scholarly journals Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

2017 ◽  
Vol 41 (2) ◽  
pp. 722-730 ◽  
Author(s):  
Adriana Mika ◽  
Jaroslaw Kobiela ◽  
Aleksandra Czumaj ◽  
Michał Chmielewski ◽  
Piotr Stepnowski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tissue Expression ◽  
Gas Chromatography Mass Spectrometry ◽  
Cancer Tissue ◽  
Colon Mucosa ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Mucosa ◽  
Metabolic Marker ◽  
Cerotic Acid

Backgrounds/Aims: Colorectal cancer (CRC) cells show some alterations of lipid metabolism. Elongation of fatty acids (FA) has not been studied in CRC tissues thus far. The aim of this study was to verify if CRC specimens and normal colon mucosa differ in terms of their levels of very long-chain FAs, a product of FA elongation. Moreover, the expression of elongase genes has been studied in normal tissue and CRC. Finally, we searched for some specific products of FA elongation in serum of CRC patients. Methods: The specimens of normal colon mucosa and CRC were obtained from nineteen CRC patients differ in terms of FA elongation. We also searched for some specific products of FA elongation in serum of CRC patients and from healthy volunteers. Tissue and serum FA profiles were determined by means of gas chromatography-mass spectrometry (GC/MS), and the tissue expression of elongases (ELOVLs) was analyzed with real-time PCR. Results: Compared to normal colon tissue, CRC specimens showed significantly higher levels of 22-, 24- and 26-carbon FAs, stronger expressions of ELOVL1 and ELOVL6 (4- and 9-fold elevated respectively), and higher values of 18: 0/16: 0 elongation index. We also demonstrated presence of cerotic acid (26: 0) in serum of all CRC patients but in none of the healthy controls. Conclusions: CRC tissue seems to be characterized by enhanced FA elongation (hyper-elongation). Presence of cerotic acid in CRC patients sera and absence of this FA in healthy subjects points to this compound as a strong candidate for specific metabolic marker of colorectal malignancies.

New insights to the clinical implication of HOXA5 as prognostic biomarker in patients with colorectal cancer

2020 ◽  
pp. 1-9
Author(s):  
Hamza Yaiche ◽  
Haifa Tounsi-Kettiti ◽  
Nadia Ben Jemii ◽  
Amira Jaballah Gabteni ◽  
Najla Mezghanni ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Endothelial Cells ◽  
Tumor Cells ◽  
Control Group ◽  
Colon Mucosa ◽  
Normal Colon ◽  
Cytoplasmic Staining ◽  
Normal Colon Mucosa ◽  
Abnormal Expression ◽  
Potential Biomarker

BACKGROUND: Homeobox A5 (HOXA5) is a member of the HOX protein family which is involved in several pathways of carcinogenesis, and is dysregulated in many types of cancers. However, its expression and function in human colorectal cancer (CRC) is still largely unknown. OBJECTIVE: This study, aimed to evaluate HOXA5 expression in Tunisian patients with CRC in order to define new potential biomarker. METHODS: An immunohistochemical study using an HOXA5 antibody was performed on 85 formalin fixed paraffin embedded specimens from patients with CRC. Six normal colon mucosa cases were used as controls. RESULTS: HOXA5 expression showed a cytoplasmic staining in both tumor and stromal/endothelial cells. Loss or low HOXA5 expression was seen in tumor cells in 74/85 cases (87.06%) and in stromal/endothelial cells, in 77/85 (90.59%). In control group of normal colon mucosa HOXA5 was moderately expressed in all the cases. The abnormal expression, was significantly associated to lymph nodes metastasis in tumor cells (p= 0.043) and in stromal/endothelial cells (p= 0.024). CONCLUSION: In our series, HOXA5 immunostaining suggests the valuable role of this protein in colorectal carcinogenesis. Moreover, the association of lymph node metastasis to HOXA5 abnormal expression underlies its crucial role in colorectal cancer dissemination and prognosis.

scholarly journals Mast cells in adjacent normal colon mucosa rather than those in invasive margin are related to progression of colon cancer

2011 ◽  
Vol 23 (4) ◽  
pp. 276-282 ◽  
Author(s):  
Qing Xia ◽  
Ya Ding ◽  
Xiao-jun Wu ◽  
Rui-qing Peng ◽  
Qiang Zhou ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Mast Cells ◽  
Colon Mucosa ◽  
Normal Colon ◽  
Invasive Margin ◽  
Normal Colon Mucosa

scholarly journals LINE-1 hypomethylation in normal colon mucosa is associated with poor survival in Chinese patients with sporadic colon cancer

