scholarly journals Antispasmodic Effect of Blackcurrant (Ribes nigrum L.) Juice and Its Potential Use as Functional Food in Gastrointestinal Disorders

2018 ◽  
Vol 27 (2) ◽  
pp. 179-185 ◽  
Author(s):  
Bojana Miladinovic ◽  
Suzana  Brankovic ◽  
Milica Kostic ◽  
Milica Milutinovic ◽  
Nemanja Kitic ◽  
...  
Keyword(s):  
Functional Food ◽  
Statistical Significance ◽  
Barium Chloride ◽  
Gastrointestinal Disorders ◽  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Antispasmodic Effect ◽  
Spontaneous Contractions ◽  
Isolated Rat

Objective: The purpose of this study was to investigate the relaxative effects of blackcurrant juice on the gastrointestinal smooth muscle in vitro. Materials and Methods: Berries of the blackcurrant cultivar Ometa were used for the preparation of the juice used. The spasmolytic activity of blackcurrant juice was tested on rat ileum isolated from male Wistar rats by monitoring its influence on spontaneous contractions, as well as contractions induced by potassium chloride (KCl), barium chloride (BaCl2), calcium chloride (CaCl2), and acetylcholine (Ach). The results are expressed as the mean ± standard deviation obtained in 6 measurements and statistical significance was determined by the Student t test, with p < 0.05 taken as significant. Results: The blackcurrant cultivar Ometa significantly reduced the frequency and the amplitude of spontaneous contractions (57.94 ± 3.44%) and Ach-induced contractions (42.74 ± 5.36%; p < 0.05) of the isolated rat ileum. Cumulative concentrations (0.01–3 mg/mL) of the Ometa juice also reduced contractions of the isolated rat ileum stimulated by KCl (51.46 ± 6.87%), CaCl2 (57.54 ± 6.47%), and BaCl2 (58.54 ± 10.55%). The inhibitory effects of the juice were proportional to the applied concentration. Conclusion: The antispasmodic effect of Ometa cultivar shows that common gastrointestinal disorders could be treated by the functional food.

Comparative spasmolytic effect between Cinnamomum tamala and Cinnamomum verum leaf essential oils and eugenol through in vitro and in silico approaches

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Anubhav Chakraborty ◽  
Vishwam Sankaran ◽  
Ramar Murugan ◽  
David Raj Chellappan
Keyword(s):  
Essential Oils ◽  
In Silico ◽  
Pharmacological Action ◽  
Lower Content ◽  
Nicotinic Acetylcholine ◽  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Cinnamomum Tamala ◽  
Isolated Rat

Abstract Cinnamomum tamala and Cinnamomum verum are known for their folk medicinal usage in treating gastrointestinal ailments. The spasmolytic activity of essential oils was studied using isolated rat ileum. The results indicate that C. tamala, despite having a lower content of eugenol (60%), shows a spasmolytic potential of 68.01 ± 2.63% (EC50 = 110.12 ± 13.58 μg/mL) while C. verum with rich eugenol (80%) shows lesser spasmolytic potential (38.96 ± 0.63%) and fails to attain an EC50 value. Upon comparison with standard eugenol’s percentage of spasmolytic (35.68 ± 2.57%), it is evident that the action of these essential oils does not solely rely on the major component but the synergistic role in association with other components of the mixture influences the pharmacological action of the essential oils. In silico docking of phytochemicals of leaf essential oils with M2 (M2AChR) and M3 muscarinic (M3AChR) and nicotinic acetylcholine receptor (nAChR) was carried out to determine the type of receptors through which the essential oils had spasmolytic potential. The binding affinity for eugenol with nAChR shows a better docking score than M2AChR and M3AChR.

Changes in spontaneous contractions of rat ileum by aflatoxin in vitro

2008 ◽  
Vol 46 (6) ◽  
pp. 2124-2127 ◽  
Author(s):  
Nevcihan Gursoy ◽  
Bulent Sarac ◽  
Nedim Durmus ◽  
Ahmet Parlak ◽  
Sahin Yildirim ◽  
...  
Keyword(s):  
Rat Ileum ◽  
Spontaneous Contractions

scholarly journals Spasmolytic Activity of the Ethanol Extract of Sideritis raeseri spp. raeseri Boiss. & Heldr. on the Isolated Rat Ileum Contractions

2011 ◽  
Vol 14 (5) ◽  
pp. 495-498 ◽  
Author(s):  
Suzana Brankovic ◽  
Dusanka Kitic ◽  
Mirjana Radenkovic ◽  
Slavimir Veljkovic ◽  
Teodora Jankovic ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Isolated Rat

scholarly journals Effects of Funchicórea®, a Traditional Brazilian Herbal Complex, on Intestinal Motility in Healthy and Constipated Rodents

