Ara h 1 and Ara h 6 Sensitization Causes Clinical Peanut Allergy in Ara h 2-Negative Individuals

2018 ◽  
Vol 178 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Helga Magnusdottir ◽  
Anna Gudrun Vidarsdóttir ◽  
Bjorn Runar Ludviksson ◽  
Michael Clausen ◽  
Sigrun Helga Lund ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Ara H 2 ◽  
Ara H 1 ◽  
Ara H 6

Ara h 2 and Ara h 6 sensitization predicts peanut allergy in Mediterranean pediatric patients

2014 ◽  
Vol 25 (7) ◽  
pp. 662-667 ◽  
Author(s):  
Chantal Agabriel ◽  
Ouafeh Ghazouani ◽  
Joëlle Birnbaum ◽  
Valérie Liabeuf ◽  
Françoise Porri ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Pediatric Patients ◽  
Ara H 2 ◽  
Ara H 6

Mixed antibody and T cell responses to peanut and the peanut allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6 in an oral sensitization model

2004 ◽  
Vol 34 (9) ◽  
pp. 1422-1428 ◽  
Author(s):  
F. van Wijk ◽  
S. Hartgring ◽  
S. J. Koppelman ◽  
R. Pieters ◽  
L. M. J. Knippels
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Ara H 2 ◽  
Cell Responses ◽  
Ara H 1 ◽  
Ara H 3 ◽  
Peanut Allergens ◽  
Ara H 6

scholarly journals Peanut components measured by ISAC: comparison with ImmunoCap and clinical relevance in peanut allergic children

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
H. K. Brand ◽  
M. W. J. Schreurs ◽  
J. A. M. Emons ◽  
R. Gerth van Wijk ◽  
H. de Groot ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Solid Phase ◽  
Area Under The Curve ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Golden Standard ◽  
Ara H 6 ◽  
Food Challenges ◽  
Double Blinded

Abstract Background Specific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy. Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard. Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0.906 and 0.902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r = 0.88; p < 0.01). Conclusion Ara h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy in Dutch children, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of different peanut components using ISAC is an advantage and clinically useful to detect peanut allergic children that are Ara h 2 negative but sensitized to other peanut proteins such as Ara h 6.

scholarly journals Oxidative Stability of Protease Treated Peanut with Reduced Allergenicity

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 762
Author(s):  
Jianmei Yu ◽  
Ivy N. Smith ◽  
Nadia Idris ◽  
Nicole Gregory ◽  
Nona Mikiashvili
Keyword(s):  
Oxidative Stability ◽  
Sandwich Elisa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Enzyme Treatment ◽  
Ara H 2 ◽  
Ara H 1 ◽  
Protease Treatment ◽  
Ara H 6 ◽  
The Impact

Oxidative stability and allergenicity are two major concerns of peanuts. This study evaluated the impact of protease treatment of peanuts on its oxidative stability during storage. The raw and dry-roasted peanut kernels were hydrolyzed with Alcalase solution at pH 7.5 for 3 h. The contents of Ara h 1, Ara h 2, and Ara h 6 in peanuts were determined before and after enzyme treatment by a sandwich ELISA. After drying, the samples were packed in eight amber glass jars and stored at 37 °C for 1–8 weeks. Controls are untreated raw and dry-roasted peanuts packed and stored in the same way as their treated counterparts. Samples were taken biweekly to determine peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) as indicators of oxidation (n = 3), and to determine antioxidant activity. Alcalase treatment reduced intact major allergens Ara h 1, Ara h 2, and Ara h 6 by 100%, 99.8%, and 85%, respectively. The PVs of Alcalase-treated raw and roasted peanuts was lower than those of untreated (p < 0.05) over the 8-week storage. The TBARS of Alcalase-treated raw peanuts were slightly higher than that of untreated (p < 0.05), but the TBARS of Alcalase-treated dry-roasted peanuts were slightly but significantly lower than that of untreated (p < 0.05). The protease treatment increased the antioxidant activities including reducing power, DPPH free radical scavenging capacity, and metal chelating capacity of peanuts.

scholarly journals Peanut Components Measured by ISAC: Comparison With ImmunoCap and Clinical Relevance in Peanut Allergic Children

2021 ◽  
Author(s):  
Kim Brand ◽  
Marco Schreurs ◽  
Joyce Emons ◽  
Roy Gerth van Wijk ◽  
Hans de Groot ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Solid Phase ◽  
Area Under The Curve ◽  
Specific Ige ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Golden Standard ◽  
Ara H 6 ◽  
Food Challenges ◽  
Double Blinded

Abstract BackgroundSpecific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy.Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard.Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0,906 and 0,902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r=0.88. p<0.01). ConclusionAra h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of Ara h 6 and Ara h 2 sIgE using ISAC is an advantage and clinically useful to detect peanut allergic children that are monosensitized to Ara h 6.

scholarly journals The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children

2017 ◽  
Vol 139 (1) ◽  
pp. 358-360.e8 ◽  
Author(s):  
Francine C. van Erp ◽  
Edward F. Knol ◽  
Bo Pontoppidan ◽  
Yolanda Meijer ◽  
Cornelis K. van der Ent ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Ara H 2 ◽  
Ara H 6

scholarly journals A phase 1 study of heat/phenol-killed,E. coli-encapsulated, recombinant modified peanut proteins Ara h 1, Ara h 2, and Ara h 3 (EMP-123) for the treatment of peanut allergy

Allergy ◽  
2013 ◽  
Vol 68 (6) ◽  
pp. 803-808 ◽  
Author(s):  
R. A. Wood ◽  
S. H. Sicherer ◽  
A. W. Burks ◽  
A. Grishin ◽  
A. K. Henning ◽  
...  
Keyword(s):  
Peanut Allergy ◽  
Phase 1 ◽  
Phase 1 Study ◽  
Ara H 2 ◽  
E Coli ◽  
Ara H 1 ◽  
Ara H 3 ◽  
Peanut Proteins
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