Serological and Immunochemical Studies on the Snail (Otala lactea)

Vox Sanguinis ◽  
1968 ◽  
Vol 14 (3) ◽  
pp. 170-178
Author(s):  
H.M. Bhatia ◽  
Y. Cha Kim ◽  
W.C. Boyd
Keyword(s):  
Otala Lactea

Hemocyanin Function in Active and Dormant Land Snails, Otala lactea

1986 ◽  
Vol 59 (6) ◽  
pp. 725-732 ◽  
Author(s):  
M. Christopher Barnhart
Keyword(s):  
Land Snails ◽  
Otala Lactea

Protein synthesis in vitro by mantle tissue of the land snail Otala lactea: possible insulin-like peptide function

2000 ◽  
Vol 78 (9) ◽  
pp. 1527-1535 ◽  
Author(s):  
A M Abdraba ◽  
A SM Saleuddin
Keyword(s):  
Protein Synthesis ◽  
Cerebral Ganglion ◽  
Land Snail ◽  
Porcine Insulin ◽  
Land Snails ◽  
Size Exclusion ◽  
Cerebral Ganglia ◽  
Molecular Masses ◽  
Otala Lactea

Mantle-collar tissue from adult land snails Otala lactea continuously incorporated labelled amino acids over a 72-h period of incubation in modified culture medium. Acid-saline extract of cerebral ganglia stimulated protein synthesis by the mantle-collar tissue in vitro. This effect was dose-dependent, with the minimum and maximum doses at 0.5 and 2 cerebral ganglion equivalents, respectively. The protein synthesis-stimulating factor(s) from the cerebral ganglia appeared to be proteinaceous and hydrophobic in nature. The cerebral ganglion extract was fractionated by means of a size-exclusion HPLC column. The biological activity was induced by three fractions with estimated molecular masses of 0.82, 1.88, and 4.33 kilodaltons (kDa). Porcine insulin antiserum abolished the activity of the 4.33- and 1.88-kDa fractions but had no significant effect on the activity of the 0.82-kDa fraction. The results suggest the existence in the cerebral ganglia of more than one factor with protein synthesis-stimulating activity. One of these factors could be related to mammalian insulin. Porcine insulin, however, had no significant effect on protein synthesis by the mantle collar in vitro.

scholarly journals Pyruvate Kinase from the Land Snail Otala Lactea: Regulation by Reversible Phosphorylation During Estivation and Anoxia

1990 ◽  
Vol 154 (1) ◽  
pp. 321-337 ◽  
Author(s):  
ROSS E. WHITWAM ◽  
KENNETH B. STOREY
Keyword(s):  
Protein Kinase ◽  
Pyruvate Kinase ◽  
Time Course ◽  
Land Snail ◽  
Active Enzyme ◽  
Kinetic Properties ◽  
Reversible Phosphorylation ◽  
Enzyme Form ◽  
Otala Lactea ◽  
Activity State

Pyruvate kinase (PK) from tissues of the desert snail Otala lactea (Müller) undergoes a stable modification of its kinetic properties during estivation or in response to anoxia stress. In foot muscle and mantle, the kinetic changes induced by either state were virtually identical and were consistent with a less active enzyme form in estivation or anoxia: S0.5 PEP increased, and I50 values for Mg-ATP and L-alanine decreased, compared to the enzyme in control (aroused) snails. Estivation and anoxia also changed the properties of PK from hepatopancreas; some changes were consistent with a more active enzyme form (So.5 PEP decreased, I50 values for Mg-ATP and L-alanine increased) but the enzyme lost all sensitivity to the potent activator fructose-l,6-bisphosphate. A time course of changes in I50 Mg-ATP for foot PK and S0.5 PEP for hepatopancreas PK revealed that estivation-induced changes in enzyme properties occurred between 12 and 48 h after snails were deprived of access to food and water, whereas the reversal of these changes occurred within as little as lOmin in foot muscle after arousal was initiated. The molecular basis of the stable modification of PK kinetics appears to be reversible protein phoshorylation. The action of added cyclic-AMP-dependent protein kinase on foot or hepatopancreas PK from control (aroused) snails changed PK kinetic parameters to those characteristic of the enzyme form in estivating animals; the addition of stimulators of endogenous cyclic-GMPdependent protein kinase or protein kinase C had the same effect. Conversely, treatment with added phosphatases reconverted the properties of foot muscle PK from estivating snails to those characteristic of the control enzyme. The data suggest that reversible phosphorylation control over the activity state of regulatory enzymes of glycolysis is one mechanism contributing to the overall metabolic rate depression of the estivating state.

Polysome profiles in awake and estivating snails (Otala lactea)

1994 ◽  
Vol 108 (4) ◽  
pp. 431-436 ◽  
Author(s):  
Andrew A. Hobbs ◽  
Janet M. Attwood ◽  
Michael Guppy
Keyword(s):  
Otala Lactea
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