scholarly journals Micro Integral Membrane Protein (MIMP), a Newly Discovered Anti-Inflammatory Protein of Lactobacillus Plantarum, Enhances the Gut Barrier and Modulates Microbiota and Inflammatory Cytokines

2018 ◽  
Vol 45 (2) ◽  
pp. 474-490 ◽  
Author(s):  
Mingming Yin ◽  
Xuebing Yan ◽  
Wenhao Weng ◽  
Yongzhi Yang ◽  
Renyuan Gao ◽  
...  
Keyword(s):  
Treatment Group ◽  
Membrane Protein ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Inflammatory Cytokines ◽  
Integral Membrane Protein ◽  
Gut Barrier ◽  
Culture Model ◽  
Anti Inflammatory ◽  
Colitis Model

Background/Aims: Recent studies have demonstrated that the manipulation of the gut microbiome represents a promising treatment for inflammatory bowel disease (IBD). We previously identified micro integral membrane protein (MIMP) as the smallest domain of surface layer protein from Lactobacillus Plantarum. However, the therapeutic relevance of MIMP in IBD remains unknown. Methods: We initially employed a dextran sodium sulphate (DSS)-induced colitis model and evaluated the effect of MIMP on the inflammation response, intestinal barrier and gut microbiota using histological examination, Fluorescein isothiocyanate-Dextran detection and pyrosequencing analysis respectively. We then established peripheral blood mononuclear cells (PBMCs) and an epithelial CaCO-2 co-culture model to investigate the regulatory role of MIMP in inflammatory cytokines. The level changes of inflammatory cytokines were detected using Enzyme-linked immunosorbent and real-time polymerase chain reaction assay. The involved regulatory mechanisms were investigated mainly using dual luciferase reporter and chromatin immunoprecipitation assay. Results: In the DSS-induced colitis model, we observed that MIMP intervention effectively improved the body weight loss, increased the colon length and decreased disease activity index. Consistently, the inflammation scores in the MIMP treatment group were significantly lower than those in the DSS treatment group. Furthermore, MIMP intervention was found to successfully neutralize DSS treatment by decreasing the expression of pro-inflammatory cytokines (IFN-γ, IL-17 and IL-23) and increasing the expression of anti-inflammatory cytokines (IL-4 and IL-10). Notably, the permeability assay demonstrated that the MIMP treatment group was remarkably lower than that in the DSS treatment group. We also showed that MIMP improved gut microbiota dysbiosis caused by DSS-induced inflammation. Additionally, in PBMCs and the CaCO-2 co-culture model, MIMP showed an obvious suppressive effect on lipopolysaccharide-induced inflammation in a time- and dose-dependent manner. Furthermore, we revealed that MIMP could modulate inflammatory cytokine expression through the toll-like receptor 4 pathway and histone acetylation. Conclusions: Our results suggested that MIMP showed a significant anti-inflammatory effect through regulating the gut barrier, microbiota and inflammatory cytokines. MIMP may have translational relevance as clinically relevant therapy for IBD patients.

scholarly journals Modulation of Pro-inflammatory and Anti-inflammatory Cytokines in the Fat by an Aloe Gel-based Formula, QDMC, Is Correlated with Altered Gut Microbiota

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Jinho An ◽  
Heetae Lee ◽  
Sungwon Lee ◽  
Youngcheon Song ◽  
Jiyeon Kim ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Inflammatory Cytokines ◽  
Anti Inflammatory ◽  
Aloe Gel

scholarly journals Alleviative Effects of Exopolysaccharide Produced by Lactobacillus helveticus KLDS1.8701 on Dextran Sulfate Sodium-Induced Colitis in Mice

2021 ◽  
Vol 9 (10) ◽  
pp. 2086
Author(s):  
Yin Liu ◽  
Shujuan Zheng ◽  
Jiale Cui ◽  
Tingting Guo ◽  
Jingtao Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Inflammatory Cytokines ◽  
Intestinal Inflammation ◽  
Activity Index ◽  
Lactobacillus Helveticus ◽  
Mucosal Barrier ◽  
Microbiota Composition ◽  
Mucosal Barrier Function ◽  
Anti Inflammatory ◽  
Gut Microbiota Composition

Ulcerative colitis (UC) is a non-specific chronic inflammatory disease with lesions located in the colon and rectum. The aim of this study was to evaluate the anti-inflammatory effects of exopolysaccharide-1 (EPS-1) isolated by L. helveticus KLDS1.8701 on UC. The anti-inflammatory effects of EPS-1 were studied using dextran sulphate sodium (DSS)-induced UC model. In vivo results showed that EPS-1 administration significantly ameliorated weight loss, colon shortening, disease activity index (DAI) score, myeloperoxidase (MPO) activity, and colon tissue damage. In addition, EPS-1 administration significantly decreased the levels of pro-inflammatory cytokines and increased levels of anti-inflammatory cytokines. Meanwhile, EPS-1 administration significantly up-regulated the expression of tight junction proteins and mucin. Furthermore, EPS-1 administration modulated gut microbiota composition caused by DSS and increased the short-chain fatty acids (SCFAs) levels. Collectively, our study showed the alleviative effects of EPS- isolated by L. helveticus KLDS1.8701 on DSS-induced UC via alleviating intestinal inflammation, improving mucosal barrier function, and modulating gut microbiota composition.

