Cell surface markers and exogenously induced PpIX in synovial mesenchymal stem cells

2015 ◽  
Vol 87 (11) ◽  
pp. 1001-1011 ◽  
Author(s):  
Jaroslav Denkovskij ◽  
Romualdas Rudys ◽  
Eiva Bernotiene ◽  
Mindaugas Minderis ◽  
Saulius Bagdonas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers

Phenotypic analysis of cell surface markers and gene expression of human mesenchymal stem cells and chondrocytes during monolayer expansion

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 513-526 ◽  
Author(s):  
Christel Cournil-Henrionnet ◽  
Céline Huselstein ◽  
Yun Wang ◽  
Laurent Galois ◽  
Didier Mainard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Human Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Phenotypic Analysis ◽  
Monolayer Expansion

scholarly journals Flow Cytometric Characterization of Cell Surface Markers to Differentiate Between Fibroblasts and Mesenchymal Stem Cells of Different Origin

2021 ◽  
Author(s):  
Suzanne Sober ◽  
Homa Darmani ◽  
Dana Alhattab ◽  
Abdalla Awidi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Surface ◽  
Placental Tissue ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Wharton's Jelly ◽  
Different Origins

IntroductionIdentification and purification of mesenchymal stem cells (MSCs) expanded in culture for therapeutic use is crucial for improved yield and optimal results. Fibroblasts are the most common cell type in connective tissue and are commonly found as contaminants of MSC cultures, affecting cell yield and potentially causing tumor formation after cell transplantation. In the current study, we wished to identify cell surface markers that can differentiate MSCs of different origins from fibroblasts.Material and methodsMSCs were isolated from bone marrow, adipose tissue, Wharton’s jelly and placental tissue and fibroblasts were isolated from foreskin (as a negative control) in order to examine the differences in the expression of a panel of 14 different cell surface markers using multiplex flow cytometry.ResultsOur results indicate that the following markers could be useful in differentiating between fibroblasts and MSCs derived from: adipose tissue - CD79a, CD105, CD106, CD146, and CD271; Wharton’s jelly - CD14, CD56 and CD105; bone marrow - CD105, CD106, and CD146; placental tissue - CD14, CD105, and CD146. Furthermore, we found that, contradictory to previous studies, CD26 is not fibroblast-specific.ConclusionsIn conclusion, the results of our study indicate that cell surface markers may prove to be a useful tool in the discrimination between MSCs of different origins and fibroblasts and thus may be used to authenticate the identity of the isolated cells.

scholarly journals Proteomic Definitions of Mesenchymal Stem Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Martin H. Maurer
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Adult Stem Cells ◽  
Cell Number ◽  
Human Mscs ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Protein Markers ◽  
Expression Of Genes

Mesenchymal stem cells (MSCs) are pluripotent cells isolated from the bone marrow and various other organs. They are able to proliferate and self-renew, as well as to give rise to progeny of at least the osteogenic, chondrogenic, and adipogenic lineages. Despite this functional definition, MSCs can also be defined by their expression of a distinct set of cell surface markers. In the current paper, studies investigating the proteome of human MSCs are reviewed with the aim to identify common protein markers of MSCs. The proteomic analysis of MSCs revealed a distinct set of proteins representing the basic molecular inventory, including proteins for (i) cell surface markers, (ii) the responsiveness to growth factors, (iii) the reuse of developmental signaling cascades in adult stem cells, (iv) the interaction with molecules of the extracellular matrix, (v) the expression of genes regulating transcription and translation, (vi) the control of the cell number, and (vii) the protection against cellular stress.

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