Gut Regulatory Peptide Profiles in Acute Gastroenteritis and Necrotising Enterocolitis in Infancy

2015 ◽  
pp. 207-215
Author(s):  
A. Aynsley-Green ◽  
A. Lucas ◽  
M. Ghatei ◽  
G. R. Lawson ◽  
S. R. Bloom
Keyword(s):  
Acute Gastroenteritis ◽  
Necrotising Enterocolitis ◽  
Regulatory Peptide ◽  
Peptide Profiles

A Sensitive On-Grid Immunoelectron Microscopic Procedure for Diagnosis of Rotavirus Infection

1980 ◽  
Vol 38 ◽  
pp. 506-507
Author(s):  
M. F. Miller ◽  
A. R. Rubenstein
Keyword(s):  
Electron Microscopy ◽  
Specific Antibody ◽  
Immune Complexes ◽  
Acute Gastroenteritis ◽  
Plant Viruses ◽  
Aggregation Process ◽  
Microscopic Technique ◽  
Fecal Material ◽  
Present Communication ◽  
Number Of Particles

Studies of rotavirus particles in humans, monkeys and various non-primates with acute gastroenteritis have involved detection of virus in fecal material by electron microscopy. The EM techniques most commonly employed have been the conventional negative staining (Fig. 1) and immune aggregation (Fig. 2) procedures. Both methods are somewhat insensitive and can most reliably be applied to samples containing large quantities of virus either naturaLly or as a result of concentration by ultracentrifugation. The formation of immune complexes by specific antibody in the immune aggregation procedures confirms the rotavirus diagnosis, but the number of particles per given microscope field is effectively reduced by the aggregation process. In the present communication, we describe use of an on-grid immunoelectron microscopic technique in which rotavirus particles are mounted onto microscope grids that were pre-coated with specific antibody. The technique is a modification of a method originalLy introduced by Derrick (1) for studies of plant viruses.

Assessment of PCR real time for quantification of human enterovirus in children with acute gastroenteritis in Italy

2018 ◽  
Vol 30 (4) ◽  
Author(s):  
Valentina Daprà ◽  
Ilaria Galliano ◽  
Cristina Calvi ◽  
Paola Montanari ◽  
Massimiliano Bergallo
Keyword(s):  
Real Time ◽  
Acute Gastroenteritis ◽  
Human Enterovirus
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