Demonstration by Flow Cytometry of the Numbers of Residual White Blood Cells and Platelets in Filtered Red Blood Cell Concentrates and Plasma Preparations

Vox Sanguinis ◽  
1997 ◽  
Vol 73 (4) ◽  
pp. 220-229 ◽  
Author(s):  
J. Neumüller ◽  
D.W.M. Schwartz ◽  
W.R. Mayr
Keyword(s):  
Flow Cytometry ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
White Blood Cells

scholarly journals PO-283 Correlation Analysis between Blood Cell Viability, Five Elements and Competition Performance of Elite Men’s Rowers

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Junbei Bai
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Aerobic Capacity ◽  
Red Blood Cell ◽  
Iron Content ◽  
Blood Cells ◽  
White Blood Cells ◽  
Cell Activity ◽  
Ordinary People ◽  
Five Elements

Objective To observe the national elite male rowers blood, red blood cell activity and serum copper, zinc, calcium, magnesium and iron content of the five elements, and compared with the ordinary people. Aimed to investigate the between athletes, athletes and ordinary differences between the two sets of indicators and to explore the impact of element contents in red blood cell activity and five factors. Trying to bring two sets of indicators and specific combining ability, used in training on the monitoring function, and for the future to provide some references for further study. Methods It was included 22 athletes and 22 ordinary men, as the research object, in the collection of blood, measuring red blood cell activity in the blood content of the five elements, simultaneous measurement of physical indicators , will be doing all the data at the differences between the two groups compared to the group to do correlation analysis. The recent record of 2000m, 6000m rowing Dynamometer test results, and red blood cell activity associated with the five elements of content analysis. Results 1. Athletes indicators related to aerobic exercise were significantly higher than ordinary people. The white blood cells of athletes group were average.It shows that athletes have high aerobic capacity, while white blood cells are more stable than normal people. The members of the national rowing men's iron, magnesium content was significantly higher than ordinary group, the iron content is higher than the normal reference value; blood calcium levels were significantly lower than ordinary people, and lower than the normal reference value. The total number of red blood cells and the number of living cells was very significant positive correlation in two groups subjects; Red blood cell activity and red blood cell diameter is proportional, and red blood cell roundness in inverse proportion to the relationship; from this experiment a special ability to see red blood cell activity and there is no correlation. In both groups, hemoglobin was positively correlated with iron content, while iron was positively correlated with copper content. Conclusions 1. Increasing the number and volume of red blood cells can effectively increase the activity of red blood cells; red blood cell activity has no correlation with specific ability, and can not be used as an indicator to determine specific ability. The content of iron and magnesium in rowers is higher than that in ordinary people, which indicates that the adjustment of aerobic capacity and nerve control is very effective. The lower calcium content indicates that the injury caused by calcium loss should be prevented and the urgency of calcium supplementation should be emphasized. In training, we should pay attention to increasing hemoglobin content and aerobic capacity by supplementing iron. We can further consider the effect of supplementing copper to promote iron supplementation.

Perturbation of red blood cell flow in small tubes by white blood cells

1989 ◽  
Vol 413 (4) ◽  
pp. 372-377 ◽  
Author(s):  
Thomas Neil Thompson ◽  
Paul L. La Celle ◽  
Giles R. Cokelet
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
White Blood Cells

scholarly journals Hematological changes of Anemia`s in Horses of Equestrian club in Baghdad

2012 ◽  
Vol 36 (0A) ◽  
pp. 92-97
Author(s):  
Faisal G. Habasha
Keyword(s):  
Blood Cell ◽  
Sedimentation Rate ◽  
Red Blood Cell ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Samples ◽  
Erythrocyte Sedimentation ◽  
Blood Smears ◽  
Hematological Changes

This study was conducted to know thehematological changes of anemia in horsesat equestrian club in Baghdad. Blood samples were collected from 151 horses of both sexes(74 male and 77 female) and different agesrandomly. The study includedred blood cells count, white blood cells, hemoglobin, packed cell volume and differential blood smears, togetherwith erythrocyte sedimentation rate readings. The study showed increased white blood cells count mainly neutrophilwith decreased hemoglobinand red blood cell countin addition to erythrocyte sedimentation rate.The blood smears showeddifferent changes of red blood cell.

