycolipid antigen on human red blood cells. A newly described polymorphism in the GBGT1 gene activates the encoded enzyme to synthesize Forssman antigen.

scholarly journals Prevalence of ABO and Rh blood groups and their association with Transfusion-Transmissible Infections (TTIs) among Blood Donors in Islamabad, Pakistan

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Muhammad Yousaf Khan ◽  
Farwa Sijjeel ◽  
Ahmareen Khalid ◽  
Rukhshan Khurshid ◽  
Umm E Habiba ◽  
...  
Keyword(s):  
Viral Infection ◽  
Blood Group ◽  
Blood Donors ◽  
Blood Groups ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Syphilis Infection ◽  
Group System ◽  
Group A ◽  
Group B

Certain Rh positive blood groups showed a link between the ABO blood grouping and susceptibility to some infectious ailments. Study was carried out to find the association of ABO Blood Group System / Rh type with different viral infection. A retrospective observational study was carried in 7631 blood donors to find the association of ABO Blood Group System / Rh type with viral infection due to hepatitis B virus, hepatitis C virus, human immunodeficiency virus and Syphilis. Data of the study indicateF that the highest incidence of blood group in blood donor was B followed by O, A and AB. The incidence of HBsAg +ve, HCV Ab +ve and infection of Syphilis was also highest in donor with blood group B followed by O, A and AB. Blood group A were more susceptible to HIV Ab +ve followed by blood group B, O and AB. The group B donors had the greatest risk of viral infection with HBV, HCV, HIV, and Syphilis; followed by the groups O, A, and AB. Donors in group AB had the lowest incidence rate of all viral infection. The study revealed that blood group B is most prevalent among donors, followed by blood groups O, A, and AB. On the other side, the rate of HBV, HCV, HIV, and Syphilis infection was greatest among group B donors, followed by group O, group A, and group AB. Copyright(c) The Authors

An Insight into the General Trend of Blood Group Dissemination among Local Population of Muzaffarabad

2020 ◽  
pp. 39-43
Author(s):  
OJS Admin
Keyword(s):  
Blood Group ◽  
Disease Susceptibility ◽  
General Trend ◽  
Local Population ◽  
Blood Groups ◽  
Blood Group System ◽  
Group System ◽  
Insight Into

Blood groups ABO and Rhesus, constituting the most principal blood group system, are of key signicance for clinical and transfusion practices and are moreover, thought to be associated with disease susceptibility.

scholarly journals Synthetic peptides homologous to human glycophorins of the Miltenberger complex of variants of MNSs blood group system specify the epitopes for Hil, SJL, Hop, and Mur antisera

Blood ◽  
1991 ◽  
Vol 78 (9) ◽  
pp. 2456-2461 ◽  
Author(s):  
KK Johe ◽  
V Vengelen-Tyler ◽  
R Leger ◽  
OO Blumenfeld
Keyword(s):  
Blood Group ◽  
Blood Cells ◽  
Blood Group System ◽  
Junction Region ◽  
Group System ◽  
Erythrocyte Surface ◽  
Mnss Blood Groups ◽  
The Common ◽  
Expressed Sequence ◽  
Antigenic Epitopes

