Analysis of the Primary Sex Ratio, Sex Chromosome Aneuploidy and Diploidy in Human Sperm Using Dual-Colour Fluorescence in situ Hybridisation

1993 ◽  
Vol 1 (4) ◽  
pp. 325-334 ◽  
Author(s):  
A.S.H. (a) Goldman ◽  
Z. (a) Fomina ◽  
P.A. (a) Knights ◽  
C.J. (a) Hill ◽  
A.P. (b) Walker ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Sex Chromosome ◽  
In Situ Hybridisation ◽  
Human Sperm ◽  
Fluorescence In Situ Hybridisation ◽  
Sex Chromosome Aneuploidy ◽  
Primary Sex Ratio ◽  
Chromosome Aneuploidy ◽  
Dual Colour

scholarly journals Sperm sex chromosome fluorescence in situ hybridisation in pygmy hippopotamus

2012 ◽  
Author(s):  
Joseph Saragusty ◽  
Joseph Saragusty ◽  
Robert Hermes ◽  
Heribert Hofer ◽  
Tim Bouts ◽  
...  
Keyword(s):  
Sex Chromosome ◽  
In Situ Hybridisation ◽  
Fluorescence In Situ Hybridisation

scholarly journals Detection of t(7;12)(q36;p13) in paediatric leukaemia using dual colour fluorescence in situ hybridisation

2015 ◽  
Vol 3 (1) ◽  
pp. 4 ◽  
Author(s):  
Temitayo Owoka ◽  
Michael Vetter ◽  
Concetta Federico ◽  
Salvatore Saccone ◽  
Sabrina Tosi
Keyword(s):  
In Situ Hybridisation ◽  
Fluorescence In Situ Hybridisation ◽  
Dual Colour ◽  
Paediatric Leukaemia

Factors affecting the timing and imprinting of replication on a mammalian chromosome

1995 ◽  
Vol 108 (8) ◽  
pp. 2801-2809
Author(s):  
W.A. Bickmore ◽  
A.D. Carothers
Keyword(s):  
In Situ Hybridisation ◽  
Replication Timing ◽  
Histone Deacetylation ◽  
Fluorescence In Situ Hybridisation ◽  
Phase Detection ◽  
Chromosome 11 ◽  
Single Chromosome ◽  
Factors Affecting ◽  
Dual Colour

Fluorescence in situ hybridisation has been used to follow replication of the short arm of human chromosome 11 using chromosome anomalies to distinguish the maternally-and paternally-derived homologues. The temporal difference in replication timing within and between chromosomes has been estimated by combining S phase detection with dual colour fluorescence in situ hybridisation. Proximal regions of 11p, including the WT1 gene, tend to replicate earlier on the maternally-derived chromosome than on the paternally-derived homologue. More distal parts of 11p (including the IGF2 gene) have the opposite imprint. The average difference in replication timing between homologous loci in the population of cells is small compared to the differences between loci along a single chromosome. The imprint is not strictly adhered to since many nuclei have hybridisation patterns opposite to the trend within the population. The nature of the imprinting signal has been investigated. Absolute replication time, but not the imprint, was affected by azacytidine, an inhibitor of DNA methylation. The replication imprint was modified by treatments that inhibit histone deacetylation. We suggest that replication imprinting reflects differences in chromatin structure between homologues.

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