scholarly journals Protective Effects of Hydrogen Sulfide Against Cigarette Smoke Exposure-Induced Placental Oxidative Damage by Alleviating Redox Imbalance via Nrf2 Pathway in Rats

2018 ◽  
Vol 48 (5) ◽  
pp. 1815-1828 ◽  
Author(s):  
Fusheng Zhao ◽  
Fang Lei ◽  
Xiang Yan ◽  
Senfeng Zhang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Oxidative Damage ◽  
Cigarette Smoke ◽  
Health Hazard ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Related Factor ◽  
Protective Effects ◽  
Nrf2 Pathway ◽  
Redox Imbalance

Background/Aims: Cigarette smoke exposure (CSE) during pregnancy is a well-recognized health hazard that causes placental damage. Hydrogen sulfide (H2S) has been reported to protect multiple organs from injury. However, the protective effects of H2S have not been tested in the placenta. This study aimed to explore the potential of H2S in protecting placenta against oxidative injury induced by CSE during pregnancy and the possible underlying mechanisms. Methods: Pregnant SD rats were randomly divided into 4 groups: NaCl, NaHS (a donor of H2S), CSE and CSE+NaHS. Placental oxidative damage was detected by 8-hydroxy-2-deoxyguanosine (8-OHdG) stain and malondialdehyde (MDA) assay. Placental redox status was assessed by measuring reactive oxygen species (ROS), total antioxidant capacity (T-AOC) and glutathione (GSH) levels, as well as copper/zinc SOD (SOD1), manganese SOD (SOD2), catalase (CAT) and glutathione peroxidase (GPx) activities and expressions. Meanwhile, nuclear factor erythroid 2-related factor 2 (Nrf2) was analyzed by immunohistochemistry, real-time PCR and Western blot. Results: We found that NaHS markedly reduced the elevated levels of 8-OHdG and MDA induced by CSE. Further, NaHS treatment effectively mitigated CSE-induced placental redox imbalance by inhibiting ROS production, restoring T-AOC level, increasing GSH/GSSG ratio, and augmenting SOD1 SOD2, CAT and GPx activities and expressions. More notably, NaHS administration also reversed the aberrant decrease of Nrf2 due to CSE in rat placentas. Conclusion: Our data demonstrate that H2S can protect against CSE-induced placental oxidative damage probably by alleviating redox imbalance via Nrf2 pathway.

scholarly journals Acute cigarette smoke exposure induces oxidative damage and inflammation in Wistar rats: impact on lungs and erythrocytes

2021 ◽  
Vol 3 (1) ◽  
pp. 120-128
Author(s):  
Shahdat Hossain ◽  
Marzan Sarkar ◽  
Sujan Bhowmick ◽  
Jakir Hussain ◽  
Mahmudul Hasan ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure

scholarly journals Protective Effects of Quercetin on Livers from Mice Exposed to Long-Term Cigarette Smoke

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Pedro A. Machado-Junior ◽  
Natália P. S. Araújo ◽  
Ana B. F. Souza ◽  
Thalles F. Castro ◽  
Michel Oliveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Cigarette Smoke ◽  
Liver Diseases ◽  
Inflammatory Cells ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Protective Effects ◽  
Histopathological Changes

Cigarette smoke is highly toxic, and it can promote increased production of reactive species and inflammatory response and leads to liver diseases. Quercetin is a flavonoid that displays antioxidant and anti-inflammatory activities in liver diseases. This study aimed at evaluating the protective effects of quercetin on livers from mice exposed to long-term cigarette smoke exposure. Male C57BL/6 mice were divided into five groups: control (CG), vehicle (VG), quercetin (QG), cigarette smoke (CSG), quercetin, and cigarette smoke (QCSG). CSG and QCSG were exposed to cigarette smoke for sixty consecutive days; at the end of the exposures, all animals were euthanized. Mice that received quercetin daily and were exposed to cigarette smoke showed a reduced influx of inflammatory cells, oxidative stress, inflammatory reaction, and histopathological changes in the liver, compared to CSG. These results suggest that quercetin may be an effective adjuvant for treating damage to the liver due to cigarette smoke exposure.

Effect of vitamin E and selenium on antioxidant enzymes in brain, kidney and liver of cigarette smoke-exposed mice

Biologia ◽  
2007 ◽  
Vol 62 (3) ◽  
Author(s):  
Aysun Ozkan ◽  
Kayahan Fiskin ◽  
Ayse Ayhan
Keyword(s):  
Vitamin E ◽  
Oxidative Damage ◽  
Cigarette Smoke ◽  
Enzyme Activities ◽  
Glutathione Transferase ◽  
Cigarette Smoke Exposure ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Enzymes Activities ◽  
Combined Application

AbstractThe present study was conducted to evaluate the protective effects of vitamin E and selenium (Se) application on alteration of antioxidant enzyme activities against cigarette smoking induced oxidative damage in brains, kidneys and liver of mice. Male mice (balb/c) were exposed to cigarette smoke and treated with Se and/or vitamin E. Glutathione transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GRX), superoxide dismutase (SOD) and catalase (CAT) enzyme activities in mice brain, kidney and liver were measured spectrophotometrically. GST, GPX, GRX, SOD and CAT enzyme activities in the brains of smoke-exposed mice were found lower than the enzymes activities of control mice and Se-and vitamin E-treated mice at the end of the three and five months. Opposite to brain, enzyme activities in kidneys and livers of smoke-exposed mice were found higher than the enzymes activities of control mice and Se-and vitamin E-treated mice at the end of the three and five months. Activities of GST, GPX, GRX SOD and CAT in the livers, kidneys and brains of smoke-exposed mice were found statistically different (p < 0.01) compared to control mice and Se-and vitamin E-treated mice. Combined application of vitamin E and Se had an additive protective effect against changing enzymes activities in smoke-exposed mice livers, kidneys and brains at the end of the both application periods. These results suggest that cigarette smoke exposure enhances the oxidative stress, thereby disturbing the tissue antioxidant defense system and combined application of vitamin E and Se protects the brain, kidney and liver from oxidative damage through their antioxidant potential.

Hydrogen sulfide alleviates placental injury induced by maternal cigarette smoke exposure during pregnancy in rats

Nitric Oxide ◽  
2018 ◽  
Vol 74 ◽  
pp. 102-111 ◽  
Author(s):  
Fusheng Zhao ◽  
Fang Lei ◽  
Senfeng Zhang ◽  
Xiang Yan ◽  
Wen Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Cigarette Smoke ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Exposure During Pregnancy
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