Abstract The antigenic epitopes of the MNSs blood groups are localized on alpha and delta glycophorins (glycophorins A and B) of the erythrocyte surface. Hil, SJL, Mur, and Hop antisera define the Miltenberger (Mi) complex of MiV, MiJ.L., MiIII, and MiVI variant serologic phenotypes of this blood group system. We report here the location of the epitopes for antibodies in these antisera. The antigens of these Mi classes are variant glycophorins that are hybrids of alpha and delta glycophorins in alpha-delta and delta-alpha-delta arrangements. The hybrid junctions give rise to novel polypeptide sequences not present in the parent glycophorins; in MiIII and MiVI this also includes an expressed sequence of the delta pseudoexon. These sequences are identical in the above Mi-glycophorins occurring in erythrocytes that share a common Mi determinant. Four peptides of 10 to 14 amino acids each were constructed to be homologous to the identical sequences; they were designated, “Hil”, “SJL”, “Mur”, and “Hop” to reflect the common determinant. The peptides were tested for inhibition of reaction of appropriate cells with the relevant antisera. The Hil peptide, outlining the alpha-delta s junction region in MiIII, MiV, and MiVI glycophorins, inhibited the reaction of respective erythrocytes (red blood cells [RBCs]) with anti-Hil. The SJL peptide, which differs from the Hil peptide by a single Thr----Met substitution, was specific for inhibition of the reaction of MiJ.L. RBCs with anti-SJL (an example of anti-S specific for such RBCs). The Hop peptide, which corresponds to the delta-alpha junction in MiVI glycophorin, inhibited the hemagglutination of MiVIII RBCs by anti-Hop. MiVI and MiVIII glycophorins share an identical sequence at that site. The Mur peptide, corresponding to a portion of the expressed pseudoexon sequence in MiIII and MiVI glycophorins, was specific for inhibition of the reaction of MiIII and MiVI RBCs with anti-Mur. The peptides had no effect on the hemagglutination of control MNSs RBCs by their respective antisera nor of unrelated Mi classes RBCs by antisera that distinguish these classes. We conclude that the alpha-delta junction in MiIII, MiV, and MiVI glycophorins outlines the epitopes for anti-Hil, the alpha- delta junction in MiJ.L. outlines the epitope for anti-SJL, the delta- alpha junction in MiVI constitutes the epitope for anti-Hop, and the expressed delta pseudoexon sequence in MiIII and MiVI constitutes the epitope for anti-Mur.

The Presence of FYAnull Alele of Duffy Blood Group System in Blood Donors and Individuals from a Malarial Endemic Region of Brazil.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2714-2714 ◽  
Author(s):  
Dante M. Langhi ◽  
Sergio R. Albuquerque ◽  
Dimas T. Covas ◽  
Clovis A. Perez ◽  
Jose O. Bordin
Keyword(s):  
Sequence Analysis ◽  
Blood Group ◽  
Blood Donors ◽  
Single Point Mutation ◽  
Binding Motif ◽  
Blood Group System ◽  
Endemic Region ◽  
Group System ◽  
Duffy Blood Group ◽  
Gata Box

