ACE Insertion/Deletion Polymorphism Associated with Caries in Permanent but Not Primary Dentition in Czech Children

2016 ◽  
Vol 50 (2) ◽  
pp. 89-96 ◽  
Author(s):  
Petra Borilova Linhartova ◽  
Jakub Kastovsky ◽  
Michaela Bartosova ◽  
Kristina Musilova ◽  
Lenka Zackova ◽  
...  
Keyword(s):  
Dental Caries ◽  
Primary Dentition ◽  
Caries Experience ◽  
Healthy Children ◽  
Czech Population ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
Gender Based ◽  
Control Study

Objective: Dental caries is a multifactorial, infectious disease where genetic predisposition plays an important role. Insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) has very recently been associated with caries in Polish children. The aim of this study was to analyze ACE I/D polymorphism in a group of caries-free children versus subjects affected by dental caries in the Czech population. Materials and Methods: In this case-control study, 182 caries-free children (with decayed/missing/filled teeth, DMFT = 0), 561 subjects with dental caries (DMFT ≥1) aged 13-15 years and 220 children aged 2-6 years with early childhood caries (ECC, dmft ≥1) were included. Genotype determination of ACE I/D polymorphism in intron 16 was based on the TaqMan method. Results: Although no significant differences in the allele or genotype frequencies between the caries-free children and those affected by dental caries were observed, statistically significant differences between the children with DMFT = 0 and the subgroup of 179 patients with high caries experience (DMFT ≥4; p < 0.01 and p < 0.05, respectively) were detected. The comparison of DD versus II+ID genotype frequencies between the patients with DMFT ≥1 or DMFT ≥4 and healthy children also showed significant differences (31.5% or 35.6% vs. 23.6%, p < 0.05 or p < 0.01, respectively). A gender-based analysis identified a significant difference in the DD versus II+ID genotype frequencies only in girls (p < 0.05). In contrast, no significant association of ACE I/D polymorphism with ECC in young children was found (p > 0.05). Conclusions:ACE I/D polymorphism may be associated with caries in permanent but not primary dentition, especially in girls in the Czech population.

Dental Caries, Developmental Defects of Enamel and Enamel Microhardness Associated with Genetic Polymorphisms in the RANK/RANKL/OPG System

2020 ◽  
Vol 44 (1) ◽  
pp. 35-40
Author(s):  
E Calvano Küchler ◽  
C Maschietto Pucinelli ◽  
K Carpio Horta ◽  
R Assed Bezerra da Silva ◽  
M de Castro Costa ◽  
...  
Keyword(s):  
Dental Caries ◽  
Genetic Polymorphisms ◽  
Primary Dentition ◽  
Caries Experience ◽  
Chi Square ◽  
Developmental Defects ◽  
Allele Distribution ◽  
Chain Reactions ◽  
Significant Difference ◽  
Developmental Defects Of Enamel

Purpose: Recent studies have suggested that disruptions in the RANKL/RANK/OPG system might be involved in enamel conditions. The aim of this study was to test whether genetic polymorphisms in RANK, RANKL and OPG are associated with dental caries, developmental defects of enamel (DDE) and enamel microhardness. Study design: Saliva samples were collected from two subsets for the purpose of genomic DNA extraction. In the first subset, composed of 248 children, dental caries and DDE were evaluated during their clinical examination. In the second subset, composed of 72 children, enamel samples from the buccal surface of primary teeth were used for enamel microhardness analysis. Genetic polymorphisms in RANK, RANKL and OPG were genotyped by real-time polymerase chain reactions in all samples from both populations. The chi-square test was used for dental caries and DDE analysis while, one-way ANOVA with Tukey's post-test was used for microhardness analysis. Hardy-Weinberg equilibrium was also calculated. The established alpha was 5%. Results: Caries experience analysis demonstrated a statistically-significant difference for OPG allele distribution in primary dentition (p=0.033). The studied polymorphisms in RANK, RANKL and OPG were not associated with DDE or enamel microhardness (p&gt;0.05). Conclusion: The genetic polymorphism rs2073618 in OPG is associated with dental caries experience in primary dentition.

