Sodium Trimetaphosphate as a Novel Strategy for Matrix Metalloproteinase Inhibition and Dentin Remineralization

2018 ◽  
Vol 52 (3) ◽  
pp. 189-198 ◽  
Author(s):  
Rafael Simões Gonçalves ◽  
Polliana Mendes Candia Scaffa ◽  
Marina Ciccone Giacomini ◽  
Cristina de Mattos Pimenta Vidal ◽  
Heitor Marques Honório ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Polarized Light ◽  
Cross Sectional ◽  
Sodium Trimetaphosphate ◽  
Ph Cycling ◽  
Zymographic Analysis ◽  
Inhibitory Potential ◽  
Novel Strategy ◽  
Inhibited Enzyme

The effect of sodium trimetaphosphate (STMP) as an antiproteolytic and remineralizing agent on demineralized dentin was evaluated in vitro. The inhibitory potential of STMP at 0.5, 1.5, 3.5, and 5% against recombinant matrix metalloproteinases (MMPs) MMPs-2 and -9 was assessed by zymography. To investigate its remineralization potential, 40 bovine root specimens were obtained and subjected to a demineralization protocol to produce caries-like dentin lesions. After that, dentin surfaces were divided into 3 areas: (1) mineralized (no treatment); (2) demineralized; and (3) demineralized/treated with STMP and submitted to a pH-cycling associated or not with STMP (1.5, 3.5, or 5% STMP, 10 min of treatment). After that, superficial hardness (SH) and cross-sectional hardness (CSH) were determined. Polarized light microscopy (PLM) was used to qualitatively evaluate mineralization within the caries-like lesions. The zymographic analysis showed that STMP solution is a potent inhibitor of the gelatinolytic activity of MMPs-2 and -9 depending on the dose, since the lowest concentration (0.5%) partially inhibited the enzyme activity, while the higher concentrations completely inhibited enzyme activity. Regarding remineralization effect, only 1.5% STMP solution enhanced both the SH and CSH. PLM showed that the area treated with 1.5% STMP presented similar birefringence as mineralized sound dentin. In conclusion, 1.5% STMP solution is effective as an antiproteolytic agent against MMPs and promotes dentin remineralization.

scholarly journals Histological and microhardness evaluation of early artificial carious lesions in human and bovine enamel: in vitro study

2013 ◽  
Vol 16 (4) ◽  
pp. 49
Author(s):  
Allan Kenji Masuda ◽  
Mayra Fidelis Zamboni Quitero ◽  
Luciana Cardoso Espejo-Trung ◽  
Maria Aparecida Alves Cerqueira Luz
Keyword(s):  
Polarized Light ◽  
In Vitro Study ◽  
The Body ◽  
Polarization Microscopy ◽  
Cross Sectional ◽  
Enamel Caries ◽  
Carious Lesions ◽  
Ph Cycling ◽  
Bovine Enamel

<p><strong>Objective: </strong>Early carious lesions in bovine and humanenamel developed in vitro using a pH cycling regimenwere compared. <strong>Material and Methods: </strong>Fifteencentral bovine incisors and fifteen recently extractedhuman third molars were randomly divided into twogroups: ten for the cross-sectional microhardness test(MT) and five for polarized light microscopy (PLM)analysis. Enamel blocks measuring 5 x 5 mm weremade from the buccal face of the teeth. The blocksused for the MT were sliced into two halves: “A” and“B”. “A” slices were embedded in acrylic resin, withthe face of the dentin-enamel junction left exposedfor the MT prior to pH cycling. “B” slices and wholeblocks were coated with acid-resistant varnish,except a 3 x 3 mm central window, and submitted tothe pH cycling regimen (demineralizing solution for3 h and remineralizing solution for 21 h) over fiveconsecutive days. The “B” slices were then submittedto the MT and the whole blocks were processed forthe PLM study. <strong>Results: </strong>The PLM analysis revealedshallow, extensive lesions in the bovine enamel,hardly showing the superficial, dark and translucentzones, as well as deep cavity lesions in the humanenamel, with the body of the lesion and the darkzone evident. The MT revealed a significant decreasein microhardness in the superficial levels of thebovine enamel caries and at all depth levels of thehuman enamel caries. <strong>Conclusion: </strong>The pH cyclingregimen adopted led to the development of deeperand more demineralized carious lesions in humanenamel than bovine enamel</p><p>Keywords<br />Dental caries; Dental enamel; Microhardness tests; Polarization microscopy.</p>

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

scholarly journals Function of the N-terminal propeptide of an aminopeptidase from Vibrio proteolyticus

