DNA Repair Processes and Sensitivity to DNA Damage of Human Lymphocytes and Blasts: Analysis at the Single-Cell Level

1995 ◽  
pp. 155-161
Author(s):  
Claudia Buschfort ◽  
Frank Seiler ◽  
Mark R. Müller ◽  
Siegfried Seeber ◽  
Manfred F. Rajewsky ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Single Cell ◽  
Human Lymphocytes ◽  
Single Cell Level ◽  
Cell Level ◽  
Repair Processes

scholarly journals PLEK2 Gene Upregulation Might Independently Predict Shorter Progression-Free Survival in Lung Adenocarcinoma

2020 ◽  
Vol 19 ◽  
pp. 153303382095703
Author(s):  
Wenqian Zhang ◽  
Tong Li ◽  
Bin Hu ◽  
Hui Li
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Dna Repair ◽  
Lung Adenocarcinoma ◽  
Single Cell ◽  
Prognostic Value ◽  
Progression Free Survival ◽  
Single Cell Level ◽  
Cell Level ◽  
Free Survival

Objective: This study aimed to explore PLEK2 expression profile, its prognostic value, and the potential genomic alterations associated with its dysregulation in lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). Materials and methods: Data from The Cancer Genome Atlas (TCGA), The Genotype-Tissue Expression (GTEx), and Kaplan-Meier plotter were used in combination for bioinformatic analysis. Results: PLEK2 mRNA was significantly upregulated in both LUAD and LUSC compared with their respective normal controls. PLEK2 upregulation showed independent prognostic value in progression-free survival (PFS) (HR: 1.169, 95%CI: 1.033 -1.322, p = 0.014). PLEK2 mRNA expression was positively correlated with invasion, cell cycle, DNA damage, and DNA repair of LUAD cells at the single-cell level. Genomic analysis showed that gene-level amplification might not directly lead to increased PLEK2 expression. Methylation profile analysis found 4 CpG sites (cg12199376, cg14437634, cg17641252, and cg06724236) had at least a weakly negative correlation with PLEK2 expression, among which cg12199376, cg14437634 and cg17641252 locate around the first exon of the gene. Conclusions: Increased PLEK2 expression might be a specific prognostic biomarker of poor PFS in LUAD patients. Its expression had significant positive correlations with invasion, cell cycle, DNA damage, and DNA repair of LUAD cells at the single-cell level. Promoter hypomethylation might be a potential mechanism leading to its upregulation.

scholarly journals Genome-wide single-cell-level screen for protein abundance and localization changes in response to DNA damage in S. cerevisiae

2013 ◽  
Vol 41 (20) ◽  
pp. 9310-9324 ◽  
Author(s):  
Aprotim Mazumder ◽  
Laia Quiros Pesudo ◽  
Siobhan McRee ◽  
Mark Bathe ◽  
Leona D. Samson
Keyword(s):  
Dna Damage ◽  
Single Cell ◽  
Protein Abundance ◽  
Single Cell Level ◽  
Cell Level ◽  
Genome Wide

scholarly journals Spatial Dependence of DNA Damage in Bacteria due to Low-Temperature Plasma Application as Assessed at the Single Cell Level

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Angela Privat-Maldonado ◽  
Deborah O’Connell ◽  
Emma Welch ◽  
Roddy Vann ◽  
Marjan W. van der Woude
Keyword(s):  
Dna Damage ◽  
Low Temperature ◽  
Single Cell ◽  
Spatial Dependence ◽  
Single Cell Level ◽  
Low Temperature Plasma ◽  
Cell Level ◽  
Temperature Plasma

scholarly journals Repair of O6-alkylguanines in the nuclear DNA of human lymphocytes and leukaemic cells: analysis at the single-cell level

1994 ◽  
Vol 69 (4) ◽  
pp. 698-705 ◽  
Author(s):  
J Thomale ◽  
F Seiler ◽  
MR Müller ◽  
S Seeber ◽  
MF Rajewsky
Keyword(s):  
Single Cell ◽  
Nuclear Dna ◽  
Human Lymphocytes ◽  
Single Cell Level ◽  
Cell Level ◽  
Leukaemic Cells
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