scholarly journals Comparison of Tissue Transglutaminase and Anti-Endomysial Antibody Tests in Diagnosis of Celiac Disease

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammad Sobhani Shahmirzadi ◽  
Ahmad Sohrabi
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Antibody Tests

scholarly journals Celiac Disease and Immunoglobulin A Deficiency: How Effective Are the Serological Methods of Diagnosis?

2002 ◽  
Vol 9 (6) ◽  
pp. 1295-1300 ◽  
Author(s):  
V. Kumar ◽  
M. Jarzabek-Chorzelska ◽  
J. Sulej ◽  
Krystyna Karnewska ◽  
T. Farrell ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Pediatric Patients ◽  
Tissue Transglutaminase ◽  
False Negative ◽  
Immunoglobulin A ◽  
Iga Deficiency ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Asymptomatic Patients ◽  
Antibody Tests

ABSTRACT Immunoglobulin A (IgA) deficiency is 10 to 15 times more common in patients with celiac disease (CD) than in healthy subjects. Serological tests have become the preferred methods of diagnosing CD in both symptomatic and asymptomatic patients. However, commercially available serological methods are limited in that they detect only the IgA isotype of antibodies (with the exception of IgG gliadin assays); hence, IgA-deficient patients with CD may yield false-negative serology. Fifteen pediatric patients with CD and 10 IgA-deficient pediatric patients without CD were examined for IgA and IgG antibodies to endomysium, gliadin, and tissue transglutaminase. Twenty-five specimens from patients with IgA deficiency were examined. Fifteen were from patients with CD, and 10 were patients without CD. All 15 IgA-deficient patients with CD were positive for endomysium antibodies of the IgG isotype and for IgG gliadin antibodies. All but one of the IgA-deficient patients with CD were also positive for IgG tissue transglutaminase antibodies. None of the IgA-deficient patients without CD were positive for any of the antibody markers. All the specimens examined were also negative for IgA-specific antibodies to endomysium, gliadin, and tissue transglutaminase. IgG-specific antibody tests for endomysium, gliadin, and tissue transglutaminase are useful for the identification of IgA-deficient patients with CD. IgG antibody tests along with tests routinely being used in clinical laboratories can reliably detect all active patients with CD. In addition, the levels of these CD-specific IgG antibodies could be used to monitor patient dietary compliance.

Orally Based Diagnosis of Celiac Disease: Current Perspectives

2008 ◽  
Vol 87 (12) ◽  
pp. 1100-1107 ◽  
Author(s):  
L. Pastore ◽  
G. Campisi ◽  
D. Compilato ◽  
L. Lo Muzio
Keyword(s):  
Celiac Disease ◽  
Intestinal Mucosa ◽  
Tissue Transglutaminase ◽  
Wheat Gluten ◽  
Screening Tests ◽  
Small Intestinal Mucosa ◽  
Immune Mediated ◽  
Life Threatening ◽  
Histopathologic Findings ◽  
Antibody Tests

Celiac disease (CD) is a lifelong immune-mediated disorder caused by the ingestion of wheat gluten in genetically susceptible persons. Most cases of CD are atypical and remain undiagnosed, which exposes the individuals to the risk of life-threatening complications. Serologic endomysial and tissue transglutaminase antibody tests are used to screen at-risk individuals, although a firm diagnosis requires demonstration of characteristic histopathologic findings in the small-intestinal mucosa. A gluten challenge, with a repeat biopsy to demonstrate recurrence of histopathologic changes in the intestinal mucosa after the re-introduction of gluten, is considered for those persons in whom diagnosis remains in doubt. In this paper, we review studies that evaluated: (1) the possibility of using oral mucosa for the initial diagnosis of CD or for local gluten challenge; and (2) the possibility of using salivary CD-associated antibodies as screening tests. Our review shows that orally based diagnosis of CD is attractive and promising, although additional evaluations with standardized collection and analysis methods are needed. There is some evidence of a dissociation between systemic and oral mucosal immune responses in CD. The hypothesis that gluten could stimulate naïve lymphocytes directly in the oral cavity would have important implications for the understanding, diagnosis, and management of CD.

