Frequency of Application of AmF/NaF/SnCl2 Solution and Its Potential in Controlling Human Enamel Erosion Progression: An in situ Study

2017 ◽  
Vol 51 (2) ◽  
pp. 141-148 ◽  
Author(s):  
C.V. da Silva ◽  
T.M. Ramos-Oliveira ◽  
T.F. Mantilla ◽  
P.M. de Freitas
Keyword(s):  
Citric Acid ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Negative Control ◽  
Tissue Loss ◽  
In Situ Study ◽  
Acid Ph ◽  
Human Enamel

Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 × 2 min/day) and rinsing protocol (1 or 2 × 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (±SD in µm) for each treatment protocol and statistical differences were found as follows: G1 4.55 ± 2.75, G2 4.59 ± 2.13, G3 2.64 ± 1.55, and G4 1.34 ± 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.

Optimizing citric acid protocol to control implant‐related infections: An in vitro and in situ study

2021 ◽  
Author(s):  
Jairo M. Cordeiro ◽  
Júlia M. Pires ◽  
João G. S. Souza ◽  
Carolina V. Lima ◽  
Martinna M. Bertolini ◽  
...  
Keyword(s):  
Citric Acid ◽  
In Situ Study

The Erosive Potential of Different Malt Drinks: An in vitro and in situ study

2008 ◽  
Vol 33 (1) ◽  
pp. 35-37 ◽  
Author(s):  
Esber Çaglar ◽  
Sule Kavaloglu Cildir ◽  
Nuket Sandalli
Keyword(s):  
Potential Effect ◽  
Dental Erosion ◽  
Variable Degree ◽  
Water Control ◽  
In Situ Study ◽  
Significant Difference ◽  
Natural Spring ◽  
Erosive Effect

Objectives: Whereas the potential effect of acidic drinks in the etiology of dental erosion is well recognized the role of malt drinks is unclear. The primary aim of the present study was to compare the in vitro erosive effect on enamel produced by different aromated malt drinks. A secondary objective was to compare their erosive effects in situ with those determined in vitro. Materials and methods: To select the malt drink for the study in situ, six commercially available malt drinks were examined for erosive potential in vitro. The study in situ was a single centre, 2-period, 2-treatment crossover study to compare the erosive effect of a commercially available malt drink (Test), with that of natural spring water (Control), over 10 day periods on 10 healthy volunteers. Subjects wore upper removable appliances containing two human enamel specimens from 9 a.m. to 4 p.m. The regimen of intake of the drinks was 250 ml at midday. Measurements of enamel loss were made on samples after 5 and 10 days by profilometry. Results: The in situ study showed a statistically significant difference in erosive potential between the test and control beverages. No specimen exposed to the control beverage displayed appreciable erosion. Erosion occurred with the test drink, but to a variable degree between subjects. Conclusions: Malt drinks should be considered as potentially erosive as the results for enamel specimens exposed to the test beverage in the clinical study showed a degree of erosion that varied greatly between different participants. It is likely that under these conditions an increase in the degree of erosion would be observed in children and young people who consume malt drinks.

Hard-tissue Substrates for Evaluation of Cariogenic and Anti-cariogenic Activity in situ

1992 ◽  
Vol 71 (3_suppl) ◽  
pp. 913-919 ◽  
Author(s):  
J.R. Mellberg
Keyword(s):  
Root Surface ◽  
Hard Tissue ◽  
Suitable Alternative ◽  
Human Enamel ◽  
Human Dentin ◽  
Bovine Enamel ◽  
Root Surface Caries

Hard-tissue substrates include primarily human and bovine enamel and human dentin. They have been used for in situ studies in a natural or sound condition, as well as flattened or containing an in vitro-formed caries-like lesion. Human enamel and dentin are generally the substrates of choice for studies of coronal and root-surface caries, respectively, but bovine enamel appears to offer a suitable alternative for many studies of enamel caries. Substrates with caries-like lesions will respond more rapidly to changes in the intra-oral mineral equilibrium and will allow both demineralization and remineralization to be determined. Findings from some studies suggest that caries-like lesions may respond somewhat differently, depending upon the degree of mineralization of the surface layer. Because in vitro findings with dentin show it to be significantly more soluble in acid than enamel, results from a study that used dentin may not be directly applicable to enamel. Both enamel and dentin substrates can be used in thin-section models. Hard-tissue substrates can also differ, depending upon their intra-oral location. Locations that result in the accumulation of plaque will behave differently from those that are plaque-free. So that plaque would accumulate, substrates have been placed approximally, beneath a fabric or steel mesh, in a protected trough, beneath a metal band or within a depression on the buccal surface. For studies requiring a determination of both demineralization and remineralization, human enamel or dentin containing a surface-softened caries-like lesion and covered with a uniform natural plaque are the substrates of choice.

