Effect of Storage Temperature on the Activity of Superoxide Dismutase, Catalase, Glutathione Peroxidase, Glutathione Reductase and Glutathione S-Transferase in Rat Liver and Kidney Homogenates

1993 ◽  
Vol 47 (3) ◽  
pp. 149-155 ◽  
Author(s):  
Klaus Jung ◽  
Sven Kühler ◽  
Silke Klotzek ◽  
Sabine Becker ◽  
Wolfgang Henke
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Rat Liver ◽  
Storage Temperature ◽  
Glutathione S Transferase ◽  
Liver And Kidney

scholarly journals Effect of Cross-Sex Hormonal Replacement on Antioxidant Enzymes in Rat Retroperitoneal Fat Adipocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Israel Pérez-Torres ◽  
Verónica Guarner-Lans ◽  
Alejandra Zúñiga-Muñoz ◽  
Rodrigo Velázquez Espejel ◽  
Alfredo Cabrera-Orefice ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Enzymatic Activities ◽  
Glutathione S Transferase ◽  
Control Groups ◽  
Intact Female ◽  
Hormonal Replacement

We report the effect of cross-sex hormonal replacement on antioxidant enzymes from rat retroperitoneal fat adipocytes. Eight rats of each gender were assigned to each of the following groups: control groups were intact female or male (F and M, resp.). Experimental groups were ovariectomized F (OvxF), castrated M (CasM), OvxF plus testosterone (OvxF + T), and CasM plus estradiol (CasM + E2) groups. After sacrifice, retroperitoneal fat was dissected and processed for histology. Adipocytes were isolated and the following enzymatic activities were determined: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). Also, glutathione (GSH) and lipid peroxidation (LPO) were measured. In OvxF, retroperitoneal fat increased and adipocytes were enlarged, while in CasM rats a decrease in retroperitoneal fat and small adipocytes are observed. The cross-sex hormonal replacement in F rats was associated with larger adipocytes and a further decreased activity of Cu-Zn SOD, CAT, GPx, GST, GR, and GSH, in addition to an increase in LPO. CasM + E2exhibited the opposite effects showing further activation antioxidant enzymes and decreases in LPO. In conclusion, E2deficiency favors an increase in retroperitoneal fat and large adipocytes. Cross-sex hormonal replacement in F rats aggravates the condition by inhibiting antioxidant enzymes.

scholarly journals Oxidative stress and antioxidative defense in the skin of rats with thermal injury

2003 ◽  
Vol 22 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Bato Korac ◽  
Biljana Buzadzic
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Control Level ◽  
Thioredoxin Reductase ◽  
Skin Area ◽  
Antioxidative Defense ◽  
Glutathione S Transferase

Changes in the activity and level of some antioxidative defense system components were determined in the rat skin during hypo- (ebb) and hypermetabolic (flow) phase of thermal trauma. At the same time, the effects of enzymatic (superoxide dismutase) and non-enzymatic (vitamin E and glutathione) antioxidants, as well as of L-arginine applied on the scalded skin area in different combinations in the form of a lyposomal ointment on endogenous antioxidative defense components were studied both in the injured and uninjured skin. In scalded skin during hypometabolic phase, a decrease in activity of superoxide dismutase, catalase, glutathione peroxidase glutathione reductase, as well as in the level of vitamin E was observed in comparison with the control. This decrease was accompanied by a complete loss of glutathione and the activity of glutathione-S-transferase and thioredoxin reductase. The same trend of changes was recorded in hypermetabolic phase. In the uninjured skin of scalded animals, the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were at the control level both in hypo- and hypermetabolic phase. Also, no changes in vitamin E content were found while the activities of thioredoxin reductase and glutathione-S-transferase were increased. Glutathione level in this group of animals was decreased the decrease being more prominent in hyper- then in hypometabolic phase. The ointments applied to the injured parts of the skin expressed protective effects observed as an increase in vitamin E level and an attenuation of glutathione reductase activity inhibition.

scholarly journals Effect of Chronic Exposure to Pesticide Methomyl on Antioxidant Defense System in Testis of Tilapia (Oreochromis niloticus) and Its Recovery Pattern

2021 ◽  
Vol 11 (8) ◽  
pp. 3332
Author(s):  
Shunlong Meng ◽  
Xi Chen ◽  
Chao Song ◽  
Limin Fan ◽  
Liping Qiu ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Glutathione Reductase ◽  
Oreochromis Niloticus ◽  
Reduced Glutathione ◽  
Free Water ◽  
Oxidized Glutathione ◽  
Glutathione S Transferase ◽  
Recovery Pattern