Millet shell polyphenols prevent atherosclerosis by protecting gut barrier and remodeling gut microbiota in ApoE-/- mice

2021 ◽  
Author(s):  
Fengming Liu ◽  
Shuhua Shan ◽  
Hanqing Li ◽  
Jiangying Shi ◽  
Ruilin Hao ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Gut Microbiota ◽  
Inflammatory Disease ◽  
Chronic Inflammatory Disease ◽  
Gut Barrier ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Atherosclerosis, the major cause of cardiovascular disease, is a chronic inflammatory disease. The anti-inflammatory effect of certain polyphenols have been recognized. Active polyphenols were extracted from millet shell (MSP)，and the...

scholarly journals Effect of Extracellular Vesicles Derived From Lactobacillus plantarum Q7 on Gut Microbiota and Ulcerative Colitis in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Haining Hao ◽  
Xinyi Zhang ◽  
Lingjun Tong ◽  
Qiqi Liu ◽  
Xi Liang ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Extracellular Vesicles ◽  
Intestinal Inflammation ◽  
Body Weight Loss ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Probiotics plays an important role in regulating gut microbiota and maintaining intestinal homeostasis. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect. However, the anti-inflammatory effect and mechanism of probiotics derived EVs on inflammatory bowel disease (IBD) remains unclear. In this study, the effect of Lactobacillus plantarum Q7-derived extracellular vesicles (Q7-EVs) on gut microbiota and intestinal inflammation was investigated in C57BL/6J mice. The results showed that Q7-EVs alleviated DSS-induced colitis symptoms, including colon shortening, bleeding, and body weight loss. Consumption of Q7-EVs reduced the degree of histological damage. DSS-upregulated proinflammatory cytokine levels including IL-6, IL-1β, IL-2 and TNF-α were reduced significantly by Q7-EVs (p < 0.05). 16S rRNA sequencing results showed that Q7-EVs improved the dysregulation of gut microbiota and promoted the diversity of gut microbiota. It was observed that the pro-inflammatory bacteria (Proteobacteria) were reduced and the anti-inflammatory bacteria (Bifidobacteria and Muribaculaceae) were increased. These findings indicated that Q7-EVs might alleviate DSS-induced ulcerative colitis by regulating the gut microbiota.

scholarly journals Corrigendum: The new views on the state of the gut microbiota in obesity and diabetes mellitus type 2 (Diabetes mellitus. 2019;22(3). doi: 10.14341/DM10194)

2019 ◽  
Vol 22 (4) ◽  
pp. 403-404
Author(s):  
Elena V. Pokrovskaya ◽  
Minara S. Shamkhalova ◽  
Marina V. Shestakova
Keyword(s):  
Diabetes Mellitus ◽  
Gut Microbiota ◽  
Inflammatory Cytokines ◽  
Diabetes Mellitus Type 2 ◽  
Interleukin 1 ◽  
The State ◽  
Diabetes Mellitus Type ◽  
Obesity And Diabetes ◽  
Anti Inflammatory

A corrigendum on The new views on the state of the gut microbiota in obesity and diabetes mellitus type 2 by Elena V. Pokrovskaya, Minara S. Shamkhalova, Marina V. Shestakova (2019). Diabetes Mellitus. 22(3). doi: 10.14341/DM10194There is an error on the page 255: "Moreover, in obese patients, the concentration of circulating LPS increases by 20%, and in patients with diabetes mellitus, it increases by 125%. LPS is transported from cells of the large intestine into the bloodstream through chylomicrons or through intercellular gaps in the intestinal wall; by forming a complex of CD14 with Toll-like receptor 4 of macrophages and endothelial cells, it causes the release of anti-inflammatory cytokines: namely, interleukin-1, interleukin-6 and tumour necrosis factor alpha ". Instead of " pro-inflammatory cytokines" was published " anti-inflammatory cytokines ".Literary source Dahiya DK, Renuka, Puniya M, et al. Gut Microbiota Modulation and Its Relationship with Obesity Using Prebiotic Fibers and Probiotics: A Review. Front Microbiol. 2017;8:563. doi: https://doi.org/10.3389/fmicb.2017.00563 is listed twice (№№ 7 and 13) in the list of references.The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way.The original article has been updated.

