scholarly journals In VitroProperties of Orthodontic Adhesives with Fluoride or Amorphous Calcium Phosphate

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Clara Ka Wai Chow ◽  
Christine D. Wu ◽  
Carla A. Evans
Keyword(s):  
Calcium Phosphate ◽  
Bacterial Adhesion ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Lesion Formation ◽  
X Ray ◽  
Orthodontic Adhesives ◽  
Ph Cycling ◽  
Phosphorous Content

Thisin vitrostudy evaluated the efficacy of orthodontic adhesives with fluoride or amorphous calcium phosphate (ACP) in reducing bacterial adhesion and enamel demineralization. Forty human premolars each sectioned buccolingually into three parts were bracketed with control resin (Transbond XT) or adhesives containing ACP (Aegis Ortho) or fluoride (QuickCure). Artificial lesions induced by pH cycling were examined by X-ray photoelectron spectrophotometry (XPS) and polarized light microscopy (PLM). After 28 days, Aegis Ortho demonstrated the lowest calcium and phosphorous content by XPS analysis. After 42 days, reductions in lesion depth areas were 23.6% for Quick Cure and 20.3% for Aegis Ortho (P<0.05). In the presence of 1% sucrose, adhesion ofStreptococcus mutansto Aegis Ortho and Quick Cure was reduced by 41.8% and 37.7% (P<0.05) as compared to Transbond XT. Composites containing ACP or fluoride reduced bacterial adherence and lesion formation as compared to a composite without ACP or fluoride.

scholarly journals Comparison of the Efficacy of Different Fluoride Varnishes on Dentin Remineralization During a Critical pH Exposure Using Quantitative X-Ray Microtomography

2018 ◽  
Vol 43 (6) ◽  
pp. E308-E316
Author(s):  
A Sleibi ◽  
A Tappuni ◽  
D Mills ◽  
GR Davis ◽  
A Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Mineral Content ◽  
Test Group ◽  
Control Group ◽  
Mineral Concentration ◽  
Voxel Size ◽  
X Ray ◽  
Casein Phosphopeptide

SUMMARY Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide–amorphous calcium phosphate, fluoride and bioglass, or fluoride alone. Methods and Materials: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide–amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis. Results: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride (p≤0.05 and p≤0.001), 25% BGA (p≤0.001), and 104% fluoride alone varnish (p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% (p≤0.05 and p≤0.001). Conclusions: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.

scholarly journals Kemampuan Bioaktif Glass (Novamin) dan Casein Peptide Amorphous Calcium Phosphate (CPP-ACP) terhadap Demineralisasi Enamel

2019 ◽  
Vol 7 (2) ◽  
pp. 111
Author(s):  
Jeanny Kathleen H ◽  
Cecilia G.J. Lunardhi ◽  
Ari Subiyanto
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Pathological State ◽  
X Ray ◽  
Group A ◽  
Ph Cycling ◽  
Casein Phosphopeptide ◽  
Group D ◽  
Scanning Electron ◽  
Better Than

Background: Oral cavity is always associated with a dynamic atmosphere where the process of demineralization and remineralization will continue to occur. Caries is a pathological state of continuous demineralization process. Prevention of the occurrence of demineralization process can be done by enhancing remineralization, materials that can be used materials that can trigger a process of remineralization include fluoride, cpp-acp, and novamin. Purpose: The aim of this study was to evaluate and compare the potential of bioactive-Glass (Novamin) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing dentifrice on enamel demineralization. Method: A total of 24 sound human premolars were divided into 4 groups and continued into pH cycling regime to be evaluated with Scanning Electron Microscope – Energy Dispersive X-Ray. Result: Group D showed significantly higher values (P < 0.05) when compared with less demineralization lesion than Group A, B, and C. Conclusions: The ability of Novamin on demineralization lesions better than CPP-ACP.

scholarly journals Effectiveness of Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) Compared to Fluoride Products in an In-Vitro Demineralization Model

Materials ◽  
2021 ◽  
Vol 14 (20) ◽  
pp. 5974
Author(s):  
Markus Reise ◽  
Stefan Kranz ◽  
Markus Heyder ◽  
Klaus D. Jandt ◽  
Bernd W. Sigusch
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Positive Control ◽  
Negative Control ◽  
Control Groups ◽  
Casein Phosphopeptide ◽  
Tooth Mousse ◽  
Negative Controls

The goal of this study was to evaluate the effectiveness of the toothpaste Tooth Mousse compared to conventional fluoride-based versions in the prevention of enamel and dentin demineralization. Human enamel and dentin samples (n = 120 each) were exposed to artificial demineralization at pH 4.92. During the demineralization process, the samples in the test groups were periodically treated with Tooth Mousse (TM) containing casein-phosphopeptide -amorphous-calcium-phosphate (CPP-ACP) and Tooth Mousse Plus (TMP) containing amorphous-calcium-fluoride-phosphate (CPP-ACPF) to evaluate their protective properties. Fluoride toothpastes containing 1400 ppm amine fluoride (AmF) and 1450 ppm sodium fluoride (NaF) were applied in the positive control groups. Treatment with distilled water (group C-W) or demineralization without treatment (group C-D) served as negative controls. After the demineralization and treatment process, all samples were cut longitudinally and lesion depths were determined at six locations using polarized light microscopy. In TM/TMP groups (enamel: 80/86 µm, dentin: 153/156 µm) lesion depths were significantly smaller compared to the negative control groups C-W/C-D (enamel: 99/111 µm, dentin: 163/166 µm). However, TM and TMP compared to the positive controls AmF/NaF (enamel: 58/63 µm, dentin: 87/109 µm) showed higher lesion depths. The application of TM/TMP (89%/78%) during demineralization led to a reduced number of severe lesions compared to the negative controls C-W/C-D (100%/95%). In this study we demonstrate that Tooth Mousse is less effective regarding prevention of enamel and dentin demineralization compared to fluoride containing toothpastes.

