Remineralizing Potential of a Low Fluoride Toothpaste with Sodium Trimetaphosphate: An in situ Study

2016 ◽  
Vol 50 (6) ◽  
pp. 571-578 ◽  
Author(s):  
Eliana M. Takeshita ◽  
Marcelle Danelon ◽  
Luciene P. Castro ◽  
Robson F. Cunha ◽  
Alberto C. B. Delbem
Keyword(s):  
Oral Cavity ◽  
Repeated Measures ◽  
Surface Hardness ◽  
Repeated Measures Anova ◽  
Treatment Groups ◽  
Fluoride Toothpaste ◽  
Sodium Trimetaphosphate ◽  
In Situ Study ◽  
Bovine Enamel

Objective: To evaluate the effect of a low-fluoride (F) toothpaste supplemented with sodium trimetaphosphate (TMP) on enamel remineralization in situ. Design: Bovine enamel blocks were selected on the basis of their surface hardness (SH) after caries-like lesions had been induced, and randomly divided into 4 treatment groups, according to the toothpastes used: without F or TMP (placebo); 500 ppm F; 500 ppm F plus 1% TMP; and 1,100 ppm F. The study design was blinded and crossover and performed in 4 phases of 3 days each. Eleven subjects used palatal appliances containing 4 bovine enamel blocks which were treated 3 times per day during 1 min each time, with natural slurries of saliva and toothpaste formed in the oral cavity during toothbrushing. After each phase, the percentages of surface (%SHR) and subsurface hardness recovery (%ΔKHNR) were calculated. F, calcium (Ca), and phosphorus (Pi) contents in enamel were also determined. Data were analyzed by 1-way, repeated-measures ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). Results: Toothpaste with 500 ppm F + TMP and 1,100 ppm F showed similar %SHR and %ΔKHNR as well as enamel F, Ca, and Pi concentrations. Conclusion: The addition of TMP to a low-fluoride toothpaste promoted a similar remineralizing capacity to that of a standard (1,100 ppm F) toothpaste in situ.

Effect of Fluoride Toothpaste Containing Nano-Sized Sodium Hexametaphosphate on Enamel Remineralization: An in situ Study

2018 ◽  
Vol 53 (3) ◽  
pp. 260-267 ◽  
Author(s):  
Marcelle Danelon ◽  
Luhana G. Garcia ◽  
Juliano P. Pessan ◽  
Amanda Passarinho ◽  
Emerson R. Camargo ◽  
...  
Keyword(s):  
Surface Hardness ◽  
Sodium Hexametaphosphate ◽  
Treatment Groups ◽  
Fluoride Toothpaste ◽  
Integrated Recovery ◽  
Phase Surface ◽  
Double Blinded ◽  
Bovine Enamel ◽  
Caries Lesions

Objective: To evaluate the remineralizing potential of a conventional toothpaste (1,100 ppm F) supplemented with nano-sized sodium hexametaphosphate (HMPnano) in artificial caries lesions in situ. Design: This double-blinded crossed study was performed in 4 phases of 3 days each. Twelve subjects used palatal appliances containing 4 bovine enamel blocks with artificial caries lesions. Volunteers were randomly assigned into the following treatment groups: no F/HMP/HMPnano (Placebo); 1,100 ppm F (1100F); 1100F plus 0.5% micrometric HMP (1100F/HMP) and 1100F plus 0.5% nano-sized HMP (1100F/HMPnano). Volunteers were instructed to brush their natural teeth with the palatal appliances in the mouth for 1 min (3 times/day), so that blocks were treated with natural slurries of toothpastes. After each phase, surface hardness post-remineralization (SH2), integrated recovery of subsurface hardness (ΔIHR), integrated mineral recovery (ΔIMR) and enamel F concentration were determined. Data were submitted to analysis of variance and Student-Newman-Keuls’ test (p < 0.001). Results: Enamel surface became 42% harder when treated with 1100F/HMPnano in comparison with 1100F (p < 0.001). Treatment with 1100F/HMP and 1100F/HMPnano promoted an increase of ∼23 and ∼87%, respectively, in ΔIHR when compared to 1100F (p < 0.001). In addition, ΔIMR for the 1100F/HMPnano was ∼75 and ∼33% higher when compared to 1100F and 1100F/HMP respectively (p < 0.001). Enamel F uptake was similar among all groups except for the placebo (p < 0.001). Conclusion: The addition of 0.5% HMPnano to a conventional fluoride toothpaste was able to promote an additional remineralizing effect of artificial caries lesions.