scholarly journals Fluoride Release from Fluoride Varnishes under Acidic Conditions

2014 ◽  
Vol 39 (1) ◽  
pp. 35-39 ◽  
Author(s):  
F Lippert
Keyword(s):  
Citric Acid ◽  
Artificial Saliva ◽  
Fluoride Varnish ◽  
Laboratory Research ◽  
Fluoride Release ◽  
Acidic Solutions ◽  
Acid Ph ◽  
Opposite Behavior ◽  
Acidic Conditions

Objective: The aim was to investigate the in vitro fluoride release from fluoride varnishes under acidic conditions. Study design: Poly(methyl methacrylate) blocks (Perspex, n=3 per group) were painted with 80±5 mg fluoride varnish (n=10) and placed into artificial saliva for 30min. Then, blocks were placed into either 1% citric acid (pH 2.27) or 0.3% citric acid (pH 3.75) solutions (n=3 per solution and varnish) for 30min with the solutions being replaced every 5min. Saliva and acid solutions were analyzed for fluoride content. Data were analyzed using three-way ANOVA (varnish, solution, time). Results: The three-way interaction was significant (p>0.0001). Fluoride release and release patterns varied considerably between varnishes. Fluoride release in saliva varied by a factor of more than 10 between varnishes. Some varnishes (CavityShield, Nupro, ProFluorid, Vanish) showed higher fluoride release in saliva than during the first 5min of acid exposure, whereas other varnishes (Acclean, Enamel-Pro, MI Varnish, Vella) showed the opposite behavior. There was little difference between acidic solutions. Conclusions: Fluoride release from fluoride varnishes varies considerably and also depends on the dissolution medium. Bearing in mind the limitations of laboratory research, the consumption of acidic drinks after fluoride varnish application should be avoided to optimize the benefit/risk ratio.

scholarly journals Effect of Hydrogen and Carbamide Peroxide in Bleaching, Enamel Morphology, and Mineral Composition: In vitro Study

2017 ◽  
Vol 18 (7) ◽  
pp. 576-582 ◽  
Author(s):  
Carmen Llena ◽  
Leopoldo Forner ◽  
Irene Esteve
Keyword(s):  
Mineral Composition ◽  
Laser Scanning ◽  
Morphological Changes ◽  
Treatment Protocol ◽  
Environmental Scanning Electron Microscopy ◽  
In Vitro Study ◽  
Confocal Laser ◽  
Carbamide Peroxide ◽  
Tooth Color

ABSTRACT Aim The aim of the study was to evaluate the bleaching effect, morphological changes, and variations in calcium (Ca) and phosphate (P) in the enamel with hydrogen peroxide (HP) and carbamide peroxide (CP) after the use of different application regimens. Materials and methods Four groups of five teeth were randomly assigned, according to the treatment protocol: HP 37.5% applied for 30 or 60 minutes (HP30, HP60), CP 16% applied for 14 or 28 hours (CP14, CP28). Changes in dental color were evaluated, according to the following formula: ΔE = [(La−Lb)2+(aa−ab)2 + (ba−bb)2]½. Enamel morphology and Ca and P compositions were evaluated by confocal laser scanning microscope and environmental scanning electron microscopy. Results ΔE HP30 was significantly greater than CP14 (10.37 ± 2.65/8.56 ± 1.40), but not between HP60 and CP28. HP60 shows greater morphological changes than HP30. No morphological changes were observed in the groups treated with CP. The reduction in Ca and P was significantly greater in HP60 than in CP28 (p < 0.05). Conclusion Both formulations improved tooth color; HP produced morphological changes and Ca and P a gradual decrease, while CP produced no morphological changes, and the decrease in mineral component was smaller. Clinical significance CP 16% applied during 2 weeks could be equally effective and safer for tooth whitening than to administer two treatment sessions with HP 37.5%. How to cite this article Llena C, Esteve I, Forner L. Effect of Hydrogen and Carbamide Peroxide in Bleaching, Enamel Morphology, and Mineral Composition: In vitro Study. J Contemp Dent Pract 2017;18(7):576-582.