scholarly journals Rapid Cl−/HCO3−exchange kinetics of AE1 in HEK293 cells and hereditary stomatocytosis red blood cells

2013 ◽  
Vol 305 (6) ◽  
pp. C654-C662 ◽  
Author(s):  
Etienne Frumence ◽  
Sandrine Genetet ◽  
Pierre Ripoche ◽  
Achille Iolascon ◽  
Immacolata Andolfo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Anion Exchange ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Hek293 Cells ◽  
Single Amino Acid ◽  
Stopped Flow ◽  
Wild Type

Anion exchanger 1 (AE1) or band 3 is a membrane protein responsible for the rapid exchange of chloride for bicarbonate across the red blood cell membrane. Nine mutations leading to single amino-acid substitutions in the transmembrane domain of AE1 are associated with dominant hereditary stomatocytosis, monovalent cation leaks, and reduced anion exchange activity. We set up a stopped-flow spectrofluorometry assay coupled with flow cytometry to investigate the anion transport and membrane expression characteristics of wild-type recombinant AE1 in HEK293 cells, using an inducible expression system. Likewise, study of three stomatocytosis-associated mutations (R730C, E758K, and G796R), allowed the validation of our method. Measurement of the rapid and specific chloride/bicarbonate exchange by surface expressed AE1 showed that E758K mutant was fully active compared with wild-type (WT) AE1, whereas R730C and G796R mutants were inactive, reinforcing previously reported data on other experimental models. Stopped-flow analysis of AE1 transport activity in red blood cell ghost preparations revealed a 50% reduction of G796R compared with WT AE1 corresponding to a loss of function of the G796R mutated protein, in accordance with the heterozygous status of the AE1 variant patients. In conclusion, stopped-flow led to measurement of rapid transport kinetics using the natural substrate for AE1 and, conjugated with flow cytometry, allowed a reliable correlation of chloride/bicarbonate exchange to surface expression of AE1, both in recombinant cells and ghosts and therefore a fine comparison of function between different stomatocytosis samples. This technical approach thus provides significant improvements in anion exchange analysis in red blood cells.

Lithium Levels in White Blood Cells and their Relationship to Observed Side Effects

1983 ◽  
Vol 143 (1) ◽  
pp. 36-39 ◽  
Author(s):  
Yong Lock Ong
Keyword(s):  
Side Effects ◽  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Strong Correlation ◽  
Blood Cells ◽  
Side Effect ◽  
White Blood Cells ◽  
Lithium Therapy ◽  
Hand Tremor

SummaryA strong correlation was obtained between white blood cell (WBC) lithium concentrations and the severity of observed side effects in a group of 40 patients receiving prophylactic lithium therapy. However, there was no significant correlation between these levels and the specific side effect of hand tremor, although WBC concentrations were higher in patients with greater tremor. These results contrasted with those for plasma and red blood cell (RBC) lithium concentrations, which showed no relationship to side effects. This suggests that WBC lithium concentrations may be a more sensitive index of side effects than conventional plasma estimations.

scholarly journals Effects of aqueous extract of Nelsonia canescens leaf on the osmotic fragility of red blood cell and blood parameters of Wistar albino rats.

2018 ◽  
Vol 1 (1) ◽  
pp. 1-10
Author(s):  
G.M. Oladele
Keyword(s):  
Blood Cell ◽  
Aqueous Extract ◽  
Red Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Osmotic Fragility ◽  
Albino Rats ◽  
Hypotonic Solution ◽  
Dependent Manner ◽  
Immune Booster

The herbaceous plant Nelsonia canescens is a medicinal plant used in Asian and African traditional medicine for various diseases of humans and animals. The plant has been used for the treatment of pains and inflammatory action related diseases, cancer, gout, cough, fever, cardiovascular diseases, chicken pox and even malaria. Decoction of it has also been used as immune booster in patients by the traditionalists. This study was aimed at evaluating the effects of the aqueous leaf extract of the plant on the osmotic fragility of rats’ red blood cells, and also to determine the changes that occur in haematology and serum chemistry of the rats exposed orally to the extract for 28 days. Three groups of rats were administered orally with 200, 400, and 800mg/kg of the plant extract respectively while the fourth group which is the control was administered also orally with distilled water and their blood were then analyzed. The 2, 4 and 6mg/ml concentrations of the extract inhibit hypotonic solution induced rats erythrocytes hemolysis in concentration dependent manner and the inhibition is comparable to that of Indomethacin. The blood analysis showed a significant increase (p<0.05) in total white blood cells and the lymphocytes for the groups administered with 400 and 800 mg/kg of the extract while the neutrophils decreased significantly. It was then concluded that the aqueous extract of the plant inhibits red blood cell hemolysis and hence its anti-inflammatory activities. Also, the significant increase in the total white blood cells and the lymphocytes could be the reason why the plant is useful as immune booster by the traditionalists.

