Influence of Toothbrushing on the Antierosive Effect of Film-Forming Agents

2016 ◽  
Vol 50 (2) ◽  
pp. 104-110 ◽  
Author(s):  
Taís Scaramucci ◽  
Samira Helena João-Souza ◽  
Frank Lippert ◽  
George J. Eckert ◽  
Idalina V. Aoki ◽  
...  
Keyword(s):  
Protective Effect ◽  
Sodium Fluoride ◽  
Stannous Chloride ◽  
Negative Control ◽  
Deionized Water ◽  
The Other ◽  
Surface Loss ◽  
Film Forming ◽  
Better Than

This study evaluated the influence of toothbrushing on the antierosive effect of solutions containing sodium fluoride (225 ppm/F), stannous chloride (800 ppm/Sn), sodium linear polyphosphate (2%/LPP), and their combinations, and deionized water as negative control (C). Solutions were tested in a 5-day erosion-remineralization-abrasion cycling model, using enamel and dentin specimens (n = 8). Erosion was performed 6 times/day for 5 min, exposure to the test solutions 3 times/day for 2min, and toothbrushing (or not) with toothpaste slurry 2 times/day for 2 min (45 strokes). Surface loss (SL) was determined by noncontact profilometry. Data were analyzed using three-way ANOVA (α = 0.05). Brushing caused more SL than no brushing for enamel (mean ± SD, in micrometers: 52.7 ± 6.6 and 33.0 ± 4.5, respectively), but not for dentin (28.2 ± 1.9 and 26.6 ± 1.8, respectively). For enamel without brushing, F+LPP+Sn showed the lowest SL (23.8 ± 3.4), followed by F+Sn (30.6 ± 4.9) and F+LPP (31.7 ± 1.7), which did not differ from each other. No differences were found between the other groups and C (37.8 ± 2.1). When brushing, F+LPP+Sn exhibited the lowest SL (36.7 ± 2.4), not differing from F+LPP (39.1 ± 1.8). F, F+Sn and LPP+Sn were similar (46.7 ± 2.9, 42.1 ± 2.8 and 45.3 ± 4.6, respectively) and better than C (52.7 ± 4.3). Sn (55.0 ± 2.4) and LPP (51.0 ± 4.3) did not differ from C. For dentin, neither groups differed from C, regardless of brushing. In conclusion, toothbrushing did not affect the antierosive effect of F+Sn, F+LPP and F+LPP+Sn on enamel, although overall it led to more erosion than nonbrushing. F and LPP+Sn showed a protective effect only under brushing conditions, whereas Sn and LPP did not exhibit any protection. For dentin, neither toothbrushing nor the test solutions influenced the development of erosion.

Protective Effect of Solutions Containing Polymers Associated with Fluoride and Stannous Chloride on Hydroxyapatite Dissolution

2021 ◽  
pp. 1-8
Author(s):  
Marina Gullo Augusto ◽  
Tamires Maria de Andrade Santos ◽  
Taís Scaramucci ◽  
Idalina Vieira Aoki ◽  
Carlos Rocha Gomes Torres ◽  
...  
Keyword(s):  
Protective Effect ◽  
Sodium Fluoride ◽  
Stannous Chloride ◽  
Deionized Water ◽  
Lower Amount ◽  
Citric Acid Solution ◽  
Solution Ph ◽  
Methacrylate Copolymer ◽  
Total Reaction Time ◽  
Hydroxyapatite Crystals

This study investigated the protective effect of experimental solutions containing 4 polymers (polyoxirane, hydroxypropylmethylcellulose [HPMC], pectin, and an amino methacrylate copolymer [AMC]) in 2 concentrations (low and high) associated or not with sodium fluoride (F; 225 ppm F<sup>–</sup>) or sodium fluoride plus stannous chloride (FS; 800 ppm Sn<sup>2+</sup>) on the dissolution of hydroxyapatite crystals (HA). Deionized water was the control. The pretreated HA was added to a 0.3% citric acid solution (pH 3.8). An automatic titrant machine added aliquots of 0.1 N HCl at a rate of 28 μL/min, in a total reaction time of 5 min. Groups were compared with 2-way ANOVA and Tukey’s test, and concentrations with Student <i>t</i> test (5%). The zeta potential of the HA treated with the solutions was measured. Significant differences were found for both factors and interaction (<i>p</i> &#x3c; 0.0001). The treatments with F and FS solutions resulted in a lower amount of dissolved HA than the control. Among the polymers’ solutions, only AMC was able to reduce the amount of dissolved HA, changing the surface charge of HA to positive. AMC improved the protective effect of F, but it did not affect FS. Polyoxirane and HPMC reduced the protective potential of the FS solution. No differences were found between the concentrations of the polymers. It was concluded that F and FS reduced the amount of dissolved HA. The protective effect of the experimental solutions against HA dissolution was polymer dependent. The F effect was enhanced by its combination with AMC, but the protection of FS was impaired by polyoxirane and HPMC.