Abstract BACKGROUND: Malaria is a virulent disease caused by the Plasmodium parasite. Innate resistance to malaria infections in humans is conferred by various blood group polymorphisms. The Duffy blood group system consists of Fya and Fyb antigens which are encoded by codominant alleles FYA and FYB. Four phenotypes are defined: Fy(a+b+), Fy(a+b−), Fy(a−b+) and Fy(a−b−). Erythrocytes of Duffy-negative individuals are resistant to invasion by P. vivax. In Blacks the Fy(a−b−) phenotype is associated with a single point mutation (-33T-C) in the GATA-1 binding motif for the erythroid promoter of FYB. STUDY DESIGN AND METHODS: We investigated the phenotypes and the genotypes of Duffy blood group system of 250 individuals living in a malarial endemic region (MER) in the state of Amazon (Brazil), and of 199 blood donors (BD) from a non-endemic region. The phenotyping was done by agglutination gel tests (DiaMed-Latino América) using anti-Fya and anti-Fyb reagents. The molecular analysis for FYA, FYB, FYBES (GATA box mutation nt -33T-C), and FYBWeak (mutations 265 C-T, and 298 G-A) alleles, were performed by PCR-RFLP. The PCR products were digested by Ban I for FYA and FYB identifications; by Sty I for GATA box mutation; Acy I and Mwo I for 265 C-T and 298 G-A mutations, respectively. Some samples that showed discrepancy between the phenotype and genotype results were examined by sequence analysis using the ABI PrismâBig Dyeä Terminator Cycle Sequencing Ready Reaction Kit” (Perkin Elmer), and the interpretation by the software ABI PRISMä 377 DNA Sequencer”, 3.3 version (Perkin Elmer). RESULTS: We found that 34/250 (13.6%) of 250 persons living in the MER and 37/199 (18.6%) of BD had phenotype and genotype discrepant results [Fy(a+b−) FYA/FYB]. In addition, we found that 16/34 (47%) of people living in the MER, and 4/37 (10.8%) of BD did not present the -33T-C mutation, the 265 C-T, or the 298 G-A mutations. The sequence analysis of 2 samples from persons from MER indicated the presence of -33T-C mutation in the FYA allele in one individual (1 FYA/FYB and W/M; FYA/FYB and M/M). Additionally, we detected that 18/34 (53%) of people living in the MER, and 33/37 (89.2%) of BD presented the -33T-C mutation. The sequence analysis of 5 samples indicated the presence of -33T-C mutation in the FYA allele in 4 cases [2 persons from MER and 2 from BD (FYA/FYB e M/M)]. CONCLUSION: Recently the mutation responsible for erythrocyte Duffy antigen-negativity [Fy(a−b−)] was demonstrated in FYA allele in a malarial endemic region of Papua New Guinea. The present data demonstrated the presence of the FYAnull allele not only in persons living in a malarial endemic region but also in Brazilian blood donors from non-endemic areas. In contrast with that which happens with the FYB allele, our results indicated that the presence of the -33T-C mutation in the FYA allele does not abolish the expression of the Fya antigen in the erythrocyte.

The rates of ABO, Rh, and Kell antigens in children with cancer.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e22007-e22007
Author(s):  
Andrey A. Maslov ◽  
Nailya Guskova ◽  
Ekaterina Guskova ◽  
Kristina Avanesova ◽  
Svetlana V. Belgova ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Subsequent Development ◽  
European Population ◽  
Blood Groups ◽  
Abo Blood Groups ◽  
Phenotypic Characteristics ◽  
Children With Cancer ◽  
K Antigen

e22007 Background: The purpose of the study was to analyze phenotypic characteristics of red blood cells by the AVBO, Rh and Kell systems in children with cancer. Methods: ABO and Rh blood groups were determined and erythrocyte antigens (D, С, с, Сw, Е, е, К, k) were typed (AutoVue Innova, USA) in blood samples of 114 children with solid tumors. Results: ABO blood groups distribution was as follows: A(II) > O(I) > B(III) > AB(IV) with A(II) prevalence. Rh(D)-positive phenotype was observed in 82 (71.9%) patients of 114: 47 (57.3%) boys and 35 (42.6%) girls. 32 (28.1%) patients of 114 were Rh(D)-negative: 15 (46.8%) boys and 17 (53.1%) girls. Only 8 (7%) children were Kell-positive, which was similar to the antigen prevalence in European population. 4 erythrocyte phenotypes were the most frequent in Rh(D)-positive patients: СсDееK− (34.1%), ССDееK− (22.0%), ccDEeK− (13.4%) and СсDЕеK− (11.0%). I.e., more than a half of children with oncopathologies (56.1%) had Kell-negative phenotypes, СсDееK− and ССDееK−. 86.4% of Rh(D)-positive patients had homozygous combinations of Rhesus antigens causing transfusion reactions - СС, сc, ЕЕ and ее. 18 (22.0%) of Rh(D)-positive patients were homozygous for the C antigen and 64 (78.0%), i.e. every third patient, had the c antigen. Children with the C antigen may be sensitized to the c antigen through blood transfusion with subsequent development of hemolytic complications. The K (Cellano) antigen was found in all children, and 93% of them had kk phenotype and 7% - Kk. The Сw (Willis) antigen was revealed only in 5 (6.0%) Rh(D)-positive patients with rare phenotypes - CwCceeK-, CwccEeK-, CwCcEEK-, CwCcEeK-. Matching a donor for patients with one of these phenotypes could pose a problem. Conclusions: Studying phenotypic characteristics of red blood cells is necessary for providing a successful blood transfusion, especially in children Kell-positive for the K antigen, in children homozygous for the C antigen with ССDееК- phenotype and in children with the Сw antigen and СwСсееК-, СwссЕеК-, СwСсЕЕК- and СwСсЕеК- phenotypes.

