Value of Leukocyte Alkaline Phosphatase and Other Leukocyte Parameters in Diagnosis of Neonatal Infection

Neonatology ◽  
1984 ◽  
Vol 45 (6) ◽  
pp. 275-279 ◽  
Author(s):  
Ravinderjeet S. Paul ◽  
Ashir Kumar
Keyword(s):  
Alkaline Phosphatase ◽  
Neonatal Infection ◽  
Leukocyte Alkaline Phosphatase

scholarly journals About Leukocyte Alkaline Phosphatase in AIDS

1990 ◽  
Vol 83 (4) ◽  
pp. 214-214
Author(s):  
J. Grozdea ◽  
A. Brisson-Lougarre ◽  
H. Vergnes ◽  
S. Alie-Daram ◽  
J. Martin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase

Leukocyte alkaline phosphatase in pregnancy and the puerperium

1970 ◽  
Vol 107 (4) ◽  
pp. 604-609 ◽  
Author(s):  
W.Z. Polishuk ◽  
Y.Z. Diamant ◽  
H. Zuckerman ◽  
E. Sadovsky
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase ◽  
In Pregnancy

scholarly journals Chemiluminescent determination of leukocyte alkaline phosphatase: an advantageous alternative to the cytochemical assay

2007 ◽  
Vol 21 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Marília P.P. Kanegae ◽  
Valdecir F. Ximenes ◽  
Roberto P. Falcão ◽  
Virgílio A.R. Colturato ◽  
Éderson R. de Mattos ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Leukocyte Alkaline Phosphatase

RAPID, SENSITIVE N0N-RADI0ACTIVE QUANTIFICATION AND ANALYSIS OF PLASMA VON WILLEBRAND FACTOR (vWF) MULTIMERS

1987 ◽  
Author(s):  
J L Moake ◽  
M A Harris ◽  
C E Whitley ◽  
C P Alfrey
Keyword(s):  
Alkaline Phosphatase ◽  
Milk Proteins ◽  
Classical Type ◽  
Type I ◽  
Leukocyte Alkaline Phosphatase ◽  
Red Color ◽  
Alternative Techniques ◽  
Relative Deficiency ◽  
Von Willebrand ◽  
Willebrand Factor

Assessment of plasma vWF abnormalities by clinical coagulation laboratories is difficult because the available test systems for vWF antigen quantification and multimer analysis are expensive, laborious, and require days, radioactive anti-vWF antibodies and autoradiographic methods. We have devised simple, rapid, sensitive alternative techniques for vWF quantification and multimer analysis that can be readily installed in clinical laboratories. Plasma vWF antigen quantification is by a 2 hour enzyme immunoassay that accurately detects levels as low as 0.23% of normal. Plasma vWF to be quantified is bound to polyclonal monospecific antihuman vWF attached to small glass beads, and anti-human vWF conjugated with alkaline phosphatase is added to make an insoluble "sandwich." A substrate solution consisting of phenylphosphate and 4-amino-antipurine is added, followed by potassium ferricyanide. Optical density (at 490-510 nm) of the red color that develops is directly proportional to the plasma concentration of vWF antigen. Plasma vWF multimeric analysis is by a one-day electrophoretic immunobiot procedure. Plasma vWF multimer forms are solubilized in SDS-urea-Tris-EDTA, separated by horizontal 1% agarose gel electrophoresis, and transferred to a cationic membrane. Other protein binding sites on the membrane are blocked with milk proteins, and the membrane is overlaid with anti-vWF IgG linked to alkaline phosphatase. vWF multimers are then displayed as blue bands by soaking the membrane in an alkaline solution of the histochemical stain, fast blue RR (commonly used for leukocyte alkaline phosphatase scoring) dissolved in naphtol AS-MX phosphate. These simple, non-radioactive procedures performed together permit the rapid distinction of classical (Type I) von Willebrand's disease (vWD), characterized by low vWF antigen and normal multimers, from the Type II vWD syndromes, characterized by a relative deficiency of the largest plasma vWF forms. Unusually large vWF multimers, present in remission plasma of patients with chronic relapsing thrombotic thrombocytopenic purpura (TTP), are also easily detected using this rapid system of multimer analysis.

scholarly journals Endocrine Relationships of Leukocyte Alkaline Phosphatase

Blood ◽  
1965 ◽  
Vol 25 (3) ◽  
pp. 356-369 ◽  
Author(s):  
FRED ROSNER ◽  
STANLEY L. LEE
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Mean Value ◽  
Conclusive Evidence ◽  
In Vitro Tests ◽  
Enzyme Release ◽  
Men And Women ◽  
Leukocyte Alkaline Phosphatase

Abstract Leukocyte alkaline phosphatase activity has been noted to be different in men and women. The mean leukocyte alkaline phosphatase activity for 74 normal men, aged 19 to 60 years, was 23 mg. of phosphorus per 1010 polvmorphonuclear leukocytes per hour. The corresponding mean value for 75 normal young women, age 19-48 years, was 35 (p < .001). No significant differences between boys and girls occurred until the time of puberty. After the menopause, the values for women approached the values for men. Women treated with androgens had lower leukocyte alkaline phosphatase activity than did control women. These results suggest that androgenic hormones inhibit this enzyme, and that other, as yet undefined endocrine influences, also affect its level of activity. In vitro tests with various concentrations of androgens and estrogens failed to provide conclusive evidence of direct effect on leukocytes although some degree of direct inhibition by androgens was suggested. Studies using saponin to effect enzyme release from leukocyte granules did not demonstrate whether the differences between men and women are differences of enzyme release or of content of leukocyte alkaline phosphatase.

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