Anti-Erosive Effect of Solutions Containing Sodium Fluoride, Stannous Chloride, and Selected Film-Forming Polymers

2018 ◽  
Vol 53 (3) ◽  
pp. 305-313 ◽  
Author(s):  
Sávio J.C. Bezerra ◽  
Samira H. João-Souza ◽  
Idalina V. Aoki ◽  
Alessandra B. Borges ◽  
Anderson T. Hara ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Stannous Chloride ◽  
Human Saliva ◽  
Negative Control ◽  
Zeta Potentials ◽  
Film Forming ◽  
All Solutions ◽  
Propylene Glycol Alginate ◽  
Erosive Effect ◽  
Hydroxyapatite Crystals

The aim of this study was to evaluate the anti-erosive effect of solutions containing sodium fluoride (F: 225 ppm F–), stannous chloride (Sn: 800 ppm Sn2+), and some film-forming polymers (Gantrez: Poly [methylvinylether-alt-maleic anhydride]; PGA: propylene glycol alginate; Plasdone: poly[vinylpyrrolidone]; and CMC: carboxymethylcellulose). Solutions were tested in an erosion-remineralization cycling model, using enamel and dentin specimens (n = 10, for each substrate). Distilled water was the negative control. Cycling consisted of 120 min immersion in human saliva, 5 min in 0.3% citric acid solution, and 120 min of exposure to human saliva, 4×/day, for 5 days. Treatment with solutions (pH = 4.5) was carried out 2×/day, for 2 min. Surface loss (SL) was evaluated with optical profilometry. Zeta potential of hydroxyapatite crystals was determined after treatment with the solutions. Data were statistically analyzed (α = 0.05). For enamel, all polymers showed significantly lower SL (in µm) than the control (11.09 ± 0.94), except PGA (10.15 ± 1.25). PGA significantly improved the protective effect of F (4.24 ± 0.97 vs. 5.64 ± 1.60, respectively). None of the polymers increased the protection of F+Sn (5.13 ± 0.78). For dentin, only Gantrez (11.40 ± 0.97) significantly reduced SL when compared with the negative control (12.76 ± 0.75). No polymer was able to enhance the effect of F (6.28 ± 1.90) or F+Sn (7.21 ± 1.13). All fluoridated solutions demonstrated significantly lower SL values than the control for both substrates. Treatment of hydroxyapatite nanoparticles with all solutions resulted in more negative zeta potentials than those of the control, except Plasdone, PGA, and F+Sn+PGA, the latter two presenting the opposite effect. In conclusion, Gantrez, Plasdone, and CMC exhibited an anti-erosive effect on enamel. PGA increased the protection of F. For dentin, only Gantrez reduced erosion.

scholarly journals Caries infiltrant combined with conventional adhesives for sealing sound enamel in vitro

2013 ◽  
Vol 83 (5) ◽  
pp. 858-863 ◽  
Author(s):  
Enver Yetkiner ◽  
Florian Just Wegehaupt ◽  
Rengin Attin ◽  
Thomas Attin
Keyword(s):  
Protective Effect ◽  
In Vitro Study ◽  
Negative Control ◽  
Protective Measures ◽  
Low Viscosity ◽  
Monomer Content ◽  
Bovine Enamel ◽  
Mineral Loss ◽  
Better Than

