scholarly journals Fibroblasts and Mesenchymal Stromal/Stem Cells Are Phenotypically Indistinguishable

2016 ◽  
Vol 136 (2) ◽  
pp. 85-97 ◽  
Author(s):  
Ryan A. Denu ◽  
Steven Nemcek ◽  
Debra D. Bloom ◽  
A. Daisy Goodrich ◽  
Jaehyup Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Collagen Type I ◽  
Type I ◽  
Human Mscs ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Phenotypic Characteristics ◽  
Human Fibroblast Strains ◽  
Stromal Stem Cells

Background/Aims: Human mesenchymal stromal/stem cells (MSCs), derived from many different tissues, are characterized by a fibroblast-like morphology, the expression of certain cell surface markers and their ability to differentiate into adipocytes, chondrocytes and osteoblasts. A number of studies have shown that MSCs share many characteristics with fibroblasts; however, there is no well-defined set of phenotypic characteristics that could distinguish between these 2 types of cells. Methods: We used 4 well-established human fibroblast strains from 3 different tissue sources and several human MSC strains from 2 different tissue sources to compare the phenotypic and immunological characteristics of these cells. Results: Fibroblast strains had a similar morphology to MSCs, expressed the same cell surface markers as MSCs and could also differentiate into adipocytes, chondrocytes and osteoblasts. Also, similar to MSCs, these fibroblasts were capable of suppressing T cell proliferation and modulating the immunophenotype of macrophages. We also show that MSCs deposit extracellular matrices of collagen type I and fibronectin, and express FSP1 in patterns similar to fibroblasts. Conclusions: Based on currently accepted definitions for cultured human MSCs and fibroblasts, we could not find any immunophenotypic property that could make a characteristic distinction between MSCs and fibroblasts.

scholarly journals Proteomic Definitions of Mesenchymal Stem Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Martin H. Maurer
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Adult Stem Cells ◽  
Cell Number ◽  
Human Mscs ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Protein Markers ◽  
Expression Of Genes

Mesenchymal stem cells (MSCs) are pluripotent cells isolated from the bone marrow and various other organs. They are able to proliferate and self-renew, as well as to give rise to progeny of at least the osteogenic, chondrogenic, and adipogenic lineages. Despite this functional definition, MSCs can also be defined by their expression of a distinct set of cell surface markers. In the current paper, studies investigating the proteome of human MSCs are reviewed with the aim to identify common protein markers of MSCs. The proteomic analysis of MSCs revealed a distinct set of proteins representing the basic molecular inventory, including proteins for (i) cell surface markers, (ii) the responsiveness to growth factors, (iii) the reuse of developmental signaling cascades in adult stem cells, (iv) the interaction with molecules of the extracellular matrix, (v) the expression of genes regulating transcription and translation, (vi) the control of the cell number, and (vii) the protection against cellular stress.

Neonatal stem cells exhibit specific characteristics in function, proliferation, and cellular signaling that distinguish them from their adult counterparts

2008 ◽  
Vol 294 (5) ◽  
pp. R1491-R1497 ◽  
Author(s):  
Troy A. Markel ◽  
Meijing Wang ◽  
Paul R. Crisostomo ◽  
Maiuxi C. Manukyan ◽  
Jeffrey A. Poynter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Local Inflammatory Response ◽  
Cell Counts ◽  
Marrow Mesenchymal Stem Cells ◽  
Growth Factor Production ◽  
Erk Inhibition ◽  
Different Levels

Stem cells may be a novel treatment modality for organ ischemia, possibly through beneficial paracrine mechanisms. Stem cells from older hosts have been shown to exhibit decreased function during stress. We therefore hypothesized that 1) neonatal bone marrow mesenchymal stem cells (nBMSCs) would produce different levels of IL-6, VEGF, and IGF-1 compared with adults (aBMSCs) when stimulated with TNF or LPS; 2) differences in cytokines would be due to distinct cellular characteristics, such as proliferation or pluripotent potential; and 3) differences in cytokines would be associated with differences in p38 MAPK and ERK signaling within nBMSCs. BMSCs were isolated from adult and neonatal mice. Cells were exposed to TNF or LPS with or without p38 or ERK inhibition. Growth factors were measured via ELISA, proliferation via daily cell counts, cell surface markers via flow cytometry, and pluripotent potential via alkaline phosphatase activity. nBMSCs produced lower levels of IL-6 and VEGF, but higher levels of IGF-1 under basal conditions, as well as after stimulation with TNF, but not LPS. Neonatal and adult BMSCs had similar pluripotent potentials and cell surface markers, but nBMSCs proliferated faster. Furthermore, p38 and ERK appeared to play a more substantial role in nBMSC cytokine and growth factor production. Neonatal stem cells may aid in decreasing the local inflammatory response during ischemia, and could possibly be expanded more rapidly than adult cells prior to therapeutic use.