Oncotarget ◽  
2015 ◽  
Vol 6 (27) ◽  
pp. 23820-23836 ◽  
Author(s):  
Changhua Zhuo ◽  
Qingguo Li ◽  
Yuchen Wu ◽  
Yiwei Li ◽  
Jia Nie ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Chinese Patients ◽  
Colon Mucosa ◽  
Poor Survival ◽  
Normal Colon ◽  
Normal Colon Mucosa ◽  
Sporadic Colon Cancer

Cold atmospheric plasma treatment inhibits growth in colorectal cancer cells

2018 ◽  
Vol 400 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Christin Schneider ◽  
Stephanie Arndt ◽  
Julia L. Zimmermann ◽  
Yangfang Li ◽  
Sigrid Karrer ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Plasma Treatment ◽  
Cancer Cells ◽  
Ex Vivo ◽  
Atmospheric Plasma ◽  
Normal Colon ◽  
Colon Tissue ◽  
Cold Atmospheric Plasma ◽  
Colorectal Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

Asymmetric crypt fission in sessile serrated lesions

2020 ◽  
pp. jclinpath-2020-207008 ◽  
Author(s):  
Carlos A Rubio ◽  
Peter T Schmidt
Keyword(s):  
Somatic Mutations ◽  
Colon Mucosa ◽  
Normal Colon ◽  
Normal Colon Mucosa ◽  
Crypt Fission ◽  
Tumour Suppressor Protein ◽  
Serrated Lesions ◽  
Symmetric Fission ◽  
Proliferating Cell ◽  
Asymmetric Fission

ObjectiveSessile serrated lesions without dysplasia (SSL-ND) are epitomised by dilated crypts with epithelial serrations and architectural distortions portraying boot-shapes, L-shapes or inverted-T shapes. Recently, crypts in asymmetric fission were detected in SSL-ND. The purpose was to assess the frequency of crypts in asymmetric fission in a cohort of SSL-ND.MethodsThe frequency of crypts in fission was assessed in 60 SSL-ND, the distribution of cell proliferation in 48 SSL-ND and the expression of maspin, a tumour-suppressor protein, in 29 SSL-ND.ResultsOut of the 60 SSL-ND, 40 (66.7%) showed crypts in fission: 39 (65%) SSL-ND had crypts in asymmetric fission and one SSL-ND (1.7%), in symmetric fission (p<0.05). Of 1495 crypts recorded in the 60 SSL-ND, 73 (4.9%) were in asymmetric fission but only one (0.06%), in symmetric fission (p<0.05). Out of the 48 Ki67-immunostained SSL-ND,15 (31%) showed randomly distributed proliferating cell-domains. All 29 SSL-ND revealed maspin-upregulation (including crypts in asymmetric and symmetric fission). In contrast, the normal colon mucosa showed occasional single crypts in symmetric fission, proliferating cell-domains limited to the lower thirds of the crypts, absence of crypts in asymmetric fission and remained maspin negative.ConclusionsSSL-ND thrive with crypts in asymmetric fission displaying randomly distributed proliferating cell-domains and maspin-upregulation. These histo-biological aberrations disclose pathological cryptogenesis and suggest possibly unfolding somatic mutations in SSL-ND. The present findings may open new vistas on the parameters pertinent to the susceptibility of SSL-ND to develop dysplasia and carcinoma.

scholarly journals Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients

2020 ◽  
Vol 21 (21) ◽  
pp. 8150
Author(s):  
Heike Deutelmoser ◽  
Justo Lorenzo Bermejo ◽  
Axel Benner ◽  
Korbinian Weigl ◽  
Hanla A. Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Prediction Accuracy ◽  
Inverse Association ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Tissue ◽  
Study Population ◽  
The Uk ◽  
Colorectal Cancer Patients

Colorectal cancer (CRC) survival has environmental and inherited components. The expression of specific genes can be inferred based on individual genotypes—so called expression quantitative trait loci. In this study, we used the PrediXcan method to predict gene expression in normal colon tissue using individual genotype data from 91 CRC patients and examined the correlation ρ between predicted and measured gene expression levels. Out of 5434 predicted genes, 58% showed a negative ρ value and only 16% presented a ρ higher than 0.10. We subsequently investigated the association between genotype-based gene expression in colon tissue for genes with ρ > 0.10 and survival of 4436 CRC patients. We identified an inverse association between the predicted expression of ARID3B and CRC-specific survival for patients with a body mass index greater than or equal to 30 kg/m2 (HR (hazard ratio) = 0.66 for an expression higher vs. lower than the median, p = 0.005). This association was validated using genotype and clinical data from the UK Biobank (HR = 0.74, p = 0.04). In addition to the identification of ARID3B expression in normal colon tissue as a candidate prognostic biomarker for obese CRC patients, our study illustrates the challenges of genotype-based prediction of gene expression, and the advantage of reassessing the prediction accuracy in a subset of the study population using measured gene expression data.

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