The chronic effect of environmental methomyl on the antioxidant system in testis of Nile tilapia (Oreochromis niloticus) and its recovery pattern was investigated. Tilapia were exposed to sublethal concentrations of 0.2, 2, 20 and 200 μgL−1 methomyl for 30 days and thereafter moved to methomyl-free water for 18 days. Antioxidant levels in testis, including glutathione peroxidase, catalase, glutathione-S-transferase, glutathione reductase, superoxide dismutase, reduced glutathione, oxidized glutathione were measured every 6 days during the period of exposure, and at 18 days after being transferred to methomyl-free water. The results showed that lower methomyl concentration (0.2 μgL−1) had no effect on the above antioxidants, thus 0.2 μgL−1 could be seen as NOAEL for methomyl to tilapia. However, higher methomyl concentration of 2, 20 and 200 μgL−1 could significantly influence the above antioxidants. Glutathione peroxidase and oxidized glutathione increased significantly. On the contrary, reduced glutathione decreased significantly. Catalase, superoxide dismutase, glutathione reductase, glutathione-S-transferase increased at lower methomyl (2 and 20 μgL−1), but decreased at higher methomyl (200 μgL−1). The recovery test showed that oxidative damage caused by lower methomyl of 2 and 20 μgL−1 was reversible, and oxidative damage caused by higher methomyl of 200 μgL−1 was irreversible within 18 days of recovery period.

scholarly journals The distribution, induction and isoenzyme profile of glutathione S-transferase and glutathione peroxidase in isolated rat liver parenchymal, Kupffer and endothelial cells

1989 ◽  
Vol 264 (3) ◽  
pp. 737-744 ◽  
Author(s):  
P Steinberg ◽  
H Schramm ◽  
L Schladt ◽  
L W Robertson ◽  
H Thomas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Glutathione Peroxidase ◽  
Rat Liver ◽  
Peroxidase Activity ◽  
Cell Types ◽  
Transferase Activity ◽  
Glutathione Peroxidase Activity ◽  
Glutathione S Transferase ◽  
Liver Parenchymal ◽  
Parenchymal Cells

The distribution and inducibility of cytosolic glutathione S-transferase (EC 2.5.1.18) and glutathione peroxidase (EC 1.11.1.19) activities in rat liver parenchymal, Kupffer and endothelial cells were studied. In untreated rats glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene and 4-hydroxynon-2-trans-enal as substrates was 1.7-2.2-fold higher in parenchymal cells than in Kupffer and endothelial cells, whereas total, selenium-dependent and non-selenium-dependent glutathione peroxidase activities were similar in all three cell types. Glutathione S-transferase isoenzymes in parenchymal and non-parenchymal cells isolated from untreated rats were separated by chromatofocusing in an f.p.l.c. system: all glutathione S-transferase isoenzymes observed in the sinusoidal lining cells were also detected in the parenchymal cells, whereas Kupffer and endothelial cells lacked several glutathione S-transferase isoenzymes present in parenchymal cells. At 5 days after administration of Arocolor 1254 glutathione S-transferase activity was only enhanced in parenchymal cells; furthermore, selenium-dependent glutathione peroxidase activity decreased in parenchymal and non-parenchymal cells. At 13 days after a single injection of Aroclor 1254 a strong induction of glutathione S-transferase had taken place in all three cell types, whereas selenium-dependent glutathione peroxidase activity remained unchanged (endothelial cells) or was depressed (parenchymal and Kupffer cells). Hence these results clearly establish that glutathione S-transferase and glutathione peroxidase are differentially regulated in rat liver parenchymal as well as non-parenchymal cells. The presence of glutathione peroxidase and several glutathione S-transferase isoenzymes capable of detoxifying a variety of compounds in Kupffer and endothelial cells might be crucial to protect the liver from damage by potentially hepatotoxic substances.

