Human Cytomegalovirus DNA is not Detectable with Nested Double Polymerase Chain Reaction in Healthy Blood Donors

Vox Sanguinis ◽  
1995 ◽  
Vol 68 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Noriko Urushibara ◽  
Kil-Won Kwon ◽  
Tsuneo A. Takahashi ◽  
Sadayoshi Sekiguchi
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Blood Donors ◽  
Chain Reaction ◽  
Polymerase Chain

Human Cytomegalovirus DNA Is Not Detectable with Nested Double Polymerase Chain Reaction in Healthy Blood Donors

Vox Sanguinis ◽  
1995 ◽  
Vol 68 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Noriko Urushibara ◽  
Kil-Won Kwon ◽  
Tsuneo A. Takahashi ◽  
Sadayoshi Sekiguchi
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Blood Donors ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of human cytomegalovirus DNA in peripheral blood leukocytes by the polymerase chain reaction

Transfusion ◽  
2003 ◽  
Vol 33 (9) ◽  
pp. 783-784 ◽  
Author(s):  
Ian.S. Bevan ◽  
Matthew R. Walker ◽  
Richard A. Daw ◽  
Andreas Bitsch ◽  
Gregor Bein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Polymerase Chain

Molecular Detection of Transfusion Transmitted Virus Coinfection With Some Hepatotropic Viruses

2006 ◽  
Vol 130 (11) ◽  
pp. 1680-1683
Author(s):  
Maysaa El-Sayed Zaki ◽  
Nagy Abd El Hady, MD
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Hepatitis ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Blood Donors ◽  
Unknown Etiology ◽  
Chain Reaction ◽  
Transmitted Virus ◽  
Polymerase Chain ◽  
Transfusion Transmitted Virus

Abstract Context.—A novel human DNA virus was isolated from the serum of a patient with posttransfusion hepatitis; it was named transfusion transmitted virus (TTV). Objective.—To ascertain the influence of TTV (detected by polymerase chain reaction amplification of a conserved region of the viral genome) coinfection in individuals infected with hepatitis viruses (A, B, and C) and to investigate the putative role played by TTV in hepatic dysfunction in individuals with acute non-A-E hepatitis. Design.—Sixty-two patients with viral hepatitis were included in the study in addition to 18 blood donors. Viral study of 4 hepatotropic viruses (A, B, C, and E) was carried out. Study for TTV DNA was performed by nested polymerase chain reaction. Results.—The prevalence of TTV was not statistically different between hepatitis patients and blood donors, and it was not correlated to the levels of the hepatic aspartate aminotransferase and alanine aminotransferase between individuals evidencing dual infection with hepatitis B and C viruses and healthy blood donors. However, in the group of patients with viral hepatitis of unknown etiology (non-A-E), those evidencing TTV viremia had statistically significant lower levels of alanine aminotransferase (P = .03) and aspartate aminotransferase (P = .04) than those who were TTV negative. Conclusions.—We can conclude that TTV is a frequent virus isolated from patients with various types of viral hepatitis, from cases of hepatitis without obvious viral agent, and from the healthy population. TTV has no effect on biochemical markers of associated viral hepatitis. It may be associated with a mild form of non-A-E hepatitis.

scholarly journals Introduction of NAT-testing for infections for screening blood donors in Republic of Kazakhstan

2015 ◽  
Vol 96 (3) ◽  
pp. 414-417
Author(s):  
T N Savchuk ◽  
Z K Burkitbaev ◽  
S A Abdrakhmanova ◽  
S V Skorikova ◽  
N S Kuz’min
Keyword(s):  
Human Immunodeficiency Virus ◽  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Blood Donors ◽  
Chain Reaction ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
Polymerase Chain

Aim. To evaluate the effectiveness of NAT-screening (nucleic acid amplification technologies) for infections in blood donors in Kazakhstan. Methods. Statistical data of blood donors screening examinations in the Republic of Kazakhstan in 2012-2014 were evaluated. Results. In 2014, the number of examined donors increased by 3.4% compared with 2012. The number of deferrals due to positive screening results for serological markers decreased by 10.9%, while the share of such donors decreased by 13.8% [p <0.01; odds ratio (OR) - 0.86, 95% confidence interval (CI 95%) - 0.83-0.88); χ2=136.76]. In 2014, 100% of donations were screened using NAT-testing (312,510 donors). Most of the NAT-screening in Kazakhstan is performed using closed automated systems. In 2012, 1 Blood Center conducted a polymerase chain reaction screening by open circuit polymerase chain reaction systems, in 5 blood centers polymerase chain reaction was performed with manual sample preparation. In 2014, the number of deferrals due to positive NAT-testing results has increased by 44.3%, the share of such donors - by 38.7% (p <0.01; OR=1.39, 95% CI=1.15-1.67); χ2=11.82). Seronegative NAT-positive samples were discovered according to the results of discriminant test, including human immunodeficiency virus - 2 (0.8%) samples, hepatitis B virus -182 (73.4%), hepatitis C virus - 60 (24.2%), negative result - 4 (1.6%). Conclusion. The introduction of screening NAT-testing of donated blood prevented transfusion of blood infected with: human immunodeficiency virus - 1 in 150,000 donations, hepatitis B virus - 1 in 1.650 donations, hepatitis C virus - 1 in 5,000 donations.

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