Toothpaste with Nanosized Trimetaphosphate Reduces Enamel Demineralization

2016 ◽  
Vol 2 (3) ◽  
pp. 233-240 ◽  
Author(s):  
M.D.B. Souza ◽  
J.P. Pessan ◽  
C.S. Lodi ◽  
J.A.S. Souza ◽  
E.R. Camargo ◽  
...  
Keyword(s):  
Protective Effect ◽  
Surface Hardness ◽  
Extracellular Polysaccharide ◽  
Degree Of Saturation ◽  
Double Blind ◽  
Cross Sectional ◽  
Fluoride Toothpaste ◽  
Sodium Trimetaphosphate ◽  
Enamel Demineralization

This double-blind crossover study assessed the effects of a low-fluoride (low-F) dentifrice containing nanosized sodium trimetaphosphate (TMP) on enamel demineralization in situ. Nineteen subjects wore palatal appliances containing 4 blocks of bovine enamel and were randomly assigned to brush their teeth with placebo (without F/TMP), 250-ppm F (250F), 250F plus 0.05% nanosized TMP (250F-TMPnano), and 1,100-ppm F (1,100F) dentifrices during 7 d, under cariogenic challenge. Enamel surface hardness and cross-sectional hardness (ΔKHN [Knoop hardness number]), as well as F, calcium (Ca), and phosphorus (P) concentrations, were determined. Also, biofilm that formed on the blocks was analyzed for F, Ca, P, and insoluble extracellular polysaccharide concentrations. Data were submitted to analysis-of-variance models and Student-Newman-Keuls test ( P < 0.05). The 250F-TMPnano dentifrice promoted the lowest ΔKHN among all groups ( P < 0.001), while the percentage of surface hardness loss was similar to 1,100F. Also, similar F, Ca, and P concentrations in enamel were observed for 1,100F and 250F-TMPnano. In the biofilm, the highest F content was observed for 1,100F; Ca content was similar between 1,100F and 250F-TMPnano; and P content was similar among all groups. Similar extracellular polysaccharide values were observed for 250F-TMPnano and 1,100F ( P < 0.001), ionic activity of CaHPO40, CaF+, and HF0 ( P < 0.05) and degree of saturation of hydroxyapatite and CaF2 ( P < 0.05). It was concluded that the protective effect of 250F-TMPnano dentifrice was similar to a conventional dentifrice for most of the variables studied, having a more pronounced effect on the subsurface lesion when compared with the conventional toothpaste (1,100F). Knowledge Transfer Statement: Although toothpastes containing ≥1,000-ppm fluoride are more effective than low-fluoride formulations against dental caries, their early use can lead to side effects. This has prompted intensive research on alternatives to increase the anticaries effect of low-fluoride toothpastes. The present in situ study demonstrated that the addition of sodium trimetaphosphate nanoparticles to toothpastes containing 250-ppm fluoride significantly enhances the protective effect of this formulation against enamel demineralization to levels comparable to a 1,100-ppm fluoride toothpaste in terms of most of the variables studied. Most important, this formulation promoted the lowest loss of subsurface hardness among all groups, suggesting that caries lesions would take longer to develop under clinical conditions when compared with a conventional (1,100-fluoride) toothpaste.

scholarly journals In Vitro Enamel Remineralization by Low-Fluoride Toothpaste with Calcium Citrate and Sodium Trimetaphosphate

2013 ◽  
Vol 24 (3) ◽  
pp. 253-257 ◽  
Author(s):  
Edo Hirata ◽  
Marcelle Danelon ◽  
Isabelle Rodrigues Freire ◽  
Alberto Carlos Botazzo Delbem
Keyword(s):  
Artificial Saliva ◽  
Fluoride Concentration ◽  
Surface Hardness ◽  
Positive Control ◽  
Calcium Citrate ◽  
Fluoride Toothpaste ◽  
Sodium Trimetaphosphate ◽  
Ph Cycling ◽  
Bovine Enamel