Tissue transglutaminase, the celiac disease autoantigen, preferentially generates antigenic neoepitopes in γ and γ but not in α gliadins

2001 ◽  
Vol 120 (5) ◽  
pp. A684-A684
Author(s):  
D TRAPP ◽  
W DIETERICH ◽  
H WIESER ◽  
M LEIDENBERGER ◽  
D SEILMEIER ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase

245 THE ORAL TISSUE TRANSGLUTAMINASE INHIBITOR ZED1227 PREVENTS GLUTEN-INDUCED ENTEROPATHY IN A TRANSGENIC MOUSE MODEL OF CELIAC DISEASE

2021 ◽  
Vol 160 (6) ◽  
pp. S-53-S-54
Author(s):  
Manuel A. Encalada Ventura ◽  
Martin Hils ◽  
Bernhard Tewes ◽  
Ralf Pasternack ◽  
Roland Greinwald ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Tissue Transglutaminase ◽  
Transgenic Mouse Model ◽  
Oral Tissue

Tu1386 Susceptibility for Celiac Disease Based on Tissue Transglutaminase Seroprevalence and HLA Genotype in a Community Study

2016 ◽  
Vol 150 (4) ◽  
pp. S891
Author(s):  
Michael D. Potter ◽  
Gregory Brogan ◽  
Marjorie M. Walker ◽  
Mark McEvoy ◽  
Elizabeth Holliday ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Community Study ◽  
Hla Genotype

scholarly journals Effect of Rothia mucilaginosa enzymes on gliadin (gluten) structure, deamidation, and immunogenic epitopes relevant to celiac disease

2014 ◽  
Vol 307 (8) ◽  
pp. G769-G776 ◽  
Author(s):  
Na Tian ◽  
Guoxian Wei ◽  
Detlef Schuppan ◽  
Eva J. Helmerhorst
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Mass Difference ◽  
Cross Linking ◽  
Esi Ms ◽  
Rp Hplc ◽  
Complementary Approach ◽  
Sds Page ◽  
Rothia Mucilaginosa ◽  
Immunogenic Properties

Rothia mucilaginosa, a natural microbial inhabitant of the oral cavity, cleaves gluten (gliadin) proteins at regions that are resistant to degradation by mammalian enzymes. The aim of this study was to investigate to what extent the R. mucilaginosa cell-associated enzymes abolish gliadin immunogenic properties. Degradation of total gliadins and highly immunogenic gliadin 33-mer or 26-mer peptides was monitored by SDS-PAGE and RP-HPLC, and fragments were sequenced by liquid chromatography and electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). Peptide deamidation by tissue transglutaminase (TG2), a critical step in rendering the fragments more immunogenic, was assessed by TG2-mediated cross-linking to monodansyl cadaverine (MDC), and by a +1-Da mass difference by LC-ESI-MS. Survival of potential immunogenic gliadin epitopes was determined by use of the R5 antibody-based ELISA. R. mucilaginosa-associated enzymes cleaved gliadins, 33-mer and 26-mer peptides into smaller fragments. TG2-mediated cross-linking showed a perfect inverse relationship with intact 33-mer and 26-mer peptide levels, and major degradation fragments showed a slow rate of MDC cross-linking of 6.18 ± 2.20 AU/min compared with 97.75 ± 10.72 and 84.17 ± 3.25 AU/min for the intact 33-mer and 26-mer, respectively, which was confirmed by reduced TG2-mediated deamidation of the fragments in mass spectrometry. Incubation of gliadins with Rothia cells reduced R5 antibody binding by 20, 82, and 97% after 30 min, 2 h, and 5 h, respectively, which was paralleled by reduced reactivity of enzyme-treated 33-mer and 26-mer peptides in the R5 competitive ELISA. Our broad complementary approach to validate gluten degrading activities qualifies R. mucilaginosa-associated enzymes as promising tools to neutralize T cell immunogenic properties for treatment of celiac disease.

scholarly journals INCREASED TISSUE TRANSGLUTAMINASE LEVELS ARE ASSOCIATED WITH INCREASED EPILEPTIFORM ACTIVITY IN ELECTROENCEPHALOGRAPHY AMONG PATIENTS WITH CELIAC DISEASE

2015 ◽  
Vol 52 (4) ◽  
pp. 272-277 ◽  
Author(s):  
Sedat IŞIKAY ◽  
Şamil HIZLI ◽  
Serkan ÇOŞKUN ◽  
Kutluhan YILMAZ
Keyword(s):  
Celiac Disease ◽  
Tissue Transglutaminase ◽  
Pearson Correlation ◽  
Epileptiform Activity ◽  
Control Group ◽  
Healthy Children ◽  
Newly Diagnosed ◽  
Laboratory Findings ◽  
Significant Difference ◽  
Medical Histories