scholarly journals The longevity of casein phosphopeptide–amorphous calcium phosphate fluoride varnish's preventative effects: Assessment of white spot lesion formation

2018 ◽  
Vol 89 (1) ◽  
pp. 10-15
Author(s):  
Moufida Abufarwa ◽  
Amal Noureldin ◽  
Phillip M Campbell ◽  
Peter H Buschang
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Polarized Light ◽  
Fluoride Varnish ◽  
Control Group ◽  
White Spot Lesion ◽  
Enamel Demineralization ◽  
Casein Phosphopeptide ◽  
Experimental Group

ABSTRACT Objectives: To test how long casein phosphopeptide–amorphous calcium phosphate (CPP-ACP) fluoride varnish prevents enamel demineralization in vitro. Materials and Methods: Human molars and premolars were sectioned buccolingually and randomly assigned to two groups. Standardized pretreatment images of enamel surfaces were obtained using FluoreCam. The control group received no treatment, and the experimental group received an application of CPP-ACP fluoride varnish. Over simulated periods of 2, 4, 8, and 12 weeks, specimens were placed in a toothbrushing simulator, thermocycled, subjected to 9 days of pH cycling, and imaged with FluoreCam. Samples were sectioned and polished for polarized light microscope (PLM) evaluation. Results: There were statistically significant time (P &lt; .001) and varnish (P &lt; .001) effects on area, intensity, and impact of enamel demineralization. The control group showed significant and progressive demineralization over the 12 weeks (P &lt; .001). The experimental group revealed no significant demineralization during the first 4 weeks (P &gt; .05) and significant (P &lt; .001) increases thereafter. Experimental demineralization after 12 weeks was comparable to 2-week demineralization in the controls, with significant between-group differences (P &lt; .001) in enamel demineralization at all time points. PLM of the control and experimental groups revealed lesion depths of 90 ± 34 μm and 37 ± 9 μm, respectively. Conclusions: Within the limitations of this in vitro study, CPP-ACP fluoride varnish prevents enamel demineralization for at least 4 weeks and limits demineralization up to 12 weeks.

Fabrication and evaluation of calcium phosphate cement scaffold with controlled internal channel architecture and complex shape

2007 ◽  
Vol 221 (8) ◽  
pp. 951-958 ◽  
Author(s):  
X Li ◽  
D Li ◽  
B Lu ◽  
L Wang ◽  
Z Wang
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Complex Shape ◽  
X Ray Diffraction ◽  
X Ray ◽  
Precise Control ◽  
Internal Channel ◽  
Channel Architecture

The ability to have precise control over internal channel architecture, porosity, and external shape is essential for tissue engineering. The feasibility of using indirect stereo-lithography (SL) to produce scaffolds from calcium phosphate cement materials for bone tissue engineering has been investigated. The internal channel architecture of the scaffolds was created by removal of the negative resin moulds made with SL. Scanning electron microscopy (SEM) showed highly open, well-interconnected channel architecture. The X-ray diffraction examination revealed that the hydroxyapatite phase formed at room temperature in the cement was basically stable up to 850 °C. There was no phase decomposition of hydroxyapatite, although the crystallinity and grain size were different. The ability of resulting structure to support osteoblastic cells culture was tested in vitro. Cells were evenly distributed on exterior surfaces and grew into the internal channels of scaffolds. To exploit the ability of this technique, anatomically shaped femoral supracondylar scaffolds with 300-800 μm interconnected channels were produced and characterized.

scholarly journals Lactoferrin/Calcium Phosphate-Modified Porous Ti by Biomimetic Mineralization: Effective Infection Prevention and Excellent Osteoinduction

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 992
Author(s):  
Song Chen ◽  
Yuanli He ◽  
Linna Zhong ◽  
Wenjia Xie ◽  
Yiyuan Xue ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Photoelectron Spectroscopy ◽  
Antibacterial Properties ◽  
Biomimetic Mineralization ◽  
Matrix Mineralization ◽  
X Ray ◽  
Porous Ti ◽  
Potential Applications ◽  
Modification Of Titanium

The surface modification of titanium (Ti) can enhance the osseointegration and antibacterial properties of implants. In this study, we modified porous Ti discs with calcium phosphate (CaP) and different concentrations of Lactoferrin (LF) by biomimetic mineralization and examined their antibacterial effects and osteogenic bioactivity. Firstly, scanning electron microscopy (SEM), the fluorescent tracing method, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and the releasing kinetics of LF were utilized to characterize the modified Ti surface. Then, the antibacterial properties against S. sanguis and S. aureus were investigated. Finally, in vitro cytological examination was performed, including evaluations of cell adhesion, cell differentiation, extracellular matrix mineralization, and cytotoxicity. The results showed that the porous Ti discs were successfully modified with CaP and LF, and that the LF-M group (200 μg/mL LF in simulated body fluid) could mildly release LF under control. Further, the LF-M group could effectively inhibit the adhesion and proliferation of S. sanguis and S. aureus and enhance the osteogenic differentiation in vitro with a good biocompatibility. Consequently, LF-M-modified Ti may have potential applications in the field of dental implants to promote osseointegration and prevent the occurrence of peri-implantitis.

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