2020 ◽  
pp. 26-36
Author(s):  
Valéria L. Assis ◽  
Ana C. M. F. Veras ◽  
Priscilla M. P. Maciel ◽  
José G. F. Albuquerque ◽  
Carmem Zancanella ◽  
...  
Keyword(s):  
Scientific Data ◽  
Intestinal Transit ◽  
Female Rats ◽  
Control Group ◽  
Male Mice ◽  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Male And Female Rats

Ethnopharmacological Relevance: The traditional herbal medicinal product Funchicórea® has been widely used in clinical practice for the treatment of intestinal colic and constipation in newborns. However, no scientific data on the herbal product to prove its efficacy is available. Aim of the Study: This study aimed to evaluate the laxative and spasmolytic actions of Funchicórea®. Materials and Methods: Wistar rats (Rattus norvegicus) and Swiss mice (Mus musculus) of both sex, were used. In vivo pharmacological assays were performed to evaluate the stimulating effect on the gastrointestinal tract, and in vitro studies to verify its spasmodic activity. Results: Funchicórea® increased the motility of the small intestine in male mice at doses of 100 mg/kg (161.66±14.86 %, n=6) and 200 mg/kg (151.04±17.17 %, n=6) compared to control (100.00±10.49 %, n=6). However, this drug did not induce any change in intestinal transit in female mice. The intestinal transit of male mice treated with loperamide (3 mg/kg/day, during three days) was reduced 66.25±7.49 % (n=8) compared to the control group (100.00±5.16%, n=8) and we observed the normalization of the intestinal transit in constipated animals treated with Funchicórea® 100 mg/Kg (98.42±6.33 %, and 200 mg/kg (99.32±8.47%, n=7). Similar results were observed in the quantification for 24 hours of male and female rats faeces constipated by loperamide (3 mg/kg/day three days), however, in both animals groups treated with Funchicórea® 100 mg/kg (1.24±2.90 g, male; 3.60±0.80 g, female, n=6) and 200 mg/Kg (8.70±2.01 g, male, 10.03±1.30 g, female, n=6) the levels of faeces returned to basal values compared to constipated group (4.01±1.43 g, male; 1.70±0.10 g, female, n=6). In addition, Funchicórea® (0.01-1000 μg/mL) elicited relaxation in rat ileum pre-contracted by KCl 40 mM (Emax=97.5±7.0 %, n=7) and carbachol (1 μM, Emax=100.0±7.0 %, n=7). Conclusion: The results obtained demonstrated that the herbal medicine Funchicórea® acts by stimulating the intestine of rats and mice and has spasmolytic activity in isolated rat ileum.

scholarly journals The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility

2018 ◽  
Vol 75 (8) ◽  
pp. 809-814 ◽  
Author(s):  
Marija Gocmanac-Ignjatovic ◽  
Dusanka Kitic ◽  
Mirjana Radenkovic ◽  
Milica Kostic ◽  
Milica Milutinovic ◽  
...  
Keyword(s):  
Dependent Manner ◽  
Foeniculum Vulgare ◽  
Concentration Dependent Manner ◽  
Rat Ileum ◽  
Caffeoylquinic Acid ◽  
Dicaffeoylquinic Acid ◽  
Stem Extract ◽  
Spontaneous Contractions ◽  
Foeniculum Vulgare Miller ◽  
Isolated Rat

Background/Aim. The fennel (Foeniculum vulgare Miller, Apiaceae) has a long history of use as traditional herb medicine due to its carminative properties. The study was aimed to investigate the effects of aqueous and methanol fennel stem extracts on intestinal activity. Methods. Relaxant activity of aqueous and methanol fennel stem extracts was evaluated in vitro in three experimental models: spontaneous contraction, acetylcholine and potassium chloride (KCl)-induced contraction of an isolated rat ileum. The composition of aqueous and methanol fennel stem extracts was qualitatively analyzed using the high performance liquid chromatographic (HPLC) analysis. Results. In the presence of an aqueous fennel stem extract at a concentration of 3 mg/mL, the inhibition of the spontaneous contractions of isolated rat ileum was 35.05% ? 3.57%. In presence of a methanol fennel stem extract at the same concentration, the maximum reduction of the spontaneous contractions was 48.91% ? 6.31%. The extracts in a concentration- dependent manner significantly inhibited the acetylcholine and KCl induced contractions of the isolated rat ileum (p < 0.01). The following components were identified in fennel methanol stem extract: 3-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, rutin, miquelianin, quercetin heterosides, 1,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, apigenin and rosmarinic acid. In an aqueous extract, their presence is found in trace amounts. Conclusion. The results of this study showed that the aqueous and methanol fennel stem extracts have spasmolytic effects on the intestinal smooth muscle and may be used for the control of intestinal motility.

