scholarly journals Limbal Stem Cell-Sparing Corneoscleroplasty with Peripheral Intralamellar Tuck: A New Surgical Technique for Keratoglobus

2017 ◽  
Vol 8 (1) ◽  
pp. 279-287
Author(s):  
Elias Jarade ◽  
Rafic Antonios ◽  
Sylvain el-Khoury
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Structural Integrity ◽  
Single Step ◽  
Limbal Stem Cells ◽  
Host Cornea ◽  
Limbal Stem Cell ◽  
The Right ◽  
New Surgical Technique

Purpose: To describe the technique of limbal stem cell-sparing corneoscleroplasty for the management of advanced keratoglobus. Methods: A patient with bilateral advanced keratoglobus, with best-corrected visual acuity of 20/400 in the right eye and 20/200 in the left eye, underwent limbal stem cell-sparing corneoscleroplasty of the right eye. Initially, a 360-degree limbal incision with 200-μm depth was created, followed by a sublimbal tunnel dissection into the sclera, in order to conserve stem cells. Next, a limbus-to-limbus lamellar keratectomy at 200-μm depth was performed. Meanwhile, a donor corneoscleral button with preserved endothelium of the central 8 mm was fashioned. Prior to suturing the donor corneoscleral graft using a modified suturing technique to cover its scleral component, a full-thickness trephination of 8-mm diameter was completed in the central host cornea. Results: Reepithelialization occurred within the first week. No episodes of rejection, intraocular pressure spikes, or epithelial breakdown were observed postoperatively. At the 6-month follow-up, the patient had 20/70 best-corrected vision and a smooth cornea with regular astigmatism on topography. Conclusion: Limbal stem cell-sparing corneoscleroplasty is a single-step technique for restoring the structural integrity of the cornea in advanced keratoglobus while preserving the host limbal stem cells.

scholarly journals Sclerocorneal limbal stem cell autograft transplantation in dogs

2007 ◽  
Vol 59 (5) ◽  
pp. 1194-1204 ◽  
Author(s):  
A.T.J. Brunelli ◽  
F.A.M. Vicente ◽  
F. Chahud ◽  
A.P. Oriá ◽  
A.A. Bolzan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Goblet Cells ◽  
Control Group ◽  
Post Transplantation ◽  
Limbal Stem Cells ◽  
Corneal Transparency ◽  
Limbal Stem Cell ◽  
The Right ◽  
Limbal Transplantation

The effects of sclerocorneal limbal stem cell autograft transplantation in dogs with corneal wounds were studied. Eighteen dogs were divided in two groups (GI and GII). The animals of GI (n=12) underwent limbal transplantation 30 days after the destruction of limbal stem cells. The dogs of GII (n=6) only underwent destruction of stem cells (control group). Light microscopy examination of the right eye was performed on days 3, 7, 14, 30, 60, and 120 after limbal transplantation (GI), and on days 33, 37, 44, 60, 90, and 150 after limbal destruction (GII). Results showed a complete destruction of limbal stem cells with loss of corneal transparency. Limbal transplantation prevented conjunctivalization in grafted area. Corneal vascularization and a 360º corneal conjunctivalization were noted in the control dogs (GII). Corneal transparency was restored from day 60th after surgery. Histological examination did not distinguish the transition between the graft and the normal corneal epithelium at anytime. Goblet cells were found in control animals (GII) on 33, 37, 60, and 150 days, whereas a single grafted dog (GI) presented a few goblet cells on day 60th post-transplantation. Limbal autograft transplantation was effective in restoring corneal clarity with no development of ocular complications.

scholarly journals Limbal stem cell deficiency: etiology, pathogenesis, priniciples and prospects of surgical treatment

2019 ◽  
Vol 12 (1) ◽  
pp. 103-111
Author(s):  
A. S. Dubovikov ◽  
I. O. Gavrilyuk ◽  
A. N. Kulikov ◽  
S. V. Churashov ◽  
V. F. Chernysh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Surgical Treatment ◽  
Ex Vivo ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cells ◽  
History Of Development ◽  
History Of ◽  
Limbal Epithelial Stem Cells ◽  
Limbal Stem Cell

The review is focused on the modern view of the etiology and pathogenesis of limbal stem cells deficiency. The history of development of tissue and ex-vivo transplantation of limbal epithelial stem cells is presented. Certain promising directions of the treatment of patients with limbal stem cells deficiency are presented.

scholarly journals PALT (Partial Allogeneic Limbal Transplantation): A New Surgical Technique For Limbal Stem Cell Deficiency