The objective of this study was to evaluate in vitro the effect of a low fluoride toothpaste (450 µgF/g, NaF) combined with calcium citrate (Cacit) and sodium trimetaphosphate (TMP) on enamel remineralization. Bovine enamel blocks had the enamel surface polished sequentially to determine the surface hardness. After production of artificial carious lesions, the blocks selected by their surface hardness were submitted to remineralization pH cycling and daily treatment with dentifrice suspensions (diluted in deionized water or artificial saliva): placebo, 275, 450, 550 and 1,100 µgF/g and commercial dentifrice (positive control, 1,100 µgF/g). Finally, the surface and cross-section hardness was determined for calculating the change of surface hardness (%SH) and mineral content (%∆Z). Fluoride in enamel was also determined. The data from %SH, %∆Z and fluoride were subjected to two-way analysis of variance followed by Student-Newman-Keuls's test (p<0.05). The mineral gain (%SH and %∆Z) was higher for toothpastes diluted in saliva (p<0.05), except for the 450 µgF/g dentifrice with Cacit/TMP (p>0.05). The 450 Cacit/TMP toothpaste and the positive control showed similar results (p>0.05) when diluted in water. A dose-response was observed between fluoride concentration in toothpastes and fluoride present in enamel, regardless of dilution. It was concluded that it is possible to enhance the remineralization capacity of low F concentration toothpaste by of organic (Cacit) and inorganic (TMP) compounds with affinity to hydroxyapatite.

scholarly journals Effect of Hydrogen Peroxide at 35% on the Morphology of Enamel and Interference in the De-remineralization Process: An In Situ Study

2012 ◽  
Vol 37 (5) ◽  
pp. 518-525 ◽  
Author(s):  
AM de Arruda ◽  
PH dos Santos ◽  
RH Sundfeld ◽  
SB Berger ◽  
ALF Briso
Keyword(s):  
Hydrogen Peroxide ◽  
Dental Caries ◽  
Microscopic Analysis ◽  
Enamel Surface ◽  
In Situ Study ◽  
Bovine Enamel ◽  
Histologic Changes

SUMMARY This study evaluated the microhardness and histomorphology of bovine enamel when 35% hydrogen peroxide is used. A total of 44 specimens were adapted to removable devices used by 11 individuals subjected to dental caries challenge. A decrease in microhardness was observed for all groups after the cariogenic challenge. Microscopic analysis revealed that fragments subjected to cariogenic challenge associated with bleaching had more intense superficial histologic changes, but the depth of the lesions remained unchanged. It was concluded that 35% hydrogen peroxide enhanced the reduction in hardness and histomorphologic changes in the enamel surface exposed to cariogenic challenge.

Re- and Demineralization Characteristics of Enamel Depending on Baseline Mineral Loss and Lesion Depth in situ

2016 ◽  
Vol 50 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Richard J. Wierichs ◽  
Julian Lausch ◽  
Hendrik Meyer-Lueckel ◽  
Marcella Esteves-Oliveira
Keyword(s):  
Negative Control ◽  
Pronounced Change ◽  
Protocol Violation ◽  
Lesion Depth ◽  
Treatment Groups ◽  
Before And After ◽  
Double Blinded ◽  
Bovine Enamel ◽  
Mineral Loss

Objectives: The aim of this double-blinded, randomized, cross-over in situ study was to evaluate the re- and demineralization characteristics of sound enamel as well as lowly and highly demineralized caries-like enamel lesions after the application of different fluoride compounds. Methods: In each of three experimental legs of 4 weeks, 21 participants wore intraoral mandibular appliances containing 4 bovine enamel specimens (2 lowly and 2 highly demineralized). Each specimen included one sound enamel and either one lowly demineralized (7 days, pH 4.95) or one highly demineralized (21 days, pH 4.95) lesion, and was positioned 1 mm below the acrylic under a plastic mesh. The three randomly allocated treatments (application only) included the following dentifrices: (1) 1,100 ppm F as NaF, (2) 1,100 ppm F as SnF2 and (3) 0 ppm F (fluoride-free) as negative control. Differences in integrated mineral loss (ΔΔZ) and lesion depth (ΔLD) were calculated between values before and after the in situ period using transversal microradiography. Results: Of the 21 participants, 6 did not complete the study and 2 were excluded due to protocol violation. Irrespectively of the treatment, higher baseline mineral loss and lesion depth led to a less pronounced change in mineral loss and lesion depth. Except for ΔΔZ of the dentifrice with 0 ppm F, sound surfaces showed significantly higher ΔΔZ and ΔLD values compared with lowly and highly demineralized lesions (p < 0.05, t test). Conclusion: Re- and demineralization characteristics of enamel depended directly on baseline mineral loss and lesion depth. Treatment groups should therefore be well balanced with respect to baseline mineral loss and lesion depth.

