Fluoride Levels in Unstimulated Whole Saliva following Clinical Application of Different 5% NaF Varnishes

2018 ◽  
Vol 52 (6) ◽  
pp. 431-438
Author(s):  
Danika Downey ◽  
Joseph Dennison ◽  
George J. Eckert ◽  
Susan E. Flannagan ◽  
Gisele F. Neiva ◽  
...  
Keyword(s):  
Fluoride Concentration ◽  
Exclusion Criteria ◽  
Significance Level ◽  
Linear Mixed Effects ◽  
Institutional Review Board Approval ◽  
Institutional Review ◽  
Whole Saliva ◽  
Unstimulated Whole Saliva ◽  
Time Point

The aim of this study was to evaluate the fluoride release from differently formulated 5% NaF varnishes into unstimulated whole saliva in vivo. The fluoride concentration in unstimulated whole saliva was determined after the application of 3 different 5% NaF varnishes (5% NaF, 5% NaF + tricalcium phosphate [TCP], and 5% NaF + amorphous calcium phosphate [ACP]) or a placebo. Fifteen subjects were recruited and enrolled following Institutional Review Board approval based upon the inclusion/exclusion criteria of this study. A cross-over study design was used for the application of either one of the 5% NaF varnishes or a placebo. Unstimulated whole saliva was collected at baseline and at 1, 4, 6, 26, and 50 h following application and analyzed for supernatant ionic fluoride and whole fluoride by microdiffusion. Linear mixed-effects models (5% significance level) were used to determine the effects of varnish and time on the salivary fluoride concentration. The highest amount of fluoride in saliva was found 1 h after application of the fluoride varnishes, with no significant differences among the treatment varnishes with respect to whole fluoride but with lower levels for 5% NaF + ACP in the saliva supernatant. Salivary fluoride levels at 4, 6, and 26 h decreased at each time point and were generally significantly higher for 5% NaF and 5% NaF + TCP. After 50 h, fluoride levels in saliva for all groups were at or below baseline levels. In conclusion, the formulation of other ingredients in fluoride varnishes can affect the fluoride concentration in saliva. The reasons for this phenomenon warrant further investigation since it might affect efficacy of the treatment. This trial is registered at ClinicalTrials.gov (NCT01629290).

Fluoride in Dental Biofilm Varies across Intra-Oral Regions

2017 ◽  
Vol 51 (4) ◽  
pp. 402-409 ◽  
Author(s):  
Line Staun Larsen ◽  
Vibeke Baelum ◽  
Livia Maria Andaló Tenuta ◽  
Alan Richards ◽  
Bente Nyvad
Keyword(s):  
Sampling Site ◽  
Fluoride Concentration ◽  
Cross Sectional Study ◽  
Anterior Region ◽  
Cross Sectional ◽  
Dental Biofilm ◽  
Whole Saliva ◽  
Biofilm Fluid ◽  
Fluoride Distribution ◽  
Unstimulated Whole Saliva

Information on differences in biofilm fluoride concentration across intra-oral regions may help explain the distribution of caries within the dentition. The aim of this cross-sectional study was to describe the fluoride concentration in saliva and in biofilm fluid and biofilm solids across 6 intra-oral regions. Unstimulated whole saliva was collected from 42 participants and biofilm harvested from the buccal sites in the 4 molar and 2 anterior regions. Samples were collected at least 1 h after use of fluoride dentifrice. No attempt was made to control the participants' food consumption or use of other topical agents. Centrifuged saliva, biofilm fluid, and biofilm solids were analysed for fluoride using a fluoride ion-selective electrode, adapted for microanalysis. Fluoride in biofilm varied across intra-oral regions. The mean biofilm fluid fluoride concentrations across the oral cavity ranged from 11.6 to 16.8 µM, being statistically significantly higher in the upper anterior region than in any other region. In all regions the fluoride concentration in biofilm fluid was higher than in saliva. For biofilm solids the fluoride concentration was highest in the lower anterior region (2,461 μmol/kg) and lowest in the lower molar regions (388 and 406 μmol/kg, respectively). Within biofilm, the solids contained most of the fluoride (81 to >99%). The biofilm fluid fluoride concentration was significantly positively associated with salivary fluoride and only marginally associated with that of biofilm solids. In conclusion, this study has shown pronounced differences in fluoride distribution across intra-oral regions and compartments. This shows that the sampling site is a crucial factor for studies of biofilm fluoride.