Molecular genotyping of Indian blood group system antigens in Indian blood donors

2018 ◽  
Vol 57 (3) ◽  
pp. 388-390
Author(s):  
Harita Gogri ◽  
Pranali Pitale ◽  
Manisha Madkaikar ◽  
Swati Kulkarni
Keyword(s):  
Blood Group ◽  
Blood Donors ◽  
Blood Group System ◽  
Molecular Genotyping ◽  
Group System ◽  
Indian Blood

scholarly journals Distribution of Blood Groups in Medical Students: A Comparative Study

2020 ◽  
Vol 8 (1) ◽  
pp. 42-46
Author(s):  
Bishal Joshi ◽  
Sanjit Kumar Kar ◽  
Shankar Yadav ◽  
Prem Kumar Yadav ◽  
Narayan Bahadur Mahotra ◽  
...  
Keyword(s):  
Medical Students ◽  
Red Blood Cells ◽  
Blood Group ◽  
Blood Cells ◽  
Blood Groups ◽  
Test Method ◽  
Fetal Life ◽  
Human Red Blood Cells ◽  
Slide Test ◽  
Group B

INTRODUCTION: Blood groups depend on antigens present on the surface of red blood cells. Scientists have discovered at least 30 common antigens and hundreds of rare antigens causing antigen-antibody reaction in human red blood cells. These antigens are genetically determined and are developed in fetal life and remain unchanged till death. Many blood group systems are identified but ABO and Rh blood groups are more antigenic. MATERIAL AND METHODS: In the present study, we observed ABO and Rh blood groups of 3057 students who studied in Universal College of Medical Sciences, Bhairahawa, Nepal from 1998 AD to 2019 AD, using open slide test method. The data was analyzed using SPSS version 20. RESULTS: Results  of the present study indicated that the most common blood group was O (36.8%)  followed by blood group B (31.1%) and blood group A (24.9%) and least common blood group was AB (7.2%) i.e. O>B>A>AB. The same sequence of ABO blood grouping was seen in both male and female.  Rh positive blood group was found in 95.4% and Rh negative blood group was found in 4.6% of population. CONCLUSION: Knowledge of distribution of blood group is very important for medical students as they can serve as immediate blood donor in emergency conditions. This study can provide insight to advanced studies in future which can relate blood groups with medical conditions.

Prevention of rhesus immunisation

1970 ◽  
Vol 8 (21) ◽  
pp. 81-83
Keyword(s):  
Blood Transfusion ◽  
Blood Group ◽  
Blood Cells ◽  
Antibody Formation ◽  
Subsequent Pregnancy ◽  
Abo Blood Group ◽  
Blood Group System ◽  
Maternal Circulation ◽  
Rh Immunisation ◽  
Negative Woman

If red blood cells possessing the D or Rho antigen (Rh) enter the circulation of an Rh-negative woman she may develop Rh antibodies. This is usually the result of pregnancy, but it may follow a mismatched blood transfusion. About one in six Rh-negative women who have been delivered of an Rh-positive infant are found to have antibodies during a subsequent pregnancy and abortion carries the same risk. Incompatibility of the mother with the fetus in the ABO blood group system tends to protect the mother from Rh immunisation, for the mother’s anti-A or anti-B antibody reacts with any fetal A or B Rh-positive cells which may pass into the maternal circulation, so virtually confining them to organs or tissues where antibody formation is unlikely.1

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