Use of Serological Antibody Tests in Celiac Disease

2015 ◽  
pp. 108-129 ◽  
Author(s):  
Markku M�ki
Keyword(s):  
Celiac Disease ◽  
Antibody Tests

scholarly journals Serologic Testing for Celiac Disease in the United States: Results of a Multilaboratory Comparison Study

2000 ◽  
Vol 7 (4) ◽  
pp. 584-587 ◽  
Author(s):  
Joseph A. Murray ◽  
Judith Herlein ◽  
Frank Mitros ◽  
James A. Goeken
Keyword(s):  
United States ◽  
Celiac Disease ◽  
Sensitivity And Specificity ◽  
Immunoglobulin A ◽  
The United States ◽  
Comparison Study ◽  
Serologic Testing ◽  
Antibody Tests ◽  
Untreated Celiac Disease ◽  
Antigliadin Antibody

ABSTRACT The aim of this study was to compare the efficiencies of six reference laboratories for serologic testing for celiac disease. Serum from 20 patients with untreated celiac disease and from 20 controls was thawed, divided, and distributed to each participating laboratory, which performed endomysial antibody tests. Five laboratories also performed antigliadin antibody tests. Sensitivity for endomysial antibody immunoglobulin A (IgA) varied from 57 to 90%. In all laboratories, the specificity for celiac disease was 100%. The sensitivity and specificity for both IgA and IgG antigliadin antibody varied significantly. When results from all three tests were combined in each laboratory, sensitivity was 90 to 100%. The specificity for endomysial antibody was 100% in the laboratories. Sensitivity was less than reported previously. Standardization of these tests is needed in the United States.

scholarly journals Survey of the initial management of celiac disease antibody tests by ordering physicians

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Kathryn Potter ◽  
Lawrence de Koning ◽  
J. Decker Butzner ◽  
Dominica Gidrewicz
Keyword(s):  
Celiac Disease ◽  
Initial Management ◽  
Disease Antibody ◽  
Antibody Tests

scholarly journals Celiac Disease and Immunoglobulin A Deficiency: How Effective Are the Serological Methods of Diagnosis?

2002 ◽  
Vol 9 (6) ◽  
pp. 1295-1300 ◽  
Author(s):  
V. Kumar ◽  
M. Jarzabek-Chorzelska ◽  
J. Sulej ◽  
Krystyna Karnewska ◽  
T. Farrell ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Pediatric Patients ◽  
Tissue Transglutaminase ◽  
False Negative ◽  
Immunoglobulin A ◽  
Iga Deficiency ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Asymptomatic Patients ◽  
Antibody Tests

ABSTRACT Immunoglobulin A (IgA) deficiency is 10 to 15 times more common in patients with celiac disease (CD) than in healthy subjects. Serological tests have become the preferred methods of diagnosing CD in both symptomatic and asymptomatic patients. However, commercially available serological methods are limited in that they detect only the IgA isotype of antibodies (with the exception of IgG gliadin assays); hence, IgA-deficient patients with CD may yield false-negative serology. Fifteen pediatric patients with CD and 10 IgA-deficient pediatric patients without CD were examined for IgA and IgG antibodies to endomysium, gliadin, and tissue transglutaminase. Twenty-five specimens from patients with IgA deficiency were examined. Fifteen were from patients with CD, and 10 were patients without CD. All 15 IgA-deficient patients with CD were positive for endomysium antibodies of the IgG isotype and for IgG gliadin antibodies. All but one of the IgA-deficient patients with CD were also positive for IgG tissue transglutaminase antibodies. None of the IgA-deficient patients without CD were positive for any of the antibody markers. All the specimens examined were also negative for IgA-specific antibodies to endomysium, gliadin, and tissue transglutaminase. IgG-specific antibody tests for endomysium, gliadin, and tissue transglutaminase are useful for the identification of IgA-deficient patients with CD. IgG antibody tests along with tests routinely being used in clinical laboratories can reliably detect all active patients with CD. In addition, the levels of these CD-specific IgG antibodies could be used to monitor patient dietary compliance.

scholarly journals Serological Testing in Screening for Adult Celiac Disease

1999 ◽  
Vol 13 (3) ◽  
pp. 265-269 ◽  
Author(s):  
Helen Rachel Gillett ◽  
Hugh James Freeman
Keyword(s):  
Celiac Disease ◽  
High Risk ◽  
Small Bowel ◽  
Clinical Use ◽  
Serological Testing ◽  
First Line ◽  
Small Bowel Biopsy ◽  
Atypical Symptoms ◽  
Adult Celiac Disease ◽  
Antibody Tests

Assays for celiac-related antibodies are becoming widely available, and the present review aims to clarify the use of these investigations in the diagnosis of, management of and screening for adult celiac disease. The sensitivities and specificities of various antibody tests are discussed, along with their clinical use as an adjunct to small bowel biopsy, and as a first-line investigation for patients with atypical symptoms of celiac disease or patients at high risk of developing sprue.

Orally Based Diagnosis of Celiac Disease: Current Perspectives

2008 ◽  
Vol 87 (12) ◽  
pp. 1100-1107 ◽  
Author(s):  
L. Pastore ◽  
G. Campisi ◽  
D. Compilato ◽  
L. Lo Muzio
Keyword(s):  
Celiac Disease ◽  
Intestinal Mucosa ◽  
Tissue Transglutaminase ◽  
Wheat Gluten ◽  
Screening Tests ◽  
Small Intestinal Mucosa ◽  
Immune Mediated ◽  
Life Threatening ◽  
Histopathologic Findings ◽  
Antibody Tests

Celiac disease (CD) is a lifelong immune-mediated disorder caused by the ingestion of wheat gluten in genetically susceptible persons. Most cases of CD are atypical and remain undiagnosed, which exposes the individuals to the risk of life-threatening complications. Serologic endomysial and tissue transglutaminase antibody tests are used to screen at-risk individuals, although a firm diagnosis requires demonstration of characteristic histopathologic findings in the small-intestinal mucosa. A gluten challenge, with a repeat biopsy to demonstrate recurrence of histopathologic changes in the intestinal mucosa after the re-introduction of gluten, is considered for those persons in whom diagnosis remains in doubt. In this paper, we review studies that evaluated: (1) the possibility of using oral mucosa for the initial diagnosis of CD or for local gluten challenge; and (2) the possibility of using salivary CD-associated antibodies as screening tests. Our review shows that orally based diagnosis of CD is attractive and promising, although additional evaluations with standardized collection and analysis methods are needed. There is some evidence of a dissociation between systemic and oral mucosal immune responses in CD. The hypothesis that gluten could stimulate naïve lymphocytes directly in the oral cavity would have important implications for the understanding, diagnosis, and management of CD.

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