In situ effect of fluoride toothpaste supplemented with nano-sized sodium trimetaphosphate on enamel demineralization prevention and biofilm composition

2018 ◽  
Vol 96 ◽  
pp. 223-229 ◽  
Author(s):  
Nayara Gonçalves Emerenciano ◽  
Alberto Carlos Botazzo Delbem ◽  
Juliano Pelim Pessan ◽  
Gabriel Pereira Nunes ◽  
Francisco Nunes Souza Neto ◽  
...  
Keyword(s):  
Fluoride Toothpaste ◽  
Sodium Trimetaphosphate ◽  
Enamel Demineralization ◽  
Demineralization Prevention

scholarly journals Influence of toothbrushing on enamel softening and abrasive wear of eroded bovine enamel: an in situ study

2006 ◽  
Vol 20 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Daniela Rios ◽  
Heitor Marques Honório ◽  
Ana Carolina Magalhães ◽  
Marília Afonso Rabelo Buzalaf ◽  
Regina Guenka Palma-Dibb ◽  
...  
Keyword(s):  
Abrasive Wear ◽  
The Other ◽  
In Situ Study ◽  
Significant Difference ◽  
The Mean ◽  
Student’S T ◽  
Bovine Enamel ◽  
Enamel Layer ◽  
Student’S T Test

This study assessed the surface softening and abrasive wear of eroded bovine enamel with or without the influence of toothbrushing. Five volunteers took part in this in situ study of 5 days. They wore acrylic palatal appliances containing 6 bovine enamel blocks divided in two rows with 3 blocks, which corresponded to the studied groups: erosion without toothbrushing (GI) and erosion with toothbrushing (GII). The blocks were subjected to erosion by immersion of the appliances in a cola drink for 10 minutes, 4 times a day. After that, no treatment was performed in one row (GI), whereas the other row was brushed (GII). The appliance was then replaced into the mouth. Enamel alterations were determined using profilometry and microhardness tests. Data were tested using paired Student’s t test (p < 0.05). The mean wear values (µm) and percentage of superficial microhardness change (%SMHC) were respectively: GI - 2.77 ± 1.21/91.61 ± 3.68 and GII - 3.80 ± 0.91/58.77 ± 11.47. There was a significant difference in wear (p = 0.001) and %SMHC (p = 0.001) between the groups. It was concluded that the wear was more pronounced when associated to toothbrushing abrasion. However, toothbrushing promoted less %SMHC due to the removal of the altered superficial enamel layer.

scholarly journals Effect of Incorporation of Remineralizing Agents into Bleaching Gels on the Microhardness of Bovine Enamel in situ

2014 ◽  
Vol 15 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Carlos Rocha Gomes Torres ◽  
Alessandra Bühler Borges ◽  
Carolina Anne Guimarães ◽  
Eduardo Bresciani ◽  
Carolina Judica Ramos ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Human Saliva ◽  
Mean Values ◽  
Bleaching Procedure ◽  
Demineralized Enamel ◽  
Before And After ◽  
Bovine Enamel ◽  
Bleaching Gels ◽  
Intraoral Devices

ABSTRACT Aim This study evaluated the effect of adding calcium or fluoride to 35% hydrogen peroxide (HP) bleaching gel and the effect of human saliva on the microhardness of sound and demineralized enamel, using an in situ model. Materials and methods Cylindrical bovine enamel specimens (3 × 2 mm) were divided into two groups (n = 30): sound enamel (SE) and demineralized enamel (DE). Each group was divided into three subgroups, according to the bleaching gel: 35% HP; 35% HP + calcium; 35% HP + fluoride. After bleaching therapy, the specimens were fixed to intraoral devices worn by 10 volunteers for 7 days. Surface enamel microhardness (SMH) was measured before and after bleaching procedures, and after 1 and 7 days of saliva exposure. Data were analyzed by Repeated Measures ANOVA (5%). Results The variable time resulted in significant differences for SE and DE groups (p = 0.001). For SE, significantly lower SMH was detected for control at post-bleaching period in comparison to the baseline and after 7 days. For DE, the lowest mean values were obtained before bleaching, and the addition of calcium to the peroxide significantly increased enamel SMH. The exposure to human saliva resulted in increased SMH. Conclusion The addition of potential remineralizing agents into bleaching gels might play an important role in maintaining the microhardness of sound enamel and in inducing remineralization of artificially demineralized enamel right after bleaching, and the remineralizing action of human saliva might minimize the deleterious effects of bleaching gels on enamel. Clinical significance The incorporation of calcium into HP bleaching gel might be beneficial for the initial phases of the bleaching procedure. How to cite this article Borges AB, Guimarães CA, Bresciani E, Ramos CJ, Borges ALS, Torres CRG. Effect of Incorporation of Remineralizing Agents into Bleaching Gels on the Microhardness of Bovine Enamel in situ. J Contemp Dent Pract 2014;15(2):195-201.