Cell surface markers and exogenously induced PpIX in synovial mesenchymal stem cells

2015 ◽  
Vol 87 (11) ◽  
pp. 1001-1011 ◽  
Author(s):  
Jaroslav Denkovskij ◽  
Romualdas Rudys ◽  
Eiva Bernotiene ◽  
Mindaugas Minderis ◽  
Saulius Bagdonas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers

Phenotypic analysis of cell surface markers and gene expression of human mesenchymal stem cells and chondrocytes during monolayer expansion

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 513-526 ◽  
Author(s):  
Christel Cournil-Henrionnet ◽  
Céline Huselstein ◽  
Yun Wang ◽  
Laurent Galois ◽  
Didier Mainard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Human Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Phenotypic Analysis ◽  
Monolayer Expansion

Mesenchymal stem cells from adipose tissue which have been differentiated into chondrocytes in three-dimensional culture express lubricin

2011 ◽  
Vol 236 (11) ◽  
pp. 1333-1341 ◽  
Author(s):  
Giuseppe Musumeci ◽  
Debora Lo Furno ◽  
Carla Loreto ◽  
Rosario Giuffrida ◽  
Silvia Caggia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Collagen Type ◽  
Three Dimensional ◽  
3D Culture ◽  
Collagen Type I ◽  
Type I ◽  
Human Mscs ◽  
Alternative Source

The present study focused on the isolation, cultivation and characterization of human mesenchymal stem cells (MSCs) from adipose tissue and on their differentiation into chondrocytes through the NH ChondroDiff medium. The main aim was to investigate some markers of biomechanical quality of cartilage, such as lubricin, and collagen type I and II. Little is known, in fact, about the ability of chondrocytes from human MSCs of adipose tissue to generate lubricin in three-dimensional (3D) culture. Lubricin, a 227.5-kDa mucinous glycoprotein, is known to play an important role in articular joint physiology, and the loss of accumulation of lubricin is thought to play a role in the pathology of osteoarthritis. Adipose tissue is an alternative source for the isolation of multipotent MSCs, which allows them to be obtained by a less invasive method and in larger quantities than from other sources. These cells can be isolated from cosmetic liposuctions in large numbers and easily grown under standard tissue culture conditions. 3D chondrocytes were assessed by histology (hematoxylin and eosin) and histochemistry (Alcian blue and Safranin-O/fast green staining). Collagen type I, II and lubricin expression was determined through immunohistochemistry and Western blot. The results showed that, compared with control cartilage and monolayer chondrocytes showing just collagen type I, chondrocytes from MSCs (CD44-, CD90- and CD105- positive; CD45-, CD14- and CD34-negative) of adipose tissue grown in nodules were able to express lubricin, and collagen type I and II, indicative of hyaline cartilage formation. Based on the function of lubricin in the joint cavity and disease and as a potential therapeutic agent, our results suggest that MSCs from adipose tissue are a promising cell source for tissue engineering of cartilage. Our results suggest that chondrocyte nodules producing lubricin could be a novel biotherapeutic approach for the treatment of cartilage abnormalities.

scholarly journals Cell surface markers for the identification and study of human naive pluripotent stem cells

2018 ◽  
Vol 26 ◽  
pp. 36-43 ◽  
Author(s):  
Oliver Trusler ◽  
Ziyi Huang ◽  
Jacob Goodwin ◽  
Andrew L. Laslett
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Pluripotent Stem Cells ◽  
Surface Markers ◽  
Cell Surface Markers
Sign in / Sign up

Export Citation Format

Share Document