Rhodanese Activity during the Embryonic Development of Mouse Liver and Kidney

Enzyme ◽  
1975 ◽  
Vol 20 (3) ◽  
pp. 138-150 ◽  
Author(s):  
Brian R. Unsworth
Keyword(s):  
Embryonic Development ◽  
Mouse Liver ◽  
Liver And Kidney

Organ-specific gene masking in mammalian chromosomes

1970 ◽  
Vol 176 (1044) ◽  
pp. 277-285 ◽  
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Dna Hybridization ◽  
Mouse Liver ◽  
Early Event ◽  
Specific Gene ◽  
Histone Proteins ◽  
Organ Specific ◽  
Liver And Kidney

Chromatin (chromosomal nucleoprotein) from mammalian colls is used as a template for the synthesis of RNA which is characterized and compared with other RNA by RNA-DNA hybridization. It is found to be transcribed from a restricted set of sequences and cannot be distinguished from natural RNA from the same organ as the chromatin. In contrast, it is different from RNA from other organs. Hence, DNA is masked in an organ-specific way in vivo and the masking is preserved on isolation. When cell division is induced in mouse liver and kidney a very early event is a change in masking in chromatin. This precedes changes in RNA populations; both precede DNA synthesis. Chromatin can be accurately reconstructed from DNA, histones and non-histone proteins. Experiments using this system indicate that histones non-specifically mask DNA; non-histone proteins are essential to reverse masking in a specific way.

Effect of the mycotoxin citrinin on composition of mouse liver and kidney

Toxicon ◽  
1978 ◽  
Vol 16 (4) ◽  
pp. 351-359 ◽  
Author(s):  
Richard D. Phillips ◽  
A.Wallace Hayes
Keyword(s):  
Mouse Liver ◽  
Liver And Kidney

scholarly journals Comparative Effects of Phosphoenolpyruvate, a Glycolytic Intermediate, as an Organ Preservation Agent with Glucose and N-Acetylcysteine against Organ Damage during Cold Storage of Mouse Liver and Kidney

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Yoichi Ishitsuka ◽  
Yusuke Fukumoto ◽  
Yuki Kondo ◽  
Mitsuru Irikura ◽  
Daisuke Kadowaki ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Organ Preservation ◽  
Mouse Liver ◽  
Organ Damage ◽  
Histological Changes ◽  
Glycolytic Intermediate ◽  
Cold Preservation ◽  
Oxidative Stress Parameters ◽  
Liver And Kidney ◽  
And Oxidative Stress

We evaluated the usefulness of phosphoenolpyruvate (PEP), a glycolytic intermediate with antioxidative and energy supplementation potentials, as an organ preservation agent. Using ex vivo mouse liver and kidney of a static cold storage model, we compared the effects of PEP against organ damage and oxidative stress during cold preservation with those of glucose or N-acetylcysteine (NAC). Lactate dehydrogenase (LDH) leakage, histological changes, and oxidative stress parameters (measured as thiobarbituric acid reactive substance and glutathione content) were determined. PEP (100 mM) significantly prevented an increase in LDH leakage, histological changes, such as tubulonecrosis and vacuolization, and changes in oxidative stress parameters during 72 h of cold preservation in mouse liver. Although glucose (100 mM) partly prevented LDH leakage and histological changes, no effects against oxidative stress were observed. By contrast, NAC inhibited oxidative stress in the liver and did not prevent LDH leakage or histological changes. PEP also significantly prevented kidney damage during cold preservation in a dose-dependent manner, and the protective effects were superior to those of glucose and NAC. We suggest that PEP, a functional carbohydrate with organ protective and antioxidative activities, may be useful as an organ preservation agent in clinical transplantation.

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