2000 ◽  
Vol 350 (3) ◽  
pp. 671-676 ◽  
Author(s):  
Zhen-Zhong ZHANG ◽  
Satoru NIRASAWA ◽  
Yoshiaki NAKAJIMA ◽  
Michiteru YOSHIDA ◽  
Kiyoshi HAYASHI
Keyword(s):  
Escherichia Coli ◽  
Enzyme Activity ◽  
Active Region ◽  
Protein Expression ◽  
Inclusion Bodies ◽  
Active Enzyme ◽  
Vibrio Proteolyticus ◽  
Show Evidence ◽  
Inhibited Enzyme

An aminopeptidase from Vibrio proteolyticus was translated as a preproprotein consisting of four domains: a signal peptide, an N-terminal propeptide, a mature region and a C-terminal propeptide. Protein expression and analysis of the activity results demonstrated that the N-terminal propeptide was essential to the formation of the active enzyme in Escherichia coli. Urea dissolution of inclusion bodies and dialysis indicated that the N-terminal propeptide could facilitate the correct folding of the enzyme in vitro. Using l-Leu-p-nitroanilide as the substrate, the kinetic parameters (kcat and Km) of the pro-aminopeptidase and processed aminopeptidases were analysed. The results suggested that the N-terminal propeptide inhibited enzyme activity of the mature region. In contrast, the C-terminal propeptide did not show evidence of forming an active enzyme, of correctly folding in vitro or of inhibiting the active region.

Effects of Fluoride Dentifrices on Enamel Lesion Formation

2008 ◽  
Vol 87 (3) ◽  
pp. 224-227 ◽  
Author(s):  
S. Toda ◽  
J.D. Featherstone
Keyword(s):  
Aqueous Solution ◽  
Aqueous Solutions ◽  
Ion Concentration ◽  
Fluoride Ion ◽  
Lesion Formation ◽  
Cross Sectional ◽  
Human Enamel ◽  
Amine Fluoride ◽  
Ph Cycling

An in vitro pH cycling model was used to test the hypothesis that the effects of 3 different fluoride compounds on de/remineralization are a function of the free fluoride ion concentration. Groups of 10 human enamel specimens were treated with one of: (a) amine fluoride (AmF), 1250 ppm F; (b) sodium monofluorophosphate (NaMFP), 1000 ppm F; (c) sodium fluoride (NaF), 1100 ppm F; (d) NaF, 250 ppm F; (e) Placebo (< 1 ppm F) dentifrices; or with aqueous solutions (f) NaF 900 ppm F or (g) NaF 30 ppm F. Lesions were assessed by cross-sectional microhardness. Mean ± SEM ΔZ (vol.% x μm) values of 3 dentifrices were: (a) 344 ± 155, (b) 4259 ± 257, and (c) 591 ± 83. The AmF (1250 ppm F) was not statistically significantly different from the NaF (1100 ppm F) dentifrice in this model. The NaMFP (1000 ppm F) dentifrice, without hydrolysis, had only the same efficacy as the NaF (30 ppm F) aqueous solution.

The effect of five fasciolicides on malate dehydrogenase activity and mortality of Fasciola gigantica, Fasciolopsis buski and Paramphistomum explanatum

1981 ◽  
Vol 55 (2) ◽  
pp. 115-122 ◽  
Author(s):  
A. J. Probert ◽  
R. K. Sharma ◽  
K. Singh ◽  
R. Saxena
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Malate Dehydrogenase ◽  
Fasciola Gigantica ◽  
High Concentrations ◽  
Fasciolopsis Buski ◽  
Reduced Activity ◽  
Inhibited Enzyme ◽  
Malate Oxidation

ABSTRACTThe effect of oxyclozanide, hexachlorophene, nitroxynil, rafoxanide and diamphenethide on malate dehydrogenase activity of homogenates of Fasciola gigantica, Fasciolopsis buski and Paramphistomum explanatum was investigated. The ratio of oxaloacetate reduction to malate oxidation in homogenates of Fasciola gigantica, Fasciolopsis buski and P. explanatum was 4·5:1, 3·6:1 and 5·2:1 respectively. Oxyclozanide and rafoxanide at 10−3 M inhibited enzyme activity by 100% in homogenates from all three species while hexachlorophene at 10−3M also caused 100% inhibition in homogenates from Fasciola gigantica and P. explanatum but only 65% of malate oxidation in Fasciolopsis buski homogenates. Nitroxynil at 10−3M produced 60% inhibition in F. buski homogenates yet had little effect at this concentration on preparations from the other species. Little inhibition was seen with diamphenethide, even at high concentrations. Rapid death of Fasicola gigantica and P. explanatum resulted in vitro when 10−3M oxyclozanide, hexachlorophene, nitroxynil or rafoxanide, were added to the incubation medium. Fasciolopsis buski was killed by 10−3M oxyclozanide but at this concentration the remaining compounds only caused reduced activity. Assay of malate dehydrogenase following drug treatment in vitro failed to show any appreciable reduction in enzyme activity in Fasciola gigantica and P. explanatum but oxyclozanide and hexachlorophene produced inhibition in Fasciolopsis buski. The mode of action of these compounds is discussed.

