Role of Peroxide in the Stimulation of the Hexose Monophosphate Shunt during Phagocytosis by Polymorphonuclear Leukocytes

Enzyme ◽  
1972 ◽  
Vol 13 (1-3) ◽  
pp. 110-131 ◽  
Author(s):  
J. Noseworthy Jr. ◽  
M.L. Karnovsky
Keyword(s):  
Polymorphonuclear Leukocytes ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Shunt ◽  
Stimulation Of

scholarly journals The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leukocytes.

1976 ◽  
Vol 143 (6) ◽  
pp. 1308-1316 ◽  
Author(s):  
M Tsan ◽  
P A McIntyre
Keyword(s):  
Sialic Acid ◽  
Concanavalin A ◽  
Oxidative Metabolism ◽  
Polymorphonuclear Leukocytes ◽  
Superoxide Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

The effect of desialylation on phagocytosis of latex particles and oxidative metabolism of human polymorphonuclear leukocytes was studied. Removal of 20% total leukocyte sialic acid by bacterial neuraminidase had no effect on phagocytosis of latex particles and phagocytosis-associated activation of hexose monophosphate shunt in human polymorphonuclear leukocytes. In contrast, desialylation prevented the stimulation of superoxide production either by phagocytosis or by concanavalin A. It is concluded that membrane sialic acid is essential for the stimulation of superoxide production by human polymorphonuclear leukocytes.

scholarly journals Stimulation of the hexose monophosphate shunt independent of hydrogen peroxide and superoxide production in rabbit alveolar macrophages during phagocytosis

Blood ◽  
1977 ◽  
Vol 50 (5) ◽  
pp. 935-945 ◽  
Author(s):  
MF Tsan
Keyword(s):  
Alveolar Macrophages ◽  
Polymorphonuclear Leukocytes ◽  
Light Emission ◽  
Cell Types ◽  
H2o2 Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Abstract Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter) per cell. There was stimulation of O2- and H2O2 production, light emission, and activation of the hexose monophosphate shunt during phagocytosis by human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter) per cell. There was no stimulation of the production of O2- and H2O2 or light emission associated with phagocytosis by rabbit AM, while the hexose monophosphate shunt was activated. Similar metabolic changes were obtained in both cell types when opsonized zymosan was used as phagocytic particles. 1-14C- glucoseoxidation was stimulated by H2O2 and methylene blue in both resting human PMN and rabbit AM. It is concluded that activation of the hexose monophosphate shunt in rabbit AM during phagocytosis is independent of O2- and H2O2 production.

scholarly journals Stimulation of the hexose monophosphate shunt independent of hydrogen peroxide and superoxide production in rabbit alveolar macrophages during phagocytosis

Blood ◽  
1977 ◽  
Vol 50 (5) ◽  
pp. 935-945
Author(s):  
MF Tsan
Keyword(s):  
Alveolar Macrophages ◽  
Polymorphonuclear Leukocytes ◽  
Light Emission ◽  
Cell Types ◽  
H2o2 Production ◽  
Hexose Monophosphate ◽  
Latex Particles ◽  
Hexose Monophosphate Shunt ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of

Phagocytosis and oxidative metabolism of human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM) were studied. Human PMN ingested a mean of 12 polyvinyl toluene latex particles (2 micrometer in diameter) per cell. There was stimulation of O2- and H2O2 production, light emission, and activation of the hexose monophosphate shunt during phagocytosis by human PMN. Rabbit AM ingested 51 latex particles (2 micrometer in diameter) per cell. There was no stimulation of the production of O2- and H2O2 or light emission associated with phagocytosis by rabbit AM, while the hexose monophosphate shunt was activated. Similar metabolic changes were obtained in both cell types when opsonized zymosan was used as phagocytic particles. 1-14C- glucoseoxidation was stimulated by H2O2 and methylene blue in both resting human PMN and rabbit AM. It is concluded that activation of the hexose monophosphate shunt in rabbit AM during phagocytosis is independent of O2- and H2O2 production.

scholarly journals The response of red cell hexose monophosphate shunt after sulfhydryl inhibition

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 49-54 ◽  
Author(s):  
AL Jr Sagone ◽  
SP Balcerzak ◽  
EN Metz
Keyword(s):  
Carbon Monoxide ◽  
Ionization Chamber ◽  
Human Erythrocytes ◽  
Red Cells ◽  
Red Cell ◽  
Hexose Monophosphate ◽  
Peroxide Formation ◽  
Hexose Monophosphate Shunt ◽  
Vibrating Reed ◽  
Stimulation Of

Abstract In this investigation, we studied the importance of cellular glutathione (GSH) in the hexose monophosphate shunt (HMPS) activity of unstimulated human erythrocytes and the mechanism by which pyruvate stimulates the HMPS. The rate of HMPS activity was measured by the production of radioactive CO2 from 14C-1-glucose or 14C-1-ribose using a vibrating reed electrometer and ionization chamber. HMPS activity was not significantly impaired by N-ethylmaleimide (NEM) in concentrations which bound all red cell GSH. Red cells incubated under carbon monoxide (CO), an experimental condition which eliminates peroxide production, still had HMPS activity which was 44% of the value under air. Pyruvate stimulation of the HMPS was unaffected by doses of NEM which bound all cellular GSH or by incubation under CO. These data indicated that pyruvate stimulation of the HMPS occurs by pathways which do not involve peroxide formation, GSH, or oxygen. This study indicates that sulfhydrylblockade of GSH does not necessarily inhibit HMPS activity and that HMPS activity in red cells may respond to reactions not linked directly to glutathione reduction.

The role of transmembrane cationic gradients in immune complex stimulation of human polymorphonuclear leukocytes

1988 ◽  
Vol 134 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Francis W. Luscinskas ◽  
Deiren E. Mark ◽  
Beatrice Brunkhorst ◽  
Fabian J. Lionetti ◽  
Edward J. Cragoe ◽  
...  
Keyword(s):  
Immune Complex ◽  
Polymorphonuclear Leukocytes ◽  
Human Polymorphonuclear Leukocytes ◽  
Stimulation Of
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