ABSTRACT Objective: To test the null hypothesis that combining low-viscosity caries infiltrant with conventional adhesive resins would not improve sealing of sound enamel against demineralization in vitro. Materials and Methods: Bovine enamel discs (N  =  60) with diameter of 3 mm were randomly assigned to six groups (n  =  10). The discs were etched with 37% phosphoric acid for 30 seconds and treated with resins of different monomer content forming the following groups: (1) Icon (DMG), (2) Transbond XT Primer (3M ESPE), (3) Heliobond (Ivoclar Vivadent), (4) Icon + Transbond XT Primer, and (5) Icon + Heliobond. Untreated etched samples served as the negative control. Specimens were subjected to demineralization by immersion in hydrochloric acid (pH 2.6) for 80 hours. Calcium dissolution into the acid was assessed by colorimetric analysis using Arsenazo III method at 16-hour intervals. Groups presenting high protection against demineralization were subjected to further acidic challenge for 15 days with calcium measurements repeated at 24-hour intervals. Data were analyzed by Kruskal-Wallis test and Mann-Whitney U-test. Results: Untreated specimens showed the highest amount of demineralization. Icon and Transbond XT primer decreased the mineral loss significantly compared to the control. Heliobond performed significantly better than both Icon and Transbond XT primer. Combination of Icon both with Transbond XT primer or Heliobond served as the best protective measures and maintained the protective effect for the additional 15-day acidic challenge. Conclusions: Within the limitations of this in vitro study, it could be concluded that the use of low-viscosity caries infiltrant prior to application of the tested conventional adhesives increases their protective effect against demineralization.

Enhancing the Anti-Erosive Properties of Fluoride and Stannous with the Polymer Carbopol

2020 ◽  
Vol 54 (3) ◽  
pp. 250-257 ◽  
Author(s):  
Daniele Mara da Silva Ávila ◽  
Marina Gullo Augusto ◽  
Rayssa Ferreira Zanatta ◽  
Tais Scaramucci ◽  
Idalina Vieira Aoki ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Stannous Chloride ◽  
In Vitro Study ◽  
Human Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Bovine Enamel ◽  
Erosion Protection ◽  
Erosive Effect

This in vitro study investigated whether Carbopol 980 polymer could potentiate the anti-erosive effect of solutions containing sodium fluoride (F) and sodium fluoride associated with stannous chloride (FS). The dissolution of hydroxyapatite treated with the experimental solutions (F [500 ppm F–], F + Carbopol [0.1%], FS [500 ppm F– + 800 ppm Sn2+], FS + Carbopol) was evaluated. Deionized water was the negative control, and a commercial mouth rinse (AmF/NaF/SnCl2; 500 ppm F + 800 ppm Sn2+; Elmex® Erosion Protection) was the positive control. The solutions were also evaluated in an erosion-rehardening protocol, with two treatments per day, using bovine enamel specimens (n = 15) and human saliva. The acid challenge was performed using 0.3% citric acid (pH 2.6) for 2 min. Microhardness was measured at different times: baseline, after the first erosive challenge, after treatment, and after the second erosive challenge. Based on microhardness values, the demineralization, rehardening, and protective potentials were calculated. The alkali-soluble fluoride on enamel surfaces was also measured. Data were analyzed using ANOVA and Tukey tests (α = 0.05). Groups treated with FS + Carbopol showed the lowest hydroxyapatite dissolution and the highest rehardening and protective potentials. The measurement of alkali-soluble fluoride on enamel surfaces was also higher in the FS + Carbopol group. Carbopol was able to significantly increase the protective effect of the fluoridated solutions in addition to optimizing the adsorption of fluoride on the enamel surface.

scholarly journals Comparative Effects of CPP-ACP and Xylitol F-Varnishes on the Reduction of Tooth Erosion and Its Progression

2020 ◽  
Vol 31 (6) ◽  
pp. 664-672
Author(s):  
Adílis Alexandria ◽  
Ana Maria Gondim Valença ◽  
Lúcio Mendes Cabral ◽  
Lucianne Cople Maia
Keyword(s):  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Deionized Water ◽  
3D Images ◽  
Significance Level ◽  
Tooth Structure ◽  
Varnish Layer ◽  
One Way Anova ◽  
Better Than