scholarly journals pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization

2008 ◽  
Vol 19 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Celso Silva Queiroz ◽  
Anderson Takeo Hara ◽  
Adriana Franco Paes Leme ◽  
Jaime Aparecido Cury
Keyword(s):  
Dose Response ◽  
Polarized Light ◽  
Positive Control ◽  
Cross Sectional ◽  
Response Effect ◽  
Ph Cycling ◽  
Fluoride Dentifrice ◽  
Dose Response Effect ◽  
Bovine Enamel ◽  
Mineral Loss

Since the currently available pH-cycling models do not differentiate the anti-caries potential of dentifrices with low fluoride (F) concentration, two models were developed and tested in the present. Bovine enamel blocks were subjected to the models and treated with F solutions containing from 70 to 280 mug F/mL in order to validate them in terms of dose-response effect. The models were also tested by evaluating the dentifrices Colgate Baby (500 mug F/g, as a low fluoride dentifrice), Tandy (1,100 mug F/g, as an active F-dentifrice) and Crest (1,100 mug F/g, as positive control). Enamel mineral loss or gain was assessed by surface and cross-sectional microhardness, and lesion depth was analyzed by polarized light microscopy. The pH-cycling models showed F dose-response effect either reducing enamel demineralization or enhancing remineralization. The low F dentifrice presented anti-caries potential, but it was not equivalent to the dentifrices containing 1,100 mug F/g. These data suggest that the models developed in this study were able to evaluate the anti-caries potential of low F dentifrice either on resistance to demineralization or on enhancement of remineralization.

Effect of Heavy Metals Contamination from Cigarette Smoke on Sound and Caries-Like Enamel

2018 ◽  
Vol 24 (6) ◽  
pp. 762-767 ◽  
Author(s):  
Jéssica D. Theobaldo ◽  
Waldemir F. Vieira-Junior ◽  
Anderson Catelan ◽  
Maria do Carmo A. Mainardi ◽  
Orlando A. Ysnaga ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Cigarette Smoke ◽  
Surface Microhardness ◽  
Treatment Control ◽  
Cross Sectional ◽  
X Ray ◽  
Heavy Metals Contamination ◽  
Heavy Metal Deposition ◽  
Ph Cycling

AbstractIn this study, we sought to evaluate the influence of cigarette smoke and pH cycling on the chemical composition and surface/cross-sectional enamel microhardness. A total of 40 dental blocks obtained from bovine incisors were divided into four groups (n=10): no treatment (control); exposure to cigarette smoke (CS); exposure to pH cycling (PC); and exposure to cigarette smoke and pH cycling (CS-PC). The samples were analyzed by synchrotron radiation micro X-ray fluorescence, bench mode X-ray fluorescence, as well as surface microhardness (SMH) and cross-sectional microhardness (CSMH) testing. The SMH results were submitted to analysis of variance (ANOVA) and Tukey’s test. The CSMH results were evaluated using split-plot ANOVA and Tukey’s test. A high amount of Cd and Pb and traces of Ni and As were observed in enamel and dentin after exposure to cigarette smoke (CS and CS-PC). The SMH and CSMH of CS were statistically higher when compared with the control. The PC and CS-PC showed lower SMH and CSMH. We conclude that exposure to cigarette smoke promoted heavy metal deposition in enamel/dentin. In addition, it increased the enamel microhardness but did not promote a protective effect on the in vitro development of caries. The clinical significance of this work is that there is significant bioaccumulation of heavy metals from cigarette smoke on the surface and in the enamel and dentin.

scholarly journals Effect of Trimetaphosphate and Fluoride Association on Hydroxyapatite Dissolution and Precipitation In Vitro

2014 ◽  
Vol 25 (6) ◽  
pp. 479-484 ◽  
Author(s):  
Alberto Carlos Botazzo Delbem ◽  
José Antonio Santos Souza ◽  
Ana Carolina Soares Fraga Zaze ◽  
Eliana Mitsue Takeshita ◽  
Kikue Takebayashi Sassaki ◽  
...  
Keyword(s):  
Calcium Fluoride ◽  
Calcium Concentration ◽  
Ph Value ◽  
X Ray Diffraction ◽  
X Ray ◽  
Sodium Trimetaphosphate ◽  
Hydroxyapatite Powder ◽  
Calcium Phosphorus ◽  
Ph Cycling