Lack of Association between BMP2/DLX3 Gene Polymorphisms and Dental Caries in Primary and Permanent Dentitions

2017 ◽  
Vol 51 (6) ◽  
pp. 590-595 ◽  
Author(s):  
Jakub Kastovsky ◽  
Petra Borilova Linhartova ◽  
Kristina Musilova ◽  
Lenka Zackova ◽  
Martina Kukletova ◽  
...  
Keyword(s):  
Early Childhood ◽  
Dental Caries ◽  
Gene Polymorphisms ◽  
Early Childhood Caries ◽  
Primary Teeth ◽  
Permanent Dentition ◽  
Caries Experience ◽  
Czech Population ◽  
Genotype Frequencies ◽  
Childhood Caries

The aim of this study was to analyze the association between BMP2 (rs1884302) and DLX3 (rs2278163) gene polymorphisms and dental caries in primary and permanent dentitions. A total of 914 subjects were genotyped by the TaqMan methods: 176 caries-free children (with Decayed/Missing/Filled Teeth, DMFT = 0), 542 patients with dental caries in permanent dentition (DMFT ≥1), 83 caries-free children with primary teeth (with decayed/missing/filled teeth, dmft = 0), and 113 children with early childhood caries (ECC, dmft ≥1). There were no significant differences in allele/genotype frequencies between patients with caries in permanent dentition/ECC and caries-free children or between patients with very low (DMFT = 0-2), low (DMFT = 3-5), moderate (DMFT = 6-8), or high (DMFT ≥9) caries experience. Variability in BMP2 and DLX3 was not associated with caries in the Czech population.

Oral Health Status in Relation to Nutritional Status among 9 years old school Children in Dewanyiah City/Iraq

2019 ◽  
Vol 14 (1) ◽  
pp. 80
Author(s):  
Dr. Zahraa Ali Al-Awadi ◽  
Dr. Baydaa Hussien Hussien
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Nutritional Status ◽  
Oral Health Status ◽  
Health Condition ◽  
Primary Dentition ◽  
Anthropometric Measurement ◽  
Oral Diseases ◽  
Gingival Health ◽  
Significant Difference

Background: Although they are not life threatening, dental caries and periodontaldisease are the most predominant and widely spread oral diseases throughout theworld. The aims of the study included the investigation of the prevalence andseverity of dental caries, gingivitis and dental plaque in relation to gender,furthermore, nutritional status was assessed in relation to oral health condition(dental caries).Materials and Methods: This oral health survey was conducted among primaryschool children aged 9 years old in Dewanyiah city in Iraq. The total samplecomposed of 600 child (320 males and 280 females) selected randomly fromdifferent school in Dewanyiah city. Diagnosis of dental caries was according tothe criteria described by WHO (1987). Plaque index of Silness and Loe (1964)was used for plaque assessment, gingival index of Loe and Silness (1963) wasfollowed for recording gingival health condition. Nutritional status was assessedaccording to body mass index (BMI) indicator using anthropometric measurement(height and weight).Results: Results showed that the prevalence of dental caries was 85% for 9 year-oldschool children. Regarding primary and permanent dentition, dental caries washigher among females compared to males with statistically significant difference(P<0.05) for primary dentition, on the other hand, males showed higher values offilled surfaces compared to females with statistically significant difference(P<0.05) for primary dentition and highly significant difference (P<0.01) forpermanent dentition. Finding of this study revealed that 100% of the children hadgingival inflammation. Furthermore, the values of plaque and gingival indiceswere higher among males compared to females with statistically highly significantdifferences (P<0.01). In current study, the prevalence of malnutrition described bythe BMI indicator was 5.3%. For total samples no significant difference wasrecorded in dmfs /DMFS values among wasting and well nourished children(P>0.05).Conclusion: A high prevalence of dental caries and gingivitis were recorded.Improvement in the prevention educational programs is needed among schoolchildren.

MMP20 rs1784418 Protects Certain Populations against Caries

2016 ◽  
Vol 51 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Arnôldo V.A. Filho ◽  
Merilene S. Calixto ◽  
Kathleen Deeley ◽  
Neide Santos ◽  
Aronita Rosenblatt ◽  
...  
Keyword(s):  
Risk Factor ◽  
Dental Caries ◽  
Dna Extraction ◽  
Meta Analysis ◽  
Primary Dentition ◽  
Analytic Approach ◽  
Caries Experience ◽  
Statistical Approaches

This work aimed to further evaluate the association of MMP20 rs1784418 C>T and dental caries experience with the hypothesis that MMP20 rs1784418 C>T is a risk factor for dental caries. 184 children 4-7 years of age had their caries experience determined and buccal cheek swabs collected for DNA extraction to test for association with the MMP20 rs1784418 C>T using standard statistical approaches. A meta-analytic approach was also implemented to compile previous discrepant reports of the same association. We found an association between MMP20 rs1784418 C>T and dental caries experience in primary dentition (p = 0.01). The meta-analysis showed that this association appears to favor individuals born in Brazil and not Turkey. MMP20 rs1784418 C>T appears to protect against dental caries, but its effects are likely to be more marked in certain populations.

