Red Blood Cell Antibody Screening with Groupamatic System

Vox Sanguinis ◽  
1981 ◽  
Vol 40 (1) ◽  
pp. 34-43
Author(s):  
S. Confida ◽  
C. Hurel ◽  
N. Chesnel ◽  
M. Garretto ◽  
A. Muller
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

scholarly journals Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Dietmar Enko ◽  
Claudia Habres ◽  
Franz Wallner ◽  
Barbara Mayr ◽  
Gabriele Halwachs-Baumann
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Samples ◽  
Antibody Screening ◽  
Indirect Antiglobulin Test ◽  
Cell Antibody ◽  
Antiglobulin Test ◽  
Low Ionic Strength ◽  
Enzyme Test ◽  
Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

Red Blood Cell Antibody Screening with Groupamatic System

Vox Sanguinis ◽  
1981 ◽  
Vol 40 (1) ◽  
pp. 34-43 ◽  
Author(s):  
S. Confida ◽  
C. Hurel ◽  
N. Chesnel ◽  
M. Garretta ◽  
A. Muller
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

Evaluation of a Decision Tree for Efficient Antenatal Red Blood Cell Antibody Screening

Epidemiology ◽  
2018 ◽  
Vol 29 (3) ◽  
pp. 453-457 ◽  
Author(s):  
Isabelle Le Ray ◽  
Brian Lee ◽  
Agneta Wikman ◽  
Marie Reilly
Keyword(s):  
Decision Tree ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody

scholarly journals Red blood cell antibody screening in pregnancy

2011 ◽  
Vol 5 (1) ◽  
pp. 56 ◽  
Author(s):  
ShanthalaA.M Devi ◽  
VanamalaA Alwar ◽  
S Sitalakshmi ◽  
Karuna Rameshkumar ◽  
Rita Mhaskar
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Antibody Screening ◽  
Cell Antibody ◽  
In Pregnancy

Unexpected red blood cell antibody distributions in Chinese people by a systematic literature review

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 975-979 ◽  
Author(s):  
Chunxia Chen ◽  
Jinzhe Tan ◽  
Lixin Wang ◽  
Bing Han ◽  
Wei Sun ◽  
...  
Keyword(s):  
Literature Review ◽  
Blood Cell ◽  
Systematic Literature Review ◽  
Red Blood Cell ◽  
Chinese People ◽  
Cell Antibody

scholarly journals Red blood cell alloimmunisation and autoimmunisation in transfusion dependent beta thalassemics from Southern India

2015 ◽  
Vol 05 (03) ◽  
pp. 004-008
Author(s):  
Mohammed Saleem E. K. ◽  
Soundarya Mahalingam ◽  
Shamee Shastri ◽  
Kamalakshi G. Bhat
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Beta Thalassemia ◽  
Red Cell ◽  
Transfusion Therapy ◽  
Female Ratio ◽  
Antibody Screening ◽  
Cell Antigen ◽  
Low Ionic Strength ◽  
Male To Female

AbstractThe development of red blood cell (RBC) isoimmunization with alloantibodies and autoantibodies complicate transfusion therapy in multiply transfused thalassemia patients. We conducted a study to analyse the frequency in our population. Clinical and antibody profile from 55 multiply transfused thalassemic patients who were receiving transfusions were collected and analyzed prospectively. A commercially available 3 cell antigen panel was used for the antibody screening procedure. If antibody screening with the 3-cell antigen panel was positive, an extended 11-cell antigen panel was used for antibody identification in LISS (Low Ionic Strength Solution). All patients received blood matched for only ABO and Rh (D) antigens. A total of 55 transfusion dependent â thalassemics were included in this study out of which 30 (54.55%) were males and 25(45.45%) females with a male to female ratio of 1.2: 1. Frequency of red cell alloimmunization in this study was found to be 1.8%. None of the patients developed red cell autoimmunization. The alloantibody identified in the the patient who developed alloimmunisation was was anti-K. In conclusion, the transfusion of matched blood is essential for chronically transfused beta thalassemia patients in order to avoid alloimmunization.

scholarly journals Prevalence and Specificity of RBC Alloantibodies in Indian Patients Attending a Tertiary Care Hospital

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Shamsuz Zaman ◽  
Rahul Chaurasia ◽  
Kabita Chatterjee ◽  
Rakesh Mohan Thapliyal
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Patient Population ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
North India ◽  
Care Hospital ◽  
Antibody Screening ◽  
Resource Limited ◽  
Transfusion Requirements

Background.Red blood cell (RBC) alloimmunization results from genetic disparity of RBC antigens between donor and recipients. Data about alloimmunization rate in general patient population is scarce especially from resource limited countries. We undertook this study to determine prevalence and specificity of RBC alloantibodies in patients admitted in various clinical specialties at a tertiary care hospital in North India.Methods.Antibody screening was carried out in 11,235 patients on automated QWALYS 3 platform (Diagast, Loos, France). Antibody identification was carried out with an 11-cell identification panel (ID-Diapanel, Diamed GmbH, Switzerland).Results.The overall incidence of RBC alloimmunization in transfused patients was 1.4% (157/11235), with anti-E being the most common specificity (36.3%), followed by anti-D (16%), anti-c (6.4%), anti-c + E (6.4%), anti-C + D (5.1%), and anti-K (4.5%). The highest incidence of alloimmunization was observed in hematology/oncology patients (1.9%), whereas in other specialties the range was 0.7–1%.Conclusion.As alloimmunization complicates the transfusion outcomes, authors recommend pretransfusion antibody screening and issue of Rh and Kell matched blood to patients who warrant high transfusion requirements in future.

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