Mechanism of Stimulation of the Hexose Monophosphate Shunt of Erythrocytes by Pyruvate

Enzyme ◽  
1974 ◽  
Vol 18 (1-2) ◽  
pp. 7-18 ◽  
Author(s):  
Ernest Beutler ◽  
Enriqueta Guinto
Keyword(s):  
Hexose Monophosphate ◽  
Hexose Monophosphate Shunt ◽  
Stimulation Of

scholarly journals The response of red cell hexose monophosphate shunt after sulfhydryl inhibition

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 49-54 ◽  
Author(s):  
AL Jr Sagone ◽  
SP Balcerzak ◽  
EN Metz
Keyword(s):  
Carbon Monoxide ◽  
Ionization Chamber ◽  
Human Erythrocytes ◽  
Red Cells ◽  
Red Cell ◽  
Hexose Monophosphate ◽  
Peroxide Formation ◽  
Hexose Monophosphate Shunt ◽  
Vibrating Reed ◽  
Stimulation Of

Abstract In this investigation, we studied the importance of cellular glutathione (GSH) in the hexose monophosphate shunt (HMPS) activity of unstimulated human erythrocytes and the mechanism by which pyruvate stimulates the HMPS. The rate of HMPS activity was measured by the production of radioactive CO2 from 14C-1-glucose or 14C-1-ribose using a vibrating reed electrometer and ionization chamber. HMPS activity was not significantly impaired by N-ethylmaleimide (NEM) in concentrations which bound all red cell GSH. Red cells incubated under carbon monoxide (CO), an experimental condition which eliminates peroxide production, still had HMPS activity which was 44% of the value under air. Pyruvate stimulation of the HMPS was unaffected by doses of NEM which bound all cellular GSH or by incubation under CO. These data indicated that pyruvate stimulation of the HMPS occurs by pathways which do not involve peroxide formation, GSH, or oxygen. This study indicates that sulfhydrylblockade of GSH does not necessarily inhibit HMPS activity and that HMPS activity in red cells may respond to reactions not linked directly to glutathione reduction.

Stimulation of neutrophil chemotaxis, adhesiveness, phagocytosis, and hexose monophosphate shunt activity by N-(2-mercaptopropionyl) glycine

1981 ◽  
Vol 178 (3) ◽  
pp. 257-262 ◽  
Author(s):  
Franco Patrone ◽  
Franco Dallegri ◽  
Anna Maria Maggi ◽  
Claudio Rapetto ◽  
Gianfranco Lanzi
Keyword(s):  
Hexose Monophosphate ◽  
Neutrophil Chemotaxis ◽  
Hexose Monophosphate Shunt ◽  
Stimulation Of
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