scholarly journals Impact of Cypermethrin 25% EC and pH on Certain Haematological Parameters of Cyprinid Fish Puntius javanicus

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Roy Tushar Kanti ◽  
Saha Samir Kumar
Keyword(s):  
Blood Glucose ◽  
Blood Cell ◽  
Blood Glucose Level ◽  
Red Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Lethal Concentration ◽  
Total Serum Protein ◽  
Total Serum

In present study an attempt has been made to investigate the combined impact of sub lethal toxicity of Cypermethrin 25% EC and pH on certain blood parameters of Puntius javanicus. Adult fish were exposed to sublethal concentration (0.36 µg/L) of cypermethrin 25% EC in different pH water i.e. pH 5, pH 6, pH 7, pH 8.5 and pH 9.5 for 15 days. Significant changes in red blood cell (RBCs) count, total white blood cells (WBCs), haemoglobin % (Hb%), haematocrit value, total serum protein and blood glucose level were observed in fish exposed to sub-lethal concentration of cypermethrin. Significant decrease in red blood cell (RBCs) count, haemoglobin % (Hb %), haematocrit value and total serum protein content were found in fish exposed to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. A significant increase in these parameters were documented in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. On the other hand a significant increase of total white blood cells (WBCs) and blood glucose level were recorded in fish subjected to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. Values of these parameters were decreased significantly in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. The values of all studied blood parameters in fish subjected to sub-lethal concentration of cypermethrin in pH 8.5 were almost found to retain the values of control group (pH 7, without toxicant).

scholarly journals Вплив «Трифузолу» на загоєння післякастраційних ран у кнурів

2013 ◽  
pp. 77-80
Author(s):  
С. М. Кулинич
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Size Distribution ◽  
Red Blood Cell ◽  
Postoperative Period ◽  
Blood Cells ◽  
Single Injection ◽  
White Blood Cells ◽  
Morphological Studies

За динамікою морфологічних досліджень крові представлені результати ефективності внутріш-ньоорбітальних вливань 1,0 % розчину «Трифузолу» в дозі 5 мл на одну тварину. Встановлено, що після одноразового введення препарату в післяопераційний період на сьому добу реєструється зменшення кіль-кості лейкоцитів, що поєднується зі зростанням у лейкограмі ґранулоцитів. Зміни в складі клітин чер-воної крові, характеризуються зниженням кількості еритроцитів на 9,4 %, а також на 12,1 % такого показника, як розподілення розміру еритроцитів. According to the dynamics of morphological studies, the findings of the effectiveness of blood internal orbital injections of 1.0% solution “Tryfuzol” in a dose of 5 ml per animal were presented. It was found that after a single injection of the drug in the postoperative period, on the seventh day the reduction of the number of white blood cells is recorded, that is combined with the growth of granulocytes in leucogram. Changes in red blood cells are characterized by the decrease of red blood cell by 9.4% and 12.1% of the index of the size distribution of red blood cells.

scholarly journals Transforming growth factor-β released by apoptotic white blood cells during red blood cell storage promotes transfusion-induced alloimmunomodulation

Transfusion ◽  
2015 ◽  
Vol 55 (7) ◽  
pp. 1721-1735 ◽  
Author(s):  
Romain Vallion ◽  
Francis Bonnefoy ◽  
Anna Daoui ◽  
Loredane Vieille ◽  
Pierre Tiberghien ◽  
...  
Keyword(s):  
Growth Factor ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Transforming Growth Factor ◽  
White Blood Cells ◽  
Transforming Growth Factor Β ◽  
Cell Storage
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