Abstract Evaluated the effect of CPP-ACP/NaF and xylitol/NaF varnishes in reduce erosion and progression of erosion. Forty enamel blocks were divided into four groups (n=10): G1=CPP-ACP/NaF varnish (MI varnishTM); G2=xylitol/NaF varnish (Profluorid®); G3=NaF varnish (Duraphat®, positive control) and G4=deionized water (MilliQ®, negative control). Samples were immersed in Sprite ZeroTM (pH 2.58, 4x/day, 3 days), in between immersions, the specimens stayed in artificial saliva. After 3 days of erosion, the eroded area was divided in two (half of one received an additional varnish layer while the other half repeated the same 3-day erosion cycle). The 3D, non-contact profilometry technique was used to determinate tooth structure loss (TSL) and surface roughness (SR). Scanning electron microscopy (SEM) and 3D images were utilized to evaluate the topography of the samples. Mann-Whitney, one-way ANOVA and Tukey tests were used (significance level of 0.05%). SEM and 3D images were descriptively evaluated. After 3 or 6 days of erosion, all tested varnishes were better than G4 (p<0.05) for TSL and SR. In addition, G1 had lower values for TSL than G3 (p<0.05) after 3 days of erosion. Under SEM and 3D images observation, all groups presented porosity, irregularities and depressions on the surface enamel after 3 and 6 days of erosion, more pronounced in G4. An application of topical NaF varnishes was effective in reducing TSL and enamel roughness after erosion challenges, being the CCP-ACP/NaF varnish more effective than NaF varnish and water after 3 days of erosion.

Closing the GAP—A 5Å Scanning Microscope

1969 ◽  
Vol 27 ◽  
pp. 6-7
Author(s):  
A. V. Crewe
Keyword(s):  
Normal Form ◽  
The Other ◽  
Resolving Power ◽  
Scanning Microscope ◽  
Conventional Microscope ◽  
Other Hand ◽  
Closing The Gap ◽  
Thermal Sources ◽  
Better Than ◽  
Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

MUNDANE MIRACLES IN AWADH. ENCOUNTERS WITH THE REVEALED AND THE HIDDEN

2020 ◽  
pp. 130-140
Author(s):  
Maxim B. Demchenko ◽  
Keyword(s):  
The Other ◽  
Local People ◽  
Natural Phenomena ◽  
Mughal Empire ◽  
The Public ◽  
British Raj ◽  
The Subject ◽  
Other World ◽  
Better Than

The sphere of the unknown, supernatural and miraculous is one of the most popular subjects for everyday discussions in Ayodhya – the last of the provinces of the Mughal Empire, which entered the British Raj in 1859, and in the distant past – the space of many legendary and mythological events. Mostly they concern encounters with inhabitants of the “other world” – spirits, ghosts, jinns as well as miraculous healings following magic rituals or meetings with the so-called saints of different religions (Hindu sadhus, Sufi dervishes),with incomprehensible and frightening natural phenomena. According to the author’s observations ideas of the unknown in Avadh are codified and structured in Avadh better than in other parts of India. Local people can clearly define if they witness a bhut or a jinn and whether the disease is caused by some witchcraft or other reasons. Perhaps that is due to the presence in the holy town of a persistent tradition of katha, the public presentation of plots from the Ramayana epic in both the narrative and poetic as well as performative forms. But are the events and phenomena in question a miracle for the Avadhvasis, residents of Ayodhya and its environs, or are they so commonplace that they do not surprise or fascinate? That exactly is the subject of the essay, written on the basis of materials collected by the author in Ayodhya during the period of 2010 – 2019. The author would like to express his appreciation to Mr. Alok Sharma (Faizabad) for his advice and cooperation.

Temperature Effects during Endodormancy Induction on Subsequent Anthesis and Growth of Peach Trees

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 452c-452 ◽  
Author(s):  
Schuyler D. Seeley ◽  
Raymundo Rojas-Martinez ◽  
James Frisby
Keyword(s):  
Temperature Effects ◽  
Shoot Length ◽  
The Other ◽  
Nighttime Temperatures ◽  
Peach Trees ◽  
Better Than

Mature peach trees in pots were treated with nighttime temperatures of –3, 6, 12, and 18 °C for 16 h and a daytime temperature of 20 °C for 8 h until the leaves abscised in the colder treatments. The trees were then chilled at 6 °C for 40 to 70 days. Trees were removed from chilling at 40, 50, 60, and 70 days and placed in a 20 °C greenhouse under increasing daylength, spring conditions. Anthesis was faster and shoot length increased with longer chilling treatments. Trees exposed to –3 °C pretreatment flowered and grew best with 40 days of chilling. However, they did not flower faster or grow better than the other treatments with longer chilling times. There was no difference in flowering or growth between the 6 and 12 °C pretreatments. The 18 °C pretreatment resulted in slower flowering and very little growth after 40 and 50 days of chilling, but growth was comparable to other treatments after 70 days of chilling.