scholarly journals THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

2020 ◽  
pp. 20-23
Author(s):  
Sneha Upadhyay ◽  
Jyoti Bhavthankar ◽  
Mandakini Mandale ◽  
Nivedita Kaorey
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cross Sectional Study ◽  
Control Group ◽  
Healthy Children ◽  
Sectional Study ◽  
Cross Sectional ◽  
Asthmatic Children ◽  
Significant Difference ◽  
The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

scholarly journals Redefining the Phenotype of Dental Caries

2018 ◽  
Vol 52 (4) ◽  
pp. 263-271 ◽  
Author(s):  
Megan Weber ◽  
Jenny Bogstad Søvik ◽  
Aida Mulic ◽  
Kathleen Deeley ◽  
Anne B. Tveit ◽  
...  
Keyword(s):  
Dental Caries ◽  
Genetic Variants ◽  
Public Health Problem ◽  
Recessive Model ◽  
Primary Dentition ◽  
Caries Experience ◽  
Major Public Health Problem ◽  
Cross Sectional ◽  
Vast Number ◽  
Kallikrein 4

Dental caries is a multifactorial infectious disease and a major public health problem estimated to affect 60-90% of school children as well as a vast number of adults. The aim of this work was to define patterns of progression of the disease based on longitudinal data in contrast to using a cross-sectional assessment. dmft/DMFT scores were collected at ages 5, 12, 14, 16, 17, and 18 from 876 individuals. We tested our newly defined phenotypes for association with genetic variants in genes shown to be associated with caries. We generated genotyping data using Taqman chemistry in markers of genes involved in processes such as enamel formation and salivary contributions. Kallikrein 4 (KLK4) was found to show a significant association with the created phenotypes (p = 0.0008 in a recessive model for low caries experience in the primary dentition vs. high caries experience in the primary dentition, and p = 0.0004 in a recessive model for caries free primary dentition vs. high caries experience in the primary dentition).

Isolated itching of external auditory canal: clinicopathological study with immunohistochemical determination of antimicrobial peptides

2010 ◽  
Vol 125 (3) ◽  
pp. 227-230 ◽  
Author(s):  
B Acar ◽  
G Güler Simsek ◽  
S Oguztuzun ◽  
M Zaim ◽  
R Murat Karasen
Keyword(s):  
Antimicrobial Peptides ◽  
External Auditory Canal ◽  
Case Control Study ◽  
Case Control ◽  
Biopsy Specimens ◽  
Significant Difference ◽  
Skin Biopsies ◽  
Control Study ◽  
Prospective Case Control Study

AbstractObjective:This study aimed to demonstrate the histological and immunohistological features of skin biopsy specimens from patients complaining of isolated itching of the external auditory canal.Materials and methods:A prospective, case–control study was performed of 24 patients undergoing evaluation for contact dermatitis of the external auditory canal, and 24 controls. Skin biopsies were examined histologically by a single, blinded dermatopathologist, using light microscopy, to determine histopathological characteristics. The immunohistological presence of the antimicrobial peptides human β-defensin-3 and LL-37 cathelicidin was also assessed. Findings for patients and controls were compared.Results:There was a statistically significant difference in the degree of inflammation, comparing patients and controls (p < 0.05). There was no significant difference in the presence of spongiotic changes, comparing patients and controls (p > 0.05). Furthermore, the patients' skin biopsies did not show pronounced expression of human β-defensin-3 or LL-37 cathelicidin.Conclusion:Histological and immunohistological examination of skin biopsies from cases of isolated itching of the external auditory canal did not support a diagnosis of dermatitis.

scholarly journals Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

2017 ◽  
Vol 32 (2) ◽  
pp. 94
Author(s):  
Brunah De Oliveira Buche ◽  
Bruno Gusso ◽  
Maria Fernanda Torres ◽  
Mariana Dalledone ◽  
Fernanda Mara Paiva Bertoli ◽  
...  
Keyword(s):  
Oral Health ◽  
Statistical Tests ◽  
Human Saliva ◽  
Caries Experience ◽  
Healthy Children ◽  
Health Project ◽  
Spss Software ◽  
Level Of Significance ◽  
Saliva Flow ◽  
Control Study