The present study analyzed the action of sodium trimetaphosphate (TMP) and/or fluoride on hydroxyapatite. Hydroxyapatite powder was suspended in different solutions: deionized water, 500 µg F/mL, 1,100 µg F/mL, 1%TMP, 3%TMP, 500 µg F/mL plus 1%TMP and 500 µg F/mL plus 3%TMP. The pH value of the solutions was reduced to 4.0 and after 30 min, raised to 7.0 (three times). After pH-cycling, the samples were analyzed by X-ray diffraction and infrared spectroscopy. The concentrations of calcium fluoride, fluoride, calcium and phosphorus were also determined. Adding 1% or 3% TMP to the solution containing 500 µg F/mL produced a higher quantity of calcium fluoride compared to samples prepared in a 1,100 µg F/mL solution. Regarding the calcium concentration, samples prepared in solutions of 1,100 µg F/mL and 500 µg F/mL plus TMP were statistically similar and showed higher values. Using solutions of 1,100 µg F/mL and 500 µg F/mL plus TMP resulted in a calcium/phosphorus ratio close to that of hydroxyapatite. It is concluded that the association of TMP and fluoride favored the precipitation of a more stable hydroxyapatite.

scholarly journals In VitroProperties of Orthodontic Adhesives with Fluoride or Amorphous Calcium Phosphate

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Clara Ka Wai Chow ◽  
Christine D. Wu ◽  
Carla A. Evans
Keyword(s):  
Calcium Phosphate ◽  
Bacterial Adhesion ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Lesion Formation ◽  
X Ray ◽  
Orthodontic Adhesives ◽  
Ph Cycling ◽  
Phosphorous Content

Thisin vitrostudy evaluated the efficacy of orthodontic adhesives with fluoride or amorphous calcium phosphate (ACP) in reducing bacterial adhesion and enamel demineralization. Forty human premolars each sectioned buccolingually into three parts were bracketed with control resin (Transbond XT) or adhesives containing ACP (Aegis Ortho) or fluoride (QuickCure). Artificial lesions induced by pH cycling were examined by X-ray photoelectron spectrophotometry (XPS) and polarized light microscopy (PLM). After 28 days, Aegis Ortho demonstrated the lowest calcium and phosphorous content by XPS analysis. After 42 days, reductions in lesion depth areas were 23.6% for Quick Cure and 20.3% for Aegis Ortho (P<0.05). In the presence of 1% sucrose, adhesion ofStreptococcus mutansto Aegis Ortho and Quick Cure was reduced by 41.8% and 37.7% (P<0.05) as compared to Transbond XT. Composites containing ACP or fluoride reduced bacterial adherence and lesion formation as compared to a composite without ACP or fluoride.

scholarly journals Enamel remineralization by fluoride-releasing materials: proposal of a pH-cycling model

2010 ◽  
Vol 21 (5) ◽  
pp. 446-451 ◽  
Author(s):  
Eliana Rodrigues ◽  
Alberto Carlos Botazzo Delbem ◽  
Denise Pedrini ◽  
Luciana Cavassan
Keyword(s):  
Dose Response ◽  
Response Relationship ◽  
Surface Microhardness ◽  
Dose Response Relationship ◽  
Cross Sectional ◽  
Microhardness Test ◽  
Significant Difference ◽  
Ph Cycling ◽  
And Control

This study proposes a pH-cycling model for verifying the dose-response relationship in fluoride-releasing materials on remineralization in vitro. Sixty bovine enamel blocks were selected for the surface microhardness test (SMH1). Artificial caries lesions were induced and surface microhardness test (SMH2) was performed. Forty-eight specimens were prepared with Z 100, Fluroshield, Vitremer and Vitremer ¼ diluted - powder/liquid, and subjected to a pH-cycling model to promote remineralization. After pH-cycling, final surface microhardness (SMH3) was assessed to calculate percent recovery of surface microhardness (%SMHR). Fluoride present in enamel (μg F/mm3) and in the pH-cycling solutions (μg F) was measured. Cross-sectional microhardness was used to calculate mineral content (∆Z). There was no significant difference between Z 100 and control groups on analysis performed on - %SMHR, ∆Z, μg F and mg F/mm3 (p>0.05). Results showed a positive correlation between %SMHR and μg F/mm3 (r=0.9770; p=0.004), %SMHR and μg F (r=0.9939; p=0.0000001), ∆ and μg F/mm3 (r=0.9853; p=0.0002), ∆ and μg F (r=0.9975; p=0.0000001) and between μg F/mm3 and μg F (r=0.9819; p=0.001). The pH-cycling model proposed was able to verify in vitro dose-response relationship of fluoride-releasing materials on remineralization.

Sign in / Sign up

Export Citation Format

Share Document