2021 ◽  
Author(s):  
Christiane Kesper ◽  
Anja Viestenz ◽  
Thomas Hammer ◽  
Joana Heinzelmann ◽  
Sabine Foja ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Stem Cell ◽  
Surgical Technique ◽  
Limbal Stem Cell Deficiency ◽  
Corneal Vascularization ◽  
Donor Graft ◽  
Limbal Stem Cell ◽  
New Surgical Technique ◽  
Limbal Transplantation

Abstract PurposeLimbal stem cell deficiency (LSCD) is a rare but extremely relevant disease of the eye. LSCD patients often require a variety of surgical procedures, including keratoplasty in some cases. However, the outcome of these surgeries, including opacification and revascularization, is often frustrating due to LSCD relapse.MethodsWe developed a new surgical technique for the treatment of LSCD in which partial allogenic limbal transplantation (PALT) is carried out as part of penetrating keratoplasty (PK). After the PK, 1-8 slices from the limbal tissue of the donor graft are prepared and placed under the double running sutures attaching the corneal graft. This procedure was performed on 14 patients with LSCD, caused by severe ocular burn in 5 cases and by infection in 9. Between one and eight limbal transplants were used depending on the extension of the LSCD. ResultsAll 14 patients showed stable or increased visual acuity after the PALT surgery compared to their preoperative visual acuity. All of the grafts were integrated into the superficial corneal layers without progression of corneal vascularization beyond the limbal grafts. The median follow-up period was 12 months on average.ConclusionThe PALT method seems to be a promising surgical procedure for the treatment of patients with LSCD. It can be properly carried out in the context of keratoplasty and does not require a separate donor tissue. The PALT grafts may offer the possibility of constructing a new limbal region, resulting in stable or even increased visual acuity and the absence of corneal vascularization.

AUTOLOGOUS FIBRIN-CULTURED LIMBAL STEM CELLS PERMANENTLY RESTORE THE CORNEAL SURFACE OF PATIENTS WITH TOTAL LIMBAL STEM CELL DEFICIENCY1

2001 ◽  
Vol 72 (9) ◽  
pp. 1478-1485 ◽  
Author(s):  
Paolo Rama ◽  
Stefano Bonini ◽  
Alessandro Lambiase ◽  
Osvaldo Golisano ◽  
Patrizia Paterna ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Limbal Stem Cells ◽  
Corneal Surface ◽  
Limbal Stem Cell

scholarly journals Clinical Trials of Limbal Stem Cell Deficiency Treated with Oral Mucosal Epithelial Cells

2020 ◽  
Vol 21 (2) ◽  
pp. 411 ◽  
Author(s):  
Joan Oliva ◽  
Fawzia Bardag-Gorce ◽  
Yutaka Niihara
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Corneal Epithelium ◽  
Contact Lenses ◽  
Embryonic Stem ◽  
Epithelial Stem Cells ◽  
Physical Damage ◽  
Limbal Stem Cells ◽  
Cell Therapies ◽  
Limbal Stem Cell

The corneal surface is an essential organ necessary for vision, and its clarity must be maintained. The corneal epithelium is renewed by limbal stem cells, located in the limbus and in palisades of Vogt. Palisades of Vogt maintain the clearness of the corneal epithelium by blocking the growth of conjunctival epithelium and the invasion of blood vessels over the cornea. The limbal region can be damaged by chemical burns, physical damage (e.g., by contact lenses), congenital disease, chronic inflammation, or limbal surgeries. The degree of limbus damage is associated with the degree of limbal stem cells deficiency (partial or total). For a long time, the only treatment to restore vision was grafting part of the healthy cornea from the other eye of the patient or by transplanting a cornea from cadavers. The regenerative medicine and stem cell therapies have been applied to restore normal vision using different methodologies. The source of stem cells varies from embryonic stem cells, mesenchymal stem cells, to induced pluripotent stem cells. This review focuses on the use of oral mucosa epithelial stem cells and their use in engineering cell sheets to treat limbal stem cell deficient patients.

scholarly journals Limbal stem cell deficiency: special focus on tracking limbal stem cells

2016 ◽  
Vol 40 ◽  
pp. 1059-1067
Author(s):  
Santhosh KACHAM ◽  
Bhaskar BIRRU ◽  
Sreenivasa Rao PARCHA ◽  
Ramaraju BAADHE
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Limbal Stem Cell Deficiency ◽  
Special Focus ◽  
Limbal Stem Cells ◽  
Limbal Stem Cell
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