OBJECTIVE: Human saliva has numerous components that are important for maintaining oral health. We investigated the association between iron levels in saliva in children and their caries experience.METHODS: We conducted a case-control study that included 186 healthy children, aged 11-14 years. The decayed-filled teeth/decayed-missing-filled teeth (DMFT) score was estimated according to the Brazilian Oral Health Project. The children were divided into two groups: caries-free group (DMFT=0, n=55) and caries experience group (DMFT≥1, n=131). Saliva was collected from each child using the spitting method and analyzed for stimulated saliva flow (SSF), pH, and iron levels. The SSF, pH, and salivary iron levels were analyzed for statistical assumptions of normality and homoscedasticity and compared between groups. All of the statistical tests were performed using R and SPSS software, with a level of significance of 0.05.RESULTS: No statistically significant differences in mean pH were found between groups (t=-0.0488, p=0.51). Children with caries experience had significantly lower salivary iron levels than caries-free children (w=5088, p<0.0001). The SSF values were significantly different between children with caries experience and children without caries (w=4198, p=0.03).CONCLUSION: Salivary iron levels were significantly lower in children with dental caries experience, suggesting that salivary iron plays a role in maintaining oral health.

scholarly journals Oral health status of children with type 1 diabetes: a comparative study

2017 ◽  
Vol 30 (11) ◽  
Author(s):  
Ahmad Faisal Ismail ◽  
Colman Patrick McGrath ◽  
Cynthia K.Y. Yiu
Keyword(s):  
Type 1 Diabetes ◽  
Health Status ◽  
Oral Health ◽  
Dental Caries ◽  
Comparative Study ◽  
Oral Health Status ◽  
Caries Experience ◽  
Health Examination ◽  
Significant Difference

AbstractBackground:The aim of this study was to compare the oral health status of children with type 1 diabetes and healthy controls.Methods:This comparative study involved 64 children, 32 children with type 1 diabetes and 32 age- and gender-matched controls. Oral health examination was conducted using WHO criteria. Dental caries experience was recorded using DMFT/dmft index and periodontal parameters were assessed using plaque, gingivitis, gingival bleeding and calculus indexes. Dental caries and periodontal parameters between the two groups were compared using the Mann-Whitney U-test.Results:Children with diabetes exhibited significantly greater plaque deposits (p=0.01) and a higher mean plaque index (p<0.01), when compared to healthy subjects. No significant difference in DMFT and dmft scores, mean bleeding index, calculus index and gingival index was found between the two groups.Conclusions:Children with type 1 diabetes had a poor oral health status with greater plaque accumulation than children without diabetes.

scholarly journals Physical and mental growth and development in children with congenital hypothyroidism: a case–control study

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Javad Nazari ◽  
Kimia Jafari ◽  
Maryam Chegini ◽  
Akram Maleki ◽  
Pari MirShafiei ◽  
...  
Keyword(s):  
Congenital Hypothyroidism ◽  
Case Control Study ◽  
Screening Program ◽  
Case Control ◽  
Healthy Children ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Children Development ◽  
Control Study

Abstract Introduction The clinical complications of congenital hypothyroidism such as brain disorders are very subtle and are not recognizable in infancy period. They are recognizable when it is too late for treatment or prevention. General screening of newborns is effective in diagnosing congenital hypothyroidism and initiating initial treatment. The aim of this study is to compare the physical and mental growth pattern of children with congenital hypothyroidism with healthy ones. Methods This case–control study was performed on 34 patients and 68 healthy children who were matched in terms of inclusion and exclusion criteria. Children development screening test (ASQ), children development assessment test (Bayley), preschool Wechsler intelligence scale (WPPSI) and age and steps questionnaire of emotional social development (ASQ-SE) were completed by trained questioners. Data were analyzed using STATA software. Results The results indicated that there was no significant difference between the mean of verbal (P = 0.77), non-verbal (P = 0.81) and general (P = 0.66) IQ in permanent and transient patients and healthy individuals. Also, there was no significant difference between the mean of different ranges of ASQ test (including communication, delicate and large movements, problem solving and social) at 12 months and 42 months (P < 0.05). According to Bayley test, there was no significant difference between the cases (permanent and transient) and controls in the cognitive (P = 0.42) and expressive (P = 0.38) categories. The difference was significant in the perceptual (P = 0.011), large (P = 0.03) and delicate (P = 0.04) movements categories. Conclusion This study emphasized on the high effectiveness of neonate hypothyroidism screening program, so that the difference between 3.5 years old children with and without this disease has decreased significantly. Early diagnosis of the patients, while creating beneficial effects for patients and increasing quality of life, cause reduction in the long-term costs of the health system.

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