scholarly journals Influence of Calcium Phosphate and Apatite Containing Products on Enamel Erosion

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
A. Kensche ◽  
S. Pötschke ◽  
C. Hannig ◽  
G. Richter ◽  
W. Hoth-Hannig ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Oral Exposure ◽  
Ion Release ◽  
Pellicle Formation ◽  
Protective Properties ◽  
Ph Values ◽  
Mineral Components ◽  
Tooth Mousse ◽  
Bovine Enamel

For the purpose of erosion prevention the present study aimed to compare the efficacy of two biomimetic products and a fluoride solution to optimize the protective properties of the pellicle. After 1 min ofin situpellicle formation on bovine enamel slabs, 8 subjects adopted CPP-ACP (GC Tooth Mousse), a mouthwash with hydroxyapatite microclusters (Biorepair), or a fluoride based mouthwash (elmex Kariesschutz) for 1 min each. Afterwards, samples were exposed in the oral cavity for 28 min. Native enamel slabs and slabs exposed to the oral cavity for 30 min without any rinse served as controls. After oral exposure, slabs were incubated in HCl (pH values 2, 2.3, and 3) for 120 s and kinetics of calcium and phosphate release were measured photometrically; representative samples were evaluated by SEM and TEM. The physiological pellicle reduced demineralization at all pH values; the protective effect was enhanced by fluoride. The biomimetic materials also reduced ion release but their effect was less pronounced. SEM indicated no layer formation after use of the different products. However, TEM confirmed the potential accumulation of mineral components at the pellicle surface. The tested products improve the protective properties of thein situpellicle but not as effectively as fluorides.

scholarly journals Effects of fluoride and Aloe vera tooh gel in artificial white spot lesions in vitro

2016 ◽  
Vol 64 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Tânia Mara da SILVA ◽  
Beatriz Maria da FONSECA ◽  
Ana Luísa Leme Simões SALES ◽  
Priscila HOLLEBEN ◽  
Márcia Carneiro VALERA ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Aloe Vera ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Repeated Measures Anova ◽  
Ph Cycling ◽  
Knoop Microhardness ◽  
Bovine Enamel

ABSTRACT Objective: The aim of this study was to evaluate the effects of toothbrushing using a fluoride toothpaste and Aloe vera tooth gel on artificial white spot lesions through the Knoop microhardness (KHN) analysis. Methods: Sound bovine enamel samples (2 mm/diameter and 2 mm/depth) were prepared and immersed in artificial white spot lesion for 24 h. The preparation of artificial white spot lesions was performed by pH-cycling process. The samples were randomly divided into two groups (n=20), according the dentifrice used: containing fluoride (Colgate Total 12) or Aloe vera (Forever Bright Aloe Vera Toothgel). The top surface of samples was submitted to 10,000, 25,000, 50,000 and 100,000 brushing cycles (200 g load) in an automatic brushing machine with abrasive slurry. The KHN analysis were evaluated at baseline, after immersion in artificial white spot lesion and after 10,000, 25,000, 50,000 and 100,000 cycles of brushing. Data were analyzed by two-way repeated measures ANOVA and Tukey tests (p=0.05). Results: The KHN values significantly increased after brushing cycles compared to demineralized means. No significant differences showed for dentifrice factor (p=0.263). However, there were statistically significant differences between groups in cycles of brushing times (p=0.0001). Conclusion: The toothpastes (containing fluoride or Aloe vera) were effective in increasing the superficial microhardness of artificial white spot lesions.

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