scholarly journals SPASMOLYTIC ACTIVITY OF THE AQUEOUS AND ETHANOL CELERY LEAVES (APIUM GRAVEOLENS L.) EXTRACTS ON THE CONTRACTION OF ISOLATED RAT ILEUM

2015 ◽  
pp. 11-16 ◽  
Author(s):  
Suzana Branković ◽  
Marija Gočmanac-Ignjatović ◽  
Milica Kostić ◽  
Milica Veljković ◽  
Bojana Miladinović ◽  
...  
Keyword(s):  
Apium Graveolens ◽  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Isolated Rat

Spasmolytic activity of Phagnalon saxatile (L.) Cass. on the isolated rat ileum

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
G Aviello ◽  
D Rigano ◽  
C Formisano ◽  
R Capasso ◽  
AA Izzo ◽  
...  
Keyword(s):  
Spasmolytic Activity ◽  
Rat Ileum ◽  
Isolated Rat

HEMMUNG DER UTERUSWIRKUNG VON OXYTOCIN DURCH WASSERLÖSLICHE STEROIDE

1965 ◽  
Vol 48 (4) ◽  
pp. 656-663 ◽  
Author(s):  
R. Hempel ◽  
F. Neumann
Keyword(s):  
Barium Chloride ◽  
Water Soluble ◽  
Rat Uterus ◽  
Per Se ◽  
Isolated Rat ◽  
Spontaneous Rhythm

ABSTRACT Various water-soluble steroids were tested in vitro on the isolated rat uterus for their oxytocin-antagonistic activities. Using progestional agents (19-nor-17α-hydroxyprogesterone-17-hemisulfate-sodium, Δ1,6-17α-hydroxyprogesterone-17-hemisulfate-sodium) oestrogens (oestrone-hemisulfate-sodium), and corticoids (dexamethasone-21-hemisulfate-sodium) it was possible to suppress both the oxytocin-induced single contraction and the spontaneous rhythm intensified by oxytocin. Except for the Δ1,6-17α-hydroxyprogesterone-sulfate-sodium, these compounds were unable to inhibit the single contractions of the uterus induced by serotonin, bradykinin and barium chloride. Following the administration of a mixture of the different watersoluble steroids an additional effect was observed. Based on these results, it can be assumed that the oxytocinantagonistic effect represents an activity per se which is peculiar to certain steroid structures.

SENSITIVITY OF THE RAT DIAPHRAGM TO GROWTH HORMONE.

1967 ◽  
Vol 54 (4) ◽  
pp. 645-662 ◽  
Author(s):  
Å. Hjalmarson ◽  
K. Ahrén
Keyword(s):  
Growth Hormone ◽  
Inhibitory Effect ◽  
Rat Diaphragm ◽  
Intracellular Accumulation ◽  
Slight Effect ◽  
Kidney Capsule ◽  
Muscle Tissues ◽  
Hypophysectomized Rats ◽  
Isolated Rat

ABSTRACT The effect of growth hormone (GH) in vitro on the rate of intracellular accumulation of the non-utilizable amino acid α-aminoisobutyric acid (AIB) was studied in the intact rat diaphragm preparation. Bovine or ovine GH (25 μg/ml incubation medium) markedly stimulated the accumulation of AIB-14C by diaphragms from hypophysectomized rats, while there was no or only a very slight effect on diaphragms from normal rats. In diaphragms from rats with the pituitary gland autotransplanted to the kidney capsule GH in vitro stimulated the accumulation of AIB-14C significantly more than in diaphragms from normal rats but significantly less than in diaphragms from hypophysectomized rats. Injections of GH intramuscularly for 4 days to hypophysectomized rats made the diaphragms from these rats less sensitive or completely insensitive to GH in vitro. These results indicate strongly that the relative insensitivity to GH in vitro of diaphragms from normal rats is due to the fact that the muscle tissues from these rats has been exposed to the endogenously secreted GH. The results show that GH can influence the accumulation of AIB-14C in the isolated rat diaphragm in two different ways giving an acute or »stimulatory« effect and a late or »inhibitory« effect, and that it seems to be a time-relationship between these two effects of the hormone.

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