Nglyc: A Random Forest Method for Prediction of N-Glycosylation Sites in Eukaryotic Protein Sequence

2020 ◽  
Vol 27 (3) ◽  
pp. 178-186 ◽  
Author(s):  
Ganesan Pugalenthi ◽  
Varadharaju Nithya ◽  
Kuo-Chen Chou ◽  
Govindaraju Archunan
Keyword(s):  
Random Forest ◽  
Protein Sequence ◽  
Glycosylation Site ◽  
Computational Method ◽  
The Other ◽  
Eukaryotic Protein ◽  
Random Forest Method ◽  
Glycosylation Sites ◽  
Human And Mouse ◽  
Better Than

Background: N-Glycosylation is one of the most important post-translational mechanisms in eukaryotes. N-glycosylation predominantly occurs in N-X-[S/T] sequon where X is any amino acid other than proline. However, not all N-X-[S/T] sequons in proteins are glycosylated. Therefore, accurate prediction of N-glycosylation sites is essential to understand Nglycosylation mechanism. Objective: In this article, our motivation is to develop a computational method to predict Nglycosylation sites in eukaryotic protein sequences. Methods: In this article, we report a random forest method, Nglyc, to predict N-glycosylation site from protein sequence, using 315 sequence features. The method was trained using a dataset of 600 N-glycosylation sites and 600 non-glycosylation sites and tested on the dataset containing 295 Nglycosylation sites and 253 non-glycosylation sites. Nglyc prediction was compared with NetNGlyc, EnsembleGly and GPP methods. Further, the performance of Nglyc was evaluated using human and mouse N-glycosylation sites. Results: Nglyc method achieved an overall training accuracy of 0.8033 with all 315 features. Performance comparison with NetNGlyc, EnsembleGly and GPP methods shows that Nglyc performs better than the other methods with high sensitivity and specificity rate. Conclusion: Our method achieved an overall accuracy of 0.8248 with 0.8305 sensitivity and 0.8182 specificity. Comparison study shows that our method performs better than the other methods. Applicability and success of our method was further evaluated using human and mouse N-glycosylation sites. Nglyc method is freely available at https://github.com/bioinformaticsML/ Ngly.

pLoc_bal-mEuk: Predict Subcellular Localization of Eukaryotic Proteins by General PseAAC and Quasi-balancing Training Dataset

2019 ◽  
Vol 15 (5) ◽  
pp. 472-485 ◽  
Author(s):  
Kuo-Chen Chou ◽  
Xiang Cheng ◽  
Xuan Xiao
Keyword(s):  
Drug Development ◽  
Subcellular Localization ◽  
Basic Research ◽  
The Other ◽  
Training Dataset ◽  
Sequence Information ◽  
Eukaryotic Proteins ◽  
Validation Tests ◽  
User Friendly ◽  
Better Than

<P>Background/Objective: Information of protein subcellular localization is crucially important for both basic research and drug development. With the explosive growth of protein sequences discovered in the post-genomic age, it is highly demanded to develop powerful bioinformatics tools for timely and effectively identifying their subcellular localization purely based on the sequence information alone. Recently, a predictor called “pLoc-mEuk” was developed for identifying the subcellular localization of eukaryotic proteins. Its performance is overwhelmingly better than that of the other predictors for the same purpose, particularly in dealing with multi-label systems where many proteins, called “multiplex proteins”, may simultaneously occur in two or more subcellular locations. Although it is indeed a very powerful predictor, more efforts are definitely needed to further improve it. This is because pLoc-mEuk was trained by an extremely skewed dataset where some subset was about 200 times the size of the other subsets. Accordingly, it cannot avoid the biased consequence caused by such an uneven training dataset. </P><P> Methods: To alleviate such bias, we have developed a new predictor called pLoc_bal-mEuk by quasi-balancing the training dataset. Cross-validation tests on exactly the same experimentconfirmed dataset have indicated that the proposed new predictor is remarkably superior to pLocmEuk, the existing state-of-the-art predictor in identifying the subcellular localization of eukaryotic proteins. It has not escaped our notice that the quasi-balancing treatment can also be used to deal with many other biological systems. </P><P> Results: To maximize the convenience for most experimental scientists, a user-friendly web-server for the new predictor has been established at http://www.jci-bioinfo.cn/pLoc_bal-mEuk/. </P><P> Conclusion: It is anticipated that the pLoc_bal-Euk predictor holds very high potential to become a useful high throughput tool in identifying the subcellular localization of eukaryotic proteins, particularly for finding multi-target drugs that is currently a very hot trend trend in drug development.</P>

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