Glutathione peroxidase, glutathione reductase, glutathione S-transferase, and γ-glutamyltranspeptidase activities in the human early pregnancy placenta

1983 ◽  
Vol 29 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Carmine Di Ilio ◽  
Giovanni Polidoro ◽  
Arduino Arduini ◽  
Antonio Muccini ◽  
Giorgio Federici
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Early Pregnancy ◽  
Glutathione S Transferase

scholarly journals Precompetitional Weight Reduction Modifies Prooxidative-Antioxidative Status in Judokas

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Katarzyna Knapik ◽  
Karolina Sieroń ◽  
Ewa Wojtyna ◽  
Grzegorz Onik ◽  
Ewa Romuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Weight Reduction ◽  
Glutathione S Transferase ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Protein Sulfhydryl ◽  
Total Oxidative Stress

Objective. The main aim of the study was an assessment of the influence of rapid weight loss on oxidative stress parameters in judokas differing in weight reduction value. Materials and Methods. The study included 30 judokas with an age range of 18-30 years (mean age: 22.4±3.40 years). Enzymatic and nonenzymatic antioxidative markers, lipid peroxidation markers, and total oxidative stress were assessed three times: one week before a competition (the first stage), after gaining the desired weight (the second stage), and one week after the competition (the third stage). Results. Between the first and the second stage, the concentration of lipid hydroperoxides (LPH) decreased significantly. The superoxide dismutase (SOD), copper- and zinc-containing superoxide dismutase (Cu,Zn-SOD), ceruloplasmin (CER), malondialdehyde (MDA), LPH, and total oxidative stress (TOS) concentrations were the lowest one week after the competition. Linear regression indicated that the emphases on increased weight reduction increased the activity of glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and protein sulfhydryl (PSH) between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD, Mn-SOD, LPH, MDA, and TOS in comparison to low and high reductions. An opposite relation was observed in PSH. In judokas, the precompetitional weight reduction range was 0.44-6.10% (mean: 2.93%±1.76%) of the initial body weight. Concentrations of superoxide dismutase (SOD; p<.01), manganese-dependent superoxide dismutase (Mn-SOD; p<.001), and ceruloplasmin (CER; p<.05) decreased between the first and the third stage of the study as well between the second and third one. Before competitions, a decrease in lipid hydroperoxide (LPH; p<.01) concentration was observed. A reduction of malondialdehyde (MDA; p<.05), LPH (p<.01), and total oxidative stress (TOS; p<.05) levels between the first and the final stage occurred. The increase in weight reduction was linearly correlated with the rise of glutathione peroxidase (GPx; p<.05), glutathione reductase (GR; p<.05), glutathione S-transferase (GST; p<.05), and protein sulfhydryl (PSH; p<.05) concentrations between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD (p<.05), Mn-SOD (p<.05), LPH (p<.05), MDA (p<.05), and TOS (p<.05) in comparison to low and high reductions. An opposite relation was observed in PSH (p<.005). Conclusions. The effect of weight reduction in judo athletes on prooxidative-antioxidative system diversity depends on the weight reduction value.

scholarly journals Comparison of oxidant and antioxidant status of seminal plasma and spermatozoa of several fish species

2013 ◽  
Vol 58 (No. 7) ◽  
pp. 313-320 ◽  
Author(s):  
A. Shaliutina-Kolešová ◽  
I. Gazo ◽  
J. Cosson ◽  
O. Linhart
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Seminal Plasma ◽  
Brook Trout ◽  
Siberian Sturgeon ◽  
Russian Sturgeon ◽  
Thiobarbituric Acid Reactive Substance ◽  
Acipenser Baerii ◽  
Carbonyl Protein

Oxidant and antioxidant activity in seminal plasma was compared with that in spermatozoa of teleost (common carp Cyprinus carpio and brook trout Salvelinus fontinalis) and chondrostean (Russian sturgeon Acipenser gueldenstaedtii, Siberian sturgeon Acipenser baerii, and sterlet Acipenser ruthenus) fishes. No differences were found between seminal plasma and spermatozoa in the level of thiobarbituric-acid-reactive substance (0.24 &plusmn; 0.08 to 0.33 &plusmn; 0.04 nmol/mg proteins) in Russian sturgeon, Siberian sturgeon, and sterlet. Carbonyl protein concentration was significantly higher in spermatozoa than in seminal plasma of all studied species. Analyzed antioxidants included superoxide dismutase, glutathione reductase, and glutathione peroxidase activity. Significant differences (P &lt; 0.05) were detected between seminal plasma and spermatozoa in total superoxide dismutase (SOD) and glutathione reductase (GR). Total glutathione peroxidase (GPx) activity was significantly higher in brook trout (12.56 &plusmn; 3.23 mU/mg proteins) and Russian sturgeon (11.56 &plusmn; 3.12 mU/mg proteins) spermatozoa compared to seminal plasma (6.81 &plusmn; 1.56 mU/mg proteins in brook trout and 9.56 &plusmn; 3.12 mU/mg proteins in Russian sturgeon). This study provides new data on oxidant and antioxidant balance between spermatozoa and seminal plasma that may be of value in the development of methods for artificial reproduction of teleost and chondrostean species. &nbsp;

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