Leukocyte Alkaline Phosphatase: a Quantitative and Qualitative Study in Normal and Leukemic Cells

1967 ◽  
Vol 8 (6) ◽  
pp. 413-420 ◽  
Author(s):  
A. Tittobello ◽  
A. Agostoni
Keyword(s):  
Alkaline Phosphatase ◽  
Qualitative Study ◽  
Leukemic Cells ◽  
Leukocyte Alkaline Phosphatase

scholarly journals Expression of leukocyte alkaline phosphatase gene in normal and leukemic cells: regulation of the transcript by granulocyte colony- stimulating factor

Blood ◽  
1990 ◽  
Vol 76 (12) ◽  
pp. 2565-2571 ◽  
Author(s):  
A Rambaldi ◽  
M Terao ◽  
S Bettoni ◽  
ML Tini ◽  
R Bassan ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Lymphoblastic Leukemia ◽  
Lymphocytic Leukemia ◽  
Myelogenous Leukemia ◽  
Leukemic Cells ◽  
Polymorphonuclear Cells ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Leukocyte Alkaline Phosphatase ◽  
Stimulating Factor

Abstract The levels of leukocyte alkaline phosphatase (LAP) messenger RNA (mRNA) are evaluated in B and T lymphocytes, monocytes, and polymorphonuclear cells (PMNs), and this transcript is found to be present only in PMNs. Precursors of the myelomonocytic pathway, represented by leukemic cells isolated from several cases of chronic myelogenous leukemia (CML) in its stable and blastic phase and acute myelogenous leukemia (AML), are devoid of LAP transcript. These data support the notion that LAP is a marker of the granulocyte terminal differentiation. Despite the absence of LAP mRNA in both the myeloid and the lymphoid precursors, nuclear run-on experiments show constitutive transcription of the LAP gene in leukemic cells obtained from AML, CML, as well as acute lymphoblastic leukemia (ALL) and B-cell chronic lymphocytic leukemia (B-CLL). In CML and in chronic myelo-monocytic leukemia (CMML) PMNs, granulocyte colony- stimulating factor (G-CSF) specifically accumulates LAP mRNA without showing a substantial increase in the rate of transcription of the LAP gene. Once increased by G-CSF, LAP mRNA is very stable, showing a half- life of more than 4 hours in the presence of actinomycin-D. G-CSF is suggested to play a pivotal role in the modulation of LAP transcript in PMNs.

scholarly journals Ultrastructural cytochemical analysis of blastic transformation of chronic myelocytic leukemia

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 535-547
Author(s):  
P Palakavongs ◽  
S Teichberg ◽  
V Vinciguerra ◽  
TJ Degnan ◽  
P Sinlaratana
Keyword(s):  
Alkaline Phosphatase ◽  
Nuclear Envelope ◽  
Secretory Granules ◽  
Leukemic Cells ◽  
Blast Transformation ◽  
Chronic Myelocytic Leukemia ◽  
Blast Phase ◽  
Blastic Transformation ◽  
Myelocytic Leukemia ◽  
Leukocyte Alkaline Phosphatase

Ultrastructural cytochemical changes occurring during the blast phase of chronic myelocytic leukemia (CML) are described. Normal developing promyelocytes contain myeloperoxidase (MPO) -positive rough endoplasmic reticulum, nuclear envelope, and Golgi apparatus. All secretory granules of normal promyelocytes are also MPO-positive. In this study we have found abnormal promyelocytes with MPO-positive as well as MPO- negative secretory granules in blast phase CML patients which contrast with the normal pattern of MPO distribution in most CML patients not in the blast phase or in nonleukemic controls. Alkaline phosphatase activity was found in the nuclear envelope of blasts and promyelocytes of one of the blast transformation patients who had a markedly increased leukocyte alkaline phosphatase score. The cytochemical changes in the distribution of MPO suggest that immature leukemic cells may alter their patterns of secretory granule production. Such processes may reflect the emergence of an abnormal clone of cells during the blastic transformation of CML.

scholarly journals Expression of leukocyte alkaline phosphatase gene in normal and leukemic cells: regulation of the transcript by granulocyte colony- stimulating factor