Background - Celiac disease is an autoimmune systemic disorder in genetically predisposed individuals precipitated by gluten ingestion. Objective - In this study, we aimed to determine asymptomatic spike-and-wave findings on electroencephalography in children with celiac disease. Methods - A total of 175 children with the diagnosis of celiac disease (study group) and 99 age- and sex-matched healthy children as controls (control group) were included in the study. In order to determine the effects of gluten free diet on laboratory and electroencephalography findings, the celiac group is further subdivided into two as newly-diagnosed and formerly-diagnosed patients. Medical histories of all children and laboratory findings were all recorded and neurologic statuses were evaluated. All patients underwent a sleep and awake electroencephalography. Results - Among 175 celiac disease patients included in the study, 43 were newly diagnosed while 132 were formerly-diagnosed patients. In electroencephalography evaluation of patients the epileptiform activity was determined in 4 (9.3%) of newly diagnosed and in 2 (1.5%) of formerly diagnosed patients; on the other hand the epileptiform activity was present in only 1 (1.0%) of control cases. There was a statistically significant difference between groups in regards to the presence of epileptiform activity in electroencephalography. Pearson correlation analysis revealed that epileptiform activity in both sleep and awake electroencephalography were positively correlated with tissue transglutaminase levels (P=0.014 and P=0.019, respectively). Conclusion - We have determined an increased epileptiform activity frequency among newly-diagnosed celiac disease patients compared with formerly-diagnosed celiac disease patients and control cases. Moreover the tissue transglutaminase levels were also correlated with the presence of epileptiform activity in electroencephalography. Among newly diagnosed celiac disease patients, clinicians should be aware of this association and be alert about any neurological symptoms.

Relationships between Clinical Presentation, Serology, Histology, and Duodenal Deposits of Tissue Transglutaminase Antibodies in Pediatric Celiac Disease

2018 ◽  
Vol 36 (5) ◽  
pp. 369-376 ◽  
Author(s):  
Nurit Loberman-Nachum ◽  
Michael Schvimer ◽  
Camila Avivi ◽  
Iris Barshack ◽  
Avishay Lahad ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Immunohistochemical Staining ◽  
Clinical Presentation ◽  
Tissue Transglutaminase ◽  
Mucosal Damage ◽  
High Serum ◽  
Tissue Transglutaminase Antibodies ◽  
Antibody Levels ◽  
Age Range ◽  
Multiple Symptoms

Background: The clinical, histological, and serological spectrum of celiac disease (CD) vary widely. We aimed to examine relationships between symptoms, serum anti-tissue transglutaminase antibodies (tTG) levels, mucosal damage, and mucosal anti-tTG deposits in pediatric CD. Methods: A retrospective single-center, cohort study of children referred for endoscopy with suspected CD during 2011–2014. We retrieved the clinical data, blindly reviewed duodenal biopsies, and performed immunohistochemical staining for anti-tTG deposits. Patients were classified as monosymptomatic or polysymptomatic. Mucosal anti-tTG deposits were classified according to the location of deposits, dominant intensity, maximal intensity, and percentage of stained area. Results: Of 252 patients with confirmed CD, complete data were available for 100: 37 males in the age range 1.3–16.7 with median 4.0 years. Monosymptomatic patients (n = 54) presented at an older age than polysymptomatic patients (1.3–15.5, median 8.1 vs. 1.3–16.7, median 6.3 years, p = 0.026). Marsh 2–3c was more prevalent in polysymptomatic patients (93 vs. 78%, p = 0.028). The intensity of mucosal anti-tTG deposits correlated with serum anti-tTG levels but not with the clinical presentation. Conclusions: Multiple symptoms and high serum anti-tTG antibody levels correlated with mucosal damage in children with CD. The role of immunohistochemical staining for intestinal anti-tTG mucosal deposits in the diagnosis of borderline CD is not yet established.

scholarly journals Screening Tests Using Serum Tissue Transglutaminase IgA May Facilitate the Identification of Undiagnosed Celiac Disease among Japanese Population

2014 ◽  
Vol 11 (8) ◽  
pp. 819-823 ◽  
Author(s):  
Hideyuki Nakazawa ◽  
Hideki Makishima ◽  
Toshiro Ito ◽  
Hiroyoshi Ota ◽  
Kayoko Momose ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Japanese Population ◽  
Tissue Transglutaminase ◽  
Screening Tests
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