Detection and quantification of monomers in unstimulated whole saliva after treatment with resin-based composite fillings in vivo

2012 ◽  
Vol 120 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Vibeke B. Michelsen ◽  
Hilde B. M. Kopperud ◽  
Gunvor B. Lygre ◽  
Lars Björkman ◽  
Einar Jensen ◽  
...  
Keyword(s):  
Whole Saliva ◽  
Unstimulated Whole Saliva ◽  
Detection And Quantification

Methotrexate Elimination When Coadministered With Levetiracetam

2016 ◽  
Vol 50 (12) ◽  
pp. 1016-1022 ◽  
Author(s):  
David Reeves ◽  
Sarah DiDominick ◽  
Suzanne Finn ◽  
Hyeon Jin Kim ◽  
Amanda Shake
Keyword(s):  
Significant Interaction ◽  
Cox Regression ◽  
Potential Interaction ◽  
High Dose ◽  
Additional Risk ◽  
Institutional Review Board Approval ◽  
Institutional Review ◽  
Methotrexate Concentration ◽  
Clinically Significant ◽  
Time Point

Background: Delayed elimination of methotrexate was previously reported in 2 patients receiving concomitant levetiracetam. Objective: To explore the potential interaction between methotrexate and levetiracetam in patients receiving high-dose methotrexate. Methods: This retrospective study reviewed the records of 81 adults receiving 280 cycles of methotrexate to determine the effects of levetiracetam on methotrexate elimination. Institutional review board approval was obtained. Results: Levetiracetam was administered in 33 (12%) cycles of methotrexate. Patients receiving levetiracetam had significantly lower 24-hour methotrexate concentrations compared with those not receiving levetiracetam (2.91 vs 7.37 µmol/L, P = 0.005). Despite this difference, concentrations at 48 and 72 hours were similar between groups. Times to nontoxic methotrexate concentration (<0.1 µmol/L) were the same regardless of the presence of levetiracetam. The frequency of delayed elimination at 24, 48, and 72 hours was similar in both groups as was the frequency of delayed elimination at any time point. Cox regression demonstrated that levetiracetam was not a significant predictor of time to nontoxic methotrexate concentration ( P = 0.796; HR = 1.058; 95% CI = 0.692-1.617), and logistic regression demonstrated that levetiracetam was not a significant predictor of delayed elimination at any time point. Levetiracetam use was similar between groups when comparing patients experiencing delayed elimination at any time point with those without delayed elimination (13% vs 10%, respectively, P = 0.527). Conclusion: This study does not support the previous reports of a significant interaction between levetiracetam and methotrexate. A clinically significant interaction is unlikely in those without additional risk factors for delayed elimination.

scholarly journals Relationship of Dental Caries with Fluoride Concentration in Unstimulated whole Saliva of 7 to 15-year-old Nonfluoride Users

2014 ◽  
Vol 3 (2) ◽  
pp. 59-62
Author(s):  
Suresh Sharma ◽  
Manjit Talwar ◽  
Amrit Tewari ◽  
Harpinder Singh Chawla ◽  
Vinod Sachdev
Keyword(s):  
Dental Caries ◽  
Fluoride Concentration ◽  
Rank Correlation ◽  
Spearman’S Rank Correlation ◽  
Spearman’S Rank Correlation Coefficient ◽  
Whole Saliva ◽  
Fluoride Electrode ◽  
Unstimulated Whole Saliva ◽  
Relationship Of ◽  
The Relationship

ABSTRACT Objective The objective of the present study was to investigate the relationship between dental caries and fluoride concentration in unstimulated whole saliva of non-fluoride users. Study design Ninety subjects, aged 7 to 15 years who were non-fluoride users, participated in the study. Dental caries was recorded using modified Moller's index. Unstimulated whole saliva samples were collected early in the morning. Fluoride concentration in saliva was estimated using combination ion specific fluoride electrode (Orion). Water fluoride of the area ranged from 0.34 to 0.38 ppm. Results Salivary fluoride concentration (mean ± SE) in saliva (n = 90) was 0.03 ± 0.03 ppm with a range of 0.01 to 0.24 ppm. The DMFT ± SE was 3.26 ± 0.27 and DMFS 4.30 ± 0.42. Correlation of fluoride concentration in saliva was inverse and significant with DMFT (p < 0.01) and DMFS (p < 0.01) using the Spearman's rank correlation coefficient. Conclusion Fluoride concentration in saliva could be an indication of the individual's caries risk and help isolating high risk individuals requiring special chairside preventive strategies. How to cite this article Talwar M, Tewari A, Chawla HS, Sachdev V, Sharma S. Relationship of Dental Caries with Fluoride Concentration in Unstimulated whole Saliva of 7 to 15-year-old Nonfluoride Users. Int J Experiment Dent Sci 2014;3(2): 59-62.