Blood ◽  
1990 ◽  
Vol 76 (12) ◽  
pp. 2565-2571 ◽  
Author(s):  
A Rambaldi ◽  
M Terao ◽  
S Bettoni ◽  
ML Tini ◽  
R Bassan ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Lymphoblastic Leukemia ◽  
Lymphocytic Leukemia ◽  
Myelogenous Leukemia ◽  
Leukemic Cells ◽  
Polymorphonuclear Cells ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Leukocyte Alkaline Phosphatase ◽  
Stimulating Factor

The levels of leukocyte alkaline phosphatase (LAP) messenger RNA (mRNA) are evaluated in B and T lymphocytes, monocytes, and polymorphonuclear cells (PMNs), and this transcript is found to be present only in PMNs. Precursors of the myelomonocytic pathway, represented by leukemic cells isolated from several cases of chronic myelogenous leukemia (CML) in its stable and blastic phase and acute myelogenous leukemia (AML), are devoid of LAP transcript. These data support the notion that LAP is a marker of the granulocyte terminal differentiation. Despite the absence of LAP mRNA in both the myeloid and the lymphoid precursors, nuclear run-on experiments show constitutive transcription of the LAP gene in leukemic cells obtained from AML, CML, as well as acute lymphoblastic leukemia (ALL) and B-cell chronic lymphocytic leukemia (B-CLL). In CML and in chronic myelo-monocytic leukemia (CMML) PMNs, granulocyte colony- stimulating factor (G-CSF) specifically accumulates LAP mRNA without showing a substantial increase in the rate of transcription of the LAP gene. Once increased by G-CSF, LAP mRNA is very stable, showing a half- life of more than 4 hours in the presence of actinomycin-D. G-CSF is suggested to play a pivotal role in the modulation of LAP transcript in PMNs.

scholarly journals Ultrastructural cytochemical analysis of blastic transformation of chronic myelocytic leukemia

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 535-547 ◽  
Author(s):  
P Palakavongs ◽  
S Teichberg ◽  
V Vinciguerra ◽  
TJ Degnan ◽  
P Sinlaratana
Keyword(s):  
Alkaline Phosphatase ◽  
Nuclear Envelope ◽  
Secretory Granules ◽  
Leukemic Cells ◽  
Blast Transformation ◽  
Chronic Myelocytic Leukemia ◽  
Blast Phase ◽  
Blastic Transformation ◽  
Myelocytic Leukemia ◽  
Leukocyte Alkaline Phosphatase

Abstract Ultrastructural cytochemical changes occurring during the blast phase of chronic myelocytic leukemia (CML) are described. Normal developing promyelocytes contain myeloperoxidase (MPO) -positive rough endoplasmic reticulum, nuclear envelope, and Golgi apparatus. All secretory granules of normal promyelocytes are also MPO-positive. In this study we have found abnormal promyelocytes with MPO-positive as well as MPO- negative secretory granules in blast phase CML patients which contrast with the normal pattern of MPO distribution in most CML patients not in the blast phase or in nonleukemic controls. Alkaline phosphatase activity was found in the nuclear envelope of blasts and promyelocytes of one of the blast transformation patients who had a markedly increased leukocyte alkaline phosphatase score. The cytochemical changes in the distribution of MPO suggest that immature leukemic cells may alter their patterns of secretory granule production. Such processes may reflect the emergence of an abnormal clone of cells during the blastic transformation of CML.

scholarly journals About Leukocyte Alkaline Phosphatase in AIDS

1990 ◽  
Vol 83 (4) ◽  
pp. 214-214
Author(s):  
J. Grozdea ◽  
A. Brisson-Lougarre ◽  
H. Vergnes ◽  
S. Alie-Daram ◽  
J. Martin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase

Leukocyte alkaline phosphatase in pregnancy and the puerperium

1970 ◽  
Vol 107 (4) ◽  
pp. 604-609 ◽  
Author(s):  
W.Z. Polishuk ◽  
Y.Z. Diamant ◽  
H. Zuckerman ◽  
E. Sadovsky
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase ◽  
In Pregnancy

scholarly journals Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay

2007 ◽  
Vol 21 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Marília P.P. Kanegae ◽  
Valdecir F. Ximenes ◽  
Roberto P. Falcão ◽  
Virgílio A.R. Colturato ◽  
Éderson R. de Mattos ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase
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