scholarly journals Probiotic Characteristics and Anti-inflammatory Properties of an Indian Indigenous Lactobacillus plantarum against Pathogenic Salmonella Typhimurium

2021 ◽  
Author(s):  
Projoyita Samanta ◽  
V Deepak Bamola ◽  
Bimal Das ◽  
Parthoprasad Chattopadhyay ◽  
Rama Chaudhry
Keyword(s):  
Salmonella Typhimurium ◽  
Lactobacillus Plantarum ◽  
Inflammatory Cytokines ◽  
Good Adhesion ◽  
Huge Number ◽  
Therapeutic Application ◽  
Ncbi Accession ◽  
Igg Receptors ◽  
Antagonistic Potential ◽  
Anti Inflammatory

Probiotic should be well characterized for any preventive and therapeutic application. Studies have reported that the population specific indigenous probiotics are more effective. Huge number of probiotics are available globally but indigenous Indian probiotic candidates are limited. Therefore, a study was conducted to evaluate probiotic characteristics and anti-inflammatory properties of an Indian indigenous Lactobacillus plantarum (Lp1) – (NCBI accession no- MN386242) against gastrointestinal (GI) pathogen Salmonella Typhimurium (ST) using Caco-2 cell lines. Anti-inflammatory properties were assessed by evaluating the expression of Immunoglobulins (IgA, IgG) receptors, Toll Like Receptors (TLR2, TLR4) and cytokine (IL10, IL-8, IL17 and IL23) using flowcytometry and Real Time-PCR. Lp1 showed good adhesion and ability to survive in high acidic and bile conditions of the gut. Lp1 demonstrated antagonistic potential against GI pathogens and modulated pro and anti-inflammatory cytokines expression significantly. Observed result indicated that this indigenous Lp1 is a good probiotic candidate with anti-inflammatory potential and can be evaluated further clinically for the treatment or prevention of salmonellosis and other GI inflammatory disorders.

Gut microbiota modulation and anti-inflammatory properties of mixed lactobacilli in dextran sodium sulfate-induced colitis in mice

2021 ◽  
Author(s):  
Jialu Shi ◽  
Qing gang Xie ◽  
Yingxue Yue ◽  
Qingxue Chen ◽  
Lina Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Sodium Sulfate ◽  
Dextran Sodium Sulfate ◽  
Lactobacillus Helveticus ◽  
Immunomodulatory Activity ◽  
Anti Inflammatory

Lactobacillus acidophilus KDSL 1.0901，Lactobacillus helveticus KDSL 1.8701and Lactobacillus plantarum KDSL 1.0318 showed antibacterial activity, antioxidant activity and immunomodulatory activity. In the current study, we evaluated the effects of three tested...

scholarly journals The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4149
Author(s):  
Juan Zhang ◽  
Li Cao ◽  
Yu Sun ◽  
De-Gang Qing ◽  
Xiao-Qin Xu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Epithelium ◽  
Intestinal Barrier ◽  
High Dose ◽  
Gut Microflora ◽  
Gut Barrier ◽  
16S Rrna Analysis ◽  
Licochalcone A ◽  
Anti Inflammatory ◽  
Regulatory Effects

The gut epithelium is a mechanical barrier that protects the host from the luminal microenvironment and interacts with the gut microflora, which influences the development and progression of ulcerative colitis (UC). Licochalcone A (LA) exerts anti-inflammatory effects against UC; however, whether it also regulates both the gut barrier and microbiota during colitis is unknown. The current study was conducted to reveal the regulatory effects of LA on the intestinal epithelium and gut microflora in C57BL/6 mice subjected to dextran sodium sulfate (DSS). Sulfasalazine (SASP) was used as the positive control. Results of clinical symptoms evaluation, hematoxylin, and eosin (H&E) staining, and enzyme-linked immunosorbent (ELISA) assays showed that LA significantly inhibited DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and gut inflammation. Additionally, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunohistochemical (IHC) analysis showed that LA maintained the integrity of the intestinal barrier by suppressing cell apoptosis and preserving the expression of tight junction (TJ) proteins. Notably, the optimal dose of LA for gut barrier preservation was low, while that for anti-inflammatory effects was high, indicating that LA might preserve gut barrier integrity via direct effects on the epithelial cells (ECs) and TJ proteins. Furthermore, 16S rRNA analysis suggested that the regulatory effect of LA on the gut microbiota differed distinctly according to dose. Correlation analysis indicated that a low dose of LA significantly modulated the intestinal barrier-associated bacteria as compared with a moderate or high dose of LA. Western blot (WB) analysis indicated that LA exhibited anti-UC activity partly by blocking the mitogen-activated protein kinase (MAPK) pathway. Our results further elucidate the pharmacological activity of LA against UC and will provide valuable information for future studies regarding on the regulatory effects of LA on enteric diseases.

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