Release of cariostatic agents from a new buffering fluoride- and xylitol-containing lozenge to human whole saliva in vivo

1997 ◽  
Vol 24 (5) ◽  
pp. 325-331 ◽  
Author(s):  
J. TENOVUO ◽  
T. HURME ◽  
A. AHOLA ◽  
C. SVEDBERG ◽  
I. OSTELA ◽  
...  
Keyword(s):  
Whole Saliva

scholarly journals Investigation of Changes in Saliva in Radiotherapy-Induced Head Neck Cancer Patients

2021 ◽  
Vol 18 (4) ◽  
pp. 1629
Author(s):  
Christina Winter ◽  
Roman Keimel ◽  
Markus Gugatschka ◽  
Dagmar Kolb ◽  
Gerd Leitinger ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Salivary Glands ◽  
Treatment Options ◽  
Chemical Properties ◽  
Head Neck Cancer ◽  
Potential Candidate ◽  
Adhesive Properties ◽  
Whole Saliva ◽  
Radiation Induced ◽  
Unstimulated Whole Saliva

The intact function of the salivary glands is of utmost importance for oral health. During radiotherapy in patients with head and neck tumors, the salivary glands can be damaged, causing the composition of saliva to change. This leads to xerostomia, which is a primary contributor to oral mucositis. Medications used for protective or palliative treatment often show poor efficacy as radiation-induced changes in the physico-chemical properties of saliva are not well understood. To improve treatment options, this study aimed to carefully examine unstimulated whole saliva of patients receiving radiation therapy and compare it with healthy unstimulated whole saliva. To this end, the pH, osmolality, electrical conductivity, buffer capacity, the whole protein and mucin concentrations, and the viscoelastic and adhesive properties were investigated. Moreover, hyaluronic acid was examined as a potential candidate for a saliva replacement fluid. The results showed that the pH of radiation-induced saliva shifted from neutral to acidic, the osmolality increased and the viscoelastic properties changed due to a disruption of the mucin network and a change in water secretion from the salivary glands. By adopting an aqueous 0.25% hyaluronic acid formulation regarding the lost properties, similar adhesion characteristics as in healthy, unstimulated saliva could be achieved.

scholarly journals Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study

2021 ◽  
Vol 18 (15) ◽  
pp. 8010
Author(s):  
Kohei Fujimori ◽  
Toshiki Yoneda ◽  
Takaaki Tomofuji ◽  
Daisuke Ekuni ◽  
Tetsuji Azuma ◽  
...  
Keyword(s):  
Cohort Study ◽  
Chronic Periodontitis ◽  
Characteristic Curve ◽  
Control Group ◽  
Discrimination Power ◽  
Whole Saliva ◽  
Supportive Periodontal Therapy ◽  
New Biomarkers ◽  
Unstimulated Whole Saliva ◽  
Attachment Level

The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group (n = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group (n = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group (p < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.

scholarly journals Essential role of glucose transporter GLUT3 for post-implantation embryonic development

2008 ◽  
Vol 200 (1) ◽  
pp. 23-33 ◽  
Author(s):  
S Schmidt ◽  
A Hommel ◽  
V Gawlik ◽  
R Augustin ◽  
N Junicke ◽  
...  
Keyword(s):  
Essential Role ◽  
Glucose Transporter ◽  
Growth Arrest ◽  
Complete Loss ◽  
Transporter Gene ◽  
Wild Type ◽  
Post Implantation ◽  
Time Point

Deletion of glucose transporter geneSlc2a3(GLUT3) has previously been reported to result in embryonic lethality. Here, we define the exact time point of growth arrest and subsequent death of the embryo.Slc2a3−/−morulae and blastocysts developed normally, implantedin vivo, and formed egg-cylinder-stage embryos that appeared normal until day 6.0. At day 6.5, apoptosis was detected in the ectodermal cells ofSlc2a3−/−embryos resulting in severe disorganization and growth retardation at day 7.5 and complete loss of embryos at day 12.5. GLUT3 was detected in placental cone, in the visceral ectoderm and in the mesoderm of 7.5-day-old wild-type embryos. Our data indicate that GLUT3 is essential for the development of early post-implanted embryos.

The Mobile Thriving Respite With and for the Street Community

2021 ◽  
pp. 002216782110467
Author(s):  
Robert McInerney ◽  
Kelsey Long ◽  
Rachel Stough
Keyword(s):  
Lived Experiences ◽  
Institutional Review Board ◽  
Short Term ◽  
Community Based ◽  
Bearing Witness ◽  
Institutional Review Board Approval ◽  
Institutional Review ◽  
Emergent Strategy ◽  
The Creation ◽  
Theoretical Foundations

We report on our work with the street community of Pittsburgh, specifically, a community-based action initiative we call the Mobile Thriving Respite (Institutional Review Board approval was obtained from our university). For 5 years, student advocate ethnographers from Point Park University have gathered data (e.g., long- and short-term interviews, participant-observations generating fieldnotes). The data revealed and supported the need for thriving beyond surviving homelessness. The data endorsed the creation of the mobile thriving respite. In the first part of this work, we will discuss some critical concepts regarding homelessness as a phenomenon and then argue that while surviving as enduring is necessary, there are some for whom survival is a perpetual, lethal state of being. We will discuss the theoretical foundations to the respite and offer researchers’ ethnographic accounts of the respite’s process and progress (We had to temporarily end the respite during the Covid-19 pandemic. To date, the respite has returned with “pop up” events outside at various locations). We will outline how the mobile thriving respite is a praxis as site of resistance as well as an emergent strategy, and an instantiation of communitas. We will then revisit surviving as collectively bearing witness and testifying to the lived experiences of those living outside.

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