scholarly journals Protective effect of green tea catechins on eroded human dentin: an in vitro/in situ study

2021 ◽  
Vol 35 ◽  
Author(s):  
Maria Denise Rodrigues DE MORAES ◽  
Vanara Florêncio PASSOS ◽  
Gislaine Cristina PADOVANI ◽  
Lady Clarissa Brito da Rocha BEZERRA ◽  
Ilka Maria VASCONCELOS ◽  
...  
Keyword(s):  
Protective Effect ◽  
Green Tea ◽  
Tea Catechins ◽  
In Situ Study ◽  
Green Tea Catechins ◽  
Human Dentin

scholarly journals Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study

2012 ◽  
Vol 13 (3) ◽  
pp. 405-415 ◽  
Author(s):  
LM Ranganath ◽  
B Sunil Rao ◽  
AG Rajesh ◽  
KS Prem Kumar
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Morphological Changes ◽  
Carbamide Peroxide ◽  
Electron Microscopic ◽  
Human Enamel ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

ABSTRACT Objective The purpose of this study was to evaluate, using scanning electron microscopy (SEM), the morphological and structural changes of the enamel induced by three bleaching agents namely old McInnes solution, modified McInnes solution and 10% carbamide peroxide gel at different time intervals. Materials and methods Fifteen freshly extracted noncarious human central incisors with intact enamel surface were selected. The teeth were sectioned at the cementoenamel junction separating the crown portion from the root using a diamond separating disk. Following this, the samples were subjected to three different bleaching agents: Group 1: Old McInnes solution, group 2: modified McInnes solution and group 3: 10% carbamide peroxide for a period of 15, 30 and 60 minutes, 24 and 30 hours time interval. The sample stubs were subjected to scanning electron microscope and were photographed at 2000 and 10,000 magnifications. Conclusion The present study revealed no indication of either etching or significant change in surface morphology of enamel when evaluated under scanning electron microscope after 6 weeks treatment with various bleaching agents. Clinical significance Morphological alterations in bleached enamel are both concentration and time dependent. How to cite this article Rajesh AG, Ranganath LM, Kumar KSP, Rao BS. Surface Morphological Changes in Human Enamel Following Bleaching: An in vitro Scanning Electron Microscopic Study. J Contemp Dent Pract 2012;13(3):405-415.

scholarly journals MiR-29b may suppresses peritoneal metastases through inhibition of the mesothelial–mesenchymal transition (MMT) of human peritoneal mesothelial cells

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Yuki Kimura ◽  
Hideyuki Ohzawa ◽  
Hideyo Miyato ◽  
Yuki Kaneko ◽  
Akira Saito ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Negative Control ◽  
Mesothelial Cells ◽  
Peritoneal Metastases ◽  
Dependent Manner ◽  
Mesenchymal Transition ◽  
Peritoneal Mesothelial Cells ◽  
Human Peritoneal Mesothelial Cells ◽  
Tgf Β1

AbstractPeritoneal dissemination is a major metastatic pathway for gastrointestinal and ovarian malignancies. The miR-29b family is downregulated in peritoneal fluids in patients with peritoneal metastases (PM). We examined the effect of miR-29b on mesothelial cells (MC) which play critical a role in the development of PM through mesothelial-mesenchymal transition (MMT). Human peritoneal mesothelial cells (HPMCs) were isolated from surgically resected omental tissue and MMT induced by stimulation with 10 ng/ml TGF-β1. MiR-29b mimics and negative control miR were transfected by lipofection using RNAiMAX and the effects on the MMT evaluated in vitro. HPMC produced substantial amounts of miR-29b which was markedly inhibited by TGF-β1. TGF-β1 stimulation of HPMC induced morphological changes with decreased expression of E-cadherin and calretinin, and increased expression of vimentin and fibronectin. TGF-β1 also enhanced proliferation and migration of HPMC as well as adhesion of tumor cells in a fibronectin dependent manner. However, all events were strongly abrogated by simultaneous transfection of miR-29b. MiR-29b inhibits TGF-β1 induced MMT and replacement of miR-29b in the peritoneal cavity might be effective to prevent development of PM partly through the effects on MC.

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